GSE95238 Data Processing

geo_data_processing 3 steps

Publication

Early transcriptional and epigenetic regulation of CD8<sup>+</sup> T cell differentiation revealed by single-cell RNA sequencing.

Nature immunology (2017) — PMID 28218746

Dataset

GSE95238

Epigenetic landscapes reveal transcription factors that regulate CD8+ T cell differentiation

Warning: Pipeline descriptions and code snippets may be inferred or AI-generated. Use them only as a starting point to guide analysis, and validate before use.

Processing Steps

Generate Jupyter Notebook
  1. 1

    Reads were aligned to mm10 by BWA

    BWA
  2. 2

    Reads with low quality (MAPQ < 30) were filtered out.

  3. 3

    If multiple reads were mapped to the exact same location, only one read was kept.

Raw Source Text 
Reads were aligned to mm10 by BWA
Reads with low quality (MAPQ < 30) were filtered out. If multiple reads were mapped to the exact same location, only one read was kept.
Genome_build: mm10
Supplementary_files_format_and_content: bigwig
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