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ENCSR383QYT

ENCSR ENCODE
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RNA-seq on HepG2 cells treated with a CRISPR gRNA against SMAD5. (SMAD5-BGHcLV18)

Organism: Homo sapiens
Experiment Types:
CRISPR RNA-seq
Status: released
Contact: Brenton Graveley, UConn

Summary

CRISPR RNA-seq of SMAD5 in Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens SMAD5

Overall Design

RNA-seq on HepG2 cells treated with a CRISPR gRNA against SMAD5. (SMAD5-BGHcLV18)

Analysis page is available for ENCSR383QYT.

ENCODE Metadata

Total file size: 53.9 GB
Assay: CRISPR RNA-seq
Target: SMAD5
Biosample: Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens SMAD5
Files: 22
Unique ENC file IDs: 22
File Formats
bigWig (8) tsv (6) bam (4) fastq (4)
Output types (9)
reads (4) transcript quantifications (4) alignments (2) gene quantifications (2) minus strand signal of all reads (2) minus strand signal of unique reads (2) plus strand signal of all reads (2) plus strand signal of unique reads (2) transcriptome alignments (2)

Data Files (22)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
ENCFF950SMK ENCFF950SMK.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 6.5 GB link
ENCFF991NSN ENCFF991NSN.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 5.9 GB link
ENCFF233NNG ENCFF233NNG.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 14.1 GB link
ENCFF694CDB ENCFF694CDB.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 12.8 GB link
ENCFF671HAG ENCFF671HAG.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 149.0 MB link
ENCFF723DQN ENCFF723DQN.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 138.4 MB link
ENCFF484SVJ ENCFF484SVJ.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 124.8 MB link
ENCFF610GJZ ENCFF610GJZ.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 134.8 MB link
ENCFF281ARX ENCFF281ARX.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 151.2 MB link
ENCFF351OSA ENCFF351OSA.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 140.5 MB link
ENCFF693JTL ENCFF693JTL.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 138.0 MB link
ENCFF823FGZ ENCFF823FGZ.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 127.8 MB link
ENCFF284NLV ENCFF284NLV.fastq fastq (reads) reads 3.4 GB link
ENCFF319ANQ ENCFF319ANQ.fastq fastq (reads) reads 3.1 GB link
ENCFF474AIV ENCFF474AIV.fastq fastq (reads) reads 3.2 GB link
ENCFF513WXY ENCFF513WXY.fastq fastq (reads) reads 3.6 GB link
ENCFF634GCE ENCFF634GCE.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.5 MB link
ENCFF839CMB ENCFF839CMB.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.5 MB link
ENCFF005FYE ENCFF005FYE.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.6 MB link
ENCFF038DYX ENCFF038DYX.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.9 MB link
ENCFF045HSN ENCFF045HSN.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.5 MB link
ENCFF423NHJ ENCFF423NHJ.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.9 MB link