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ENCSR218ITU

ENCSR ENCODE
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RNA-seq on K562 cells treated with a CRISPR gRNA against SEC31A. (SEC31A-BGKcLV33)

Organism: Homo sapiens
Experiment Types:
CRISPR RNA-seq
Status: released
Contact: Brenton Graveley, UConn

Summary

CRISPR RNA-seq of SEC31A in Homo sapiens K562 genetically modified (deletion) using CRISPR targeting H. sapiens SEC31A

Overall Design

RNA-seq on K562 cells treated with a CRISPR gRNA against SEC31A. (SEC31A-BGKcLV33)

Analysis page is available for ENCSR218ITU.

ENCODE Metadata

Total file size: 34.9 GB
Assay: CRISPR RNA-seq
Target: SEC31A
Biosample: Homo sapiens K562 genetically modified (deletion) using CRISPR targeting H. sapiens SEC31A
Files: 22
Unique ENC file IDs: 22
File Formats
bigWig (8) tsv (6) bam (4) fastq (4)
Output types (9)
reads (4) transcript quantifications (4) alignments (2) gene quantifications (2) minus strand signal of all reads (2) minus strand signal of unique reads (2) plus strand signal of all reads (2) plus strand signal of unique reads (2) transcriptome alignments (2)

Data Files (22)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
ENCFF511MXS ENCFF511MXS.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 5.7 GB link
ENCFF585ZYO ENCFF585ZYO.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 3.6 GB link
ENCFF323CDS ENCFF323CDS.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 9.1 GB link
ENCFF745ESF ENCFF745ESF.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 5.8 GB link
ENCFF569NGO ENCFF569NGO.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 157.5 MB link
ENCFF623PYH ENCFF623PYH.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 122.1 MB link
ENCFF295ZNV ENCFF295ZNV.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 141.2 MB link
ENCFF660WCH ENCFF660WCH.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 109.2 MB link
ENCFF225JYG ENCFF225JYG.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 160.5 MB link
ENCFF563LVH ENCFF563LVH.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 124.4 MB link
ENCFF031AWU ENCFF031AWU.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 112.3 MB link
ENCFF927OCB ENCFF927OCB.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 145.3 MB link
ENCFF186PLZ ENCFF186PLZ.fastq fastq (reads) reads 1.8 GB link
ENCFF393HYM ENCFF393HYM.fastq fastq (reads) reads 1.8 GB link
ENCFF619CGF ENCFF619CGF.fastq fastq (reads) reads 2.9 GB link
ENCFF654UKO ENCFF654UKO.fastq fastq (reads) reads 2.8 GB link
ENCFF527ZOE ENCFF527ZOE.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF658XUI ENCFF658XUI.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF296NFC ENCFF296NFC.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.5 MB link
ENCFF466IRC ENCFF466IRC.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.4 MB link
ENCFF551DFH ENCFF551DFH.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.9 MB link
ENCFF939WHX ENCFF939WHX.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.8 MB link