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ENCSR293VAR

ENCSR ENCODE
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RNA-seq on HepG2 cells treated with a CRISPR gRNA against SPAG9. (SPAG9-BGHcLV31)

Organism: Homo sapiens
Experiment Types:
CRISPR RNA-seq
Status: released
Contact: Brenton Graveley, UConn

Summary

CRISPR RNA-seq of SPAG9 in Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens SPAG9

Overall Design

RNA-seq on HepG2 cells treated with a CRISPR gRNA against SPAG9. (SPAG9-BGHcLV31)

Analysis page is available for ENCSR293VAR.

ENCODE Metadata

Total file size: 31.0 GB
Assay: CRISPR RNA-seq
Target: SPAG9
Biosample: Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens SPAG9
Files: 22
Unique ENC file IDs: 22
File Formats
bigWig (8) tsv (6) bam (4) fastq (4)
Output types (9)
reads (4) transcript quantifications (4) alignments (2) gene quantifications (2) minus strand signal of all reads (2) minus strand signal of unique reads (2) plus strand signal of all reads (2) plus strand signal of unique reads (2) transcriptome alignments (2)

Data Files (22)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
ENCFF053NKA ENCFF053NKA.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 3.0 GB link
ENCFF766SSO ENCFF766SSO.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 4.4 GB link
ENCFF939QBJ ENCFF939QBJ.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 9.1 GB link
ENCFF955RKD ENCFF955RKD.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 5.9 GB link
ENCFF071QKD ENCFF071QKD.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 86.2 MB link
ENCFF771OGX ENCFF771OGX.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 105.7 MB link
ENCFF040EVY ENCFF040EVY.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 77.3 MB link
ENCFF755OBX ENCFF755OBX.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 95.4 MB link
ENCFF697PXX ENCFF697PXX.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 107.5 MB link
ENCFF959VGB ENCFF959VGB.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 87.6 MB link
ENCFF570EDC ENCFF570EDC.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 97.8 MB link
ENCFF807ATJ ENCFF807ATJ.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 79.2 MB link
ENCFF128XLV ENCFF128XLV.fastq fastq (reads) reads 2.4 GB link
ENCFF342WBP ENCFF342WBP.fastq fastq (reads) reads 1.6 GB link
ENCFF373LUX ENCFF373LUX.fastq fastq (reads) reads 1.5 GB link
ENCFF929XDN ENCFF929XDN.fastq fastq (reads) reads 2.3 GB link
ENCFF410ERA ENCFF410ERA.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF629GGP ENCFF629GGP.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF496LPZ ENCFF496LPZ.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.9 MB link
ENCFF540BGZ ENCFF540BGZ.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.5 MB link
ENCFF791EPZ ENCFF791EPZ.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.4 MB link
ENCFF837IAK ENCFF837IAK.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.8 MB link