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ENCSR574ZTO

ENCSR ENCODE
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RNA-seq on HepG2 cells treated with a CRISPR gRNA against DARS. (DARS-BGHcLV29)

Organism: Homo sapiens
Experiment Types:
CRISPR RNA-seq
Status: released
Contact: Brenton Graveley, UConn

Summary

CRISPR RNA-seq of DARS in Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens DARS

Overall Design

RNA-seq on HepG2 cells treated with a CRISPR gRNA against DARS. (DARS-BGHcLV29)

Analysis page is available for ENCSR574ZTO.

ENCODE Metadata

Total file size: 31.5 GB
Assay: CRISPR RNA-seq
Target: DARS
Biosample: Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens DARS
Files: 22
Unique ENC file IDs: 22
File Formats
bigWig (8) tsv (6) bam (4) fastq (4)
Output types (9)
reads (4) transcript quantifications (4) alignments (2) gene quantifications (2) minus strand signal of all reads (2) minus strand signal of unique reads (2) plus strand signal of all reads (2) plus strand signal of unique reads (2) transcriptome alignments (2)

Data Files (22)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
ENCFF171VVY ENCFF171VVY.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 3.5 GB link
ENCFF717WIB ENCFF717WIB.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 4.0 GB link
ENCFF570XWI ENCFF570XWI.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 7.2 GB link
ENCFF639KCL ENCFF639KCL.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 8.2 GB link
ENCFF033IZF ENCFF033IZF.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 102.0 MB link
ENCFF039TPZ ENCFF039TPZ.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 88.2 MB link
ENCFF398NIN ENCFF398NIN.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 80.3 MB link
ENCFF543KFC ENCFF543KFC.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 91.5 MB link
ENCFF742XTP ENCFF742XTP.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 103.5 MB link
ENCFF844HIH ENCFF844HIH.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 89.6 MB link
ENCFF090SJF ENCFF090SJF.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 82.1 MB link
ENCFF563WMX ENCFF563WMX.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 93.7 MB link
ENCFF108BLS ENCFF108BLS.fastq fastq (reads) reads 1.8 GB link
ENCFF367TUN ENCFF367TUN.fastq fastq (reads) reads 1.8 GB link
ENCFF389XFB ENCFF389XFB.fastq fastq (reads) reads 2.1 GB link
ENCFF517ILP ENCFF517ILP.fastq fastq (reads) reads 2.0 GB link
ENCFF747JSQ ENCFF747JSQ.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF978KWT ENCFF978KWT.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF142KSR ENCFF142KSR.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.8 MB link
ENCFF180GHG ENCFF180GHG.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.4 MB link
ENCFF449UGO ENCFF449UGO.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.4 MB link
ENCFF555UMH ENCFF555UMH.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.8 MB link