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ENCSR470SBK

ENCSR ENCODE
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RNA-seq on HepG2 cells treated with a CRISPR gRNA against CDK9. (CDK9-BGHcLV29)

Organism: Homo sapiens
Experiment Types:
CRISPR RNA-seq
Status: released
Contact: Brenton Graveley, UConn

Summary

CRISPR RNA-seq of CDK9 in Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens CDK9

Overall Design

RNA-seq on HepG2 cells treated with a CRISPR gRNA against CDK9. (CDK9-BGHcLV29)

Analysis page is available for ENCSR470SBK.

ENCODE Metadata

Total file size: 22.4 GB
Assay: CRISPR RNA-seq
Target: CDK9
Biosample: Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens CDK9
Files: 22
Unique ENC file IDs: 22
File Formats
bigWig (8) tsv (6) bam (4) fastq (4)
Output types (9)
reads (4) transcript quantifications (4) alignments (2) gene quantifications (2) minus strand signal of all reads (2) minus strand signal of unique reads (2) plus strand signal of all reads (2) plus strand signal of unique reads (2) transcriptome alignments (2)

Data Files (22)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
ENCFF661UHN ENCFF661UHN.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 2.5 GB link
ENCFF687SCR ENCFF687SCR.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 3.0 GB link
ENCFF728QSI ENCFF728QSI.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 4.9 GB link
ENCFF849SFV ENCFF849SFV.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 5.8 GB link
ENCFF647YIO ENCFF647YIO.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 77.7 MB link
ENCFF833HED ENCFF833HED.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 85.7 MB link
ENCFF222DFG ENCFF222DFG.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 77.7 MB link
ENCFF362JSP ENCFF362JSP.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 69.9 MB link
ENCFF107XEZ ENCFF107XEZ.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 78.9 MB link
ENCFF604RZL ENCFF604RZL.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 87.0 MB link
ENCFF258IAM ENCFF258IAM.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 79.4 MB link
ENCFF678SPY ENCFF678SPY.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 71.4 MB link
ENCFF002HWI ENCFF002HWI.fastq fastq (reads) reads 1.2 GB link
ENCFF478DHP ENCFF478DHP.fastq fastq (reads) reads 1.3 GB link
ENCFF518WNH ENCFF518WNH.fastq fastq (reads) reads 1.5 GB link
ENCFF855KOE ENCFF855KOE.fastq fastq (reads) reads 1.5 GB link
ENCFF104ZYU ENCFF104ZYU.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF941RFG ENCFF941RFG.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF058NSP ENCFF058NSP.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.8 MB link
ENCFF063QRW ENCFF063QRW.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.8 MB link
ENCFF615IFG ENCFF615IFG.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.3 MB link
ENCFF845QWC ENCFF845QWC.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.3 MB link