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ENCSR585MSI

ENCSR ENCODE
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RNA-seq on HepG2 cells treated with a CRISPR gRNA against API5. (API5-BGHcLV27)

Organism: Homo sapiens
Experiment Types:
CRISPR RNA-seq
Status: released
Contact: Brenton Graveley, UConn

Summary

CRISPR RNA-seq of API5 in Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens API5

Overall Design

RNA-seq on HepG2 cells treated with a CRISPR gRNA against API5. (API5-BGHcLV27)

Analysis page is available for ENCSR585MSI.

ENCODE Metadata

Total file size: 24.2 GB
Assay: CRISPR RNA-seq
Target: API5
Biosample: Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens API5
Files: 22
Unique ENC file IDs: 22
File Formats
bigWig (8) tsv (6) bam (4) fastq (4)
Output types (9)
reads (4) transcript quantifications (4) alignments (2) gene quantifications (2) minus strand signal of all reads (2) minus strand signal of unique reads (2) plus strand signal of all reads (2) plus strand signal of unique reads (2) transcriptome alignments (2)

Data Files (22)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
ENCFF888VEB ENCFF888VEB.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 3.0 GB link
ENCFF916DHQ ENCFF916DHQ.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 2.8 GB link
ENCFF466KXV ENCFF466KXV.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 5.6 GB link
ENCFF691UJC ENCFF691UJC.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 5.9 GB link
ENCFF462AWO ENCFF462AWO.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 79.7 MB link
ENCFF773VVE ENCFF773VVE.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 83.1 MB link
ENCFF224LCV ENCFF224LCV.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 74.7 MB link
ENCFF965YSM ENCFF965YSM.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 71.9 MB link
ENCFF300ARG ENCFF300ARG.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 81.1 MB link
ENCFF575ALC ENCFF575ALC.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 84.6 MB link
ENCFF482FLF ENCFF482FLF.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 73.8 MB link
ENCFF691GMH ENCFF691GMH.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 76.7 MB link
ENCFF073CJW ENCFF073CJW.fastq fastq (reads) reads 1.6 GB link
ENCFF540HYJ ENCFF540HYJ.fastq fastq (reads) reads 1.4 GB link
ENCFF668PUW ENCFF668PUW.fastq fastq (reads) reads 1.5 GB link
ENCFF882VZY ENCFF882VZY.fastq fastq (reads) reads 1.5 GB link
ENCFF745FUH ENCFF745FUH.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF937OXO ENCFF937OXO.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF228TVT ENCFF228TVT.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.8 MB link
ENCFF532TOL ENCFF532TOL.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.8 MB link
ENCFF651TKL ENCFF651TKL.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.4 MB link
ENCFF947FGM ENCFF947FGM.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.3 MB link