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GSE196177

GSE GEO
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Nanostring based analysis of CBF-AML patients

Organism: Homo sapiens
Platform: GPL26945
Samples: 48
Experiment Types:
Other
Submitted: Feb 04 2022
Last Updated: Jul 01 2024
Status: Public on Mar 08 2022
Contact: Alexander,James,Murison (University Health Network)

Relations

SubSeries of: GSE181140 BioProject: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA803535

Summary

miR-130a is captured as part of a 798 gene probe set on a microarray plate for 48 CBF patients In the group of patients defined here, survival characteristics and relapse information was collected to unbiasedly assess the impact of any miRNA on survival

Overall Design

Microarray analysis of peripheral blood blast samples - Nanostring

Analysis (4 steps)

View Data Processing
Processing steps for GSE196177
  1. The output of the assay was analyzed by nSolver 4.0 where the mean of the negative spike-in control was used as the threshold of microRNA detection.
  2. With the expression profile table generated, patients were then stratified by miR-130a expression using a median split and a Kaplan-Meier curve was drawn by GraphPad Prism 7.
  3. All counted reads are provided in the count matrix.
  4. Data normalization was conducted within the Ncounter software that used a median cut-off of the negative detection probes

Supplementary Files (2)

GSE196177_RAW.tar Download
GSE196177_normalized_data.txt.gz Download
GEO Samples (48)
GSM5862049 GSM5862050 GSM5862051 GSM5862052 GSM5862053 GSM5862054 GSM5862055 GSM5862056 GSM5862057 GSM5862058 GSM5862059 GSM5862060 GSM5862061 GSM5862062 GSM5862063 GSM5862064 GSM5862065 GSM5862066 GSM5862067 GSM5862068 GSM5862069 GSM5862070 GSM5862071 GSM5862072 GSM5862073 GSM5862074 GSM5862075 GSM5862076 GSM5862077 GSM5862078 GSM5862079 GSM5862080 GSM5862081 GSM5862082 GSM5862083 GSM5862084 GSM5862085 GSM5862086 GSM5862087 GSM5862088 GSM5862089 GSM5862090 GSM5862091 GSM5862092 GSM5862093 GSM5862094 GSM5862095 GSM5862096

Dataset Citations (1)

Identification of the global miR-130a targetome reveals a role for TBL1XR1 in hematopoietic stem cell self-renewal and t(8;21) AML.
PMID 35263585 · 2022 · Cell reports
Gabriela Krivdova, Veronique Voisin, Erwin M Schoof, Sajid A Marhon, Alex Murison, Jessica L McLeod, Martino M Gabra, Andy G X Zeng, Stefan Aigner, Brian A Yee, Alexander A Shishkin, Eric L Van Nostrand, Karin G Hermans, Aaron C Trotman-Grant, Nathan Mbong, James A Kennedy, Olga I Gan, Elvin Wagenblast, Daniel D De Carvalho, Leonardo Salmena, Mark D Minden, Gary D Bader, Gene W Yeo, John E Dick, Eric R Lechman

Linked Publications (1)

Identification of the global miR-130a targetome reveals a role for TBL1XR1 in hematopoietic stem cell self-renewal and t(8;21) AML.
Cell reports · 2022