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ENCSR999MAE

ENCSR ENCODE
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RNA-seq on HepG2 cells treated with a CRISPR gRNA against EIF4A3. (EIF4A3-BGHcLV16)

Organism: Homo sapiens
Experiment Types:
CRISPR RNA-seq
Status: released
Contact: Brenton Graveley, UConn

Summary

CRISPR RNA-seq of EIF4A3 in Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens EIF4A3

Overall Design

RNA-seq on HepG2 cells treated with a CRISPR gRNA against EIF4A3. (EIF4A3-BGHcLV16)

Analysis

View Data Processing
Analysis page is available for ENCSR999MAE.

ENCODE Metadata

Total file size: 39.8 GB
Assay: CRISPR RNA-seq
Target: EIF4A3
Biosample: Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens EIF4A3
Files: 22
Unique ENC file IDs: 22
File Formats
bigWig (8) tsv (6) bam (4) fastq (4)
Output types (9)
reads (4) transcript quantifications (4) alignments (2) gene quantifications (2) minus strand signal of all reads (2) minus strand signal of unique reads (2) plus strand signal of all reads (2) plus strand signal of unique reads (2) transcriptome alignments (2)

Data Files (22)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
ENCFF181FNQ ENCFF181FNQ.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 5.0 GB link
ENCFF650AQR ENCFF650AQR.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 4.6 GB link
ENCFF763MHK ENCFF763MHK.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 9.1 GB link
ENCFF851MVB ENCFF851MVB.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 10.0 GB link
ENCFF012EFP ENCFF012EFP.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 130.5 MB link
ENCFF490BKT ENCFF490BKT.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 124.1 MB link
ENCFF091NEE ENCFF091NEE.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 117.2 MB link
ENCFF782IZC ENCFF782IZC.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 111.4 MB link
ENCFF292DNE ENCFF292DNE.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 132.9 MB link
ENCFF884HST ENCFF884HST.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 126.3 MB link
ENCFF688WWX ENCFF688WWX.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 114.3 MB link
ENCFF917USO ENCFF917USO.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 120.2 MB link
ENCFF012VVL ENCFF012VVL.fastq fastq (reads) reads 2.5 GB link
ENCFF782UNR ENCFF782UNR.fastq fastq (reads) reads 2.7 GB link
ENCFF790UHH ENCFF790UHH.fastq fastq (reads) reads 2.5 GB link
ENCFF818ONJ ENCFF818ONJ.fastq fastq (reads) reads 2.3 GB link
ENCFF779ZIT ENCFF779ZIT.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF811CJG ENCFF811CJG.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF172KZG ENCFF172KZG.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.9 MB link
ENCFF773TSZ ENCFF773TSZ.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.5 MB link
ENCFF783OWK ENCFF783OWK.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.5 MB link
ENCFF878QNJ ENCFF878QNJ.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.8 MB link