GSE317529 eCLIP Data Processing

eCLIP geo_data_processing 2 steps

Publication

MBNL2 dysfunction in outer radial glial cells is associated with disrupted corticogenesis in congenital myotonic dystrophy.

Neurobiology of disease (2026) — PMID 41644016

Dataset

GSE317529

MBNL2 dysfunction in outer radial glial cells is associated with disrupted corticogenesis in congenital myotonic dystrophy

Warning: Pipeline descriptions and code snippets may be inferred or AI-generated. Use them only as a starting point to guide analysis, and validate before use.

Processing Steps

Generate Jupyter Notebook
  1. 1

    Libraries were sequenced to a minimum of 20 million SE100 reads per replicate using a NovaSeq X Plus (Illumina). eCLIP data were analyzed using Skipper (Boyle et al., Cell Genom, 2023).

    Skipper
  2. 2

    Reads were aligned to the hg38 reference genome and annotated with GENCODE v38.

    GENCODE

Tools Used

Skipper
Raw Source Text 
Libraries were sequenced to a minimum of 20 million SE100 reads per replicate using a NovaSeq X Plus (Illumina). eCLIP data were analyzed using Skipper (Boyle et al., Cell Genom, 2023). Reads were aligned to the hg38 reference genome and annotated with GENCODE v38.
Assembly: hg38
Supplementary files format and content: Tab-separated values files containing enriched eCLIP binding windows
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