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ENCSR654SOD

ENCSR ENCODE
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RNA-seq on K562 cells treated with a CRISPR gRNA against AGFG1. (AGFG1-BGKcLV26)

Organism: Homo sapiens
Experiment Types:
CRISPR RNA-seq
Status: released
Contact: Brenton Graveley, UConn

Summary

CRISPR RNA-seq of AGFG1 in Homo sapiens K562 genetically modified (deletion) using CRISPR targeting H. sapiens AGFG1

Overall Design

RNA-seq on K562 cells treated with a CRISPR gRNA against AGFG1. (AGFG1-BGKcLV26)

Analysis page is available for ENCSR654SOD.

ENCODE Metadata

Total file size: 21.8 GB
Assay: CRISPR RNA-seq
Target: AGFG1
Biosample: Homo sapiens K562 genetically modified (deletion) using CRISPR targeting H. sapiens AGFG1
Files: 22
Unique ENC file IDs: 22
File Formats
bigWig (8) tsv (6) bam (4) fastq (4)
Output types (9)
reads (4) transcript quantifications (4) alignments (2) gene quantifications (2) minus strand signal of all reads (2) minus strand signal of unique reads (2) plus strand signal of all reads (2) plus strand signal of unique reads (2) transcriptome alignments (2)

Data Files (22)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
ENCFF175AQP ENCFF175AQP.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 2.7 GB link
ENCFF994JKY ENCFF994JKY.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 2.7 GB link
ENCFF081ISN ENCFF081ISN.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 5.0 GB link
ENCFF222QUK ENCFF222QUK.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 5.2 GB link
ENCFF256QQV ENCFF256QQV.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 75.1 MB link
ENCFF929XHV ENCFF929XHV.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 82.4 MB link
ENCFF366RUY ENCFF366RUY.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 66.4 MB link
ENCFF998WMC ENCFF998WMC.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 72.5 MB link
ENCFF726HHC ENCFF726HHC.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 83.2 MB link
ENCFF825JTU ENCFF825JTU.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 75.8 MB link
ENCFF039ESS ENCFF039ESS.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 73.9 MB link
ENCFF843HSH ENCFF843HSH.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 67.7 MB link
ENCFF196VIH ENCFF196VIH.fastq fastq (reads) reads 1.3 GB link
ENCFF249BQE ENCFF249BQE.fastq fastq (reads) reads 1.4 GB link
ENCFF329NNW ENCFF329NNW.fastq fastq (reads) reads 1.4 GB link
ENCFF689RZI ENCFF689RZI.fastq fastq (reads) reads 1.3 GB link
ENCFF339QXK ENCFF339QXK.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF387JFH ENCFF387JFH.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF291ISJ ENCFF291ISJ.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.4 MB link
ENCFF496GKV ENCFF496GKV.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.8 MB link
ENCFF548COF ENCFF548COF.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.3 MB link
ENCFF817USJ ENCFF817USJ.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.8 MB link