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ENCSR266XKN

ENCSR ENCODE
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RNA-seq on HepG2 cells treated with a CRISPR gRNA against MECP2. (MECP2-BGHcLV16)

Organism: Homo sapiens
Experiment Types:
CRISPR RNA-seq
Status: released
Contact: Brenton Graveley, UConn

Summary

CRISPR RNA-seq of MECP2 in Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens MECP2

Overall Design

RNA-seq on HepG2 cells treated with a CRISPR gRNA against MECP2. (MECP2-BGHcLV16)

Analysis page is available for ENCSR266XKN.

ENCODE Metadata

Total file size: 52.3 GB
Assay: CRISPR RNA-seq
Target: MECP2
Biosample: Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens MECP2
Files: 22
Unique ENC file IDs: 22
File Formats
bigWig (8) tsv (6) bam (4) fastq (4)
Output types (9)
reads (4) transcript quantifications (4) alignments (2) gene quantifications (2) minus strand signal of all reads (2) minus strand signal of unique reads (2) plus strand signal of all reads (2) plus strand signal of unique reads (2) transcriptome alignments (2)

Data Files (22)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
ENCFF791SRA ENCFF791SRA.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 6.1 GB link
ENCFF986BWP ENCFF986BWP.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 6.2 GB link
ENCFF282TQX ENCFF282TQX.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 12.7 GB link
ENCFF493VMR ENCFF493VMR.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 13.0 GB link
ENCFF049NZL ENCFF049NZL.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 143.8 MB link
ENCFF131SZN ENCFF131SZN.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 140.7 MB link
ENCFF229DEG ENCFF229DEG.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 126.7 MB link
ENCFF507UMK ENCFF507UMK.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 127.3 MB link
ENCFF637PNJ ENCFF637PNJ.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 143.3 MB link
ENCFF658SDO ENCFF658SDO.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 146.5 MB link
ENCFF334XEX ENCFF334XEX.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 130.1 MB link
ENCFF995AWM ENCFF995AWM.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 130.9 MB link
ENCFF616IUR ENCFF616IUR.fastq fastq (reads) reads 3.4 GB link
ENCFF708AZO ENCFF708AZO.fastq fastq (reads) reads 3.4 GB link
ENCFF890FAL ENCFF890FAL.fastq fastq (reads) reads 3.2 GB link
ENCFF999KCZ ENCFF999KCZ.fastq fastq (reads) reads 3.2 GB link
ENCFF540DXH ENCFF540DXH.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.5 MB link
ENCFF566TNU ENCFF566TNU.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.5 MB link
ENCFF089KNP ENCFF089KNP.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.3 MB link
ENCFF104SPX ENCFF104SPX.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.6 MB link
ENCFF172HDY ENCFF172HDY.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.9 MB link
ENCFF569DIN ENCFF569DIN.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.9 MB link