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ENCSR025XBH

ENCSR ENCODE
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RNA-seq on K562 cells treated with a CRISPR gRNA against GSK3B. (GSK3B-BGKcLV15)

Organism: Homo sapiens
Experiment Types:
CRISPR RNA-seq
Status: released
Contact: Brenton Graveley, UConn

Summary

CRISPR RNA-seq of GSK3B in Homo sapiens K562 genetically modified (deletion) using CRISPR targeting H. sapiens GSK3B

Overall Design

RNA-seq on K562 cells treated with a CRISPR gRNA against GSK3B. (GSK3B-BGKcLV15)

Analysis page is available for ENCSR025XBH.

ENCODE Metadata

Total file size: 48.6 GB
Assay: CRISPR RNA-seq
Target: GSK3B
Biosample: Homo sapiens K562 genetically modified (deletion) using CRISPR targeting H. sapiens GSK3B
Files: 22
Unique ENC file IDs: 22
File Formats
bigWig (8) tsv (6) bam (4) fastq (4)
Output types (9)
reads (4) transcript quantifications (4) alignments (2) gene quantifications (2) minus strand signal of all reads (2) minus strand signal of unique reads (2) plus strand signal of all reads (2) plus strand signal of unique reads (2) transcriptome alignments (2)

Data Files (22)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
ENCFF487BJC ENCFF487BJC.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 8.4 GB link
ENCFF722QUU ENCFF722QUU.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 4.6 GB link
ENCFF368SEM ENCFF368SEM.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 13.1 GB link
ENCFF800JJQ ENCFF800JJQ.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 8.5 GB link
ENCFF421LPC ENCFF421LPC.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 138.5 MB link
ENCFF692ZEG ENCFF692ZEG.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 129.7 MB link
ENCFF594HPR ENCFF594HPR.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 114.3 MB link
ENCFF687OSG ENCFF687OSG.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 122.2 MB link
ENCFF228PJS ENCFF228PJS.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 140.5 MB link
ENCFF848GUA ENCFF848GUA.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 131.2 MB link
ENCFF522IGT ENCFF522IGT.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 116.8 MB link
ENCFF579AEM ENCFF579AEM.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 125.2 MB link
ENCFF305UXK ENCFF305UXK.fastq fastq (reads) reads 2.3 GB link
ENCFF517HJT ENCFF517HJT.fastq fastq (reads) reads 4.2 GB link
ENCFF676QUJ ENCFF676QUJ.fastq fastq (reads) reads 2.5 GB link
ENCFF805EIS ENCFF805EIS.fastq fastq (reads) reads 3.8 GB link
ENCFF077JDD ENCFF077JDD.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF435KHS ENCFF435KHS.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.5 MB link
ENCFF002RTS ENCFF002RTS.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.5 MB link
ENCFF304SGV ENCFF304SGV.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.9 MB link
ENCFF727PVD ENCFF727PVD.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.9 MB link
ENCFF788GHB ENCFF788GHB.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.4 MB link