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ENCSR528LDT

ENCSR ENCODE
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RNA-seq on HepG2 cells treated with a CRISPR gRNA against PTGES3. (PTGES3-BGHcLV16)

Organism: Homo sapiens
Experiment Types:
CRISPR RNA-seq
Status: released
Contact: Brenton Graveley, UConn

Summary

CRISPR RNA-seq of PTGES3 in Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens PTGES3

Overall Design

RNA-seq on HepG2 cells treated with a CRISPR gRNA against PTGES3. (PTGES3-BGHcLV16)

Analysis page is available for ENCSR528LDT.

ENCODE Metadata

Total file size: 44.5 GB
Assay: CRISPR RNA-seq
Target: PTGES3
Biosample: Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens PTGES3
Files: 22
Unique ENC file IDs: 22
File Formats
bigWig (8) tsv (6) bam (4) fastq (4)
Output types (9)
reads (4) transcript quantifications (4) alignments (2) gene quantifications (2) minus strand signal of all reads (2) minus strand signal of unique reads (2) plus strand signal of all reads (2) plus strand signal of unique reads (2) transcriptome alignments (2)

Data Files (22)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
ENCFF089UDE ENCFF089UDE.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 5.4 GB link
ENCFF530WKS ENCFF530WKS.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 5.1 GB link
ENCFF648SZY ENCFF648SZY.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 10.6 GB link
ENCFF648YBE ENCFF648YBE.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 11.3 GB link
ENCFF268GOC ENCFF268GOC.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 125.6 MB link
ENCFF530NMU ENCFF530NMU.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 127.7 MB link
ENCFF503DCB ENCFF503DCB.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 115.0 MB link
ENCFF556HZX ENCFF556HZX.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 112.1 MB link
ENCFF197YQE ENCFF197YQE.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 129.8 MB link
ENCFF287QJR ENCFF287QJR.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 127.9 MB link
ENCFF294LWP ENCFF294LWP.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 117.8 MB link
ENCFF403RRJ ENCFF403RRJ.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 115.2 MB link
ENCFF223HNZ ENCFF223HNZ.fastq fastq (reads) reads 2.6 GB link
ENCFF410AVA ENCFF410AVA.fastq fastq (reads) reads 2.8 GB link
ENCFF503ZFN ENCFF503ZFN.fastq fastq (reads) reads 2.8 GB link
ENCFF959NTL ENCFF959NTL.fastq fastq (reads) reads 2.9 GB link
ENCFF044YCF ENCFF044YCF.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF221QJB ENCFF221QJB.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF087NTM ENCFF087NTM.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.9 MB link
ENCFF785ANN ENCFF785ANN.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.5 MB link
ENCFF796HSO ENCFF796HSO.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.5 MB link
ENCFF914VTY ENCFF914VTY.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.9 MB link