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4 results for “Protein tagging”
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APEX Proximity Labeling of Stress Granule Proteins.
Methods in molecular biology (Clifton, N.J.) · 2022 · 2428: 381-399
Ascorbate peroxidase (APEX)-catalyzed proximity labeling has been recently established as a robust approach to uncover localized protein environments and transient protein-protein interactions occurring across mammalian cells. This molecular tool enables improved identification of individual proteins localized to and involved in …
PMID 35171492 DOI ↗
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Peptide Brush Polymers for Efficient Delivery of a Gene Editing Protein to Stem Cells.
Angewandte Chemie (International ed. in English) · 2019 · 58(44): 15646-15649
The scarcity of effective means to deliver functional proteins to living cells is a central problem in biotechnology and medicine. Herein, we report the efficient delivery of an active DNA-modifying enzyme to human stem cells through high-density cell penetrating peptide …
PMID 31404473 DOI ↗
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Active Protein Neddylation or Ubiquitylation Is Dispensable for Stress Granule Dynamics.
Cell reports · 2019 · 27(5): 1356-1363.e3
Stress granule (SG) formation is frequently accompanied by ubiquitin proteasome system (UPS) impairment and ubiquitylated protein accumulation. SGs, ubiquitin, and UPS components co-localize, but the relationship between the ubiquitin pathway and SGs has not been systematically characterized. We utilize pharmacological …
PMID 31042464 DOI ↗
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CRISPR/Cas9-mediated integration enables TAG-eCLIP of endogenously tagged RNA binding proteins.
Methods (San Diego, Calif.) · 2017 · 118-119: 50-59
Identification of in vivo direct RNA targets for RNA binding proteins (RBPs) provides critical insight into their regulatory activities and mechanisms. Recently, we described a methodology for enhanced crosslinking and immunoprecipitation followed by high-throughput sequencing (eCLIP) using antibodies against endogenous …
PMID 28003131 DOI ↗
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