← All datasets

GSE78960

GSE GEO
View on GEO Export SRA CSV

Modeling the Neuropathology of Tuberous Sclerosis with Human Stem Cells Reveals a Role for Inflammation and Angiogenic Growth Factors [Treatment]

Organism: Homo sapiens
Platform: GPL16791
Samples: 48
Experiment Types:
Expression profiling by high throughput sequencing
Submitted: Mar 07 2016
Last Updated: Nov 08 2020
Status: Public on Jul 25 2016
Contact: Jitao David,,Zhang (F. Hoffmann-La Roche)

Relations

SubSeries of: GSE78961 BioProject: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA314507 SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRP071235

Summary

Tuberous sclerosis complex (TSC) is a rare genetic disease characterized by mTOR hyperfunction induced benign tumor growths in multiple organs and neurological symptoms. Because the molecular pathology is highly complex and the etiology poorly understood we employed a defined human neuronal model with a single mTOR activating mutation to dissect the disease-relevant molecular responses driving the neuropathology. TSC2 deficient neural stem cells showed severely reduced neuronal functional maturation and characteristics of astrogliosis instead. Accordingly, transcriptome analysis uncovered an inflammatory response and increased metabolic activity, while ribosome profiling revealed excessive translation of ribosomal transcripts and higher synthesis rates of angiogenic growth factors. Treatment with mTOR inhibitors corrected translational alterations but not transcriptional dysfunction. These results extend our understanding of the molecular pathophysiology of TSC brain lesions, and suggest phenotype-tailored pharmacological treatment strategies.

Overall Design

Rapamycin, AZD-8055, and DMSO were given to two TSC+/+ cell lines and two TSC-/- cell lines after six weeks of differentiation. Cells are harvested after 3 hours treatment and are subject to ribosome profiling and RNA-seq analysis.

Analysis (3 steps)

View Data Processing
Processing steps for GSE78960
  1. Linker tags were removed from RNA sequencing and ribosome profiling reads by the FASTX Toolkit, v0.0.13 (http://hannonlab.cshl.edu/fastx_toolkit/)
  2. All reads that mapped to rRNAs, tRNAs or mitochondrial rRNAs were removed, and the remaining reads were mapped to RefSeq (v38) by TopHat v2.0.13.
  3. Finally all read counts that mapped uniquely to genes were extracted for expression analysis with the help of samtools, v1.1.

Supplementary Files (2)

GSE78960_TSC2-treatment_RNA.txt.gz Download
GSE78960_TSC2-treatment_RPF.txt.gz Download
GEO Samples (48)
GSM2082533 GSM2082534 GSM2082535 GSM2082536 GSM2082537 GSM2082538 GSM2082539 GSM2082540 GSM2082541 GSM2082542 GSM2082543 GSM2082544 GSM2082545 GSM2082546 GSM2082547 GSM2082548 GSM2082549 GSM2082550 GSM2082551 GSM2082552 GSM2082553 GSM2082554 GSM2082555 GSM2082556 GSM2082557 GSM2082558 GSM2082559 GSM2082560 GSM2082561 GSM2082562 GSM2082563 GSM2082564 GSM2082565 GSM2082566 GSM2082567 GSM2082568 GSM2082569 GSM2082570 GSM2082571 GSM2082572 GSM2082573 GSM2082574 GSM2082575 GSM2082576 GSM2082577 GSM2082578 GSM2082579 GSM2082580

Dataset Citations (1)

Genomic analysis of the molecular neuropathology of tuberous sclerosis using a human stem cell model.
PMID 27655340 · 2016 · Genome medicine
Nils Grabole, Jitao David Zhang, Stefan Aigner, Nadine Ruderisch, Veronica Costa, Felix C Weber, Michel Theron, Nikolaos Berntenis, Olivia Spleiss, Martin Ebeling, Gene W Yeo, Ravi Jagasia, Anna Kiialainen

SRA Experiments (48) and Runs (48)

Total: 28570 MB
SRX1617564 SRP071235 RNA-Seq SINGLE
GSM2082533: Ribosome protected fragments (RPF) of TSC2+/+ cells (number 15) treated with AZD-8055, Biological Replicate 1; Homo sapiens; RNA-Seq
Sample: SRS1326996
BioProject: PRJNA314507
BioSample: SAMN04536284
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2+/+ cells (number 15) treated with AZD-8055, RPF
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2+/+
treatment: AZD-8055
cell line id: 15
assayed molecule: Ribosome protected fragments
Original files (1)
TSC2+/+ cells (number 15) treated with AZD-8055, RPF
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208881 27119422 1383090522 831.45 15AZD_ATCACG_L001_R1_001.fastq.gz, SRR3208881, SRR3208881.sralite SRA
SRX1617565 SRP071235 RNA-Seq SINGLE
GSM2082534: Ribosome protected fragments (RPF) of TSC2+/+ cells (number 15) treated with AZD-8055, Biological Replicate 2; Homo sapiens; RNA-Seq
Sample: SRS1326995
BioProject: PRJNA314507
BioSample: SAMN04536285
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2+/+ cells (number 15) treated with AZD-8055, RPF
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2+/+
treatment: AZD-8055
cell line id: 15
assayed molecule: Ribosome protected fragments
Original files (1)
TSC2+/+ cells (number 15) treated with AZD-8055, RPF
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208882 36256326 1849072626 814.52 15AZD_GCCAAT_L005_R1_001.fastq.gz, SRR3208882, SRR3208882.sralite SRA
SRX1617566 SRP071235 RNA-Seq SINGLE
GSM2082535: Total RNA of TSC2+/+ cells (number 15) treated with AZD-8055, Biological Replicate 1; Homo sapiens; RNA-Seq
Sample: SRS1326994
BioProject: PRJNA314507
BioSample: SAMN04536286
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2+/+ cells (number 15) treated with AZD-8055, total RNA
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2+/+
treatment: AZD-8055
cell line id: 15
assayed molecule: mRNA
Original files (1)
TSC2+/+ cells (number 15) treated with AZD-8055, total RNA
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208883 17966631 916298181 505.73 15AZD_ATCACG_L006_R1_001.fastq.gz, SRR3208883, SRR3208883.sralite SRA
SRX1617567 SRP071235 RNA-Seq SINGLE
GSM2082536: Total RNA of TSC2+/+ cells (number 15) treated with AZD-8055, Biological Replicate 2; Homo sapiens; RNA-Seq
Sample: SRS1326993
BioProject: PRJNA314507
BioSample: SAMN04536287
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2+/+ cells (number 15) treated with AZD-8055, total RNA
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2+/+
treatment: AZD-8055
cell line id: 15
assayed molecule: mRNA
Original files (1)
TSC2+/+ cells (number 15) treated with AZD-8055, total RNA
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208884 19703544 1004880744 464.55 15AZD_TGACCA_L008_R1_001.fastq.gz, SRR3208884, SRR3208884.sralite SRA
SRX1617568 SRP071235 RNA-Seq SINGLE
GSM2082537: Ribosome protected fragments (RPF) of TSC2+/+ cells (number 15) treated with DMSO, Biological Replicate 1; Homo sapiens; RNA-Seq
Sample: SRS1326992
BioProject: PRJNA314507
BioSample: SAMN04536302
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2+/+ cells (number 15) treated with DMSO, RPF
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2+/+
treatment: DMSO
cell line id: 15
assayed molecule: Ribosome protected fragments
Original files (1)
TSC2+/+ cells (number 15) treated with DMSO, RPF
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208885 29761657 1517844507 692.39 15DMSO_GCCAAT_L001_R1_001.fastq.gz, SRR3208885, SRR3208885.sralite SRA
SRX1617569 SRP071235 RNA-Seq SINGLE
GSM2082538: Ribosome protected fragments (RPF) of TSC2+/+ cells (number 15) treated with DMSO, Biological Replicate 2; Homo sapiens; RNA-Seq
Sample: SRS1326991
BioProject: PRJNA314507
BioSample: SAMN04536303
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2+/+ cells (number 15) treated with DMSO, RPF
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2+/+
treatment: DMSO
cell line id: 15
assayed molecule: Ribosome protected fragments
Original files (1)
TSC2+/+ cells (number 15) treated with DMSO, RPF
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208886 27716403 1413536553 822.94 15DMSO_ATCACG_L004_R1_001.fastq.gz, SRR3208886, SRR3208886.sralite SRA
SRX1617570 SRP071235 RNA-Seq SINGLE
GSM2082539: Total RNA of TSC2+/+ cells (number 15) treated with DMSO, Biological Replicate 1; Homo sapiens; RNA-Seq
Sample: SRS1326990
BioProject: PRJNA314507
BioSample: SAMN04536304
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2+/+ cells (number 15) treated with DMSO, total RNA
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2+/+
treatment: DMSO
cell line id: 15
assayed molecule: mRNA
Original files (1)
TSC2+/+ cells (number 15) treated with DMSO, total RNA
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208887 15775844 804568044 351.31 15DMSO_GCCAAT_L006_R1_001.fastq.gz, SRR3208887, SRR3208887.sralite SRA
SRX1617571 SRP071235 RNA-Seq SINGLE
GSM2082540: Total RNA of TSC2+/+ cells (number 15) treated with DMSO, Biological Replicate 2; Homo sapiens; RNA-Seq
Sample: SRS1326989
BioProject: PRJNA314507
BioSample: SAMN04536305
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2+/+ cells (number 15) treated with DMSO, total RNA
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2+/+
treatment: DMSO
cell line id: 15
assayed molecule: mRNA
Original files (1)
TSC2+/+ cells (number 15) treated with DMSO, total RNA
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208888 18549351 946016901 539.01 15DMSO_ATCACG_L007_R1_001.fastq.gz, SRR3208888, SRR3208888.sralite SRA
SRX1617572 SRP071235 RNA-Seq SINGLE
GSM2082541: Ribosome protected fragments (RPF) of TSC2+/+ cells (number 15) treated with Rapamycin, Biological Replicate 1; Homo sapiens; RNA-Seq
Sample: SRS1326988
BioProject: PRJNA314507
BioSample: SAMN04536306
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2+/+ cells (number 15) treated with Rapamycin, RPF
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2+/+
treatment: Rapamycin
cell line id: 15
assayed molecule: Ribosome protected fragments
Original files (1)
TSC2+/+ cells (number 15) treated with Rapamycin, RPF
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208889 37247298 1899612198 868.97 15Rapamycin_CTTGTA_L001_R1_001.fastq.gz, SRR3208889, SRR3208889.srali… SRA
SRX1617573 SRP071235 RNA-Seq SINGLE
GSM2082542: Ribosome protected fragments (RPF) of TSC2+/+ cells (number 15) treated with Rapamycin, Biological Replicate 2; Homo sapiens; RNA-Seq
Sample: SRS1326987
BioProject: PRJNA314507
BioSample: SAMN04536307
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2+/+ cells (number 15) treated with Rapamycin, RPF
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2+/+
treatment: Rapamycin
cell line id: 15
assayed molecule: Ribosome protected fragments
Original files (1)
TSC2+/+ cells (number 15) treated with Rapamycin, RPF
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208890 31257693 1594142343 697.22 15Rapamycin_CGATGT_L004_R1_001.fastq.gz, SRR3208890, SRR3208890.srali… SRA
SRX1617574 SRP071235 RNA-Seq SINGLE
GSM2082543: Total RNA of TSC2+/+ cells (number 15) treated with Rapamycin, Biological Replicate 1; Homo sapiens; RNA-Seq
Sample: SRS1326986
BioProject: PRJNA314507
BioSample: SAMN04536308
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2+/+ cells (number 15) treated with Rapamycin, total RNA
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2+/+
treatment: Rapamycin
cell line id: 15
assayed molecule: mRNA
Original files (1)
TSC2+/+ cells (number 15) treated with Rapamycin, total RNA
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208891 16211763 826799913 360.56 15Rapamycin_CTTGTA_L006_R1_001.fastq.gz, SRR3208891, SRR3208891.srali… SRA
SRX1617575 SRP071235 RNA-Seq SINGLE
GSM2082544: Total RNA of TSC2+/+ cells (number 15) treated with Rapamycin, Biological Replicate 2; Homo sapiens; RNA-Seq
Sample: SRS1326985
BioProject: PRJNA314507
BioSample: SAMN04536309
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2+/+ cells (number 15) treated with Rapamycin, total RNA
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2+/+
treatment: Rapamycin
cell line id: 15
assayed molecule: mRNA
Original files (1)
TSC2+/+ cells (number 15) treated with Rapamycin, total RNA
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208892 23189584 1182668784 526.29 15Rapamycin_CGATGT_L007_R1_001.fastq.gz, SRR3208892, SRR3208892.srali… SRA
SRX1617576 SRP071235 RNA-Seq SINGLE
GSM2082545: Ribosome protected fragments (RPF) of TSC2+/+ cells (number 16) treated with AZD-8055, Biological Replicate 1; Homo sapiens; RNA-Seq
Sample: SRS1326984
BioProject: PRJNA314507
BioSample: SAMN04536310
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2+/+ cells (number 16) treated with AZD-8055, RPF
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2+/+
treatment: AZD-8055
cell line id: 16
assayed molecule: Ribosome protected fragments
Original files (1)
TSC2+/+ cells (number 16) treated with AZD-8055, RPF
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208893 28201827 1438293177 630.45 16AZD_TGACCA_L002_R1_001.fastq.gz, SRR3208893, SRR3208893.sralite SRA
SRX1617577 SRP071235 RNA-Seq SINGLE
GSM2082546: Ribosome protected fragments (RPF) of TSC2+/+ cells (number 16) treated with AZD-8055, Biological Replicate 2; Homo sapiens; RNA-Seq
Sample: SRS1326983
BioProject: PRJNA314507
BioSample: SAMN04536311
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2+/+ cells (number 16) treated with AZD-8055, RPF
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2+/+
treatment: AZD-8055
cell line id: 16
assayed molecule: Ribosome protected fragments
Original files (1)
TSC2+/+ cells (number 16) treated with AZD-8055, RPF
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208894 43855305 2236620555 986.49 16AZD_TGACCA_L005_R1_001.fastq.gz, SRR3208894, SRR3208894.sralite SRA
SRX1617578 SRP071235 RNA-Seq SINGLE
GSM2082547: Total RNA of TSC2+/+ cells (number 16) treated with AZD-8055, Biological Replicate 1; Homo sapiens; RNA-Seq
Sample: SRS1326982
BioProject: PRJNA314507
BioSample: SAMN04536312
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2+/+ cells (number 16) treated with AZD-8055, total RNA
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2+/+
treatment: AZD-8055
cell line id: 16
assayed molecule: mRNA
Original files (1)
TSC2+/+ cells (number 16) treated with AZD-8055, total RNA
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208895 17309785 882799035 393.33 16AZD_ACTTGA_L007_R1_001.fastq.gz, SRR3208895, SRR3208895.sralite SRA
SRX1617579 SRP071235 RNA-Seq SINGLE
GSM2082548: Total RNA of TSC2+/+ cells (number 16) treated with AZD-8055, Biological Replicate 2; Homo sapiens; RNA-Seq
Sample: SRS1326981
BioProject: PRJNA314507
BioSample: SAMN04536313
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2+/+ cells (number 16) treated with AZD-8055, total RNA
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2+/+
treatment: AZD-8055
cell line id: 16
assayed molecule: mRNA
Original files (1)
TSC2+/+ cells (number 16) treated with AZD-8055, total RNA
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208896 17708360 903126360 416.88 16AZD_ACTTGA_L008_R1_001.fastq.gz, SRR3208896, SRR3208896.sralite SRA
SRX1617580 SRP071235 RNA-Seq SINGLE
GSM2082549: Ribosome protected fragments (RPF) of TSC2+/+ cells (number 16) treated with DMSO, Biological Replicate 1; Homo sapiens; RNA-Seq
Sample: SRS1326980
BioProject: PRJNA314507
BioSample: SAMN04536314
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2+/+ cells (number 16) treated with DMSO, RPF
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2+/+
treatment: DMSO
cell line id: 16
assayed molecule: Ribosome protected fragments
Original files (1)
TSC2+/+ cells (number 16) treated with DMSO, RPF
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208897 30995772 1580784372 689.1 16DMSO_GCCAAT_L002_R1_001.fastq.gz, SRR3208897, SRR3208897.sralite SRA
SRX1617581 SRP071235 RNA-Seq SINGLE
GSM2082550: Ribosome protected fragments (RPF) of TSC2+/+ cells (number 16) treated with DMSO, Biological Replicate 2; Homo sapiens; RNA-Seq
Sample: SRS1326979
BioProject: PRJNA314507
BioSample: SAMN04536315
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2+/+ cells (number 16) treated with DMSO, RPF
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2+/+
treatment: DMSO
cell line id: 16
assayed molecule: Ribosome protected fragments
Original files (1)
TSC2+/+ cells (number 16) treated with DMSO, RPF
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208898 39485961 2013784011 887.18 16DMSO_CTTGTA_L005_R1_001.fastq.gz, SRR3208898, SRR3208898.sralite SRA
SRX1617582 SRP071235 RNA-Seq SINGLE
GSM2082551: Total RNA of TSC2+/+ cells (number 16) treated with DMSO, Biological Replicate 1; Homo sapiens; RNA-Seq
Sample: SRS1326978
BioProject: PRJNA314507
BioSample: SAMN04536316
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2+/+ cells (number 16) treated with DMSO, total RNA
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2+/+
treatment: DMSO
cell line id: 16
assayed molecule: mRNA
Original files (1)
TSC2+/+ cells (number 16) treated with DMSO, total RNA
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208899 18809039 959260989 426.7 16DMSO_GCCAAT_L007_R1_001.fastq.gz, SRR3208899, SRR3208899.sralite SRA
SRX1617583 SRP071235 RNA-Seq SINGLE
GSM2082552: Total RNA of TSC2+/+ cells (number 16) treated with DMSO, Biological Replicate 2; Homo sapiens; RNA-Seq
Sample: SRS1326977
BioProject: PRJNA314507
BioSample: SAMN04536317
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2+/+ cells (number 16) treated with DMSO, total RNA
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2+/+
treatment: DMSO
cell line id: 16
assayed molecule: mRNA
Original files (1)
TSC2+/+ cells (number 16) treated with DMSO, total RNA
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208900 25327906 1291723206 593.8 16DMSO_ACAGTG_L008_R1_001.fastq.gz, SRR3208900, SRR3208900.sralite SRA
SRX1617584 SRP071235 RNA-Seq SINGLE
GSM2082553: Ribosome protected fragments (RPF) of TSC2+/+ cells (number 16) treated with Rapamycin, Biological Replicate 1; Homo sapiens; RNA-Seq
Sample: SRS1326976
BioProject: PRJNA314507
BioSample: SAMN04536318
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2+/+ cells (number 16) treated with Rapamycin, RPF
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2+/+
treatment: Rapamycin
cell line id: 16
assayed molecule: Ribosome protected fragments
Original files (1)
TSC2+/+ cells (number 16) treated with Rapamycin, RPF
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208901 29169219 1487630169 651.38 16Rapamycin_CTTGTA_L002_R1_001.fastq.gz, SRR3208901, SRR3208901.srali… SRA
SRX1617585 SRP071235 RNA-Seq SINGLE
GSM2082554: Ribosome protected fragments (RPF) of TSC2+/+ cells (number 16) treated with Rapamycin, Biological Replicate 2; Homo sapiens; RNA-Seq
Sample: SRS1326975
BioProject: PRJNA314507
BioSample: SAMN04536319
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2+/+ cells (number 16) treated with Rapamycin, RPF
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2+/+
treatment: Rapamycin
cell line id: 16
assayed molecule: Ribosome protected fragments
Original files (1)
TSC2+/+ cells (number 16) treated with Rapamycin, RPF
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208902 45182434 2304304134 1010.02 16Rapamycin_TTAGGC_L005_R1_001.fastq.gz, SRR3208902, SRR3208902.srali… SRA
SRX1617586 SRP071235 RNA-Seq SINGLE
GSM2082555: Total RNA of TSC2+/+ cells (number 16) treated with Rapamycin, Biological Replicate 1; Homo sapiens; RNA-Seq
Sample: SRS1326974
BioProject: PRJNA314507
BioSample: SAMN04536320
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2+/+ cells (number 16) treated with Rapamycin, total RNA
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2+/+
treatment: Rapamycin
cell line id: 16
assayed molecule: mRNA
Original files (1)
TSC2+/+ cells (number 16) treated with Rapamycin, total RNA
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208903 14947079 762301029 339.59 16Rapamycin_CTTGTA_L007_R1_001.fastq.gz, SRR3208903, SRR3208903.srali… SRA
SRX1617587 SRP071235 RNA-Seq SINGLE
GSM2082556: Total RNA of TSC2+/+ cells (number 16) treated with Rapamycin, Biological Replicate 2; Homo sapiens; RNA-Seq
Sample: SRS1326973
BioProject: PRJNA314507
BioSample: SAMN04536321
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2+/+ cells (number 16) treated with Rapamycin, total RNA
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2+/+
treatment: Rapamycin
cell line id: 16
assayed molecule: mRNA
Original files (1)
TSC2+/+ cells (number 16) treated with Rapamycin, total RNA
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208904 18066608 921397008 425.47 16Rapamycin_CAGATC_L008_R1_001.fastq.gz, SRR3208904, SRR3208904.srali… SRA
SRX1617588 SRP071235 RNA-Seq SINGLE
GSM2082557: Ribosome protected fragments (RPF) of TSC2-/- cells (number 19) treated with AZD-8055, Biological Replicate 1; Homo sapiens; RNA-Seq
Sample: SRS1326972
BioProject: PRJNA314507
BioSample: SAMN04536322
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2-/- cells (number 19) treated with AZD-8055, RPF
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2-/-
treatment: AZD-8055
cell line id: 19
assayed molecule: Ribosome protected fragments
Original files (1)
TSC2-/- cells (number 19) treated with AZD-8055, RPF
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208905 23241417 1185312267 519.5 19AZD_ACAGTG_L002_R1_001.fastq.gz, SRR3208905, SRR3208905.sralite SRA
SRX1617589 SRP071235 RNA-Seq SINGLE
GSM2082558: Ribosome protected fragments (RPF) of TSC2-/- cells (number 19) treated with AZD-8055, Biological Replicate 2; Homo sapiens; RNA-Seq
Sample: SRS1326971
BioProject: PRJNA314507
BioSample: SAMN04536323
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2-/- cells (number 19) treated with AZD-8055, RPF
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2-/-
treatment: AZD-8055
cell line id: 19
assayed molecule: Ribosome protected fragments
Original files (1)
TSC2-/- cells (number 19) treated with AZD-8055, RPF
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208906 32511884 1658106084 730.66 19AZD_ACTTGA_L003_R1_001.fastq.gz, SRR3208906, SRR3208906.sralite SRA
SRX1617590 SRP071235 RNA-Seq SINGLE
GSM2082559: Total RNA of TSC2-/- cells (number 19) treated with AZD-8055, Biological Replicate 1; Homo sapiens; RNA-Seq
Sample: SRS1326970
BioProject: PRJNA314507
BioSample: SAMN04536324
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2-/- cells (number 19) treated with AZD-8055, total RNA
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2-/-
treatment: AZD-8055
cell line id: 19
assayed molecule: mRNA
Original files (1)
TSC2-/- cells (number 19) treated with AZD-8055, total RNA
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208907 18777105 957632355 416.83 19AZD_TGACCA_L006_R1_001.fastq.gz, SRR3208907, SRR3208907.sralite SRA
SRX1617591 SRP071235 RNA-Seq SINGLE
GSM2082560: Total RNA of TSC2-/- cells (number 19) treated with AZD-8055, Biological Replicate 2; Homo sapiens; RNA-Seq
Sample: SRS1326969
BioProject: PRJNA314507
BioSample: SAMN04536325
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2-/- cells (number 19) treated with AZD-8055, total RNA
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2-/-
treatment: AZD-8055
cell line id: 19
assayed molecule: mRNA
Original files (1)
TSC2-/- cells (number 19) treated with AZD-8055, total RNA
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208908 20073766 1023762066 473.32 19AZD_TTAGGC_L008_R1_001.fastq.gz, SRR3208908, SRR3208908.sralite SRA
SRX1617592 SRP071235 RNA-Seq SINGLE
GSM2082561: Ribosome protected fragments (RPF) of TSC2-/- cells (number 19) treated with DMSO, Biological Replicate 1; Homo sapiens; RNA-Seq
Sample: SRS1326968
BioProject: PRJNA314507
BioSample: SAMN04536326
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2-/- cells (number 19) treated with DMSO, RPF
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2-/-
treatment: DMSO
cell line id: 19
assayed molecule: Ribosome protected fragments
Original files (1)
TSC2-/- cells (number 19) treated with DMSO, RPF
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208909 34444133 1756650783 799.47 19DMSO_CGATGT_L001_R1_001.fastq.gz, SRR3208909, SRR3208909.sralite SRA
SRX1617593 SRP071235 RNA-Seq SINGLE
GSM2082562: Ribosome protected fragments (RPF) of TSC2-/- cells (number 19) treated with DMSO, Biological Replicate 2; Homo sapiens; RNA-Seq
Sample: SRS1326967
BioProject: PRJNA314507
BioSample: SAMN04536327
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2-/- cells (number 19) treated with DMSO, RPF
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2-/-
treatment: DMSO
cell line id: 19
assayed molecule: Ribosome protected fragments
Original files (1)
TSC2-/- cells (number 19) treated with DMSO, RPF
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208910 29011354 1479579054 652.83 19DMSO_GCCAAT_L003_R1_001.fastq.gz, SRR3208910, SRR3208910.sralite SRA
SRX1617594 SRP071235 RNA-Seq SINGLE
GSM2082563: Total RNA of TSC2-/- cells (number 19) treated with DMSO, Biological Replicate 1; Homo sapiens; RNA-Seq
Sample: SRS1326966
BioProject: PRJNA314507
BioSample: SAMN04536328
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2-/- cells (number 19) treated with DMSO, total RNA
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2-/-
treatment: DMSO
cell line id: 19
assayed molecule: mRNA
Original files (1)
TSC2-/- cells (number 19) treated with DMSO, total RNA
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208911 18456779 941295729 408.4 19DMSO_CGATGT_L006_R1_001.fastq.gz, SRR3208911, SRR3208911.sralite SRA
SRX1617595 SRP071235 RNA-Seq SINGLE
GSM2082564: Total RNA of TSC2-/- cells (number 19) treated with DMSO, Biological Replicate 2; Homo sapiens; RNA-Seq
Sample: SRS1326965
BioProject: PRJNA314507
BioSample: SAMN04536329
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2-/- cells (number 19) treated with DMSO, total RNA
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2-/-
treatment: DMSO
cell line id: 19
assayed molecule: mRNA
Original files (1)
TSC2-/- cells (number 19) treated with DMSO, total RNA
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208912 18808752 959246352 443.15 19DMSO_GCCAAT_L008_R1_001.fastq.gz, SRR3208912, SRR3208912.sralite SRA
SRX1617596 SRP071235 RNA-Seq SINGLE
GSM2082565: Ribosome protected fragments (RPF) of TSC2-/- cells (number 19) treated with Rapamycin, Biological Replicate 1; Homo sapiens; RNA-Seq
Sample: SRS1326964
BioProject: PRJNA314507
BioSample: SAMN04536330
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2-/- cells (number 19) treated with Rapamycin, RPF
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2-/-
treatment: Rapamycin
cell line id: 19
assayed molecule: Ribosome protected fragments
Original files (1)
TSC2-/- cells (number 19) treated with Rapamycin, RPF
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208913 48288363 2462706513 1117.68 19Rapamycin_TTAGGC_L001_R1_001.fastq.gz, SRR3208913, SRR3208913.srali… SRA
SRX1617597 SRP071235 RNA-Seq SINGLE
GSM2082566: Ribosome protected fragments (RPF) of TSC2-/- cells (number 19) treated with Rapamycin, Biological Replicate 2; Homo sapiens; RNA-Seq
Sample: SRS1326963
BioProject: PRJNA314507
BioSample: SAMN04536331
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2-/- cells (number 19) treated with Rapamycin, RPF
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2-/-
treatment: Rapamycin
cell line id: 19
assayed molecule: Ribosome protected fragments
Original files (1)
TSC2-/- cells (number 19) treated with Rapamycin, RPF
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208914 27233282 1388897382 616.51 19Rapamycin_CTTGTA_L003_R1_001.fastq.gz, SRR3208914, SRR3208914.srali… SRA
SRX1617598 SRP071235 RNA-Seq SINGLE
GSM2082567: Total RNA of TSC2-/- cells (number 19) treated with Rapamycin, Biological Replicate 1; Homo sapiens; RNA-Seq
Sample: SRS1326949
BioProject: PRJNA314507
BioSample: SAMN04536288
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2-/- cells (number 19) treated with Rapamycin, total RNA
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2-/-
treatment: Rapamycin
cell line id: 19
assayed molecule: mRNA
Original files (1)
TSC2-/- cells (number 19) treated with Rapamycin, total RNA
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208915 18775607 957555957 417.41 19Rapamycin_TTAGGC_L006_R1_001.fastq.gz, SRR3208915, SRR3208915.srali… SRA
SRX1617599 SRP071235 RNA-Seq SINGLE
GSM2082568: Total RNA of TSC2-/- cells (number 19) treated with Rapamycin, Biological Replicate 2; Homo sapiens; RNA-Seq
Sample: SRS1326962
BioProject: PRJNA314507
BioSample: SAMN04536289
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2-/- cells (number 19) treated with Rapamycin, total RNA
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2-/-
treatment: Rapamycin
cell line id: 19
assayed molecule: mRNA
Original files (1)
TSC2-/- cells (number 19) treated with Rapamycin, total RNA
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208916 18710575 954239325 442.94 19Rapamycin_CTTGTA_L008_R1_001.fastq.gz, SRR3208916, SRR3208916.srali… SRA
SRX1617600 SRP071235 RNA-Seq SINGLE
GSM2082569: Ribosome protected fragments (RPF) of TSC2-/- cells (number 20) treated with AZD-8055, Biological Replicate 1; Homo sapiens; RNA-Seq
Sample: SRS1326961
BioProject: PRJNA314507
BioSample: SAMN04536290
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2-/- cells (number 20) treated with AZD-8055, RPF
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2-/-
treatment: AZD-8055
cell line id: 20
assayed molecule: Ribosome protected fragments
Original files (1)
TSC2-/- cells (number 20) treated with AZD-8055, RPF
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208917 28802328 1468918728 649.84 20AZD_TAGCTT_L003_R1_001.fastq.gz, SRR3208917, SRR3208917.sralite SRA
SRX1617601 SRP071235 RNA-Seq SINGLE
GSM2082570: Ribosome protected fragments (RPF) of TSC2-/- cells (number 20) treated with AZD-8055, Biological Replicate 2; Homo sapiens; RNA-Seq
Sample: SRS1326960
BioProject: PRJNA314507
BioSample: SAMN04536291
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2-/- cells (number 20) treated with AZD-8055, RPF
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2-/-
treatment: AZD-8055
cell line id: 20
assayed molecule: Ribosome protected fragments
Original files (1)
TSC2-/- cells (number 20) treated with AZD-8055, RPF
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208918 32924832 1679166432 735.49 20AZD_GGCTAC_L004_R1_001.fastq.gz, SRR3208918, SRR3208918.sralite SRA
SRX1617602 SRP071235 RNA-Seq SINGLE
GSM2082571: Total RNA of TSC2-/- cells (number 20) treated with AZD-8055, Biological Replicate 1; Homo sapiens; RNA-Seq
Sample: SRS1326959
BioProject: PRJNA314507
BioSample: SAMN04536292
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2-/- cells (number 20) treated with AZD-8055, total RNA
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2-/-
treatment: AZD-8055
cell line id: 20
assayed molecule: mRNA
Original files (1)
TSC2-/- cells (number 20) treated with AZD-8055, total RNA
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208919 17683561 901861611 403.33 20AZD_GGCTAC_L007_R1_001.fastq.gz, SRR3208919, SRR3208919.sralite SRA
SRX1617603 SRP071235 RNA-Seq SINGLE
GSM2082572: Total RNA of TSC2-/- cells (number 20) treated with AZD-8055, Biological Replicate 2; Homo sapiens; RNA-Seq
Sample: SRS1326958
BioProject: PRJNA314507
BioSample: SAMN04536293
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2-/- cells (number 20) treated with AZD-8055, total RNA
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2-/-
treatment: AZD-8055
cell line id: 20
assayed molecule: mRNA
Original files (1)
TSC2-/- cells (number 20) treated with AZD-8055, total RNA
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208920 17831122 909387222 419.92 20AZD_GATCAG_L008_R1_001.fastq.gz, SRR3208920, SRR3208920.sralite SRA
SRX1617604 SRP071235 RNA-Seq SINGLE
GSM2082573: Ribosome protected fragments (RPF) of TSC2-/- cells (number 20) treated with DMSO, Biological Replicate 1; Homo sapiens; RNA-Seq
Sample: SRS1326957
BioProject: PRJNA314507
BioSample: SAMN04536294
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2-/- cells (number 20) treated with DMSO, RPF
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2-/-
treatment: DMSO
cell line id: 20
assayed molecule: Ribosome protected fragments
Original files (1)
TSC2-/- cells (number 20) treated with DMSO, RPF
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208921 31613896 1612308696 702.76 20DMSO_CAGATC_L002_R1_001.fastq.gz, SRR3208921, SRR3208921.sralite SRA
SRX1617605 SRP071235 RNA-Seq SINGLE
GSM2082574: Ribosome protected fragments (RPF) of TSC2-/- cells (number 20) treated with DMSO, Biological Replicate 2; Homo sapiens; RNA-Seq
Sample: SRS1326956
BioProject: PRJNA314507
BioSample: SAMN04536295
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2-/- cells (number 20) treated with DMSO, RPF
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2-/-
treatment: DMSO
cell line id: 20
assayed molecule: Ribosome protected fragments
Original files (1)
TSC2-/- cells (number 20) treated with DMSO, RPF
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208922 28008414 1428429114 619.48 20DMSO_GCCAAT_L004_R1_001.fastq.gz, SRR3208922, SRR3208922.sralite SRA
SRX1617606 SRP071235 RNA-Seq SINGLE
GSM2082575: Total RNA of TSC2-/- cells (number 20) treated with DMSO, Biological Replicate 1; Homo sapiens; RNA-Seq
Sample: SRS1326955
BioProject: PRJNA314507
BioSample: SAMN04536296
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2-/- cells (number 20) treated with DMSO, total RNA
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2-/-
treatment: DMSO
cell line id: 20
assayed molecule: mRNA
Original files (1)
TSC2-/- cells (number 20) treated with DMSO, total RNA
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208923 19818415 1010739165 439.2 20DMSO_ACAGTG_L006_R1_001.fastq.gz, SRR3208923, SRR3208923.sralite SRA
SRX1617607 SRP071235 RNA-Seq SINGLE
GSM2082576: Total RNA of TSC2-/- cells (number 20) treated with DMSO, Biological Replicate 2; Homo sapiens; RNA-Seq
Sample: SRS1326954
BioProject: PRJNA314507
BioSample: SAMN04536297
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2-/- cells (number 20) treated with DMSO, total RNA
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2-/-
treatment: DMSO
cell line id: 20
assayed molecule: mRNA
Original files (1)
TSC2-/- cells (number 20) treated with DMSO, total RNA
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208924 18556983 946406133 421.44 20DMSO_GATCAG_L007_R1_001.fastq.gz, SRR3208924, SRR3208924.sralite SRA
SRX1617608 SRP071235 RNA-Seq SINGLE
GSM2082577: Ribosome protected fragments (RPF) of TSC2-/- cells (number 20) treated with Rapamycin, Biological Replicate 1; Homo sapiens; RNA-Seq
Sample: SRS1326953
BioProject: PRJNA314507
BioSample: SAMN04536298
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2-/- cells (number 20) treated with Rapamycin, RPF
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2-/-
treatment: Rapamycin
cell line id: 20
assayed molecule: Ribosome protected fragments
Original files (1)
TSC2-/- cells (number 20) treated with Rapamycin, RPF
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208925 33823474 1724997174 762.41 20Rapamycin_GATCAG_L003_R1_001.fastq.gz, SRR3208925, SRR3208925.srali… SRA
SRX1617609 SRP071235 RNA-Seq SINGLE
GSM2082578: Ribosome protected fragments (RPF) of TSC2-/- cells (number 20) treated with Rapamycin, Biological Replicate 2; Homo sapiens; RNA-Seq
Sample: SRS1326952
BioProject: PRJNA314507
BioSample: SAMN04536299
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2-/- cells (number 20) treated with Rapamycin, RPF
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2-/-
treatment: Rapamycin
cell line id: 20
assayed molecule: Ribosome protected fragments
Original files (1)
TSC2-/- cells (number 20) treated with Rapamycin, RPF
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208926 28958327 1476874677 643.19 20Rapamycin_CTTGTA_L004_R1_001.fastq.gz, SRR3208926, SRR3208926.srali… SRA
SRX1617610 SRP071235 RNA-Seq SINGLE
GSM2082579: Total RNA of TSC2-/- cells (number 20) treated with Rapamycin, Biological Replicate 1; Homo sapiens; RNA-Seq
Sample: SRS1326951
BioProject: PRJNA314507
BioSample: SAMN04536300
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2-/- cells (number 20) treated with Rapamycin, total RNA
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2-/-
treatment: Rapamycin
cell line id: 20
assayed molecule: mRNA
Original files (1)
TSC2-/- cells (number 20) treated with Rapamycin, total RNA
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208927 17717903 903613053 393.3 20Rapamycin_CAGATC_L006_R1_001.fastq.gz, SRR3208927, SRR3208927.srali… SRA
SRX1617611 SRP071235 RNA-Seq SINGLE
GSM2082580: Total RNA of TSC2-/- cells (number 20) treated with Rapamycin, Biological Replicate 2; Homo sapiens; RNA-Seq
Sample: SRS1326950
BioProject: PRJNA314507
BioSample: SAMN04536301
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: TSC2-/- cells (number 20) treated with Rapamycin, total RNA
cell type: Embryonic stem cell-derived neurons
genotype/variation: TSC2-/-
treatment: Rapamycin
cell line id: 20
assayed molecule: mRNA
Original files (1)
TSC2-/- cells (number 20) treated with Rapamycin, total RNA
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3208928 18330910 934876410 416.03 20Rapamycin_TAGCTT_L007_R1_001.fastq.gz, SRR3208928, SRR3208928.srali… SRA

Linked Publications (1)

Genomic analysis of the molecular neuropathology of tuberous sclerosis using a human stem cell model.
Genome medicine · 2016

Data Files (96)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
— 15AZD_ATCACG_L001_R1_001.fastq.gz RNA-Seq 831.4 MB link
— 15AZD_ATCACG_L006_R1_001.fastq.gz RNA-Seq 505.7 MB link
— 15AZD_GCCAAT_L005_R1_001.fastq.gz RNA-Seq 814.5 MB link
— 15AZD_TGACCA_L008_R1_001.fastq.gz RNA-Seq 464.5 MB link
— 15DMSO_ATCACG_L004_R1_001.fastq.gz RNA-Seq 822.9 MB link
— 15DMSO_ATCACG_L007_R1_001.fastq.gz RNA-Seq 539.0 MB link
— 15DMSO_GCCAAT_L001_R1_001.fastq.gz RNA-Seq 692.4 MB link
— 15DMSO_GCCAAT_L006_R1_001.fastq.gz RNA-Seq 351.3 MB link
— 15Rapamycin_CGATGT_L004_R1_001.fastq.gz RNA-Seq 697.2 MB link
— 15Rapamycin_CGATGT_L007_R1_001.fastq.gz RNA-Seq 526.3 MB link
— 15Rapamycin_CTTGTA_L001_R1_001.fastq.gz RNA-Seq 869.0 MB link
— 15Rapamycin_CTTGTA_L006_R1_001.fastq.gz RNA-Seq 360.6 MB link
— 16AZD_ACTTGA_L007_R1_001.fastq.gz RNA-Seq 393.3 MB link
— 16AZD_ACTTGA_L008_R1_001.fastq.gz RNA-Seq 416.9 MB link
— 16AZD_TGACCA_L002_R1_001.fastq.gz RNA-Seq 630.4 MB link
— 16AZD_TGACCA_L005_R1_001.fastq.gz RNA-Seq 986.5 MB link
— 16DMSO_ACAGTG_L008_R1_001.fastq.gz RNA-Seq 593.8 MB link
— 16DMSO_CTTGTA_L005_R1_001.fastq.gz RNA-Seq 887.2 MB link
— 16DMSO_GCCAAT_L002_R1_001.fastq.gz RNA-Seq 689.1 MB link
— 16DMSO_GCCAAT_L007_R1_001.fastq.gz RNA-Seq 426.7 MB link
— 16Rapamycin_CAGATC_L008_R1_001.fastq.gz RNA-Seq 425.5 MB link
— 16Rapamycin_CTTGTA_L002_R1_001.fastq.gz RNA-Seq 651.4 MB link
— 16Rapamycin_CTTGTA_L007_R1_001.fastq.gz RNA-Seq 339.6 MB link
— 16Rapamycin_TTAGGC_L005_R1_001.fastq.gz RNA-Seq 1010.0 MB link
— 19AZD_ACAGTG_L002_R1_001.fastq.gz RNA-Seq 519.5 MB link
— 19AZD_ACTTGA_L003_R1_001.fastq.gz RNA-Seq 730.7 MB link
— 19AZD_TGACCA_L006_R1_001.fastq.gz RNA-Seq 416.8 MB link
— 19AZD_TTAGGC_L008_R1_001.fastq.gz RNA-Seq 473.3 MB link
— 19DMSO_CGATGT_L001_R1_001.fastq.gz RNA-Seq 799.5 MB link
— 19DMSO_CGATGT_L006_R1_001.fastq.gz RNA-Seq 408.4 MB link
— 19DMSO_GCCAAT_L003_R1_001.fastq.gz RNA-Seq 652.8 MB link
— 19DMSO_GCCAAT_L008_R1_001.fastq.gz RNA-Seq 443.1 MB link
— 19Rapamycin_CTTGTA_L003_R1_001.fastq.gz RNA-Seq 616.5 MB link
— 19Rapamycin_CTTGTA_L008_R1_001.fastq.gz RNA-Seq 442.9 MB link
— 19Rapamycin_TTAGGC_L001_R1_001.fastq.gz RNA-Seq 1.1 GB link
— 19Rapamycin_TTAGGC_L006_R1_001.fastq.gz RNA-Seq 417.4 MB link
— 20AZD_GATCAG_L008_R1_001.fastq.gz RNA-Seq 419.9 MB link
— 20AZD_GGCTAC_L004_R1_001.fastq.gz RNA-Seq 735.5 MB link
— 20AZD_GGCTAC_L007_R1_001.fastq.gz RNA-Seq 403.3 MB link
— 20AZD_TAGCTT_L003_R1_001.fastq.gz RNA-Seq 649.8 MB link
— 20DMSO_ACAGTG_L006_R1_001.fastq.gz RNA-Seq 439.2 MB link
— 20DMSO_CAGATC_L002_R1_001.fastq.gz RNA-Seq 702.8 MB link
— 20DMSO_GATCAG_L007_R1_001.fastq.gz RNA-Seq 421.4 MB link
— 20DMSO_GCCAAT_L004_R1_001.fastq.gz RNA-Seq 619.5 MB link
— 20Rapamycin_CAGATC_L006_R1_001.fastq.gz RNA-Seq 393.3 MB link
— 20Rapamycin_CTTGTA_L004_R1_001.fastq.gz RNA-Seq 643.2 MB link
— 20Rapamycin_GATCAG_L003_R1_001.fastq.gz RNA-Seq 762.4 MB link
— 20Rapamycin_TAGCTT_L007_R1_001.fastq.gz RNA-Seq 416.0 MB link
— SRR3208881_GSM2082533 RNA-Seq 831.0 MB link
— SRR3208882_GSM2082534 RNA-Seq 814.0 MB link
— SRR3208883_GSM2082535 RNA-Seq 505.0 MB link
— SRR3208884_GSM2082536 RNA-Seq 464.0 MB link
— SRR3208885_GSM2082537 RNA-Seq 692.0 MB link
— SRR3208886_GSM2082538 RNA-Seq 822.0 MB link
— SRR3208887_GSM2082539 RNA-Seq 351.0 MB link
— SRR3208888_GSM2082540 RNA-Seq 539.0 MB link
— SRR3208889_GSM2082541 RNA-Seq 868.0 MB link
— SRR3208890_GSM2082542 RNA-Seq 697.0 MB link
— SRR3208891_GSM2082543 RNA-Seq 360.0 MB link
— SRR3208892_GSM2082544 RNA-Seq 526.0 MB link
— SRR3208893_GSM2082545 RNA-Seq 630.0 MB link
— SRR3208894_GSM2082546 RNA-Seq 986.0 MB link
— SRR3208895_GSM2082547 RNA-Seq 393.0 MB link
— SRR3208896_GSM2082548 RNA-Seq 416.0 MB link
— SRR3208897_GSM2082549 RNA-Seq 689.0 MB link
— SRR3208898_GSM2082550 RNA-Seq 887.0 MB link
— SRR3208899_GSM2082551 RNA-Seq 426.0 MB link
— SRR3208900_GSM2082552 RNA-Seq 593.0 MB link
— SRR3208901_GSM2082553 RNA-Seq 651.0 MB link
— SRR3208902_GSM2082554 RNA-Seq 1010.0 MB link
— SRR3208903_GSM2082555 RNA-Seq 339.0 MB link
— SRR3208904_GSM2082556 RNA-Seq 425.0 MB link
— SRR3208905_GSM2082557 RNA-Seq 519.0 MB link
— SRR3208906_GSM2082558 RNA-Seq 730.0 MB link
— SRR3208907_GSM2082559 RNA-Seq 416.0 MB link
— SRR3208908_GSM2082560 RNA-Seq 473.0 MB link
— SRR3208909_GSM2082561 RNA-Seq 799.0 MB link
— SRR3208910_GSM2082562 RNA-Seq 652.0 MB link
— SRR3208911_GSM2082563 RNA-Seq 408.0 MB link
— SRR3208912_GSM2082564 RNA-Seq 443.0 MB link
— SRR3208913_GSM2082565 RNA-Seq 1.1 GB link
— SRR3208914_GSM2082566 RNA-Seq 616.0 MB link
— SRR3208915_GSM2082567 RNA-Seq 417.0 MB link
— SRR3208916_GSM2082568 RNA-Seq 442.0 MB link
— SRR3208917_GSM2082569 RNA-Seq 649.0 MB link
— SRR3208918_GSM2082570 RNA-Seq 735.0 MB link
— SRR3208919_GSM2082571 RNA-Seq 403.0 MB link
— SRR3208920_GSM2082572 RNA-Seq 419.0 MB link
— SRR3208921_GSM2082573 RNA-Seq 702.0 MB link
— SRR3208922_GSM2082574 RNA-Seq 619.0 MB link
— SRR3208923_GSM2082575 RNA-Seq 439.0 MB link
— SRR3208924_GSM2082576 RNA-Seq 421.0 MB link
— SRR3208925_GSM2082577 RNA-Seq 762.0 MB link
— SRR3208926_GSM2082578 RNA-Seq 643.0 MB link
— SRR3208927_GSM2082579 RNA-Seq 393.0 MB link
— SRR3208928_GSM2082580 RNA-Seq 416.0 MB link