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ENCSR077MFQ

ENCSR ENCODE
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RNA-seq on HepG2 cells treated with a CRISPR gRNA against CARS. (CARS-BGHcLV27)

Organism: Homo sapiens
Experiment Types:
CRISPR RNA-seq
Status: released
Contact: Brenton Graveley, UConn

Summary

CRISPR RNA-seq of CARS in Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens CARS

Overall Design

RNA-seq on HepG2 cells treated with a CRISPR gRNA against CARS. (CARS-BGHcLV27)

Analysis page is available for ENCSR077MFQ.

ENCODE Metadata

Total file size: 24.2 GB
Assay: CRISPR RNA-seq
Target: CARS
Biosample: Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens CARS
Files: 22
Unique ENC file IDs: 22
File Formats
bigWig (8) tsv (6) bam (4) fastq (4)
Output types (9)
reads (4) transcript quantifications (4) alignments (2) gene quantifications (2) minus strand signal of all reads (2) minus strand signal of unique reads (2) plus strand signal of all reads (2) plus strand signal of unique reads (2) transcriptome alignments (2)

Data Files (22)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
ENCFF088VIC ENCFF088VIC.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 3.0 GB link
ENCFF296AWM ENCFF296AWM.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 2.7 GB link
ENCFF389MLJ ENCFF389MLJ.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 5.5 GB link
ENCFF546SCQ ENCFF546SCQ.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 6.2 GB link
ENCFF298UID ENCFF298UID.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 80.9 MB link
ENCFF884EKC ENCFF884EKC.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 84.8 MB link
ENCFF479TDR ENCFF479TDR.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 72.0 MB link
ENCFF773YRY ENCFF773YRY.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 76.2 MB link
ENCFF362XPP ENCFF362XPP.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 86.1 MB link
ENCFF441REA ENCFF441REA.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 82.1 MB link
ENCFF421MRW ENCFF421MRW.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 78.1 MB link
ENCFF660ZCW ENCFF660ZCW.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 73.8 MB link
ENCFF096POD ENCFF096POD.fastq fastq (reads) reads 1.6 GB link
ENCFF104RHT ENCFF104RHT.fastq fastq (reads) reads 1.4 GB link
ENCFF156LGW ENCFF156LGW.fastq fastq (reads) reads 1.5 GB link
ENCFF268QCX ENCFF268QCX.fastq fastq (reads) reads 1.6 GB link
ENCFF633DQB ENCFF633DQB.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF678PJX ENCFF678PJX.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF132BZA ENCFF132BZA.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.4 MB link
ENCFF673QHR ENCFF673QHR.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.3 MB link
ENCFF907VDK ENCFF907VDK.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.8 MB link
ENCFF938DJB ENCFF938DJB.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.8 MB link