GSE299099

GSE GEO

View on GEO Export SRA CSV

Transcriptome changes in MCF7 cells after treatment with NONO ligands and controls

Organism: Homo sapiens

Platform: GPL20301

Samples: 30

Experiment Types:

Expression profiling by high throughput sequencing

Submitted: Jun 05 2025

Last Updated: Jun 09 2025

Status: Public on Jun 09 2025

Contact: Garrett,Lee,Lindsey (Scripps Research)

Relations

BioProject: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA1272574

Summary

RNA-Sequencing to determine transcriptome changes in MCF7 cells after treatment with NONO ligands and controls. NONO knockout (sgNONO) or control (sgControl) cells were treated.

Overall Design

sgControl or sgNONO MCF7 cells were treated in biological triplicate with DMSO (0.1%) or (R, R)-GL-373 (20ÂµM) or (S, S)-GL-373 or (R)-SKBG-1(5ÂµM) or (S)-SKBG-1(5ÂµM) for 6 hours

Analysis (3 steps)

View Data Processing

Processing steps for GSE299099

Transcript abundance was quantified using Salmon [v1.3.0] with GENCODE v37 annotation.
Gene level quantification was performed using tximeta [v1.8.4].
Differential gene expression was analyzed by DESeq2 [v1.30.1]

Supplementary Files (30)

GSE299099_KO_373_R1.markdup.sorted.bam.txt.gz Download

GSE299099_KO_373_R2.markdup.sorted.bam.txt.gz Download

GSE299099_KO_373_R3.markdup.sorted.bam.txt.gz Download

GSE299099_KO_374_01.markdup.sorted.bam.txt.gz Download

GSE299099_KO_374_02.markdup.sorted.bam.txt.gz Download

GSE299099_KO_374_03.markdup.sorted.bam.txt.gz Download

GSE299099_KO_DMSO_R1.markdup.sorted.bam.txt.gz Download

GSE299099_KO_DMSO_R2.markdup.sorted.bam.txt.gz Download

GSE299099_KO_DMSO_R3.markdup.sorted.bam.txt.gz Download

GSE299099_KO_RSKBG1_R1.markdup.sorted.bam.txt.gz Download

GSE299099_KO_RSKBG1_R2.markdup.sorted.bam.txt.gz Download

GSE299099_KO_RSKBG1_R3.markdup.sorted.bam.txt.gz Download

GSE299099_KO_SSKBG1_R1.markdup.sorted.bam.txt.gz Download

GSE299099_KO_SSKBG1_R2.markdup.sorted.bam.txt.gz Download

GSE299099_KO_SSKBG1_R3.markdup.sorted.bam.txt.gz Download

GSE299099_WT_373_R1.markdup.sorted.bam.txt.gz Download

GSE299099_WT_373_R2.markdup.sorted.bam.txt.gz Download

GSE299099_WT_373_R3.markdup.sorted.bam.txt.gz Download

GSE299099_WT_374_01.markdup.sorted.bam.txt.gz Download

GSE299099_WT_374_02.markdup.sorted.bam.txt.gz Download

GSE299099_WT_374_03.markdup.sorted.bam.txt.gz Download

GSE299099_WT_DMSO_R1.markdup.sorted.bam.txt.gz Download

GSE299099_WT_DMSO_R2.markdup.sorted.bam.txt.gz Download

GSE299099_WT_DMSO_R3.markdup.sorted.bam.txt.gz Download

GSE299099_WT_RSKBG1_R1.markdup.sorted.bam.txt.gz Download

GSE299099_WT_RSKBG1_R2.markdup.sorted.bam.txt.gz Download

GSE299099_WT_RSKBG1_R3.markdup.sorted.bam.txt.gz Download

GSE299099_WT_SSKBG1_R1.markdup.sorted.bam.txt.gz Download

GSE299099_WT_SSKBG1_R2.markdup.sorted.bam.txt.gz Download

GSE299099_WT_SSKBG1_R3.markdup.sorted.bam.txt.gz Download

SRA Experiments (30) and Runs (30)

Total: 91812 MB

SRX29084861 SRP590540 RNA-Seq PAIRED

GSM9032907: Total_RNAseq_MCF7_sgControl_6hours_R,R-GL-373_Replicate_1; Homo sapiens; RNA-Seq

Sample: SRS25296990

BioProject: PRJNA1272574

BioSample: SAMN48916309

Platform: ILLUMINA

Instrument: Illumina HiSeq 4000

Organism: Homo sapiens

Sample attributes

source_name: Breast

tissue: Breast

cell line: MCF7

cell type: Breast carcinoma

genotype: single guide - Control

treatment: (R, R)-GL-373, 20µM, 6 hours

geo_loc_name: missing

collection_date: missing

Original files (1)

Breast

Runs (1)

Run	Spots	Bases	Size (MB)	Files	Link
SRR33873337	30894522	9268356600	2621.57	1-sgC-373_R1_001.fastq.gz, 1-sgC-373_R2_001.fastq.gz, SRR33873337, SR…	SRA

SRX29084862 SRP590540 RNA-Seq PAIRED

GSM9032908: Total_RNAseq_MCF7_sgControl_6hours_R,R-GL-373_Replicate_2; Homo sapiens; RNA-Seq

Sample: SRS25296991

BioProject: PRJNA1272574

BioSample: SAMN48916308

Platform: ILLUMINA

Instrument: Illumina HiSeq 4000

Organism: Homo sapiens

Sample attributes

source_name: Breast

tissue: Breast

cell line: MCF7

cell type: Breast carcinoma

genotype: single guide - Control

treatment: (R, R)-GL-373, 20µM, 6 hours

geo_loc_name: missing

collection_date: missing

Original files (1)

Breast

Runs (1)

Run	Spots	Bases	Size (MB)	Files	Link
SRR33873336	32954592	9886377600	2832.55	2-sgC-373_R1_001.fastq.gz, 2-sgC-373_R2_001.fastq.gz, SRR33873336, SR…	SRA

SRX29084863 SRP590540 RNA-Seq PAIRED

GSM9032909: Total_RNAseq_MCF7_sgControl_6hours_R,R-GL-373_Replicate_3; Homo sapiens; RNA-Seq

Sample: SRS25296992

BioProject: PRJNA1272574

BioSample: SAMN48916307

Platform: ILLUMINA

Instrument: Illumina HiSeq 4000

Organism: Homo sapiens

Sample attributes

source_name: Breast

tissue: Breast

cell line: MCF7

cell type: Breast carcinoma

genotype: single guide - Control

treatment: (R, R)-GL-373, 20µM, 6 hours

geo_loc_name: missing

collection_date: missing

Original files (1)

Breast

Runs (1)

Run	Spots	Bases	Size (MB)	Files	Link
SRR33873335	38469123	11540736900	3342.26	3-sgC-373_R1_001.fastq.gz, 3-sgC-373_R2_001.fastq.gz, SRR33873335, SR…	SRA

SRX29084864 SRP590540 RNA-Seq PAIRED

GSM9032910: Total_RNAseq_MCF7_sgControl_6hours_S,S-GL-373_Replicate_1; Homo sapiens; RNA-Seq

Sample: SRS25296993

BioProject: PRJNA1272574

BioSample: SAMN48916306

Platform: ILLUMINA

Instrument: Illumina HiSeq 4000

Organism: Homo sapiens

Sample attributes

source_name: Breast

tissue: Breast

cell line: MCF7

cell type: Breast carcinoma

genotype: single guide - Control

treatment: (S, S)-GL-373, 20µM, 6 hours

geo_loc_name: missing

collection_date: missing

Original files (1)

Breast

Runs (1)

Run	Spots	Bases	Size (MB)	Files	Link
SRR33873334	31801505	9540451500	2754.82	1-sgC-374_R1_001.fastq.gz, 1-sgC-374_R2_001.fastq.gz, SRR33873334, SR…	SRA

SRX29084865 SRP590540 RNA-Seq PAIRED

GSM9032911: Total_RNAseq_MCF7_sgControl_6hours_S,S-GL-373_Replicate_2; Homo sapiens; RNA-Seq

Sample: SRS25296994

BioProject: PRJNA1272574

BioSample: SAMN48916305

Platform: ILLUMINA

Instrument: Illumina HiSeq 4000

Organism: Homo sapiens

Sample attributes

source_name: Breast

tissue: Breast

cell line: MCF7

cell type: Breast carcinoma

genotype: single guide - Control

treatment: (S, S)-GL-373, 20µM, 6 hours

geo_loc_name: missing

collection_date: missing

Original files (1)

Breast

Runs (1)

Run	Spots	Bases	Size (MB)	Files	Link
SRR33873333	32529956	9758986800	2815.96	SRR33873333.lite, SRR33873333, 2-sgC-374_R2_001.fastq.gz, 2-sgC-374_R…	SRA

SRX29084866 SRP590540 RNA-Seq PAIRED

GSM9032912: Total_RNAseq_MCF7_sgControl_6hours_S,S-GL-373_Replicate_3; Homo sapiens; RNA-Seq

Sample: SRS25296995

BioProject: PRJNA1272574

BioSample: SAMN48916304

Platform: ILLUMINA

Instrument: Illumina HiSeq 4000

Organism: Homo sapiens

Sample attributes

source_name: Breast

tissue: Breast

cell line: MCF7

cell type: Breast carcinoma

genotype: single guide - Control

treatment: (S, S)-GL-373, 20µM, 6 hours

geo_loc_name: missing

collection_date: missing

Original files (1)

Breast

Runs (1)

Run	Spots	Bases	Size (MB)	Files	Link
SRR33873332	40755769	12226730700	3480.9	3-sgC-374_R1_001.fastq.gz, 3-sgC-374_R2_001.fastq.gz, SRR33873332, SR…	SRA

SRX29084867 SRP590540 RNA-Seq PAIRED

GSM9032913: Total_RNAseq_MCF7_sgControl_6hours_DMSO_Replicate_1; Homo sapiens; RNA-Seq

Sample: SRS25296996

BioProject: PRJNA1272574

BioSample: SAMN48916303

Platform: ILLUMINA

Instrument: Illumina HiSeq 4000

Organism: Homo sapiens

Sample attributes

source_name: Breast

tissue: Breast

cell line: MCF7

cell type: Breast carcinoma

genotype: single guide - Control

treatment: DMSO, 6 hours

geo_loc_name: missing

collection_date: missing

Original files (1)

Breast

Runs (1)

Run	Spots	Bases	Size (MB)	Files	Link
SRR33873331	35830529	10749158700	3045.19	1-sgC-DMSO_R1_001.fastq.gz, 1-sgC-DMSO_R2_001.fastq.gz, SRR33873331, …	SRA

SRX29084868 SRP590540 RNA-Seq PAIRED

GSM9032914: Total_RNAseq_MCF7_sgControl_6hours_DMSO_Replicate_2; Homo sapiens; RNA-Seq

Sample: SRS25296997

BioProject: PRJNA1272574

BioSample: SAMN48916302

Platform: ILLUMINA

Instrument: Illumina HiSeq 4000

Organism: Homo sapiens

Sample attributes

source_name: Breast

tissue: Breast

cell line: MCF7

cell type: Breast carcinoma

genotype: single guide - Control

treatment: DMSO, 6 hours

geo_loc_name: missing

collection_date: missing

Original files (1)

Breast

Runs (1)

Run	Spots	Bases	Size (MB)	Files	Link
SRR33873330	34596747	10379024100	2954.38	SRR33873330.lite, SRR33873330, 2-sgC-DMSO_R2_001.fastq.gz, 2-sgC-DMSO…	SRA

SRX29084869 SRP590540 RNA-Seq PAIRED

GSM9032915: Total_RNAseq_MCF7_sgControl_6hours_DMSO_Replicate_3; Homo sapiens; RNA-Seq

Sample: SRS25296998

BioProject: PRJNA1272574

BioSample: SAMN48916301

Platform: ILLUMINA

Instrument: Illumina HiSeq 4000

Organism: Homo sapiens

Sample attributes

source_name: Breast

tissue: Breast

cell line: MCF7

cell type: Breast carcinoma

genotype: single guide - Control

treatment: DMSO, 6 hours

geo_loc_name: missing

collection_date: missing

Original files (1)

Breast

Runs (1)

Run	Spots	Bases	Size (MB)	Files	Link
SRR33873329	29527800	8858340000	2496.21	3-sgC-DMSO_R1_001.fastq.gz, 3-sgC-DMSO_R2_001.fastq.gz, SRR33873329, …	SRA

SRX29084870 SRP590540 RNA-Seq PAIRED

GSM9032916: Total_RNAseq_MCF7_sgControl_6hours_R-SKBG-1_Replicate_1; Homo sapiens; RNA-Seq

Sample: SRS25296999

BioProject: PRJNA1272574

BioSample: SAMN48916300

Platform: ILLUMINA

Instrument: Illumina HiSeq 4000

Organism: Homo sapiens

Sample attributes

source_name: Breast

tissue: Breast

cell line: MCF7

cell type: Breast carcinoma

genotype: single guide - Control

treatment: (R)-SKBG-1, 20µM, 6 hours

geo_loc_name: missing

collection_date: missing

Original files (1)

Breast

Runs (1)

Run	Spots	Bases	Size (MB)	Files	Link
SRR33873328	33819404	10145821200	2891.07	1-sgC-R_R1_001.fastq.gz, 1-sgC-R_R2_001.fastq.gz, SRR33873328, SRR338…	SRA

SRX29084871 SRP590540 RNA-Seq PAIRED

GSM9032917: Total_RNAseq_MCF7_sgControl_6hours_R-SKBG-1_Replicate_2; Homo sapiens; RNA-Seq

Sample: SRS25297000

BioProject: PRJNA1272574

BioSample: SAMN48916299

Platform: ILLUMINA

Instrument: Illumina HiSeq 4000

Organism: Homo sapiens

Sample attributes

source_name: Breast

tissue: Breast

cell line: MCF7

cell type: Breast carcinoma

genotype: single guide - Control

treatment: (R)-SKBG-1, 20µM, 6 hours

geo_loc_name: missing

collection_date: missing

Original files (1)

Breast

Runs (1)

Run	Spots	Bases	Size (MB)	Files	Link
SRR33873327	41502620	12450786000	3551.41	2-sgC-R_R1_001.fastq.gz, 2-sgC-R_R2_001.fastq.gz, SRR33873327, SRR338…	SRA

SRX29084872 SRP590540 RNA-Seq PAIRED

GSM9032918: Total_RNAseq_MCF7_sgControl_6hours_R-SKBG-1_Replicate_3; Homo sapiens; RNA-Seq

Sample: SRS25297001

BioProject: PRJNA1272574

BioSample: SAMN48916298

Platform: ILLUMINA

Instrument: Illumina HiSeq 4000

Organism: Homo sapiens

Sample attributes

source_name: Breast

tissue: Breast

cell line: MCF7

cell type: Breast carcinoma

genotype: single guide - Control

treatment: (R)-SKBG-1, 20µM, 6 hours

geo_loc_name: missing

collection_date: missing

Original files (1)

Breast

Runs (1)

Run	Spots	Bases	Size (MB)	Files	Link
SRR33873326	35425243	10627572900	3011.08	3-sgC-R_R1_001.fastq.gz, 3-sgC-R_R2_001.fastq.gz, SRR33873326, SRR338…	SRA

SRX29084873 SRP590540 RNA-Seq PAIRED

GSM9032919: Total_RNAseq_MCF7_sgControl_6hours_S-SKBG-1_Replicate_1; Homo sapiens; RNA-Seq

Sample: SRS25297002

BioProject: PRJNA1272574

BioSample: SAMN48916297

Platform: ILLUMINA

Instrument: Illumina HiSeq 4000

Organism: Homo sapiens

Sample attributes

source_name: Breast

tissue: Breast

cell line: MCF7

cell type: Breast carcinoma

genotype: single guide - Control

treatment: (S)-SKBG-1, 20µM, 6 hours

geo_loc_name: missing

collection_date: missing

Original files (1)

Breast

Runs (1)

Run	Spots	Bases	Size (MB)	Files	Link
SRR33873325	39335770	11800731000	3353.24	1-sgC-S_R1_001.fastq.gz, 1-sgC-S_R2_001.fastq.gz, SRR33873325, SRR338…	SRA

SRX29084874 SRP590540 RNA-Seq PAIRED

GSM9032920: Total_RNAseq_MCF7_sgControl_6hours_S-SKBG-1_Replicate_2; Homo sapiens; RNA-Seq

Sample: SRS25297003

BioProject: PRJNA1272574

BioSample: SAMN48916296

Platform: ILLUMINA

Instrument: Illumina HiSeq 4000

Organism: Homo sapiens

Sample attributes

source_name: Breast

tissue: Breast

cell line: MCF7

cell type: Breast carcinoma

genotype: single guide - Control

treatment: (S)-SKBG-1, 20µM, 6 hours

geo_loc_name: missing

collection_date: missing

Original files (1)

Breast

Runs (1)

Run	Spots	Bases	Size (MB)	Files	Link
SRR33873324	35140702	10542210600	3013.72	2-sgC-S_R1_001.fastq.gz, 2-sgC-S_R2_001.fastq.gz, SRR33873324, SRR338…	SRA

SRX29084875 SRP590540 RNA-Seq PAIRED

GSM9032921: Total_RNAseq_MCF7_sgControl_6hours_S-SKBG-1_Replicate_3; Homo sapiens; RNA-Seq

Sample: SRS25297004

BioProject: PRJNA1272574

BioSample: SAMN48916295

Platform: ILLUMINA

Instrument: Illumina HiSeq 4000

Organism: Homo sapiens

Sample attributes

source_name: Breast

tissue: Breast

cell line: MCF7

cell type: Breast carcinoma

genotype: single guide - Control

treatment: (S)-SKBG-1, 20µM, 6 hours

geo_loc_name: missing

collection_date: missing

Original files (1)

Breast

Runs (1)

Run	Spots	Bases	Size (MB)	Files	Link
SRR33873323	29227748	8768324400	2501.46	3-sgC-S_R1_001.fastq.gz, 3-sgC-S_R2_001.fastq.gz, SRR33873323, SRR338…	SRA

SRX29084876 SRP590540 RNA-Seq PAIRED

GSM9032922: Total_RNAseq_MCF7_sgNONO_6hours_R,R-GL-373_Replicate_1; Homo sapiens; RNA-Seq

Sample: SRS25297005

BioProject: PRJNA1272574

BioSample: SAMN48916294

Platform: ILLUMINA

Instrument: Illumina HiSeq 4000

Organism: Homo sapiens

Sample attributes

source_name: Breast

tissue: Breast

cell line: MCF7

cell type: Breast carcinoma

genotype: single guide - NONO_KO

treatment: (R, R)-GL-373, 20µM, 6 hours

geo_loc_name: missing

collection_date: missing

Original files (1)

Breast

Runs (1)

Run	Spots	Bases	Size (MB)	Files	Link
SRR33873322	38223685	11467105500	3276.61	1-sgN-373_R1_001.fastq.gz, 1-sgN-373_R2_001.fastq.gz, SRR33873322, SR…	SRA

SRX29084877 SRP590540 RNA-Seq PAIRED

GSM9032923: Total_RNAseq_MCF7_sgNONO_6hours_R,R-GL-373_Replicate_2; Homo sapiens; RNA-Seq

Sample: SRS25297006

BioProject: PRJNA1272574

BioSample: SAMN48916293

Platform: ILLUMINA

Instrument: Illumina HiSeq 4000

Organism: Homo sapiens

Sample attributes

source_name: Breast

tissue: Breast

cell line: MCF7

cell type: Breast carcinoma

genotype: single guide - NONO_KO

treatment: (R, R)-GL-373, 20µM, 6 hours

geo_loc_name: missing

collection_date: missing

Original files (1)

Breast

Runs (1)

Run	Spots	Bases	Size (MB)	Files	Link
SRR33873321	36626258	10987877400	3123.12	2-sgN-373_R1_001.fastq.gz, 2-sgN-373_R2_001.fastq.gz, SRR33873321, SR…	SRA

SRX29084878 SRP590540 RNA-Seq PAIRED

GSM9032924: Total_RNAseq_MCF7_sgNONO_6hours_R,R-GL-373_Replicate_3; Homo sapiens; RNA-Seq

Sample: SRS25297007

BioProject: PRJNA1272574

BioSample: SAMN48916292

Platform: ILLUMINA

Instrument: Illumina HiSeq 4000

Organism: Homo sapiens

Sample attributes

source_name: Breast

tissue: Breast

cell line: MCF7

cell type: Breast carcinoma

genotype: single guide - NONO_KO

treatment: (R, R)-GL-373, 20µM, 6 hours

geo_loc_name: missing

collection_date: missing

Original files (1)

Breast

Runs (1)

Run	Spots	Bases	Size (MB)	Files	Link
SRR33873320	45055320	13516596000	3819.64	3-sgN-373_R1_001.fastq.gz, 3-sgN-373_R2_001.fastq.gz, SRR33873320, SR…	SRA

SRX29084879 SRP590540 RNA-Seq PAIRED

GSM9032925: Total_RNAseq_MCF7_sgNONO_6hours_S,S-GL-373_Replicate_1; Homo sapiens; RNA-Seq

Sample: SRS25297008

BioProject: PRJNA1272574

BioSample: SAMN48916291

Platform: ILLUMINA

Instrument: Illumina HiSeq 4000

Organism: Homo sapiens

Sample attributes

source_name: Breast

tissue: Breast

cell line: MCF7

cell type: Breast carcinoma

genotype: single guide - NONO_KO

treatment: (S, S)-GL-373, 20µM, 6 hours

geo_loc_name: missing

collection_date: missing

Original files (1)

Breast

Runs (1)

Run	Spots	Bases	Size (MB)	Files	Link
SRR33873319	42639592	12791877600	3683.47	1-sgN-374_R1_001.fastq.gz, 1-sgN-374_R2_001.fastq.gz, SRR33873319, SR…	SRA

SRX29084880 SRP590540 RNA-Seq PAIRED

GSM9032926: Total_RNAseq_MCF7_sgNONO_6hours_S,S-GL-373_Replicate_2; Homo sapiens; RNA-Seq

Sample: SRS25297009

BioProject: PRJNA1272574

BioSample: SAMN48916290

Platform: ILLUMINA

Instrument: Illumina HiSeq 4000

Organism: Homo sapiens

Sample attributes

source_name: Breast

tissue: Breast

cell line: MCF7

cell type: Breast carcinoma

genotype: single guide - NONO_KO

treatment: (S, S)-GL-373, 20µM , 6 hours

geo_loc_name: missing

collection_date: missing

Original files (1)

Breast

Runs (1)

Run	Spots	Bases	Size (MB)	Files	Link
SRR33873318	39014383	11704314900	3361.91	2-sgN-374_R1_001.fastq.gz, 2-sgN-374_R2_001.fastq.gz, SRR33873318, SR…	SRA

SRX29084881 SRP590540 RNA-Seq PAIRED

GSM9032927: Total_RNAseq_MCF7_sgNONO_6hours_S,S-GL-373_Replicate_3; Homo sapiens; RNA-Seq

Sample: SRS25297010

BioProject: PRJNA1272574

BioSample: SAMN48916289

Platform: ILLUMINA

Instrument: Illumina HiSeq 4000

Organism: Homo sapiens

Sample attributes

source_name: Breast

tissue: Breast

cell line: MCF7

cell type: Breast carcinoma

genotype: single guide - NONO_KO

treatment: (S, S)-GL-373, 20µM, 6 hours

geo_loc_name: missing

collection_date: missing

Original files (1)

Breast

Runs (1)

Run	Spots	Bases	Size (MB)	Files	Link
SRR33873317	34203146	10260943800	2975.93	SRR33873317.lite, SRR33873317, 3-sgN-374_R2_001.fastq.gz, 3-sgN-374_R…	SRA

SRX29084882 SRP590540 RNA-Seq PAIRED

GSM9032928: Total_RNAseq_MCF7_sgNONO_6hours_DMSO_Replicate_1; Homo sapiens; RNA-Seq

Sample: SRS25297011

BioProject: PRJNA1272574

BioSample: SAMN48916288

Platform: ILLUMINA

Instrument: Illumina HiSeq 4000

Organism: Homo sapiens

Sample attributes

source_name: Breast

tissue: Breast

cell line: MCF7

cell type: Breast carcinoma

genotype: single guide - NONO_KO

treatment: DMSO

geo_loc_name: missing

collection_date: missing

Original files (1)

Breast

Runs (1)

Run	Spots	Bases	Size (MB)	Files	Link
SRR33873316	32863872	9859161600	2793.42	SRR33873316.lite, SRR33873316, 1-sgN-DMSO_R2_001.fastq.gz, 1-sgN-DMSO…	SRA

SRX29084883 SRP590540 RNA-Seq PAIRED

GSM9032929: Total_RNAseq_MCF7_sgNONO_6hours_DMSO_Replicate_2; Homo sapiens; RNA-Seq

Sample: SRS25297012

BioProject: PRJNA1272574

BioSample: SAMN48916287

Platform: ILLUMINA

Instrument: Illumina HiSeq 4000

Organism: Homo sapiens

Sample attributes

source_name: Breast

tissue: Breast

cell line: MCF7

cell type: Breast carcinoma

genotype: single guide - NONO_KO

treatment: DMSO

geo_loc_name: missing

collection_date: missing

Original files (1)

Breast

Runs (1)

Run	Spots	Bases	Size (MB)	Files	Link
SRR33873315	37854211	11356263300	3236.96	2-sgN-DMSO_R1_001.fastq.gz, 2-sgN-DMSO_R2_001.fastq.gz, SRR33873315, …	SRA

SRX29084884 SRP590540 RNA-Seq PAIRED

GSM9032930: Total_RNAseq_MCF7_sgNONO_6hours_DMSO_Replicate_3; Homo sapiens; RNA-Seq

Sample: SRS25297013

BioProject: PRJNA1272574

BioSample: SAMN48916286

Platform: ILLUMINA

Instrument: Illumina HiSeq 4000

Organism: Homo sapiens

Sample attributes

source_name: Breast

tissue: Breast

cell line: MCF7

cell type: Breast carcinoma

genotype: single guide - NONO_KO

treatment: DMSO

geo_loc_name: missing

collection_date: missing

Original files (1)

Breast

Runs (1)

Run	Spots	Bases	Size (MB)	Files	Link
SRR33873314	35978613	10793583900	3057.57	SRR33873314.lite, SRR33873314, 3-sgN-DMSO_R2_001.fastq.gz, 3-sgN-DMSO…	SRA

SRX29084885 SRP590540 RNA-Seq PAIRED

GSM9032931: Total_RNAseq_MCF7_sgNONO_6hours_R-SKBG-1_Replicate_1; Homo sapiens; RNA-Seq

Sample: SRS25297014

BioProject: PRJNA1272574

BioSample: SAMN48916285

Platform: ILLUMINA

Instrument: Illumina HiSeq 4000

Organism: Homo sapiens

Sample attributes

source_name: Breast

tissue: Breast

cell line: MCF7

cell type: Breast carcinoma

genotype: single guide - NONO_KO

treatment: (R)-SKBG-1, 20µM, 6 hours

geo_loc_name: missing

collection_date: missing

Original files (1)

Breast

Runs (1)

Run	Spots	Bases	Size (MB)	Files	Link
SRR33873313	40435100	12130530000	3445.22	1-sgN-R_R1_001.fastq.gz, 1-sgN-R_R2_001.fastq.gz, SRR33873313, SRR338…	SRA

SRX29084886 SRP590540 RNA-Seq PAIRED

GSM9032932: Total_RNAseq_MCF7_sgNONO_6hours_R-SKBG-1_Replicate_2; Homo sapiens; RNA-Seq

Sample: SRS25297015

BioProject: PRJNA1272574

BioSample: SAMN48916284

Platform: ILLUMINA

Instrument: Illumina HiSeq 4000

Organism: Homo sapiens

Sample attributes

source_name: Breast

tissue: Breast

cell line: MCF7

cell type: Breast carcinoma

genotype: single guide - NONO_KO

treatment: (R)-SKBG-1, 20µM, 6 hours

geo_loc_name: missing

collection_date: missing

Original files (1)

Breast

Runs (1)

Run	Spots	Bases	Size (MB)	Files	Link
SRR33873312	39080376	11724112800	3385.31	2-sgN-R_R1_001.fastq.gz, 2-sgN-R_R2_001.fastq.gz, SRR33873312, SRR338…	SRA

SRX29084887 SRP590540 RNA-Seq PAIRED

GSM9032933: Total_RNAseq_MCF7_sgNONO_6hours_R-SKBG-1_Replicate_3; Homo sapiens; RNA-Seq

Sample: SRS25297016

BioProject: PRJNA1272574

BioSample: SAMN48916283

Platform: ILLUMINA

Instrument: Illumina HiSeq 4000

Organism: Homo sapiens

Sample attributes

source_name: Breast

tissue: Breast

cell line: MCF7

cell type: Breast carcinoma

genotype: single guide - NONO_KO

treatment: (R)-SKBG-1, 20µM, 6 hours

geo_loc_name: missing

collection_date: missing

Original files (1)

Breast

Runs (1)

Run	Spots	Bases	Size (MB)	Files	Link
SRR33873311	28961329	8688398700	2472.91	3-sgN-R_R1_001.fastq.gz, 3-sgN-R_R2_001.fastq.gz, SRR33873311, SRR338…	SRA

SRX29084888 SRP590540 RNA-Seq PAIRED

GSM9032934: Total_RNAseq_MCF7_sgNONO_6hours_S-SKBG-1_Replicate_1; Homo sapiens; RNA-Seq

Sample: SRS25297017

BioProject: PRJNA1272574

BioSample: SAMN48916282

Platform: ILLUMINA

Instrument: Illumina HiSeq 4000

Organism: Homo sapiens

Sample attributes

source_name: Breast

tissue: Breast

cell line: MCF7

cell type: Breast carcinoma

genotype: single guide - NONO_KO

treatment: (S)-SKBG-1, 20µM, 6 hours

geo_loc_name: missing

collection_date: missing

Original files (1)

Breast

Runs (1)

Run	Spots	Bases	Size (MB)	Files	Link
SRR33873310	34392475	10317742500	2940.41	1-sgN-S_R1_001.fastq.gz, 1-sgN-S_R2_001.fastq.gz, SRR33873310, SRR338…	SRA

SRX29084889 SRP590540 RNA-Seq PAIRED

GSM9032935: Total_RNAseq_MCF7_sgNONO_6hours_S-SKBG-1_Replicate_2; Homo sapiens; RNA-Seq

Sample: SRS25297018

BioProject: PRJNA1272574

BioSample: SAMN48916281

Platform: ILLUMINA

Instrument: Illumina HiSeq 4000

Organism: Homo sapiens

Sample attributes

source_name: Breast

tissue: Breast

cell line: MCF7

cell type: Breast carcinoma

genotype: single guide - NONO_KO

treatment: (S)-SKBG-1, 20µM , 6 hours

geo_loc_name: missing

collection_date: missing

Original files (1)

Breast

Runs (1)

Run	Spots	Bases	Size (MB)	Files	Link
SRR33873309	33791260	10137378000	2870.38	2-sgN-S_R1_001.fastq.gz, 2-sgN-S_R2_001.fastq.gz, SRR33873309, SRR338…	SRA

SRX29084890 SRP590540 RNA-Seq PAIRED

GSM9032936: Total_RNAseq_MCF7_sgNONO_6hours_S-SKBG-1_Replicate_3; Homo sapiens; RNA-Seq

Sample: SRS25297019

BioProject: PRJNA1272574

BioSample: SAMN48916280

Platform: ILLUMINA

Instrument: Illumina HiSeq 4000

Organism: Homo sapiens

Sample attributes

source_name: Breast

tissue: Breast

cell line: MCF7

cell type: Breast carcinoma

genotype: single guide - NONO_KO

treatment: (S)-SKBG-1, 20µM, 6 hours

geo_loc_name: missing

collection_date: missing

Original files (1)

Breast

Runs (1)

Run	Spots	Bases	Size (MB)	Files	Link
SRR33873308	31788637	9536591100	2703.68	SRR33873308.lite, SRR33873308, 3-sgN-S_R2_001.fastq.gz, 3-sgN-S_R1_00…	SRA

Linked Publications (2)

Structural and mechanistic analysis of covalent ligands targeting the RNA-binding protein NONO.

Cell chemical biology · 2026

Structural and mechanistic analysis of covalent ligands targeting the RNA-binding protein NONO.

Cell chemical biology · 2026

Data Files (30)

Accession	File Name	Stored Type	Size	Download
—	1-sgC-373_R1_001.fastq.gz	RNA-Seq	2.6 GB	link
—	1-sgC-374_R1_001.fastq.gz	RNA-Seq	2.7 GB	link
—	1-sgC-DMSO_R1_001.fastq.gz	RNA-Seq	3.0 GB	link
—	1-sgC-R_R1_001.fastq.gz	RNA-Seq	2.8 GB	link
—	1-sgC-S_R1_001.fastq.gz	RNA-Seq	3.3 GB	link
—	1-sgN-373_R1_001.fastq.gz	RNA-Seq	3.2 GB	link
—	1-sgN-374_R1_001.fastq.gz	RNA-Seq	3.6 GB	link
—	1-sgN-R_R1_001.fastq.gz	RNA-Seq	3.4 GB	link
—	1-sgN-S_R1_001.fastq.gz	RNA-Seq	2.9 GB	link
—	2-sgC-373_R1_001.fastq.gz	RNA-Seq	2.8 GB	link
—	2-sgC-R_R1_001.fastq.gz	RNA-Seq	3.5 GB	link
—	2-sgC-S_R1_001.fastq.gz	RNA-Seq	2.9 GB	link
—	2-sgN-373_R1_001.fastq.gz	RNA-Seq	3.0 GB	link
—	2-sgN-374_R1_001.fastq.gz	RNA-Seq	3.3 GB	link
—	2-sgN-DMSO_R1_001.fastq.gz	RNA-Seq	3.2 GB	link
—	2-sgN-R_R1_001.fastq.gz	RNA-Seq	3.3 GB	link
—	2-sgN-S_R1_001.fastq.gz	RNA-Seq	2.8 GB	link
—	3-sgC-373_R1_001.fastq.gz	RNA-Seq	3.3 GB	link
—	3-sgC-374_R1_001.fastq.gz	RNA-Seq	3.4 GB	link
—	3-sgC-DMSO_R1_001.fastq.gz	RNA-Seq	2.4 GB	link
—	3-sgC-R_R1_001.fastq.gz	RNA-Seq	2.9 GB	link
—	3-sgC-S_R1_001.fastq.gz	RNA-Seq	2.4 GB	link
—	3-sgN-373_R1_001.fastq.gz	RNA-Seq	3.7 GB	link
—	3-sgN-R_R1_001.fastq.gz	RNA-Seq	2.4 GB	link
—	SRR33873308.lite	RNA-Seq	2.6 GB	link
—	SRR33873314.lite	RNA-Seq	3.0 GB	link
—	SRR33873316.lite	RNA-Seq	2.7 GB	link
—	SRR33873317.lite	RNA-Seq	2.9 GB	link
—	SRR33873330.lite	RNA-Seq	2.9 GB	link
—	SRR33873333.lite	RNA-Seq	2.7 GB	link