Integrative genome-wide analysis reveals cooperative regulation of alternative splicing by hnRNP proteins (RNA-Seq)
Organism:
Homo sapiens
Platform:
GPL9115
Samples:
31
Experiment Types:
Expression profiling by high throughput sequencing
Submitted:
Jan 10 2012
Last Updated:
May 15 2019
Status:
Public on Feb 20 2012
Contact:
Gene,,Yeo (UCSD)
Relations
SubSeries of: GSE34996
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRP010280
BioProject: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA156297
Summary
Understanding how RNA binding proteins control the splicing code is fundamental to human biology and disease. Here we present a comprehensive study to elucidate how heterogeneous nuclear ribonucleoparticle (hnRNP) proteins, among the most abundant RNA binding proteins, coordinate to regulate alternative pre-mRNA splicing (AS) in human cells. Using splicing-sensitive microarrays, cross-linking and immunoprecipitation coupled with high-throughput sequencing, and cDNA sequencing, we find that more than half of all AS events are regulated by multiple hnRNP proteins, and that some combinations of hnRNP proteins exhibit significant synergy, whereas others act antagonistically. Our analyses reveal position-dependent RNA splicing maps, in vivo consensus binding sites, a surprising level of cross- and auto-regulation among hnRNP proteins, and the coordinated regulation by hnRNP proteins of dozens of other RNA binding proteins and genes associated with cancer. Our findings define an unprecedented degree of complexity and compensatory relationships among hnRNP proteins and their splicing targets that likely confer robustness to cells.
Overall Design
RNAseq for control, hnRNP A1, hnRNP A2/B1, hnRNP H1, hnRNP F, hnRNP M, and hnRNP U siRNA treated human 293T cells
SRA Experiments (31) and Runs (31)
Total: 22660 MB
GSM859993: hnRNPA1 293T RNAseq KD1a
Sample: SRS285637
BioProject: PRJNA156297
BioSample: SAMN00771634
Platform: ILLUMINA
Instrument: Illumina Genome Analyzer II
Organism: Homo sapiens
Sample attributes
source_name: human 293T cells
treatment: hnRNPA1 siRNAs
cell line: 293T
read length: 36
Original files (1)
Runs (1)
| Run |
Spots |
Bases |
Size (MB) |
Files |
Link |
| SRR398210 |
11347310 |
408503160 |
266.87 |
hnRNPA1_293T_RNAseq_KD1a_sequence.txt, SRR398210, SRR398210.sralite
|
SRA
|
GSM859994: hnRNPA1 293T RNAseq KD2a
Sample: SRS285638
BioProject: PRJNA156297
BioSample: SAMN00771635
Platform: ILLUMINA
Instrument: Illumina Genome Analyzer II
Organism: Homo sapiens
Sample attributes
source_name: human 293T cells
treatment: hnRNPA1 siRNAs
cell line: 293T
read length: 36
Original files (1)
Runs (1)
| Run |
Spots |
Bases |
Size (MB) |
Files |
Link |
| SRR398211 |
13368747 |
481274892 |
315.55 |
hnRNPA1_293T_RNAseq_KD2a_sequence.txt, SRR398211, SRR398211.sralite
|
SRA
|
GSM859995: hnRNPA1 293T RNAseq KD3a
Sample: SRS285639
BioProject: PRJNA156297
BioSample: SAMN00771636
Platform: ILLUMINA
Instrument: Illumina Genome Analyzer II
Organism: Homo sapiens
Sample attributes
source_name: human 293T cells
treatment: hnRNPA1 siRNAs
cell line: 293T
read length: 42
Original files (1)
Runs (1)
| Run |
Spots |
Bases |
Size (MB) |
Files |
Link |
| SRR398212 |
15561400 |
653578800 |
258.72 |
hnRNPA1_293T_RNAseq_KD3a_sequence.txt, SRR398212, SRR398212.sralite
|
SRA
|
GSM859996: hnRNPA1 293T RNAseq KD3b
Sample: SRS285640
BioProject: PRJNA156297
BioSample: SAMN00771637
Platform: ILLUMINA
Instrument: Illumina Genome Analyzer II
Organism: Homo sapiens
Sample attributes
source_name: human 293T cells
treatment: hnRNPA1 siRNAs
cell line: 293T
read length: 76
Original files (1)
Runs (1)
| Run |
Spots |
Bases |
Size (MB) |
Files |
Link |
| SRR398213 |
28489741 |
2165220316 |
1196.74 |
hnRNPA1_293T_RNAseq_KD3b_sequence.txt, SRR398213, SRR398213.sralite
|
SRA
|
GSM859998: hnRNPA1 293T RNAseq KD3c
Sample: SRS285641
BioProject: PRJNA156297
BioSample: SAMN00771638
Platform: ILLUMINA
Instrument: Illumina Genome Analyzer II
Organism: Homo sapiens
Sample attributes
source_name: human 293T cells
treatment: hnRNPA1 siRNAs
cell line: 293T
read length: 50
Original files (1)
Runs (1)
| Run |
Spots |
Bases |
Size (MB) |
Files |
Link |
| SRR398214 |
28447863 |
1422393150 |
760.08 |
hnRNPA1_293T_RNAseq_KD3c_sequence.txt, SRR398214, SRR398214.sralite
|
SRA
|
GSM859999: hnRNPA1 293T RNAseq KD3d
Sample: SRS285642
BioProject: PRJNA156297
BioSample: SAMN00771639
Platform: ILLUMINA
Instrument: Illumina Genome Analyzer II
Organism: Homo sapiens
Sample attributes
source_name: human 293T cells
treatment: hnRNPA1 siRNAs
cell line: 293T
read length: 50
Original files (1)
Runs (1)
| Run |
Spots |
Bases |
Size (MB) |
Files |
Link |
| SRR398215 |
28821723 |
1441086150 |
769.15 |
hnRNPA1_293T_RNAseq_KD3d_sequence.txt, SRR398215, SRR398215.sralite
|
SRA
|
GSM860001: hnRNPA2B1 293T RNAseq KD1a
Sample: SRS285643
BioProject: PRJNA156297
BioSample: SAMN00771640
Platform: ILLUMINA
Instrument: Illumina Genome Analyzer II
Organism: Homo sapiens
Sample attributes
source_name: human 293T cells
treatment: hnRNPA2B1 siRNAs
cell line: 293T
read length: 70
Original files (1)
Runs (1)
| Run |
Spots |
Bases |
Size (MB) |
Files |
Link |
| SRR398216 |
14825334 |
1037773380 |
629.86 |
hnRNPA2B1_293T_RNAseq_KD1a_sequence.txt, SRR398216, SRR398216.sralite
|
SRA
|
GSM860004: hnRNPA2B1 293T RNAseq KD2a
Sample: SRS285644
BioProject: PRJNA156297
BioSample: SAMN00771641
Platform: ILLUMINA
Instrument: Illumina Genome Analyzer II
Organism: Homo sapiens
Sample attributes
source_name: human 293T cells
treatment: hnRNPA2B1 siRNAs
cell line: 293T
read length: 70
Original files (1)
Runs (1)
| Run |
Spots |
Bases |
Size (MB) |
Files |
Link |
| SRR398217 |
11055019 |
773851330 |
447.58 |
hnRNPA2B1_293T_RNAseq_KD2a_sequence.txt, SRR398217, SRR398217.sralite
|
SRA
|
GSM860007: hnRNPF 293T RNAseq KD1a
Sample: SRS285645
BioProject: PRJNA156297
BioSample: SAMN00771642
Platform: ILLUMINA
Instrument: Illumina Genome Analyzer II
Organism: Homo sapiens
Sample attributes
source_name: human 293T cells
treatment: hnRNPF siRNA
cell line: 293T
read length: 50
Original files (1)
Runs (1)
| Run |
Spots |
Bases |
Size (MB) |
Files |
Link |
| SRR398218 |
20201080 |
1010054000 |
446.94 |
hnRNPF_293T_RNAseq_KD1a_sequence.txt, SRR398218, SRR398218.sralite
|
SRA
|
GSM860009: hnRNPF 293T RNAseq KD1b
Sample: SRS285646
BioProject: PRJNA156297
BioSample: SAMN00771643
Platform: ILLUMINA
Instrument: Illumina Genome Analyzer II
Organism: Homo sapiens
Sample attributes
source_name: human 293T cells
treatment: hnRNPF siRNA
cell line: 293T
read length: 50
Original files (1)
Runs (1)
| Run |
Spots |
Bases |
Size (MB) |
Files |
Link |
| SRR398219 |
26901083 |
1345054150 |
720.11 |
hnRNPF_293T_RNAseq_KD1b_sequence.txt, SRR398219, SRR398219.sralite
|
SRA
|
GSM860011: hnRNPF 293T RNAseq KD2a
Sample: SRS285647
BioProject: PRJNA156297
BioSample: SAMN00771644
Platform: ILLUMINA
Instrument: Illumina Genome Analyzer II
Organism: Homo sapiens
Sample attributes
source_name: human 293T cells
treatment: hnRNPF siRNA
cell line: 293T
read length: 50
Original files (1)
Runs (1)
| Run |
Spots |
Bases |
Size (MB) |
Files |
Link |
| SRR398220 |
23952003 |
1197600150 |
670.72 |
hnRNPF_293T_RNAseq_KD2a_sequence.txt, SRR398220, SRR398220.sralite
|
SRA
|
GSM860012: hnRNPF 293T RNAseq KD2b
Sample: SRS285648
BioProject: PRJNA156297
BioSample: SAMN00771645
Platform: ILLUMINA
Instrument: Illumina Genome Analyzer II
Organism: Homo sapiens
Sample attributes
source_name: human 293T cells
treatment: hnRNPF siRNA
cell line: 293T
read length: 50
Original files (1)
Runs (1)
| Run |
Spots |
Bases |
Size (MB) |
Files |
Link |
| SRR398221 |
29162696 |
1458134800 |
779.53 |
hnRNPF_293T_RNAseq_KD2b_sequence.txt, SRR398221, SRR398221.sralite
|
SRA
|
GSM860013: hnRNPF 293T RNAseq KD2c
Sample: SRS285649
BioProject: PRJNA156297
BioSample: SAMN00771646
Platform: ILLUMINA
Instrument: Illumina Genome Analyzer II
Organism: Homo sapiens
Sample attributes
source_name: human 293T cells
treatment: hnRNPF siRNA
cell line: 293T
read length: 50
Original files (1)
Runs (1)
| Run |
Spots |
Bases |
Size (MB) |
Files |
Link |
| SRR398222 |
28946624 |
1447331200 |
767.95 |
hnRNPF_293T_RNAseq_KD2c_sequence.txt, SRR398222, SRR398222.sralite
|
SRA
|
GSM860014: hnRNPH1 293T RNAseq KD1a
Sample: SRS285650
BioProject: PRJNA156297
BioSample: SAMN00771647
Platform: ILLUMINA
Instrument: Illumina Genome Analyzer II
Organism: Homo sapiens
Sample attributes
source_name: human 293T cells
treatment: hnRNPH1 siRNAs
cell line: 293T
read length: 70
Original files (1)
Runs (1)
| Run |
Spots |
Bases |
Size (MB) |
Files |
Link |
| SRR398223 |
16876308 |
1181341560 |
688.77 |
hnRNPH1_293T_RNAseq_KD1a_sequence.txt, SRR398223, SRR398223.sralite
|
SRA
|
GSM860015: hnRNPH1 293T RNAseq KD2a
Sample: SRS285651
BioProject: PRJNA156297
BioSample: SAMN00771648
Platform: ILLUMINA
Instrument: Illumina Genome Analyzer II
Organism: Homo sapiens
Sample attributes
source_name: human 293T cells
treatment: hnRNPH1 siRNAs
cell line: 293T
read length: 70
Original files (1)
Runs (1)
| Run |
Spots |
Bases |
Size (MB) |
Files |
Link |
| SRR398224 |
27222006 |
1905540420 |
1186.52 |
hnRNPH1_293T_RNAseq_KD2a_sequence.txt, SRR398224, SRR398224.sralite
|
SRA
|
GSM860016: hnRNPM 293T RNAseq KD1a
Sample: SRS285652
BioProject: PRJNA156297
BioSample: SAMN00771649
Platform: ILLUMINA
Instrument: Illumina Genome Analyzer II
Organism: Homo sapiens
Sample attributes
source_name: human 293T cells
treatment: hnRNPM siRNAs
cell line: 293T
read length: 36
Original files (1)
Runs (1)
| Run |
Spots |
Bases |
Size (MB) |
Files |
Link |
| SRR398225 |
14126599 |
508557564 |
340.18 |
hnRNPM_293T_RNAseq_KD1a_sequence.txt, SRR398225, SRR398225.sralite
|
SRA
|
GSM860017: hnRNPM 293T RNAseq KD1b
Sample: SRS285653
BioProject: PRJNA156297
BioSample: SAMN00771650
Platform: ILLUMINA
Instrument: Illumina Genome Analyzer II
Organism: Homo sapiens
Sample attributes
source_name: human 293T cells
treatment: hnRNPM siRNAs
cell line: 293T
read length: 76
Original files (1)
Runs (1)
| Run |
Spots |
Bases |
Size (MB) |
Files |
Link |
| SRR398226 |
28234868 |
2145849968 |
1173.42 |
hnRNPM_293T_RNAseq_KD1b_sequence.txt, SRR398226.sralite
|
SRA
|
GSM860018: hnRNPM 293T RNAseq KD1c
Sample: SRS285654
BioProject: PRJNA156297
BioSample: SAMN00771651
Platform: ILLUMINA
Instrument: Illumina Genome Analyzer II
Organism: Homo sapiens
Sample attributes
source_name: human 293T cells
treatment: hnRNPM siRNAs
cell line: 293T
read length: 50
Original files (1)
Runs (1)
| Run |
Spots |
Bases |
Size (MB) |
Files |
Link |
| SRR398227 |
26461030 |
1323051500 |
688.64 |
hnRNPM_293T_RNAseq_KD1c_sequence.txt, SRR398227, SRR398227.sralite
|
SRA
|
GSM860019: hnRNPM 293T RNAseq KD1d
Sample: SRS285655
BioProject: PRJNA156297
BioSample: SAMN00771652
Platform: ILLUMINA
Instrument: Illumina Genome Analyzer II
Organism: Homo sapiens
Sample attributes
source_name: human 293T cells
treatment: hnRNPM siRNAs
cell line: 293T
read length: 50
Original files (1)
Runs (1)
| Run |
Spots |
Bases |
Size (MB) |
Files |
Link |
| SRR398228 |
26345404 |
1317270200 |
684.92 |
hnRNPM_293T_RNAseq_KD1d_sequence.txt, SRR398228, SRR398228.sralite
|
SRA
|
GSM860020: hnRNPM 293T RNAseq KD2a
Sample: SRS285656
BioProject: PRJNA156297
BioSample: SAMN00771653
Platform: ILLUMINA
Instrument: Illumina Genome Analyzer II
Organism: Homo sapiens
Sample attributes
source_name: human 293T cells
treatment: hnRNPM siRNAs
cell line: 293T
read length: 36
Original files (1)
Runs (1)
| Run |
Spots |
Bases |
Size (MB) |
Files |
Link |
| SRR398229 |
13169724 |
474110064 |
324.68 |
hnRNPM_293T_RNAseq_KD2a_sequence.txt, SRR398229, SRR398229.sralite
|
SRA
|
GSM860021: hnRNPM 293T RNAseq KD2b
Sample: SRS285657
BioProject: PRJNA156297
BioSample: SAMN00771654
Platform: ILLUMINA
Instrument: Illumina Genome Analyzer II
Organism: Homo sapiens
Sample attributes
source_name: human 293T cells
treatment: hnRNPM siRNAs
cell line: 293T
read length: 76
Original files (1)
Runs (1)
| Run |
Spots |
Bases |
Size (MB) |
Files |
Link |
| SRR398230 |
6426395 |
488406020 |
320.61 |
hnRNPM_293T_RNAseq_KD2b_sequence.txt, SRR398230, SRR398230.sralite
|
SRA
|
GSM860022: hnRNPU 293T RNAseq KD1a
Sample: SRS285658
BioProject: PRJNA156297
BioSample: SAMN00771655
Platform: ILLUMINA
Instrument: Illumina Genome Analyzer II
Organism: Homo sapiens
Sample attributes
source_name: human 293T cells
treatment: hnRNPU siRNAs
cell line: 293T
read length: 50
Original files (1)
Runs (1)
| Run |
Spots |
Bases |
Size (MB) |
Files |
Link |
| SRR398231 |
20545025 |
1027251250 |
449.76 |
hnRNPU_293T_RNAseq_KD1a_sequence.txt, SRR398231, SRR398231.sralite
|
SRA
|
GSM860023: hnRNPU 293T RNAseq KD1b
Sample: SRS285659
BioProject: PRJNA156297
BioSample: SAMN00771656
Platform: ILLUMINA
Instrument: Illumina Genome Analyzer II
Organism: Homo sapiens
Sample attributes
source_name: human 293T cells
treatment: hnRNPU siRNAs
cell line: 293T
read length: 76
Original files (1)
Runs (1)
| Run |
Spots |
Bases |
Size (MB) |
Files |
Link |
| SRR398232 |
28384045 |
2157187420 |
1201.61 |
hnRNPU_293T_RNAseq_KD1b_sequence.txt, SRR398232, SRR398232.sralite
|
SRA
|
GSM860024: hnRNPU 293T RNAseq KD2a
Sample: SRS285660
BioProject: PRJNA156297
BioSample: SAMN00771657
Platform: ILLUMINA
Instrument: Illumina Genome Analyzer II
Organism: Homo sapiens
Sample attributes
source_name: human 293T cells
treatment: hnRNPU siRNAs
cell line: 293T
read length: 50
Original files (1)
Runs (1)
| Run |
Spots |
Bases |
Size (MB) |
Files |
Link |
| SRR398233 |
28463412 |
1423170600 |
762.16 |
hnRNPU_293T_RNAseq_KD2a_sequence.txt, SRR398233, SRR398233.sralite
|
SRA
|
GSM860025: hnRNPU 293T RNAseq KD2b
Sample: SRS285661
BioProject: PRJNA156297
BioSample: SAMN00771658
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: human 293T cells
treatment: hnRNPU siRNAs
cell line: 293T
read length: 50
Original files (1)
Runs (1)
| Run |
Spots |
Bases |
Size (MB) |
Files |
Link |
| SRR398234 |
116330730 |
5816536500 |
3278.67 |
hnRNPU_293T_RNAseq_KD2b_sequence.txt, SRR398234, SRR398234.sralite
|
SRA
|
GSM860026: control 293T RNAseq 1
Sample: SRS285662
BioProject: PRJNA156297
BioSample: SAMN00771659
Platform: ILLUMINA
Instrument: Illumina Genome Analyzer II
Organism: Homo sapiens
Sample attributes
source_name: human 293T cells
treatment: Control siRNA
cell line: 293T
read length: 50
Original files (1)
Runs (1)
| Run |
Spots |
Bases |
Size (MB) |
Files |
Link |
| SRR398235 |
22115682 |
1105784100 |
485.33 |
control_293T_RNAseq_1_sequence.txt, SRR398235, SRR398235.sralite
|
SRA
|
GSM860027: control 293T RNAseq 2
Sample: SRS285663
BioProject: PRJNA156297
BioSample: SAMN00771660
Platform: ILLUMINA
Instrument: Illumina Genome Analyzer II
Organism: Homo sapiens
Sample attributes
source_name: human 293T cells
treatment: Control siRNA
cell line: 293T
read length: 50
Original files (1)
Runs (1)
| Run |
Spots |
Bases |
Size (MB) |
Files |
Link |
| SRR398236 |
13005200 |
650260000 |
397.31 |
control_293T_RNAseq_2_sequence.txt, SRR398236, SRR398236.sralite
|
SRA
|
GSM860028: control 293T RNAseq 3
Sample: SRS285664
BioProject: PRJNA156297
BioSample: SAMN00771661
Platform: ILLUMINA
Instrument: Illumina Genome Analyzer II
Organism: Homo sapiens
Sample attributes
source_name: human 293T cells
treatment: Control siRNA
cell line: 293T
read length: 70
Original files (1)
Runs (1)
| Run |
Spots |
Bases |
Size (MB) |
Files |
Link |
| SRR398237 |
13940769 |
975853830 |
557.74 |
control_293T_RNAseq_3_sequence.txt, SRR398237, SRR398237.sralite
|
SRA
|
GSM860029: control 293T RNAseq 4
Sample: SRS285665
BioProject: PRJNA156297
BioSample: SAMN00771662
Platform: ILLUMINA
Instrument: Illumina Genome Analyzer II
Organism: Homo sapiens
Sample attributes
source_name: human 293T cells
treatment: Control siRNA
cell line: 293T
read length: 70
Original files (1)
Runs (1)
| Run |
Spots |
Bases |
Size (MB) |
Files |
Link |
| SRR398238 |
13577784 |
950444880 |
547.72 |
control_293T_RNAseq_4_sequence.txt, SRR398238, SRR398238.sralite
|
SRA
|
GSM860030: control 293T RNAseq 5
Sample: SRS285666
BioProject: PRJNA156297
BioSample: SAMN00771663
Platform: ILLUMINA
Instrument: Illumina Genome Analyzer II
Organism: Homo sapiens
Sample attributes
source_name: human 293T cells
treatment: Control siRNA
cell line: 293T
read length: 50
Original files (1)
Runs (1)
| Run |
Spots |
Bases |
Size (MB) |
Files |
Link |
| SRR398239 |
28467294 |
1423364700 |
751.52 |
control_293T_RNAseq_5_sequence.txt, SRR398239, SRR398239.sralite
|
SRA
|
GSM860031: control 293T RNAseq 6
Sample: SRS285667
BioProject: PRJNA156297
BioSample: SAMN00771664
Platform: ILLUMINA
Instrument: Illumina Genome Analyzer II
Organism: Homo sapiens
Sample attributes
source_name: human 293T cells
treatment: Control siRNA
cell line: 293T
read length: 50
Original files (1)
Runs (1)
| Run |
Spots |
Bases |
Size (MB) |
Files |
Link |
| SRR398240 |
29546883 |
1477344150 |
790.21 |
control_293T_RNAseq_6_sequence.txt, SRR398240, SRR398240.sralite
|
SRA
|