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ENCSR870TGO

ENCSR ENCODE
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RNA-seq on K562 cells treated with a CRISPR gRNA against ACTN4. (ACTN4-BGKcLV26)

Organism: Homo sapiens
Experiment Types:
CRISPR RNA-seq
Status: released
Contact: Brenton Graveley, UConn

Summary

CRISPR RNA-seq of ACTN4 in Homo sapiens K562 genetically modified (deletion) using CRISPR targeting H. sapiens ACTN4

Overall Design

RNA-seq on K562 cells treated with a CRISPR gRNA against ACTN4. (ACTN4-BGKcLV26)

Analysis page is available for ENCSR870TGO.

ENCODE Metadata

Total file size: 35.2 GB
Assay: CRISPR RNA-seq
Target: ACTN4
Biosample: Homo sapiens K562 genetically modified (deletion) using CRISPR targeting H. sapiens ACTN4
Files: 22
Unique ENC file IDs: 22
File Formats
bigWig (8) tsv (6) bam (4) fastq (4)
Output types (9)
reads (4) transcript quantifications (4) alignments (2) gene quantifications (2) minus strand signal of all reads (2) minus strand signal of unique reads (2) plus strand signal of all reads (2) plus strand signal of unique reads (2) transcriptome alignments (2)

Data Files (22)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
ENCFF690VDT ENCFF690VDT.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 3.8 GB link
ENCFF939HLN ENCFF939HLN.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 5.2 GB link
ENCFF598HDD ENCFF598HDD.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 9.6 GB link
ENCFF911CCJ ENCFF911CCJ.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 6.8 GB link
ENCFF251IPL ENCFF251IPL.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 122.4 MB link
ENCFF975CLT ENCFF975CLT.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 103.3 MB link
ENCFF593ZON ENCFF593ZON.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 107.3 MB link
ENCFF634WKB ENCFF634WKB.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 90.8 MB link
ENCFF562QOF ENCFF562QOF.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 123.7 MB link
ENCFF803XQD ENCFF803XQD.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 104.4 MB link
ENCFF474AML ENCFF474AML.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 109.5 MB link
ENCFF836FGO ENCFF836FGO.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 92.7 MB link
ENCFF268YAX ENCFF268YAX.fastq fastq (reads) reads 2.5 GB link
ENCFF489YIZ ENCFF489YIZ.fastq fastq (reads) reads 1.9 GB link
ENCFF532LRG ENCFF532LRG.fastq fastq (reads) reads 2.7 GB link
ENCFF573BMX ENCFF573BMX.fastq fastq (reads) reads 1.8 GB link
ENCFF088BBE ENCFF088BBE.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF300NPE ENCFF300NPE.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF088DUC ENCFF088DUC.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.4 MB link
ENCFF406ABS ENCFF406ABS.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.5 MB link
ENCFF852OIY ENCFF852OIY.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.9 MB link
ENCFF929IAQ ENCFF929IAQ.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.8 MB link