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ENCSR665DMA

ENCSR ENCODE
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RNA-seq on K562 cells treated with a CRISPR gRNA against MECP2. (MECP2-BGKcLV15)

Organism: Homo sapiens
Experiment Types:
CRISPR RNA-seq
Status: released
Contact: Brenton Graveley, UConn

Summary

CRISPR RNA-seq of MECP2 in Homo sapiens K562 genetically modified (deletion) using CRISPR targeting H. sapiens MECP2

Overall Design

RNA-seq on K562 cells treated with a CRISPR gRNA against MECP2. (MECP2-BGKcLV15)

Analysis page is available for ENCSR665DMA.

ENCODE Metadata

Total file size: 36.9 GB
Assay: CRISPR RNA-seq
Target: MECP2
Biosample: Homo sapiens K562 genetically modified (deletion) using CRISPR targeting H. sapiens MECP2
Files: 22
Unique ENC file IDs: 22
File Formats
bigWig (8) tsv (6) bam (4) fastq (4)
Output types (9)
reads (4) transcript quantifications (4) alignments (2) gene quantifications (2) minus strand signal of all reads (2) minus strand signal of unique reads (2) plus strand signal of all reads (2) plus strand signal of unique reads (2) transcriptome alignments (2)

Data Files (22)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
ENCFF098HAN ENCFF098HAN.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 3.9 GB link
ENCFF881JZV ENCFF881JZV.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 5.1 GB link
ENCFF910ZYC ENCFF910ZYC.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 7.5 GB link
ENCFF980IBE ENCFF980IBE.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 9.9 GB link
ENCFF357XVR ENCFF357XVR.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 133.9 MB link
ENCFF924KBB ENCFF924KBB.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 144.0 MB link
ENCFF351IPE ENCFF351IPE.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 126.4 MB link
ENCFF850BST ENCFF850BST.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 116.9 MB link
ENCFF859UQG ENCFF859UQG.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 146.3 MB link
ENCFF915OWX ENCFF915OWX.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 136.0 MB link
ENCFF365ZLP ENCFF365ZLP.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 129.7 MB link
ENCFF394HHQ ENCFF394HHQ.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 120.0 MB link
ENCFF135VUI ENCFF135VUI.fastq fastq (reads) reads 2.6 GB link
ENCFF201MYS ENCFF201MYS.fastq fastq (reads) reads 2.1 GB link
ENCFF612ORU ENCFF612ORU.fastq fastq (reads) reads 2.8 GB link
ENCFF831TST ENCFF831TST.fastq fastq (reads) reads 2.0 GB link
ENCFF499GXV ENCFF499GXV.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF705LXM ENCFF705LXM.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.5 MB link
ENCFF632HFG ENCFF632HFG.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.5 MB link
ENCFF664BAS ENCFF664BAS.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.9 MB link
ENCFF725UGZ ENCFF725UGZ.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.5 MB link
ENCFF945DPU ENCFF945DPU.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.9 MB link