← All datasets

ENCSR700FWD

ENCSR ENCODE
Export SRA CSV View on ENCODE

RNA-seq on HepG2 cells treated with a CRISPR gRNA against SMAD4. (SMAD4-BGHcLV18)

Organism: Homo sapiens
Experiment Types:
CRISPR RNA-seq
Status: released
Contact: Brenton Graveley, UConn

Summary

CRISPR RNA-seq of SMAD4 in Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens SMAD4

Overall Design

RNA-seq on HepG2 cells treated with a CRISPR gRNA against SMAD4. (SMAD4-BGHcLV18)

Analysis page is available for ENCSR700FWD.

ENCODE Metadata

Total file size: 56.7 GB
Assay: CRISPR RNA-seq
Target: SMAD4
Biosample: Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens SMAD4
Files: 22
Unique ENC file IDs: 22
File Formats
bigWig (8) tsv (6) bam (4) fastq (4)
Output types (9)
reads (4) transcript quantifications (4) alignments (2) gene quantifications (2) minus strand signal of all reads (2) minus strand signal of unique reads (2) plus strand signal of all reads (2) plus strand signal of unique reads (2) transcriptome alignments (2)

Data Files (22)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
ENCFF150PFW ENCFF150PFW.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 6.0 GB link
ENCFF155BSS ENCFF155BSS.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 7.0 GB link
ENCFF007DBQ ENCFF007DBQ.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 15.3 GB link
ENCFF314WUT ENCFF314WUT.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 13.2 GB link
ENCFF564GSG ENCFF564GSG.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 144.4 MB link
ENCFF567GGJ ENCFF567GGJ.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 154.2 MB link
ENCFF084QTU ENCFF084QTU.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 129.1 MB link
ENCFF856AYM ENCFF856AYM.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 138.1 MB link
ENCFF638FSY ENCFF638FSY.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 146.7 MB link
ENCFF881AHW ENCFF881AHW.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 156.7 MB link
ENCFF046COE ENCFF046COE.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 141.6 MB link
ENCFF781CVD ENCFF781CVD.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 132.3 MB link
ENCFF442SXF ENCFF442SXF.fastq fastq (reads) reads 3.7 GB link
ENCFF576JKC ENCFF576JKC.fastq fastq (reads) reads 3.3 GB link
ENCFF591YVS ENCFF591YVS.fastq fastq (reads) reads 3.8 GB link
ENCFF825JUA ENCFF825JUA.fastq fastq (reads) reads 3.2 GB link
ENCFF085UPS ENCFF085UPS.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.5 MB link
ENCFF113WMD ENCFF113WMD.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.5 MB link
ENCFF155FKJ ENCFF155FKJ.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.9 MB link
ENCFF231JJI ENCFF231JJI.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.5 MB link
ENCFF499GPI ENCFF499GPI.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.6 MB link
ENCFF620NQP ENCFF620NQP.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.9 MB link