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ENCSR300RMY

ENCSR ENCODE
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RNA-seq on HepG2 cells treated with a CRISPR gRNA against GSK3A. (GSK3A-BGHcLV14)

Organism: Homo sapiens
Experiment Types:
CRISPR RNA-seq
Status: released
Contact: Brenton Graveley, UConn

Summary

CRISPR RNA-seq of GSK3A in Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens GSK3A

Overall Design

RNA-seq on HepG2 cells treated with a CRISPR gRNA against GSK3A. (GSK3A-BGHcLV14)

Analysis page is available for ENCSR300RMY.

ENCODE Metadata

Total file size: 18.0 GB
Assay: CRISPR RNA-seq
Target: GSK3A
Biosample: Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens GSK3A
Files: 22
Unique ENC file IDs: 22
File Formats
bigWig (8) tsv (6) bam (4) fastq (4)
Output types (9)
reads (4) transcript quantifications (4) alignments (2) gene quantifications (2) minus strand signal of all reads (2) minus strand signal of unique reads (2) plus strand signal of all reads (2) plus strand signal of unique reads (2) transcriptome alignments (2)

Data Files (22)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
ENCFF128ZST ENCFF128ZST.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 2.5 GB link
ENCFF368BXB ENCFF368BXB.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 1.8 GB link
ENCFF381FSS ENCFF381FSS.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 5.0 GB link
ENCFF818VIJ ENCFF818VIJ.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 3.6 GB link
ENCFF161WSL ENCFF161WSL.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 79.7 MB link
ENCFF360NFR ENCFF360NFR.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 65.2 MB link
ENCFF504MPH ENCFF504MPH.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 71.4 MB link
ENCFF687FIT ENCFF687FIT.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 58.2 MB link
ENCFF426MJR ENCFF426MJR.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 66.3 MB link
ENCFF508NHZ ENCFF508NHZ.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 81.1 MB link
ENCFF424OUV ENCFF424OUV.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 73.0 MB link
ENCFF496PVG ENCFF496PVG.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 59.5 MB link
ENCFF220NYZ ENCFF220NYZ.fastq fastq (reads) reads 1.3 GB link
ENCFF525TWL ENCFF525TWL.fastq fastq (reads) reads 899.7 MB link
ENCFF901BLS ENCFF901BLS.fastq fastq (reads) reads 1.2 GB link
ENCFF996DZC ENCFF996DZC.fastq fastq (reads) reads 940.5 MB link
ENCFF097YXW ENCFF097YXW.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF424EMD ENCFF424EMD.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF148NYN ENCFF148NYN.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.8 MB link
ENCFF467NAX ENCFF467NAX.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.3 MB link
ENCFF656UWE ENCFF656UWE.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.3 MB link
ENCFF718GRR ENCFF718GRR.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.8 MB link