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ENCSR029LLJ

ENCSR ENCODE
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RNA-seq on HepG2 cells treated with a CRISPR gRNA against ACTN4. (ACTN4-BGHcLV25)

Organism: Homo sapiens
Experiment Types:
CRISPR RNA-seq
Status: released
Contact: Brenton Graveley, UConn

Summary

CRISPR RNA-seq of ACTN4 in Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens ACTN4

Overall Design

RNA-seq on HepG2 cells treated with a CRISPR gRNA against ACTN4. (ACTN4-BGHcLV25)

Analysis page is available for ENCSR029LLJ.

ENCODE Metadata

Total file size: 37.3 GB
Assay: CRISPR RNA-seq
Target: ACTN4
Biosample: Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens ACTN4
Files: 22
Unique ENC file IDs: 22
File Formats
bigWig (8) tsv (6) bam (4) fastq (4)
Output types (9)
reads (4) transcript quantifications (4) alignments (2) gene quantifications (2) minus strand signal of all reads (2) minus strand signal of unique reads (2) plus strand signal of all reads (2) plus strand signal of unique reads (2) transcriptome alignments (2)

Data Files (22)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
ENCFF308ISK ENCFF308ISK.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 4.2 GB link
ENCFF863LFB ENCFF863LFB.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 4.6 GB link
ENCFF918VZX ENCFF918VZX.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 8.7 GB link
ENCFF921HMT ENCFF921HMT.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 9.6 GB link
ENCFF094PXD ENCFF094PXD.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 121.4 MB link
ENCFF684ZMW ENCFF684ZMW.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 115.8 MB link
ENCFF668FAW ENCFF668FAW.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 105.2 MB link
ENCFF673QZA ENCFF673QZA.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 109.8 MB link
ENCFF098PUL ENCFF098PUL.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 123.0 MB link
ENCFF511FQD ENCFF511FQD.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 117.3 MB link
ENCFF645ARJ ENCFF645ARJ.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 107.6 MB link
ENCFF847AQV ENCFF847AQV.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 112.3 MB link
ENCFF310XWV ENCFF310XWV.fastq fastq (reads) reads 2.4 GB link
ENCFF517SIN ENCFF517SIN.fastq fastq (reads) reads 2.5 GB link
ENCFF742EYW ENCFF742EYW.fastq fastq (reads) reads 2.2 GB link
ENCFF922SDP ENCFF922SDP.fastq fastq (reads) reads 2.3 GB link
ENCFF375QQZ ENCFF375QQZ.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF406CRY ENCFF406CRY.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF141NXX ENCFF141NXX.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.2 MB link
ENCFF469UAM ENCFF469UAM.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.9 MB link
ENCFF851MUB ENCFF851MUB.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.8 MB link
ENCFF886KTA ENCFF886KTA.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.5 MB link