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ENCSR312UYN

ENCSR ENCODE
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RNA-seq on HepG2 cells treated with a CRISPR gRNA against EIF2B3. (EIF2B3-BGHcLV16)

Organism: Homo sapiens
Experiment Types:
CRISPR RNA-seq
Status: released
Contact: Brenton Graveley, UConn

Summary

CRISPR RNA-seq of EIF2B3 in Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens EIF2B3

Overall Design

RNA-seq on HepG2 cells treated with a CRISPR gRNA against EIF2B3. (EIF2B3-BGHcLV16)

Analysis

View Data Processing
Analysis page is available for ENCSR312UYN.

ENCODE Metadata

Total file size: 37.4 GB
Assay: CRISPR RNA-seq
Target: EIF2B3
Biosample: Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens EIF2B3
Files: 22
Unique ENC file IDs: 22
File Formats
bigWig (8) tsv (6) bam (4) fastq (4)
Output types (9)
reads (4) transcript quantifications (4) alignments (2) gene quantifications (2) minus strand signal of all reads (2) minus strand signal of unique reads (2) plus strand signal of all reads (2) plus strand signal of unique reads (2) transcriptome alignments (2)

Data Files (22)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
ENCFF332WBZ ENCFF332WBZ.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 4.6 GB link
ENCFF342WDW ENCFF342WDW.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 4.1 GB link
ENCFF041DBR ENCFF041DBR.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 9.7 GB link
ENCFF468NCV ENCFF468NCV.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 8.7 GB link
ENCFF175PIV ENCFF175PIV.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 126.4 MB link
ENCFF281ROV ENCFF281ROV.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 112.5 MB link
ENCFF539WOG ENCFF539WOG.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 101.7 MB link
ENCFF771WAK ENCFF771WAK.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 112.2 MB link
ENCFF082VTQ ENCFF082VTQ.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 128.7 MB link
ENCFF726WCB ENCFF726WCB.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 114.5 MB link
ENCFF142AJH ENCFF142AJH.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 104.3 MB link
ENCFF711IWY ENCFF711IWY.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 115.2 MB link
ENCFF585CVR ENCFF585CVR.fastq fastq (reads) reads 2.1 GB link
ENCFF748HJK ENCFF748HJK.fastq fastq (reads) reads 2.5 GB link
ENCFF969QJR ENCFF969QJR.fastq fastq (reads) reads 2.2 GB link
ENCFF995FKT ENCFF995FKT.fastq fastq (reads) reads 2.4 GB link
ENCFF304YYU ENCFF304YYU.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF626UWS ENCFF626UWS.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF015LYK ENCFF015LYK.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.9 MB link
ENCFF050QRD ENCFF050QRD.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.4 MB link
ENCFF812SLJ ENCFF812SLJ.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.5 MB link
ENCFF830XRN ENCFF830XRN.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.9 MB link