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ENCSR175OSV

ENCSR ENCODE
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RNA-seq on HepG2 cells treated with a CRISPR gRNA against DDX39B. (DDX39B-BGHcLV18)

Organism: Homo sapiens
Experiment Types:
CRISPR RNA-seq
Status: released
Contact: Brenton Graveley, UConn

Summary

CRISPR RNA-seq of DDX39B in Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens DDX39B

Overall Design

RNA-seq on HepG2 cells treated with a CRISPR gRNA against DDX39B. (DDX39B-BGHcLV18)

Analysis page is available for ENCSR175OSV.

ENCODE Metadata

Total file size: 62.5 GB
Assay: CRISPR RNA-seq
Target: DDX39B
Biosample: Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens DDX39B
Files: 22
Unique ENC file IDs: 22
File Formats
bigWig (8) tsv (6) bam (4) fastq (4)
Output types (9)
reads (4) transcript quantifications (4) alignments (2) gene quantifications (2) minus strand signal of all reads (2) minus strand signal of unique reads (2) plus strand signal of all reads (2) plus strand signal of unique reads (2) transcriptome alignments (2)

Data Files (22)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
ENCFF266TDS ENCFF266TDS.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 7.1 GB link
ENCFF430IUX ENCFF430IUX.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 7.2 GB link
ENCFF295HHL ENCFF295HHL.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 15.4 GB link
ENCFF576LEB ENCFF576LEB.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 15.8 GB link
ENCFF646LPM ENCFF646LPM.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 158.4 MB link
ENCFF974IVJ ENCFF974IVJ.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 160.7 MB link
ENCFF437UPD ENCFF437UPD.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 143.2 MB link
ENCFF478LOA ENCFF478LOA.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 143.5 MB link
ENCFF205IQU ENCFF205IQU.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 160.7 MB link
ENCFF414HDC ENCFF414HDC.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 162.9 MB link
ENCFF152WUA ENCFF152WUA.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 146.9 MB link
ENCFF532QMR ENCFF532QMR.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 146.7 MB link
ENCFF065RTJ ENCFF065RTJ.fastq fastq (reads) reads 3.8 GB link
ENCFF265GAA ENCFF265GAA.fastq fastq (reads) reads 4.0 GB link
ENCFF587WWR ENCFF587WWR.fastq fastq (reads) reads 3.9 GB link
ENCFF742NWQ ENCFF742NWQ.fastq fastq (reads) reads 3.8 GB link
ENCFF506GAT ENCFF506GAT.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.5 MB link
ENCFF557CUA ENCFF557CUA.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.5 MB link
ENCFF321ULN ENCFF321ULN.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.6 MB link
ENCFF476XYU ENCFF476XYU.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.9 MB link
ENCFF638WOA ENCFF638WOA.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.9 MB link
ENCFF721LAE ENCFF721LAE.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.6 MB link