GSE16681
GSE GEOmRNA expression data from differentiation of human ESCs into definitive endoderm, Cyt49 on matrigel
Organism:
Homo sapiens
Platform:
GPL7363
Samples:
9
Experiment Types:
Expression profiling by array
Submitted:
Jun 17 2009
Last Updated:
Jan 08 2019
Status:
Public on Jul 30 2010
Contact:
Andrew,,Hinton (UC, San Diego)
Relations
SubSeries of: GSE16690
BioProject: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA122719
Summary
hESCs can differentiate into the three primary embryonic lineages (endoderm, mesoderm, ectoderm) as well as extraembryonic tissues. Definitive endoderm (DE) is the first step into the pathway to endoderm derived tissues: pancreas, liver, gut, lung. We used microarrays to detail the changes in mRNA expression during the transition from pluripotent hESCs into definitive endoderm.
Overall Design
hESCs (Cyt49) were differentiated in the presence of Activin A and Wnt3A under low serum conditions to induce DE formation. Samples were collected at day 0, day 2 and day 4.
Analysis (1 step)
View Data Processing
Processing steps for GSE16681
- The data were normalised using quantile normalisation with IlluminaGUI in R
Supplementary Files (1)
GSE16681_non-normalized_data.txt.gz
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Dataset Citations (1)
A distinct microRNA signature for definitive endoderm derived from human embryonic stem cells.
PMID 19807270
· 2010
· Stem cells and development
Andrew Hinton, Ivka Afrikanova, Mike Wilson, Charles C King, Brian Maurer, Gene W Yeo, Alberto Hayek, Amy E Pasquinelli
Linked Publications (1)
A distinct microRNA signature for definitive endoderm derived from human embryonic stem cells.
Stem cells and development · 2010