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ENCSR989GNV

ENCSR ENCODE
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RNA-seq on HepG2 cells treated with a CRISPR gRNA against SMYD3. (SMYD3-BGHcLV20)

Organism: Homo sapiens
Experiment Types:
CRISPR RNA-seq
Status: released
Contact: Brenton Graveley, UConn

Summary

CRISPR RNA-seq of SMYD3 in Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens SMYD3

Overall Design

RNA-seq on HepG2 cells treated with a CRISPR gRNA against SMYD3. (SMYD3-BGHcLV20)

Analysis page is available for ENCSR989GNV.

ENCODE Metadata

Total file size: 31.1 GB
Assay: CRISPR RNA-seq
Target: SMYD3
Biosample: Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens SMYD3
Files: 22
Unique ENC file IDs: 22
File Formats
bigWig (8) tsv (6) bam (4) fastq (4)
Output types (9)
reads (4) transcript quantifications (4) alignments (2) gene quantifications (2) minus strand signal of all reads (2) minus strand signal of unique reads (2) plus strand signal of all reads (2) plus strand signal of unique reads (2) transcriptome alignments (2)

Data Files (22)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
ENCFF356ZEB ENCFF356ZEB.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 3.4 GB link
ENCFF857HUP ENCFF857HUP.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 3.6 GB link
ENCFF731AEV ENCFF731AEV.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 7.3 GB link
ENCFF761HJU ENCFF761HJU.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 7.9 GB link
ENCFF121CMA ENCFF121CMA.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 90.2 MB link
ENCFF537LAE ENCFF537LAE.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 91.7 MB link
ENCFF435TYC ENCFF435TYC.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 80.2 MB link
ENCFF657HDF ENCFF657HDF.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 83.0 MB link
ENCFF081NON ENCFF081NON.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 91.8 MB link
ENCFF985ZPD ENCFF985ZPD.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 93.1 MB link
ENCFF178HVX ENCFF178HVX.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 85.0 MB link
ENCFF191HBP ENCFF191HBP.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 82.3 MB link
ENCFF101QIC ENCFF101QIC.fastq fastq (reads) reads 2.0 GB link
ENCFF612FHM ENCFF612FHM.fastq fastq (reads) reads 2.1 GB link
ENCFF666NLT ENCFF666NLT.fastq fastq (reads) reads 2.1 GB link
ENCFF867UNS ENCFF867UNS.fastq fastq (reads) reads 1.9 GB link
ENCFF561RVT ENCFF561RVT.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF750WPR ENCFF750WPR.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.5 MB link
ENCFF146EAA ENCFF146EAA.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.9 MB link
ENCFF260ETV ENCFF260ETV.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.4 MB link
ENCFF576SIQ ENCFF576SIQ.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.4 MB link
ENCFF709IWK ENCFF709IWK.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.9 MB link