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ENCSR580XTY

ENCSR ENCODE
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RNA-seq on HepG2 cells treated with a CRISPR gRNA against PDCD4. (PDCD4-BGHcLV18)

Organism: Homo sapiens
Experiment Types:
CRISPR RNA-seq
Status: released
Contact: Brenton Graveley, UConn

Summary

CRISPR RNA-seq of PDCD4 in Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens PDCD4

Overall Design

RNA-seq on HepG2 cells treated with a CRISPR gRNA against PDCD4. (PDCD4-BGHcLV18)

Analysis page is available for ENCSR580XTY.

ENCODE Metadata

Total file size: 55.3 GB
Assay: CRISPR RNA-seq
Target: PDCD4
Biosample: Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens PDCD4
Files: 22
Unique ENC file IDs: 22
File Formats
bigWig (8) tsv (6) bam (4) fastq (4)
Output types (9)
reads (4) transcript quantifications (4) alignments (2) gene quantifications (2) minus strand signal of all reads (2) minus strand signal of unique reads (2) plus strand signal of all reads (2) plus strand signal of unique reads (2) transcriptome alignments (2)

Data Files (22)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
ENCFF144LEJ ENCFF144LEJ.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 6.0 GB link
ENCFF210VOQ ENCFF210VOQ.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 6.9 GB link
ENCFF213XXI ENCFF213XXI.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 14.9 GB link
ENCFF822FGJ ENCFF822FGJ.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 12.7 GB link
ENCFF625RRQ ENCFF625RRQ.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 140.8 MB link
ENCFF902IWP ENCFF902IWP.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 151.1 MB link
ENCFF231ZRP ENCFF231ZRP.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 136.7 MB link
ENCFF918NII ENCFF918NII.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 125.6 MB link
ENCFF352PWI ENCFF352PWI.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 153.1 MB link
ENCFF552BZD ENCFF552BZD.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 142.8 MB link
ENCFF086DDZ ENCFF086DDZ.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 139.9 MB link
ENCFF545QIM ENCFF545QIM.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 128.5 MB link
ENCFF045UGV ENCFF045UGV.fastq fastq (reads) reads 3.2 GB link
ENCFF455XWV ENCFF455XWV.fastq fastq (reads) reads 3.1 GB link
ENCFF693SYT ENCFF693SYT.fastq fastq (reads) reads 3.8 GB link
ENCFF854HZB ENCFF854HZB.fastq fastq (reads) reads 3.6 GB link
ENCFF451KFL ENCFF451KFL.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.5 MB link
ENCFF808QSH ENCFF808QSH.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.5 MB link
ENCFF076GUF ENCFF076GUF.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.9 MB link
ENCFF188DKV ENCFF188DKV.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.5 MB link
ENCFF465USK ENCFF465USK.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.9 MB link
ENCFF575NTQ ENCFF575NTQ.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.5 MB link