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ENCSR335KFN

ENCSR ENCODE
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RNA-seq on K562 cells treated with a CRISPR gRNA against METTL3. (METTL3-BGKcLV23)

Organism: Homo sapiens
Experiment Types:
CRISPR RNA-seq
Status: released
Contact: Brenton Graveley, UConn

Summary

CRISPR RNA-seq of METTL3 in Homo sapiens K562 genetically modified (deletion) using CRISPR targeting H. sapiens METTL3

Overall Design

RNA-seq on K562 cells treated with a CRISPR gRNA against METTL3. (METTL3-BGKcLV23)

Analysis page is available for ENCSR335KFN.

ENCODE Metadata

Total file size: 64.2 GB
Assay: CRISPR RNA-seq
Target: METTL3
Biosample: Homo sapiens K562 genetically modified (deletion) using CRISPR targeting H. sapiens METTL3
Files: 22
Unique ENC file IDs: 22
File Formats
bigWig (8) tsv (6) bam (4) fastq (4)
Output types (9)
reads (4) transcript quantifications (4) alignments (2) gene quantifications (2) minus strand signal of all reads (2) minus strand signal of unique reads (2) plus strand signal of all reads (2) plus strand signal of unique reads (2) transcriptome alignments (2)

Data Files (22)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
ENCFF476VSH ENCFF476VSH.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 5.2 GB link
ENCFF611QPO ENCFF611QPO.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 10.6 GB link
ENCFF519RWR ENCFF519RWR.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 9.7 GB link
ENCFF875AOF ENCFF875AOF.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 20.5 GB link
ENCFF542GEE ENCFF542GEE.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 125.0 MB link
ENCFF875EXU ENCFF875EXU.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 167.8 MB link
ENCFF024QQQ ENCFF024QQQ.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 148.7 MB link
ENCFF167ETT ENCFF167ETT.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 111.0 MB link
ENCFF060KGZ ENCFF060KGZ.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 169.9 MB link
ENCFF740GQV ENCFF740GQV.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 126.6 MB link
ENCFF860VYS ENCFF860VYS.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 152.0 MB link
ENCFF885KUS ENCFF885KUS.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 113.6 MB link
ENCFF140KLQ ENCFF140KLQ.fastq fastq (reads) reads 5.9 GB link
ENCFF170IXA ENCFF170IXA.fastq fastq (reads) reads 5.6 GB link
ENCFF332DGA ENCFF332DGA.fastq fastq (reads) reads 2.7 GB link
ENCFF831XZB ENCFF831XZB.fastq fastq (reads) reads 2.8 GB link
ENCFF334ZCM ENCFF334ZCM.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.5 MB link
ENCFF443LWE ENCFF443LWE.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF373NEC ENCFF373NEC.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 30.0 MB link
ENCFF558AYF ENCFF558AYF.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.5 MB link
ENCFF721PRM ENCFF721PRM.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.9 MB link
ENCFF994RVM ENCFF994RVM.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.6 MB link