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GSE77629

GSE GEO
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Enhanced CLIP (eCLIP) enables robust and scalable transcriptome-wide discovery and characterization of RNA binding protein binding sites [eCLIP - 293T cells]

Organism: Homo sapiens
Platform: GPL16791
Samples: 24
Experiment Types:
Other
Submitted: Feb 05 2016
Last Updated: May 15 2019
Status: Public on Mar 28 2016
Contact: Gene,,Yeo (UCSD)

Relations

SubSeries of: GSE77634 BioProject: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA311053 SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRP069351

Summary

RNA binding proteins (RBPs) play essential roles in cellular physiology by interacting with target RNAs. As defects in protein-RNA recognition lead to human disease, UV-crosslinking and immunoprecipitation (CLIP) of ribonuclear complexes followed by deep sequencing (-seq) is critical in constructing protein-RNA maps to expand our understanding of RBP function. However, current CLIP protocols are technically demanding and involve low complexity libraries that yield squandered sequencing of PCR duplicates and high experimental failure rates. To enable truly large-scale implementation of CLIP-seq, we have developed an enhanced CLIP methodology (eCLIP) that features a decrease of ~10 cycles of requisite amplification with a concomitant >60% decrease in discarded PCR duplicate reads, while maintaining the ability to identify RNA binding with single-nucleotide resolution. By simplifying the generation of paired IgG and size-matched input controls, eCLIP also dramatically improves specificity in discovery of authentic binding sites. To demonstrate that eCLIP enables large-scale and robust profiling of RBPs, 102 eCLIP experiments in biological duplicate for a diverse collection of 74 RBPs in HepG2 and K562 cells were completed (available at https://www.encodeproject.org). We establish that eCLIP is comparable in amplification and sample requirements to ChIP-seq, and enables integrative analysis of diverse RBPs to reveal factor-specific profiles, common artifacts for CLIP experiments and RNA-centric perspectives of RBP activity.

Overall Design

eCLIP-seq against RBFOX2 and SLBP in 293T Cells

Analysis (32 steps)

View Data Processing
Processing steps for GSE77629
  1. Takes output from raw files.
  2. Run to trim off both 5’ and 3’ adapters on both reads.
  3. Command: quality-cutoff 6 -m 18 -a NNNNNAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -g CTTCCGATCTACAAGTT -g CTTCCGATCTTGGTCCT -A AACTTGTAGATCGGA -A AGGACCAAGATCGGA -A ACTTGTAGATCGGAA -A GGACCAAGATCGGAA -A CTTGT AGATCGGAAG -A GACCAAGATCGGAAG -A TTGTAGATCGGAAGA -A ACCAAGATCGGAAGA -A TGTAGATCGGAAGAG -A CCAAGATCGGAAGAG -A GTAGATCGGAAGAGC -A CAAGATCGGAAGAGC -A TAGATCGGAAGAGCG -A AAGATCGGAAGAGCG -A AGATCGGAAGAGCGT -A GATCGGAAGAGCGTC -A ATCGGAAGAGCGTCG -A TCGGAAGAGCGTCGT -A CGGAAGAGCGTCGTG -A GGAAGAGCGTCGTGT -o /full/path/to/files/file_R1.C01.fastq.gz.adapterTrim.fastq.gz -p /full/path/to/files/file_R2.C01.fastq.gz.adapterTrim.fastq.gz /full/path/to/files/file_R1.C01.fastq.gz /full/path/to/files/file_R2.C01.fastq.gz > /full/path/to/files/file_R1.C01.fastq.gz.adapterTrim.metrics
  4. Takes output from cutadapt round 1.
  5. Run to trim off the 3’ adapters on read 2, to control for double ligation events.
  6. Command: cutadapt -f fastq --match-read-wildcards --times 1 -e 0.1 -O 5 --quality-cutoff 6 -m 18 -A AACTTGTAGATCGGA -A AGGACCAAGATCGGA -A ACTTGTAGATCGGAA -A GGACCAAGATCGGAA -A CTTGTAGATCGGAAG -A GACCAAGATCGGAAG -A TTGTAGATCGGAAGA -A ACCAAGATCGGAAGA -A TGTAGATCGGAAGAG -A CCAAGATCGGAAGAG -A GTAGATCGGAAGAGC -A CAAGATCGGAAGAGC -A TAGATCGGAAGAGCG -A AAGATCGGAAGAGCG -A AGATCGGAAGAGCGT -A GATCGGAAGAGCGTC -A ATCGGAAGAGCGTCG -A TCGGAAGAGCGTCGT -A CGGAAGAGCGTCGTG -A GGAAGAGCGTCGTGT -o /full/path/to/files/file_R1.C01.fastq.gz.adapterTrim.round2.fastq.gz -p /full/path/to/files/file_R2.C01.fastq.gz.adapterTrim.round2.fastq.gz /full/path/to/files/file_R1.C01.fastq.gz.adapterTrim.fastq.gz /full/path/to/files/file_R2.C01.fastq.gz.adapterTrim.fastq.gz > /full/path/to/files/file_R1.C01.fastq.gz.adapterTrim.round2.metrics
  7. Takes output from cutadapt round 2.
  8. Maps to human specific version of RepBase used to remove repetitive elements, helps control for spurious artifacts from rRNA (& other) repetitive reads.
Showing first 8 steps.

Supplementary Files (1)

GSE77629_RAW.tar Download
GEO Samples (24)

Dataset Citations (1)

Robust transcriptome-wide discovery of RNA-binding protein binding sites with enhanced CLIP (eCLIP).
PMID 27018577 · 2016 · Nature methods
Eric L Van Nostrand, Gabriel A Pratt, Alexander A Shishkin, Chelsea Gelboin-Burkhart, Mark Y Fang, Balaji Sundararaman, Steven M Blue, Thai B Nguyen, Christine Surka, Keri Elkins, Rebecca Stanton, Frank Rigo, Mitchell Guttman, Gene W Yeo

SRA Experiments (24) and Runs (41)

Total: 14297 MB
SRX1563178 SRP069351 OTHER PAIRED
GSM2055432: RBFOX2 293T Rep1; Homo sapiens; OTHER
Sample: SRS1277718
BioProject: PRJNA311053
BioSample: SAMN04461214
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: RBFOX2 293T
cell line: 293T
rip antibody: RBFOX2
antibody manufacturer: Bethyl
rnase i fragmentation condition: 40 U
Original files (1)
RBFOX2 293T
Runs (2)
Run Spots Bases Size (MB) Files Link
SRR3147598 35774827 3635417008 1506.31 293XT_CLIP_RBFOX2_1120_GAATTCGT-TAATCTTA_R1.A04_293XT_CLIP_RBFOX2_112… SRA
SRR3147599 28066781 2829503774 1139.5 293XT_CLIP_RBFOX2_1120_GAATTCGT-TAATCTTA_R1.F05_293XT_CLIP_RBFOX2_112… SRA
SRX1563179 SRP069351 OTHER PAIRED
GSM2055433: RBFOX2 293T Rep1 Input; Homo sapiens; OTHER
Sample: SRS1277717
BioProject: PRJNA311053
BioSample: SAMN04461215
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: RBFOX2 293T Input
cell line: 293T
rip antibody: none (input)
rnase i fragmentation condition: 40 U
Original files (1)
RBFOX2 293T Input
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3147600 32054523 3365724915 1098.43 293XT_Input_1120_GAATTCGT-GGCTCTGA_R1.unassigned.randomer.fastq.gz, 2… SRA
SRX1563180 SRP069351 OTHER PAIRED
GSM2055434: RBFOX2 293T Rep2; Homo sapiens; OTHER
Sample: SRS1277714
BioProject: PRJNA311053
BioSample: SAMN04461216
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: RBFOX2 293T
cell line: 293T
rip antibody: RBFOX2
antibody manufacturer: Bethyl
rnase i fragmentation condition: 40 U
Original files (1)
RBFOX2 293T
Runs (2)
Run Spots Bases Size (MB) Files Link
SRR3147601 57864589 5904213086 2492.24 293XT_CLIP_RBFOX2_0204_GAGATTCC-TAATCTTA_R1.A03_293XT_CLIP_RBFOX2_020… SRA
SRR3147602 12410277 1264635578 525.32 293XT_CLIP_RBFOX2_0204_GAGATTCC-TAATCTTA_R1.G07_293XT_CLIP_RBFOX2_020… SRA
SRX1563181 SRP069351 OTHER PAIRED
GSM2055435: RBFOX2 293T Rep2 Input; Homo sapiens; OTHER
Sample: SRS1277716
BioProject: PRJNA311053
BioSample: SAMN04461145
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: RBFOX2 293T Input
cell line: 293T
rip antibody: none (input)
rnase i fragmentation condition: 40 U
Original files (1)
RBFOX2 293T Input
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3147603 30095905 3160070025 1013.87 293XT_Input_0204_ATTCAGAA-TAATCTTA_R1.unassigned.randomer.fastq.gz, 2… SRA
SRX1563182 SRP069351 OTHER PAIRED
GSM2055436: IgG 293T Rep1; Homo sapiens; OTHER
Sample: SRS1277715
BioProject: PRJNA311053
BioSample: SAMN04461146
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: IgG 293T
cell line: 293T
rip antibody: IgG isotype control
antibody manufacturer: Thermo Fisher
rnase i fragmentation condition: 40 U
Original files (1)
IgG 293T
Runs (2)
Run Spots Bases Size (MB) Files Link
SRR3147604 7461940 701422360 216.94 293XT_CLIP_IgG_1120_ATTCAGAA-GGCTCTGA_R1.A04_293XT_CLIP_IgG_1120_Inpu… SRA
SRR3147605 9503931 893369514 276.2 293XT_CLIP_IgG_1120_ATTCAGAA-GGCTCTGA_R1.F05_293XT_CLIP_IgG_1120_Inpu… SRA
SRX1563183 SRP069351 OTHER PAIRED
GSM2055437: IgG 293T Rep1 Input; Homo sapiens; OTHER
Sample: SRS1277761
BioProject: PRJNA311053
BioSample: SAMN04461147
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: IgG 293T Input
cell line: 293T
rip antibody: none (input)
rnase i fragmentation condition: 40 U
Original files (1)
IgG 293T Input
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3147606 32759737 3439772385 1123.75 293XT_Input_IgG1120_GAGATTCC-GGCTCTGA_R1.unassigned.randomer.fastq.gz… SRA
SRX1563184 SRP069351 OTHER PAIRED
GSM2055438: SLBP 293T Rep1; Homo sapiens; OTHER
Sample: SRS1277760
BioProject: PRJNA311053
BioSample: SAMN04461148
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: SLBP 293T
cell line: 293T
rip antibody: SLBP
antibody manufacturer: MBL
rnase i fragmentation condition: 40 U
Original files (1)
SLBP 293T
Runs (2)
Run Spots Bases Size (MB) Files Link
SRR3147607 6932623 651666562 220.43 EVN_SLBP_pool4_ATTACTCG-CCTATCCT_L007_R1.A04_EVN_041315_CLIP12_SLBP.r… SRA
SRR3147608 5011852 471114088 157.28 EVN_SLBP_pool4_ATTACTCG-CCTATCCT_L007_R1.F05_EVN_041315_CLIP12_SLBP.r… SRA
SRX1563185 SRP069351 OTHER PAIRED
GSM2055439: SLBP 293T Rep1 Input; Homo sapiens; OTHER
Sample: SRS1277759
BioProject: PRJNA311053
BioSample: SAMN04461149
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: SLBP 293T Input
cell line: 293T
rip antibody: none (input)
rnase i fragmentation condition: 40 U
Original files (1)
SLBP 293T Input
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3147609 15956998 1675484790 585.38 EVN_INPUT_12_ATTACTCG-ATAGAGGC_L007_R1.unassigned.randomer.fastq.gz, … SRA
SRX1563186 SRP069351 OTHER PAIRED
GSM2055440: SLBP 293T Rep2; Homo sapiens; OTHER
Sample: SRS1277758
BioProject: PRJNA311053
BioSample: SAMN04461150
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: SLBP 293T
cell line: 293T
rip antibody: SLBP
antibody manufacturer: MBL
rnase i fragmentation condition: 40 U
Original files (1)
SLBP 293T
Runs (2)
Run Spots Bases Size (MB) Files Link
SRR3147610 6006271 564589474 191.4 EVN_SLBP_pool4_ATTACTCG-CCTATCCT_L007_R1.A03_EVN_041315_CLIP13_SLBP.r… SRA
SRR3147611 3171880 298156720 101.46 EVN_SLBP_pool4_ATTACTCG-CCTATCCT_L007_R1.G07_EVN_041315_CLIP13_SLBP.r… SRA
SRX1563187 SRP069351 OTHER PAIRED
GSM2055441: SLBP 293T Rep2 Input; Homo sapiens; OTHER
Sample: SRS1277756
BioProject: PRJNA311053
BioSample: SAMN04461167
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: SLBP 293T Input
cell line: 293T
rip antibody: none (input)
rnase i fragmentation condition: 40 U
Original files (1)
SLBP 293T Input
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3147612 12184394 1279361370 452.96 EVN_INPUT_13_TCCGGAGA-ATAGAGGC_L007_R1.unassigned.randomer.fastq.gz, … SRA
SRX1563188 SRP069351 OTHER PAIRED
GSM2055442: SLBP 293T Rep3; Homo sapiens; OTHER
Sample: SRS1277757
BioProject: PRJNA311053
BioSample: SAMN04461168
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: SLBP 293T
cell line: 293T
rip antibody: SLBP
antibody manufacturer: MBL
rnase i fragmentation condition: 5 U
Original files (1)
SLBP 293T
Runs (2)
Run Spots Bases Size (MB) Files Link
SRR3147613 3305241 323913618 106.71 EVN_SLBP_pool4_ATTACTCG-CCTATCCT_L007_R1.A01_EVN_041315_CLIP14_SLBP.r… SRA
SRR3147614 2574441 252295218 85.79 EVN_SLBP_pool4_ATTACTCG-CCTATCCT_L007_R1.B06_EVN_041315_CLIP14_SLBP.r… SRA
SRX1563189 SRP069351 OTHER PAIRED
GSM2055443: SLBP 293T Rep3 Input; Homo sapiens; OTHER
Sample: SRS1277755
BioProject: PRJNA311053
BioSample: SAMN04461169
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: SLBP 293T Input
cell line: 293T
rip antibody: none (input)
rnase i fragmentation condition: 5 U
Original files (1)
SLBP 293T Input
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3147615 14941475 1568854875 541.48 EVN_INPUT_14_CGCTCATT-TATAGCCT_L007_R1.unassigned.randomer.fastq.gz, … SRA
SRX1563190 SRP069351 OTHER PAIRED
GSM2055444: SLBP 293T Rep4; Homo sapiens; OTHER
Sample: SRS1277754
BioProject: PRJNA311053
BioSample: SAMN04461170
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: SLBP 293T
cell line: 293T
rip antibody: SLBP
antibody manufacturer: MBL
rnase i fragmentation condition: 5 U
Original files (1)
SLBP 293T
Runs (2)
Run Spots Bases Size (MB) Files Link
SRR3147616 2564636 251334328 82.89 EVN_SLBP_pool4_ATTACTCG-CCTATCCT_L007_R1.C01_EVN_041315_CLIP15_SLBP.r… SRA
SRR3147617 1564530 153323940 51.16 EVN_SLBP_pool4_ATTACTCG-CCTATCCT_L007_R1.D08fixed_EVN_041315_CLIP15_S… SRA
SRX1563191 SRP069351 OTHER PAIRED
GSM2055445: SLBP 293T Rep4 Input; Homo sapiens; OTHER
Sample: SRS1277753
BioProject: PRJNA311053
BioSample: SAMN04461171
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: SLBP 293T Input
cell line: 293T
rip antibody: none (input)
rnase i fragmentation condition: 5 U
Original files (1)
SLBP 293T Input
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR3147618 13340469 1400749245 479.04 EVN_INPUT_15_GAGATTCC-TATAGCCT_L007_R1.unassigned.randomer.fastq.gz, … SRA
SRX1563192 SRP069351 OTHER PAIRED
GSM2055446: RBFOX2 Shearing 293T Condition0U; Homo sapiens; OTHER
Sample: SRS1277752
BioProject: PRJNA311053
BioSample: SAMN04461172
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: RBFOX2 Shearing 293T Condition0U
cell line: 293T
rip antibody: RBFOX2
antibody manufacturer: Bethyl
rnase i fragmentation condition: 0 U
Original files (1)
RBFOX2 Shearing 293T Condition0U
Runs (2)
Run Spots Bases Size (MB) Files Link
SRR3147619 4286111 420038878 140.79 EVN_RBFOX2_pool3_TCCGGAGA-GGCTCTGA_L007_R1.A01_EVN_041315_CLIP10_RBFO… SRA
SRR3147620 5048374 494740652 171.3 EVN_RBFOX2_pool3_TCCGGAGA-GGCTCTGA_L007_R1.B06_EVN_041315_CLIP10_RBFO… SRA
SRX1563193 SRP069351 OTHER PAIRED
GSM2055447: RBFOX2 Shearing 293T Condition5U Rep1; Homo sapiens; OTHER
Sample: SRS1277751
BioProject: PRJNA311053
BioSample: SAMN04461173
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: RBFOX2 Shearing 293T Condition5U
cell line: 293T
rip antibody: RBFOX2
antibody manufacturer: Bethyl
rnase i fragmentation condition: 5 U
Original files (1)
RBFOX2 Shearing 293T Condition5U
Runs (2)
Run Spots Bases Size (MB) Files Link
SRR3147621 1562672 153141856 51.08 EVN_RBFOX2_pool2_ATTACTCG-GGCTCTGA_L007_R1.A01_EVN_041315_CLIP8_RBFOX… SRA
SRR3147622 2471304 242187792 83.76 EVN_RBFOX2_pool2_ATTACTCG-GGCTCTGA_L007_R1.B06_EVN_041315_CLIP8_RBFOX… SRA
SRX1563194 SRP069351 OTHER PAIRED
GSM2055448: RBFOX2 Shearing 293T Condition40U Rep1; Homo sapiens; OTHER
Sample: SRS1277750
BioProject: PRJNA311053
BioSample: SAMN04461174
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: RBFOX2 Shearing 293T Condition40U
cell line: 293T
rip antibody: RBFOX2
antibody manufacturer: Bethyl
rnase i fragmentation condition: 40 U
Original files (1)
RBFOX2 Shearing 293T Condition40U
Runs (2)
Run Spots Bases Size (MB) Files Link
SRR3147623 3531081 331921614 112.08 EVN_RBFOX2_pool1_TCCGGAGA-CCTATCCT_L007_R1.A04_EVN_041315_CLIP1_RBFOX… SRA
SRR3147624 1675108 157460152 52.47 EVN_RBFOX2_pool1_TCCGGAGA-CCTATCCT_L007_R1.F05_EVN_041315_CLIP1_RBFOX… SRA
SRX1563195 SRP069351 OTHER PAIRED
GSM2055449: RBFOX2 Shearing 293T Condition400U; Homo sapiens; OTHER
Sample: SRS1277749
BioProject: PRJNA311053
BioSample: SAMN04461175
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: RBFOX2 Shearing 293T Condition400U
cell line: 293T
rip antibody: RBFOX2
antibody manufacturer: Bethyl
rnase i fragmentation condition: 400 U
Original files (1)
RBFOX2 Shearing 293T Condition400U
Runs (2)
Run Spots Bases Size (MB) Files Link
SRR3147625 2840814 267036516 90.37 EVN_RBFOX2_pool2_ATTACTCG-GGCTCTGA_L007_R1.A04_EVN_041315_CLIP5_RBFOX… SRA
SRR3147626 3847990 361711060 121.4 EVN_RBFOX2_pool2_ATTACTCG-GGCTCTGA_L007_R1.F05_EVN_041315_CLIP5_RBFOX… SRA
SRX1563196 SRP069351 OTHER PAIRED
GSM2055450: RBFOX2 Shearing 293T Condition2000U; Homo sapiens; OTHER
Sample: SRS1277724
BioProject: PRJNA311053
BioSample: SAMN04461176
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: RBFOX2 Shearing 293T Condition2000U
cell line: 293T
rip antibody: RBFOX2
antibody manufacturer: Bethyl
rnase i fragmentation condition: 2000 U
Original files (1)
RBFOX2 Shearing 293T Condition2000U
Runs (2)
Run Spots Bases Size (MB) Files Link
SRR3147627 1870102 175789588 59.88 EVN_RBFOX2_pool2_ATTACTCG-GGCTCTGA_L007_R1.A03_EVN_041315_CLIP6_RBFOX… SRA
SRR3147628 826352 77677088 26.06 EVN_RBFOX2_pool2_ATTACTCG-GGCTCTGA_L007_R1.G07_EVN_041315_CLIP6_RBFOX… SRA
SRX1563197 SRP069351 OTHER PAIRED
GSM2055451: RBFOX2 Shearing 293T Condition5U Rep2; Homo sapiens; OTHER
Sample: SRS1277723
BioProject: PRJNA311053
BioSample: SAMN04461177
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: RBFOX2 Shearing 293T Condition5U
cell line: 293T
rip antibody: RBFOX2
antibody manufacturer: Bethyl
rnase i fragmentation condition: 5 U
Original files (1)
RBFOX2 Shearing 293T Condition5U
Runs (2)
Run Spots Bases Size (MB) Files Link
SRR3147629 1735555 171015827 79.56 FOX2gel-pool2_S2_R1.B06_RBFOX2_293XT_1to200_gel_RBFOX2.randomer.fastq… SRA
SRR3147630 1538330 145382845 66.58 FOX2gel-pool2_S2_R1.F05_RBFOX2_293XT_1to200_gel_RBFOX2.randomer.fastq… SRA
SRX1563198 SRP069351 OTHER PAIRED
GSM2055452: RBFOX2 Shearing 293T Condition10U; Homo sapiens; OTHER
Sample: SRS1277722
BioProject: PRJNA311053
BioSample: SAMN04461178
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: RBFOX2 Shearing 293T Condition10U
cell line: 293T
rip antibody: RBFOX2
antibody manufacturer: Bethyl
rnase i fragmentation condition: 10 U
Original files (1)
RBFOX2 Shearing 293T Condition10U
Runs (2)
Run Spots Bases Size (MB) Files Link
SRR3147631 2442487 240343066 108.57 FOX2gel-pool2_S2_R1.A01_RBFOX2_293XT_1to100_gel_RBFOX2.randomer.fastq… SRA
SRR3147632 3005746 283876475 129.32 FOX2gel-pool2_S2_R1.A03_RBFOX2_293XT_1to100_gel_RBFOX2.randomer.fastq… SRA
SRX1563199 SRP069351 OTHER PAIRED
GSM2055453: RBFOX2 Shearing 293T Condition20U; Homo sapiens; OTHER
Sample: SRS1277721
BioProject: PRJNA311053
BioSample: SAMN04461179
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: RBFOX2 Shearing 293T Condition20U
cell line: 293T
rip antibody: RBFOX2
antibody manufacturer: Bethyl
rnase i fragmentation condition: 20 U
Original files (1)
RBFOX2 Shearing 293T Condition20U
Runs (2)
Run Spots Bases Size (MB) Files Link
SRR3147633 1913824 188224097 84.23 FOX2gel-pool1_S1_R1.C01_RBFOX2_293XT_1to50_gel_RBFOX2.randomer.fastq.… SRA
SRR3147634 1900082 187037524 84.96 FOX2gel-pool1_S1_R1.D08fixed_RBFOX2_293XT_1to50_gel_RBFOX2.randomer.f… SRA
SRX1563200 SRP069351 OTHER PAIRED
GSM2055454: RBFOX2 Shearing 293T Condition40U Rep2; Homo sapiens; OTHER
Sample: SRS1277720
BioProject: PRJNA311053
BioSample: SAMN04461180
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: RBFOX2 Shearing 293T Condition40U
cell line: 293T
rip antibody: RBFOX2
antibody manufacturer: Bethyl
rnase i fragmentation condition: 40 U
Original files (1)
RBFOX2 Shearing 293T Condition40U
Runs (2)
Run Spots Bases Size (MB) Files Link
SRR3147635 1889168 185971000 85.98 FOX2gel-pool1_S1_R1.B06_RBFOX2_293XT_1to25_gel_RBFOX2.randomer.fastq.… SRA
SRR3147636 1997493 188524989 85.87 FOX2gel-pool1_S1_R1.F05_RBFOX2_293XT_1to25_gel_RBFOX2.randomer.fastq.… SRA
SRX1563201 SRP069351 OTHER PAIRED
GSM2055455: RBFOX2 Shearing 293T Condition80U; Homo sapiens; OTHER
Sample: SRS1277719
BioProject: PRJNA311053
BioSample: SAMN04461181
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Homo sapiens
Sample attributes
source_name: RBFOX2 Shearing 293T Condition80U
cell line: 293T
rip antibody: RBFOX2
antibody manufacturer: Bethyl
rnase i fragmentation condition: 80 U
Original files (1)
RBFOX2 Shearing 293T Condition80U
Runs (2)
Run Spots Bases Size (MB) Files Link
SRR3147637 2466944 241692610 109.3 FOX2gel-pool1_S1_R1.A01_RBFOX2_293XT_1to12_gel_RBFOX2.randomer.fastq.… SRA
SRR3147638 2447273 230225763 104.86 FOX2gel-pool1_S1_R1.A04_RBFOX2_293XT_1to12_gel_RBFOX2.randomer.fastq.… SRA

Linked Publications (1)

Data Files (82)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
— 293XT_CLIP_IgG_1120_ATTCAGAA-GGCTCTGA_R1.A04_293XT_CLI… OTHER 216.9 MB link
— 293XT_CLIP_IgG_1120_ATTCAGAA-GGCTCTGA_R1.F05_293XT_CLI… OTHER 276.2 MB link
— 293XT_CLIP_RBFOX2_0204_GAGATTCC-TAATCTTA_R1.A03_293XT_… OTHER 2.4 GB link
— 293XT_CLIP_RBFOX2_0204_GAGATTCC-TAATCTTA_R1.G07_293XT_… OTHER 525.3 MB link
— 293XT_CLIP_RBFOX2_1120_GAATTCGT-TAATCTTA_R1.A04_293XT_… OTHER 1.5 GB link
— 293XT_CLIP_RBFOX2_1120_GAATTCGT-TAATCTTA_R1.F05_293XT_… OTHER 1.1 GB link
— 293XT_Input_0204_ATTCAGAA-TAATCTTA_R1.unassigned.rando… OTHER 1013.9 MB link
— 293XT_Input_1120_GAATTCGT-GGCTCTGA_R1.unassigned.rando… OTHER 1.1 GB link
— 293XT_Input_IgG1120_GAGATTCC-GGCTCTGA_R1.unassigned.ra… OTHER 1.1 GB link
— EVN_INPUT_12_ATTACTCG-ATAGAGGC_L007_R1.unassigned.rand… OTHER 585.4 MB link
— EVN_INPUT_13_TCCGGAGA-ATAGAGGC_L007_R1.unassigned.rand… OTHER 453.0 MB link
— EVN_INPUT_14_CGCTCATT-TATAGCCT_L007_R1.unassigned.rand… OTHER 541.5 MB link
— EVN_INPUT_15_GAGATTCC-TATAGCCT_L007_R1.unassigned.rand… OTHER 479.0 MB link
— EVN_RBFOX2_pool1_TCCGGAGA-CCTATCCT_L007_R1.A04_EVN_041… OTHER 112.1 MB link
— EVN_RBFOX2_pool1_TCCGGAGA-CCTATCCT_L007_R1.F05_EVN_041… OTHER 52.5 MB link
— EVN_RBFOX2_pool2_ATTACTCG-GGCTCTGA_L007_R1.A01_EVN_041… OTHER 51.1 MB link
— EVN_RBFOX2_pool2_ATTACTCG-GGCTCTGA_L007_R1.A03_EVN_041… OTHER 59.9 MB link
— EVN_RBFOX2_pool2_ATTACTCG-GGCTCTGA_L007_R1.A04_EVN_041… OTHER 90.4 MB link
— EVN_RBFOX2_pool2_ATTACTCG-GGCTCTGA_L007_R1.B06_EVN_041… OTHER 83.8 MB link
— EVN_RBFOX2_pool2_ATTACTCG-GGCTCTGA_L007_R1.F05_EVN_041… OTHER 121.4 MB link
— EVN_RBFOX2_pool2_ATTACTCG-GGCTCTGA_L007_R1.G07_EVN_041… OTHER 26.1 MB link
— EVN_RBFOX2_pool3_TCCGGAGA-GGCTCTGA_L007_R1.A01_EVN_041… OTHER 140.8 MB link
— EVN_RBFOX2_pool3_TCCGGAGA-GGCTCTGA_L007_R1.B06_EVN_041… OTHER 171.3 MB link
— EVN_SLBP_pool4_ATTACTCG-CCTATCCT_L007_R1.A01_EVN_04131… OTHER 106.7 MB link
— EVN_SLBP_pool4_ATTACTCG-CCTATCCT_L007_R1.A03_EVN_04131… OTHER 191.4 MB link
— EVN_SLBP_pool4_ATTACTCG-CCTATCCT_L007_R1.A04_EVN_04131… OTHER 220.4 MB link
— EVN_SLBP_pool4_ATTACTCG-CCTATCCT_L007_R1.B06_EVN_04131… OTHER 85.8 MB link
— EVN_SLBP_pool4_ATTACTCG-CCTATCCT_L007_R1.C01_EVN_04131… OTHER 82.9 MB link
— EVN_SLBP_pool4_ATTACTCG-CCTATCCT_L007_R1.D08fixed_EVN_… OTHER 51.2 MB link
— EVN_SLBP_pool4_ATTACTCG-CCTATCCT_L007_R1.F05_EVN_04131… OTHER 157.3 MB link
— EVN_SLBP_pool4_ATTACTCG-CCTATCCT_L007_R1.G07_EVN_04131… OTHER 101.5 MB link
— FOX2gel-pool1_S1_R1.A01_RBFOX2_293XT_1to12_gel_RBFOX2.… OTHER 109.3 MB link
— FOX2gel-pool1_S1_R1.A04_RBFOX2_293XT_1to12_gel_RBFOX2.… OTHER 104.9 MB link
— FOX2gel-pool1_S1_R1.B06_RBFOX2_293XT_1to25_gel_RBFOX2.… OTHER 86.0 MB link
— FOX2gel-pool1_S1_R1.C01_RBFOX2_293XT_1to50_gel_RBFOX2.… OTHER 84.2 MB link
— FOX2gel-pool1_S1_R1.D08fixed_RBFOX2_293XT_1to50_gel_RB… OTHER 85.0 MB link
— FOX2gel-pool1_S1_R1.F05_RBFOX2_293XT_1to25_gel_RBFOX2.… OTHER 85.9 MB link
— FOX2gel-pool2_S2_R1.A01_RBFOX2_293XT_1to100_gel_RBFOX2… OTHER 108.6 MB link
— FOX2gel-pool2_S2_R1.A03_RBFOX2_293XT_1to100_gel_RBFOX2… OTHER 129.3 MB link
— FOX2gel-pool2_S2_R1.B06_RBFOX2_293XT_1to200_gel_RBFOX2… OTHER 79.6 MB link
— FOX2gel-pool2_S2_R1.F05_RBFOX2_293XT_1to200_gel_RBFOX2… OTHER 66.6 MB link
— SRR3147598_GSM2055432 OTHER 1.5 GB link
— SRR3147599_GSM2055432 OTHER 1.1 GB link
— SRR3147600_GSM2055433 OTHER 1.1 GB link
— SRR3147601_GSM2055434 OTHER 2.4 GB link
— SRR3147602_GSM2055434 OTHER 525.0 MB link
— SRR3147603_GSM2055435 OTHER 1013.0 MB link
— SRR3147604_GSM2055436 OTHER 216.0 MB link
— SRR3147605_GSM2055436 OTHER 276.0 MB link
— SRR3147606_GSM2055437 OTHER 1.1 GB link
— SRR3147607_GSM2055438 OTHER 220.0 MB link
— SRR3147608_GSM2055438 OTHER 157.0 MB link
— SRR3147609_GSM2055439 OTHER 585.0 MB link
— SRR3147610_GSM2055440 OTHER 191.0 MB link
— SRR3147611_GSM2055440 OTHER 101.0 MB link
— SRR3147612_GSM2055441 OTHER 452.0 MB link
— SRR3147613_GSM2055442 OTHER 106.0 MB link
— SRR3147614_GSM2055442 OTHER 85.0 MB link
— SRR3147615_GSM2055443 OTHER 541.0 MB link
— SRR3147616_GSM2055444 OTHER 82.0 MB link
— SRR3147617_GSM2055444 OTHER 51.0 MB link
— SRR3147618_GSM2055445 OTHER 479.0 MB link
— SRR3147619_GSM2055446 OTHER 140.0 MB link
— SRR3147620_GSM2055446 OTHER 171.0 MB link
— SRR3147621_GSM2055447 OTHER 51.0 MB link
— SRR3147622_GSM2055447 OTHER 83.0 MB link
— SRR3147623_GSM2055448 OTHER 112.0 MB link
— SRR3147624_GSM2055448 OTHER 52.0 MB link
— SRR3147625_GSM2055449 OTHER 90.0 MB link
— SRR3147626_GSM2055449 OTHER 121.0 MB link
— SRR3147627_GSM2055450 OTHER 59.0 MB link
— SRR3147628_GSM2055450 OTHER 26.0 MB link
— SRR3147629_GSM2055451 OTHER 79.0 MB link
— SRR3147630_GSM2055451 OTHER 66.0 MB link
— SRR3147631_GSM2055452 OTHER 108.0 MB link
— SRR3147632_GSM2055452 OTHER 129.0 MB link
— SRR3147633_GSM2055453 OTHER 84.0 MB link
— SRR3147634_GSM2055453 OTHER 84.0 MB link
— SRR3147635_GSM2055454 OTHER 85.0 MB link
— SRR3147636_GSM2055454 OTHER 85.0 MB link
— SRR3147637_GSM2055455 OTHER 109.0 MB link
— SRR3147638_GSM2055455 OTHER 104.0 MB link