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ENCSR036ANR

ENCSR ENCODE
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RNA-seq on HepG2 cells treated with a CRISPR gRNA against SMAD2. (SMAD2-BGHcLV16)

Organism: Homo sapiens
Experiment Types:
CRISPR RNA-seq
Status: released
Contact: Brenton Graveley, UConn

Summary

CRISPR RNA-seq of SMAD2 in Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens SMAD2

Overall Design

RNA-seq on HepG2 cells treated with a CRISPR gRNA against SMAD2. (SMAD2-BGHcLV16)

Analysis page is available for ENCSR036ANR.

ENCODE Metadata

Total file size: 57.4 GB
Assay: CRISPR RNA-seq
Target: SMAD2
Biosample: Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens SMAD2
Files: 22
Unique ENC file IDs: 22
File Formats
bigWig (8) tsv (6) bam (4) fastq (4)
Output types (9)
reads (4) transcript quantifications (4) alignments (2) gene quantifications (2) minus strand signal of all reads (2) minus strand signal of unique reads (2) plus strand signal of all reads (2) plus strand signal of unique reads (2) transcriptome alignments (2)

Data Files (22)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
ENCFF131GEG ENCFF131GEG.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 4.2 GB link
ENCFF228FZZ ENCFF228FZZ.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 9.2 GB link
ENCFF045TKA ENCFF045TKA.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 9.2 GB link
ENCFF263PNC ENCFF263PNC.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 19.5 GB link
ENCFF177PFY ENCFF177PFY.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 147.7 MB link
ENCFF914FOB ENCFF914FOB.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 105.5 MB link
ENCFF048IHE ENCFF048IHE.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 134.6 MB link
ENCFF740XQT ENCFF740XQT.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 94.2 MB link
ENCFF387KVI ENCFF387KVI.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 150.3 MB link
ENCFF776OQP ENCFF776OQP.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 107.7 MB link
ENCFF639COX ENCFF639COX.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 96.9 MB link
ENCFF657JCH ENCFF657JCH.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 138.0 MB link
ENCFF222WVJ ENCFF222WVJ.fastq fastq (reads) reads 5.0 GB link
ENCFF518ZFP ENCFF518ZFP.fastq fastq (reads) reads 4.7 GB link
ENCFF620DRO ENCFF620DRO.fastq fastq (reads) reads 2.3 GB link
ENCFF951XMO ENCFF951XMO.fastq fastq (reads) reads 2.2 GB link
ENCFF382KDR ENCFF382KDR.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.5 MB link
ENCFF760IAA ENCFF760IAA.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF026JOG ENCFF026JOG.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 30.0 MB link
ENCFF222QND ENCFF222QND.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.4 MB link
ENCFF505ILS ENCFF505ILS.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.6 MB link
ENCFF763RST ENCFF763RST.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.9 MB link