← All datasets

ENCSR235JSA

ENCSR ENCODE
Export SRA CSV View on ENCODE

RNA-seq on HepG2 cells treated with a CRISPR gRNA against RPUSD2. (RPUSD2-BGHcLV31)

Organism: Homo sapiens
Experiment Types:
CRISPR RNA-seq
Status: released
Contact: Brenton Graveley, UConn

Summary

CRISPR RNA-seq of RPUSD2 in Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens RPUSD2

Overall Design

RNA-seq on HepG2 cells treated with a CRISPR gRNA against RPUSD2. (RPUSD2-BGHcLV31)

Analysis page is available for ENCSR235JSA.

ENCODE Metadata

Total file size: 30.8 GB
Assay: CRISPR RNA-seq
Target: RPUSD2
Biosample: Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens RPUSD2
Files: 22
Unique ENC file IDs: 22
File Formats
bigWig (8) tsv (6) bam (4) fastq (4)
Output types (9)
reads (4) transcript quantifications (4) alignments (2) gene quantifications (2) minus strand signal of all reads (2) minus strand signal of unique reads (2) plus strand signal of all reads (2) plus strand signal of unique reads (2) transcriptome alignments (2)

Data Files (22)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
ENCFF104NVV ENCFF104NVV.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 3.9 GB link
ENCFF678QSP ENCFF678QSP.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 3.5 GB link
ENCFF537IWC ENCFF537IWC.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 7.0 GB link
ENCFF972BHN ENCFF972BHN.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 7.8 GB link
ENCFF145THA ENCFF145THA.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 94.6 MB link
ENCFF975ZUC ENCFF975ZUC.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 92.7 MB link
ENCFF177SJM ENCFF177SJM.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 85.5 MB link
ENCFF514JKB ENCFF514JKB.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 82.3 MB link
ENCFF026DNP ENCFF026DNP.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 94.4 MB link
ENCFF197WOY ENCFF197WOY.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 96.3 MB link
ENCFF171TVP ENCFF171TVP.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 87.8 MB link
ENCFF456EGO ENCFF456EGO.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 84.5 MB link
ENCFF132YUC ENCFF132YUC.fastq fastq (reads) reads 1.9 GB link
ENCFF374NSH ENCFF374NSH.fastq fastq (reads) reads 2.0 GB link
ENCFF455BJO ENCFF455BJO.fastq fastq (reads) reads 1.8 GB link
ENCFF588DHI ENCFF588DHI.fastq fastq (reads) reads 2.1 GB link
ENCFF022WHD ENCFF022WHD.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF714CEB ENCFF714CEB.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF136DJS ENCFF136DJS.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.8 MB link
ENCFF182LWT ENCFF182LWT.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.3 MB link
ENCFF211MBP ENCFF211MBP.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.4 MB link
ENCFF439FHB ENCFF439FHB.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.8 MB link