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ENCSR400XYB

ENCSR ENCODE
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RNA-seq on HepG2 cells treated with a CRISPR gRNA against CD3EAP. (CD3EAP-BGHcLV16)

Organism: Homo sapiens
Experiment Types:
CRISPR RNA-seq
Status: released
Contact: Brenton Graveley, UConn

Summary

CRISPR RNA-seq of CD3EAP in Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens CD3EAP

Overall Design

RNA-seq on HepG2 cells treated with a CRISPR gRNA against CD3EAP. (CD3EAP-BGHcLV16)

Analysis page is available for ENCSR400XYB.

ENCODE Metadata

Total file size: 42.4 GB
Assay: CRISPR RNA-seq
Target: CD3EAP
Biosample: Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens CD3EAP
Files: 22
Unique ENC file IDs: 22
File Formats
bigWig (8) tsv (6) bam (4) fastq (4)
Output types (9)
reads (4) transcript quantifications (4) alignments (2) gene quantifications (2) minus strand signal of all reads (2) minus strand signal of unique reads (2) plus strand signal of all reads (2) plus strand signal of unique reads (2) transcriptome alignments (2)

Data Files (22)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
ENCFF515EJP ENCFF515EJP.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 4.9 GB link
ENCFF608RKO ENCFF608RKO.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 5.1 GB link
ENCFF714FPQ ENCFF714FPQ.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 10.6 GB link
ENCFF725GIO ENCFF725GIO.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 10.2 GB link
ENCFF449PWP ENCFF449PWP.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 127.7 MB link
ENCFF592DFV ENCFF592DFV.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 127.5 MB link
ENCFF444CPH ENCFF444CPH.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 114.8 MB link
ENCFF501EFC ENCFF501EFC.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 114.2 MB link
ENCFF536OSH ENCFF536OSH.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 129.9 MB link
ENCFF667JZS ENCFF667JZS.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 129.6 MB link
ENCFF633WYA ENCFF633WYA.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 117.1 MB link
ENCFF660QUL ENCFF660QUL.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 117.8 MB link
ENCFF112QUX ENCFF112QUX.fastq fastq (reads) reads 2.6 GB link
ENCFF346QAG ENCFF346QAG.fastq fastq (reads) reads 2.7 GB link
ENCFF459ILB ENCFF459ILB.fastq fastq (reads) reads 2.8 GB link
ENCFF515GRY ENCFF515GRY.fastq fastq (reads) reads 2.5 GB link
ENCFF227AUT ENCFF227AUT.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.5 MB link
ENCFF728QQB ENCFF728QQB.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.5 MB link
ENCFF028MSM ENCFF028MSM.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.9 MB link
ENCFF500LYK ENCFF500LYK.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.5 MB link
ENCFF584TMZ ENCFF584TMZ.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.9 MB link
ENCFF879EVC ENCFF879EVC.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.5 MB link