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ENCSR013PBS

ENCSR ENCODE
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RNA-seq on HepG2 cells treated with a CRISPR gRNA against NCL. (NCL-BGHcLV18)

Organism: Homo sapiens
Experiment Types:
CRISPR RNA-seq
Status: released
Contact: Brenton Graveley, UConn

Summary

CRISPR RNA-seq of NCL in Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens NCL

Overall Design

RNA-seq on HepG2 cells treated with a CRISPR gRNA against NCL. (NCL-BGHcLV18)

Analysis page is available for ENCSR013PBS.

ENCODE Metadata

Total file size: 68.2 GB
Assay: CRISPR RNA-seq
Target: NCL
Biosample: Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens NCL
Files: 22
Unique ENC file IDs: 22
File Formats
bigWig (8) tsv (6) bam (4) fastq (4)
Output types (9)
reads (4) transcript quantifications (4) alignments (2) gene quantifications (2) minus strand signal of all reads (2) minus strand signal of unique reads (2) plus strand signal of all reads (2) plus strand signal of unique reads (2) transcriptome alignments (2)

Data Files (22)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
ENCFF324TLX ENCFF324TLX.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 6.2 GB link
ENCFF858ZTF ENCFF858ZTF.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 9.5 GB link
ENCFF209MCW ENCFF209MCW.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 21.2 GB link
ENCFF446XNB ENCFF446XNB.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 13.0 GB link
ENCFF341SRS ENCFF341SRS.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 140.0 MB link
ENCFF407DJW ENCFF407DJW.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 176.3 MB link
ENCFF011ZUI ENCFF011ZUI.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 125.5 MB link
ENCFF935JPI ENCFF935JPI.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 157.8 MB link
ENCFF109AUA ENCFF109AUA.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 142.1 MB link
ENCFF613XKD ENCFF613XKD.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 178.8 MB link
ENCFF072DAL ENCFF072DAL.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 161.5 MB link
ENCFF323WTI ENCFF323WTI.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 128.5 MB link
ENCFF453FLY ENCFF453FLY.fastq fastq (reads) reads 5.1 GB link
ENCFF649ODX ENCFF649ODX.fastq fastq (reads) reads 3.4 GB link
ENCFF692NAO ENCFF692NAO.fastq fastq (reads) reads 3.2 GB link
ENCFF784SYJ ENCFF784SYJ.fastq fastq (reads) reads 5.3 GB link
ENCFF145ORB ENCFF145ORB.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.5 MB link
ENCFF705THZ ENCFF705THZ.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.5 MB link
ENCFF018QCA ENCFF018QCA.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.9 MB link
ENCFF355DSV ENCFF355DSV.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 30.0 MB link
ENCFF611TVY ENCFF611TVY.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.5 MB link
ENCFF639HPB ENCFF639HPB.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.6 MB link