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ENCSR034VEC

ENCSR ENCODE
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RNA-seq on HepG2 cells treated with a CRISPR gRNA against ROCK2. (ROCK2-BGHcLV32)

Organism: Homo sapiens
Experiment Types:
CRISPR RNA-seq
Status: released
Contact: Brenton Graveley, UConn

Summary

CRISPR RNA-seq of ROCK2 in Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens ROCK2

Overall Design

RNA-seq on HepG2 cells treated with a CRISPR gRNA against ROCK2. (ROCK2-BGHcLV32)

Analysis page is available for ENCSR034VEC.

ENCODE Metadata

Total file size: 25.5 GB
Assay: CRISPR RNA-seq
Target: ROCK2
Biosample: Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens ROCK2
Files: 22
Unique ENC file IDs: 22
File Formats
bigWig (8) tsv (6) bam (4) fastq (4)
Output types (9)
reads (4) transcript quantifications (4) alignments (2) gene quantifications (2) minus strand signal of all reads (2) minus strand signal of unique reads (2) plus strand signal of all reads (2) plus strand signal of unique reads (2) transcriptome alignments (2)

Data Files (22)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
ENCFF579XCT ENCFF579XCT.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 3.6 GB link
ENCFF664VSF ENCFF664VSF.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 2.8 GB link
ENCFF376NNQ ENCFF376NNQ.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 6.7 GB link
ENCFF400EIW ENCFF400EIW.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 5.1 GB link
ENCFF749PKY ENCFF749PKY.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 70.0 MB link
ENCFF976PDC ENCFF976PDC.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 80.1 MB link
ENCFF697VPQ ENCFF697VPQ.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 71.7 MB link
ENCFF862CFC ENCFF862CFC.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 63.2 MB link
ENCFF181UBA ENCFF181UBA.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 81.0 MB link
ENCFF657LHW ENCFF657LHW.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 70.9 MB link
ENCFF208SUC ENCFF208SUC.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 64.6 MB link
ENCFF218IQX ENCFF218IQX.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 73.1 MB link
ENCFF013UMZ ENCFF013UMZ.fastq fastq (reads) reads 1.4 GB link
ENCFF639EZB ENCFF639EZB.fastq fastq (reads) reads 1.9 GB link
ENCFF809PBA ENCFF809PBA.fastq fastq (reads) reads 1.5 GB link
ENCFF877QZL ENCFF877QZL.fastq fastq (reads) reads 1.8 GB link
ENCFF477OXQ ENCFF477OXQ.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF937JNP ENCFF937JNP.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF208CAH ENCFF208CAH.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.9 MB link
ENCFF220UOD ENCFF220UOD.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.8 MB link
ENCFF436ZMO ENCFF436ZMO.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.3 MB link
ENCFF517PYS ENCFF517PYS.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.3 MB link