Early transcriptional and epigenetic regulation of CD8<sup>+</sup> T cell differentiation revealed by single-cell RNA sequencing.
Boyko Kakaradov,
Janilyn Arsenio,
Christella E Widjaja,
Zhaoren He,
Stefan Aigner,
Patrick J Metz,
Bingfei Yu,
Ellen J Wehrens,
Justine Lopez,
Stephanie H Kim,
Elina I Zuniga,
Ananda W Goldrath,
John T Chang,
Gene W Yeo
Nature immunology
· 2017
· Vol. 18
(4)
· pp. 422-432
Abstract
During microbial infection, responding CD8<sup>+</sup> T lymphocytes differentiate into heterogeneous subsets that together provide immediate and durable protection. To elucidate the dynamic transcriptional changes that underlie this process, we applied a single-cell RNA-sequencing approach and analyzed individual CD8<sup>+</sup> T lymphocytes sequentially throughout the course of a viral infection in vivo. Our analyses revealed a striking transcriptional divergence among cells that had undergone their first division and identified previously unknown molecular determinants that controlled the fate specification of CD8<sup>+</sup> T lymphocytes. Our findings suggest a model for the differentiation of terminal effector cells initiated by an early burst of transcriptional activity and subsequently refined by epigenetic silencing of transcripts associated with memory lymphocytes, which highlights the power and necessity of single-cell approaches.
Publication Types
["Journal Article", "Research Support, Non-U.S. Gov't", "Research Support, N.I.H., Extramural"]
Keywords
MeSH Terms
["Animals", "CD8-Positive T-Lymphocytes", "Cell Differentiation", "Cluster Analysis", "Computational Biology", "Epigenesis, Genetic", "Gene Expression Profiling", "Gene Silencing", "Genetic Heterogeneity", "Histones", "Immunologic Memory", "Lymphocyte Activation", "Mice", "Sequence Analysis, RNA", "Single-Cell Analysis", "T-Lymphocyte Subsets", "Transcription, Genetic", "Transcriptome"]
Funding
R01 HG004659
NHGRI NIH HHS
(United States)
R01 AI113923
NIAID NIH HHS
(United States)
R01 AI072117
NIAID NIH HHS
(United States)
U19 MH107367
NIMH NIH HHS
(United States)
Howard Hughes Medical Institute
(United States)
T32 DK007202
NIDDK NIH HHS
(United States)
R01 NS075449
NINDS NIH HHS
(United States)
R01 AI095277
NIAID NIH HHS
(United States)
R01 AI096852
NIAID NIH HHS
(United States)
R01 DK093507
NIDDK NIH HHS
(United States)
R01 AI081923
NIAID NIH HHS
(United States)
DP2 OD008469
NIH HHS
(United States)
Linked Datasets (4)
Genome-wide maps of H3K27me3 in CD8 T cell subsets
Mus musculus54 data files
| File | Type | Size |
|---|---|---|
| Memory_ATAC-seq_rep1.fastq | OTHER | 381.4 MB |
| Memory_ATAC-seq_rep2.fastq | OTHER | 1.0 GB |
| Memory_H3K27ac_rep1.fastq | ChIP-Seq | 969.7 MB |
| Memory_H3K27ac_rep2.fastq | ChIP-Seq | 326.1 MB |
| Memory_H3K27me3_rep1.fastq.gz | ChIP-Seq | 875.2 MB |
| Memory_H3K27me3_rep2.fastq.gz | ChIP-Seq | 280.5 MB |
| Memory_H3K4me1_rep1.fastq | ChIP-Seq | 1.1 GB |
| Memory_H3K4me1_rep2.fastq | ChIP-Seq | 343.6 MB |
| Memory_H3K4me3_rep1.fastq | ChIP-Seq | 848.8 MB |
| Memory_H3K4me3_rep2.fastq | ChIP-Seq | 266.6 MB |
| Memory_Input_rep1.fastq | ChIP-Seq | 644.1 MB |
| Memory_Input_rep2.fastq | ChIP-Seq | 361.4 MB |
| MP_ATAC-seq_rep1.fastq | OTHER | 684.1 MB |
| MP_ATAC-seq_rep2.fastq | OTHER | 622.3 MB |
| MP_H3K27ac_rep1.fastq | ChIP-Seq | 708.3 MB |
| MP_H3K27ac_rep2.fastq | ChIP-Seq | 691.2 MB |
| MP_H3K27me3_rep1.fastq.gz | ChIP-Seq | 420.0 MB |
| MP_H3K27me3_rep2.fastq.gz | ChIP-Seq | 394.2 MB |
| MP_H3K4me1_rep1.fastq | ChIP-Seq | 891.6 MB |
| MP_H3K4me1_rep2.fastq | ChIP-Seq | 861.7 MB |
| MP_H3K4me3_rep1.fastq | ChIP-Seq | 615.8 MB |
| MP_H3K4me3_rep2.fastq | ChIP-Seq | 540.4 MB |
| MP_Input_rep1.fastq | ChIP-Seq | 355.3 MB |
| MP_Input_rep2.fastq | ChIP-Seq | 753.4 MB |
| SRR4437318 | OTHER | 622.3 MB |
| SRR4437320 | OTHER | 1.0 GB |
| SRR4437329 | ChIP-Seq | 609.9 MB |
| SRR4437339 | ChIP-Seq | 1.0 GB |
| SRR4437341 | ChIP-Seq | 420.0 MB |
| SRR4437342 | ChIP-Seq | 394.2 MB |
| TE_ATAC-seq_rep1.fastq | OTHER | 733.3 MB |
| TE_ATAC-seq_rep2.fastq | OTHER | 649.2 MB |
| TE_H3K27ac_rep1.fastq | ChIP-Seq | 669.8 MB |
| TE_H3K27ac_rep2.fastq | ChIP-Seq | 323.9 MB |
| TE_H3K27me3_rep1.fastq.gz | ChIP-Seq | 1.0 GB |
| TE_H3K27me3_rep2.fastq.gz | ChIP-Seq | 239.6 MB |
| TE_H3K4me1_rep1.fastq | ChIP-Seq | 921.6 MB |
| TE_H3K4me1_rep2.fastq | ChIP-Seq | 1.0 GB |
| TE_H3K4me3_rep1.fastq | ChIP-Seq | 430.5 MB |
| TE_H3K4me3_rep2.fastq | ChIP-Seq | 686.9 MB |
| TE_Input_rep1.fastq | ChIP-Seq | 1.1 GB |
| TE_Input_rep2.fastq | ChIP-Seq | 346.4 MB |
| TN_ATAC-seq_rep1.fastq | OTHER | 882.3 MB |
| TN_ATAC-seq_rep2.fastq | OTHER | 678.1 MB |
| TN_H3K27ac_rep1.fastq | ChIP-Seq | 730.0 MB |
| TN_H3K27ac_rep2.fastq | ChIP-Seq | 432.8 MB |
| TN_H3K27me3_rep1.fastq.gz | ChIP-Seq | 348.8 MB |
| TN_H3K27me3_rep2.fastq.gz | ChIP-Seq | 319.9 MB |
| TN_H3K4me1_rep1.fastq | ChIP-Seq | 609.9 MB |
| TN_H3K4me1_rep2.fastq | ChIP-Seq | 727.1 MB |
| TN_H3K4me3_rep1.fastq | ChIP-Seq | 991.9 MB |
| TN_H3K4me3_rep2.fastq | ChIP-Seq | 719.4 MB |
| TN_Input_rep1.fastq | ChIP-Seq | 355.2 MB |
| TN_Input_rep2.fastq | ChIP-Seq | 319.8 MB |
Early transcriptional and epigenetic regulation of CD8+ T cell differentiation revealed by single-cell RNA-seq
Mus musculus304 data files
| File | Type | Size |
|---|---|---|
| 1A10_CGAGGCTG_L001_R1.fastq.gz | RNA-Seq | 791.9 MB |
| 1A11_AAGAGGCA_L001_R1.fastq.gz | RNA-Seq | 613.7 MB |
| 1A12_GTAGAGGA_L001_R1.fastq.gz | RNA-Seq | 4.6 GB |
| 1A1_TAAGGCGA_L001_R1.fastq.gz | RNA-Seq | 666.2 MB |
| 1A2_CGTACTAG_L001_R1.fastq.gz | RNA-Seq | 1.2 GB |
| 1A3_AGGCAGAA_L001_R1.fastq.gz | RNA-Seq | 1.7 GB |
| 1A4_TCCTGAGC_L001_R1.fastq.gz | RNA-Seq | 1.0 GB |
| 1A5_GGACTCCT_L001_R1.fastq.gz | RNA-Seq | 1.9 GB |
| 1A6_TAGGCATG_L001_R1.fastq.gz | RNA-Seq | 1.1 GB |
| 1A7_CTCTCTAC_L001_R1.fastq.gz | RNA-Seq | 1.3 GB |
| 1A8_CAGAGAGG_L001_R1.fastq.gz | RNA-Seq | 1.2 GB |
| 1A9_GCTACGCT_L001_R1.fastq.gz | RNA-Seq | 2.3 GB |
| 1B10_CGAGGCTG_L002_R1.fastq.gz | RNA-Seq | 517.5 MB |
| 1B11_AAGAGGCA_L002_R1.fastq.gz | RNA-Seq | 1.6 GB |
| 1B12_GTAGAGGA_L002_R1.fastq.gz | RNA-Seq | 1.2 GB |
| 1B1_TAAGGCGA_L002_R1.fastq.gz | RNA-Seq | 497.4 MB |
| 1B2_CGTACTAG_L002_R1.fastq.gz | RNA-Seq | 1.8 GB |
| 1B3_AGGCAGAA_L002_R1.fastq.gz | RNA-Seq | 1.9 GB |
| 1B4_TCCTGAGC_L002_R1.fastq.gz | RNA-Seq | 1.6 GB |
| 1B5_GGACTCCT_L002_R1.fastq.gz | RNA-Seq | 2.1 GB |
| 1B6_TAGGCATG_L002_R1.fastq.gz | RNA-Seq | 2.2 GB |
| 1B7_CTCTCTAC_L002_R1.fastq.gz | RNA-Seq | 1.9 GB |
| 1B8_CAGAGAGG_L002_R1.fastq.gz | RNA-Seq | 610.2 MB |
| 1B9_GCTACGCT_L002_R1.fastq.gz | RNA-Seq | 2.1 GB |
| 1C10_CGAGGCTG_L003_R1.fastq.gz | RNA-Seq | 726.3 MB |
| 1C11_AAGAGGCA_L003_R1.fastq.gz | RNA-Seq | 2.0 GB |
| 1C12_GTAGAGGA_L003_R1.fastq.gz | RNA-Seq | 862.5 MB |
| 1C1_TAAGGCGA_L003_R1.fastq.gz | RNA-Seq | 923.7 MB |
| 1C2_CGTACTAG_L003_R1.fastq.gz | RNA-Seq | 581.0 MB |
| 1C3_AGGCAGAA_L003_R1.fastq.gz | RNA-Seq | 776.7 MB |
| 1C4_TCCTGAGC_L003_R1.fastq.gz | RNA-Seq | 1.3 GB |
| 1C5_GGACTCCT_L003_R1.fastq.gz | RNA-Seq | 1.6 GB |
| 1C6_TAGGCATG_L003_R1.fastq.gz | RNA-Seq | 1.3 GB |
| 1C7_CTCTCTAC_L003_R1.fastq.gz | RNA-Seq | 1.7 GB |
| 1C8_CAGAGAGG_L003_R1.fastq.gz | RNA-Seq | 2.9 GB |
| 1C9_GCTACGCT_L003_R1.fastq.gz | RNA-Seq | 1.8 GB |
| 1D10_CGAGGCTG_L004_R1.fastq.gz | RNA-Seq | 1.1 GB |
| 1D11_AAGAGGCA_L004_R1.fastq.gz | RNA-Seq | 2.1 GB |
| 1D12_GTAGAGGA_L004_R1.fastq.gz | RNA-Seq | 1.5 GB |
| 1D1_TAAGGCGA_L004_R1.fastq.gz | RNA-Seq | 1.7 GB |
| 1D2_CGTACTAG_L004_R1.fastq.gz | RNA-Seq | 1.6 GB |
| 1D3_AGGCAGAA_L004_R1.fastq.gz | RNA-Seq | 1.7 GB |
| 1D4_TCCTGAGC_L004_R1.fastq.gz | RNA-Seq | 1.8 GB |
| 1D5_GGACTCCT_L004_R1.fastq.gz | RNA-Seq | 1.6 GB |
| 1D6_TAGGCATG_L004_R1.fastq.gz | RNA-Seq | 502.5 MB |
| 1D7_CTCTCTAC_L004_R1.fastq.gz | RNA-Seq | 1.6 GB |
| 1D8_CAGAGAGG_L004_R1.fastq.gz | RNA-Seq | 1.4 GB |
| 1D9_GCTACGCT_L004_R1.fastq.gz | RNA-Seq | 1.0 GB |
| 1E10_CGAGGCTG_L005_R1.fastq.gz | RNA-Seq | 1023.6 MB |
| 1E11_AAGAGGCA_L005_R1.fastq.gz | RNA-Seq | 2.9 GB |
| 1E12_GTAGAGGA_L005_R1.fastq.gz | RNA-Seq | 1.9 GB |
| 1E1_TAAGGCGA_L005_R1.fastq.gz | RNA-Seq | 1.2 GB |
| 1E2_CGTACTAG_L005_R1.fastq.gz | RNA-Seq | 661.6 MB |
| 1E3_AGGCAGAA_L005_R1.fastq.gz | RNA-Seq | 1.9 GB |
| 1E4_TCCTGAGC_L005_R1.fastq.gz | RNA-Seq | 1.8 GB |
| 1E5_GGACTCCT_L005_R1.fastq.gz | RNA-Seq | 1.3 GB |
| 1E6_TAGGCATG_L005_R1.fastq.gz | RNA-Seq | 2.4 GB |
| 1E7_CTCTCTAC_L005_R1.fastq.gz | RNA-Seq | 2.1 GB |
| 1E8_CAGAGAGG_L005_R1.fastq.gz | RNA-Seq | 462.1 MB |
| 1E9_GCTACGCT_L005_R1.fastq.gz | RNA-Seq | 1.6 GB |
| 1F10_CGAGGCTG_L006_R1.fastq.gz | RNA-Seq | 1.7 GB |
| 1F11_AAGAGGCA_L006_R1.fastq.gz | RNA-Seq | 2.2 GB |
| 1F12_GTAGAGGA_L006_R1.fastq.gz | RNA-Seq | 653.4 MB |
| 1F1_TAAGGCGA_L006_R1.fastq.gz | RNA-Seq | 1.1 GB |
| 1F2_CGTACTAG_L006_R1.fastq.gz | RNA-Seq | 383.9 MB |
| 1F3_AGGCAGAA_L006_R1.fastq.gz | RNA-Seq | 2.6 GB |
| 1F4_TCCTGAGC_L006_R1.fastq.gz | RNA-Seq | 814.4 MB |
| 1F5_GGACTCCT_L006_R1.fastq.gz | RNA-Seq | 1.8 GB |
| 1F6_TAGGCATG_L006_R1.fastq.gz | RNA-Seq | 1.8 GB |
| 1F7_CTCTCTAC_L006_R1.fastq.gz | RNA-Seq | 1.9 GB |
| 1F8_CAGAGAGG_L006_R1.fastq.gz | RNA-Seq | 711.6 MB |
| 1F9_GCTACGCT_L006_R1.fastq.gz | RNA-Seq | 1.8 GB |
| 1G10_CGAGGCTG_L007_R1.fastq.gz | RNA-Seq | 757.2 MB |
| 1G11_AAGAGGCA_L007_R1.fastq.gz | RNA-Seq | 1.9 GB |
| 1G12_GTAGAGGA_L007_R1.fastq.gz | RNA-Seq | 610.3 MB |
| 1G1_TAAGGCGA_L007_R1.fastq.gz | RNA-Seq | 1.5 GB |
| 1G2_CGTACTAG_L007_R1.fastq.gz | RNA-Seq | 857.3 MB |
| 1G3_AGGCAGAA_L007_R1.fastq.gz | RNA-Seq | 2.0 GB |
| 1G4_TCCTGAGC_L007_R1.fastq.gz | RNA-Seq | 1.7 GB |
| 1G5_GGACTCCT_L007_R1.fastq.gz | RNA-Seq | 1.7 GB |
| 1G6_TAGGCATG_L007_R1.fastq.gz | RNA-Seq | 1.3 GB |
| 1G7_CTCTCTAC_L007_R1.fastq.gz | RNA-Seq | 2.6 GB |
| 1G8_CAGAGAGG_L007_R1.fastq.gz | RNA-Seq | 616.4 MB |
| 1G9_GCTACGCT_L007_R1.fastq.gz | RNA-Seq | 2.6 GB |
| 1H10_CGAGGCTG_L008_R1.fastq.gz | RNA-Seq | 925.1 MB |
| 1H11_AAGAGGCA_L008_R1.fastq.gz | RNA-Seq | 1.3 GB |
| 1H12_GTAGAGGA_L008_R1.fastq.gz | RNA-Seq | 1.1 GB |
| 1H1_TAAGGCGA_L008_R1.fastq.gz | RNA-Seq | 1.4 GB |
| 1H2_CGTACTAG_L008_R1.fastq.gz | RNA-Seq | 435.4 MB |
| 1H3_AGGCAGAA_L008_R1.fastq.gz | RNA-Seq | 158.9 MB |
| 1H4_TCCTGAGC_L008_R1.fastq.gz | RNA-Seq | 733.2 MB |
| 1H5_GGACTCCT_L008_R1.fastq.gz | RNA-Seq | 1.5 GB |
| 1H6_TAGGCATG_L008_R1.fastq.gz | RNA-Seq | 524.7 MB |
| 1H7_CTCTCTAC_L008_R1.fastq.gz | RNA-Seq | 1.2 GB |
| 1H8_CAGAGAGG_L008_R1.fastq.gz | RNA-Seq | 1.3 GB |
| 1H9_GCTACGCT_L008_R1.fastq.gz | RNA-Seq | 1.6 GB |
| 2A10_CGAGGCTG_L001_R1.fastq.gz | RNA-Seq | 887.3 MB |
| 2A11_AAGAGGCA_L001_R1.fastq.gz | RNA-Seq | 949.5 MB |
| 2A12_GTAGAGGA_L001_R1.fastq.gz | RNA-Seq | 957.1 MB |
| 2A1_TAAGGCGA_L001_R1.fastq.gz | RNA-Seq | 948.5 MB |
| 2A2_CGTACTAG_L001_R1.fastq.gz | RNA-Seq | 700.3 MB |
| 2A3_AGGCAGAA_L001_R1.fastq.gz | RNA-Seq | 2.0 GB |
| 2A4_TCCTGAGC_L001_R1.fastq.gz | RNA-Seq | 753.6 MB |
| 2A5_GGACTCCT_L001_R1.fastq.gz | RNA-Seq | 1.4 GB |
| 2A6_TAGGCATG_L001_R1.fastq.gz | RNA-Seq | 940.2 MB |
| 2A7_CTCTCTAC_L001_R1.fastq.gz | RNA-Seq | 1.0 GB |
| 2A8_CAGAGAGG_L001_R1.fastq.gz | RNA-Seq | 1.5 GB |
| 2A9_GCTACGCT_L001_R1.fastq.gz | RNA-Seq | 1.0 GB |
| 2B10_CGAGGCTG_L002_R1.fastq.gz | RNA-Seq | 877.3 MB |
| 2B11_AAGAGGCA_L002_R1.fastq.gz | RNA-Seq | 764.0 MB |
| 2B12_GTAGAGGA_L002_R1.fastq.gz | RNA-Seq | 1.3 GB |
| 2B1_TAAGGCGA_L002_R1.fastq.gz | RNA-Seq | 1.3 GB |
| 2B2_CGTACTAG_L002_R1.fastq.gz | RNA-Seq | 310.3 MB |
| 2B3_AGGCAGAA_L002_R1.fastq.gz | RNA-Seq | 1.4 GB |
| 2B4_TCCTGAGC_L002_R1.fastq.gz | RNA-Seq | 1.4 GB |
| 2B5_GGACTCCT_L002_R1.fastq.gz | RNA-Seq | 2.2 GB |
| 2B6_TAGGCATG_L002_R1.fastq.gz | RNA-Seq | 759.1 MB |
| 2B7_CTCTCTAC_L002_R1.fastq.gz | RNA-Seq | 1020.7 MB |
| 2B8_CAGAGAGG_L002_R1.fastq.gz | RNA-Seq | 717.9 MB |
| 2B9_GCTACGCT_L002_R1.fastq.gz | RNA-Seq | 1.5 GB |
| 2C10_CGAGGCTG_L003_R1.fastq.gz | RNA-Seq | 1.2 GB |
| 2C11_AAGAGGCA_L003_R1.fastq.gz | RNA-Seq | 848.8 MB |
| 2C12_GTAGAGGA_L003_R1.fastq.gz | RNA-Seq | 1.3 GB |
| 2C1_TAAGGCGA_L003_R1.fastq.gz | RNA-Seq | 776.2 MB |
| 2C2_CGTACTAG_L003_R1.fastq.gz | RNA-Seq | 488.7 MB |
| 2C3_AGGCAGAA_L003_R1.fastq.gz | RNA-Seq | 1.9 GB |
| 2C4_TCCTGAGC_L003_R1.fastq.gz | RNA-Seq | 655.7 MB |
| 2C5_GGACTCCT_L003_R1.fastq.gz | RNA-Seq | 1.5 GB |
| 2C6_TAGGCATG_L003_R1.fastq.gz | RNA-Seq | 1.7 GB |
| 2C7_CTCTCTAC_L003_R1.fastq.gz | RNA-Seq | 1.0 GB |
| 2C8_CAGAGAGG_L003_R1.fastq.gz | RNA-Seq | 1.3 GB |
| 2C9_GCTACGCT_L003_R1.fastq.gz | RNA-Seq | 1.1 GB |
| 2D10_CGAGGCTG_L004_R1.fastq.gz | RNA-Seq | 752.4 MB |
| 2D11_AAGAGGCA_L004_R1.fastq.gz | RNA-Seq | 674.8 MB |
| 2D12_GTAGAGGA_L004_R1.fastq.gz | RNA-Seq | 1.3 GB |
| 2D1_TAAGGCGA_L004_R1.fastq.gz | RNA-Seq | 622.8 MB |
| 2D2_CGTACTAG_L004_R1.fastq.gz | RNA-Seq | 634.8 MB |
| 2D3_AGGCAGAA_L004_R1.fastq.gz | RNA-Seq | 1.2 GB |
| 2D4_TCCTGAGC_L004_R1.fastq.gz | RNA-Seq | 1.2 GB |
| 2D5_GGACTCCT_L004_R1.fastq.gz | RNA-Seq | 365.7 MB |
| 2D6_TAGGCATG_L004_R1.fastq.gz | RNA-Seq | 1.1 GB |
| 2D7_CTCTCTAC_L004_R1.fastq.gz | RNA-Seq | 867.4 MB |
| 2D8_CAGAGAGG_L004_R1.fastq.gz | RNA-Seq | 1007.2 MB |
| 2D9_GCTACGCT_L004_R1.fastq.gz | RNA-Seq | 642.9 MB |
| 2E10_CGAGGCTG_L005_R1.fastq.gz | RNA-Seq | 932.9 MB |
| 2E11_AAGAGGCA_L005_R1.fastq.gz | RNA-Seq | 757.9 MB |
| 2E12_GTAGAGGA_L005_R1.fastq.gz | RNA-Seq | 972.9 MB |
| 2E1_TAAGGCGA_L005_R1.fastq.gz | RNA-Seq | 468.6 MB |
| 2E2_CGTACTAG_L005_R1.fastq.gz | RNA-Seq | 819.2 MB |
| 2E3_AGGCAGAA_L005_R1.fastq.gz | RNA-Seq | 1.7 GB |
| 2E4_TCCTGAGC_L005_R1.fastq.gz | RNA-Seq | 940.4 MB |
| 2E5_GGACTCCT_L005_R1.fastq.gz | RNA-Seq | 2.0 GB |
| 2E6_TAGGCATG_L005_R1.fastq.gz | RNA-Seq | 1.2 GB |
| 2E7_CTCTCTAC_L005_R1.fastq.gz | RNA-Seq | 1.1 GB |
| 2E8_CAGAGAGG_L005_R1.fastq.gz | RNA-Seq | 1.1 GB |
| 2E9_GCTACGCT_L005_R1.fastq.gz | RNA-Seq | 1.5 GB |
| 2F10_CGAGGCTG_L006_R1.fastq.gz | RNA-Seq | 605.5 MB |
| 2F11_AAGAGGCA_L006_R1.fastq.gz | RNA-Seq | 1.3 GB |
| 2F12_GTAGAGGA_L006_R1.fastq.gz | RNA-Seq | 1.2 GB |
| 2F1_TAAGGCGA_L006_R1.fastq.gz | RNA-Seq | 1.2 GB |
| 2F2_CGTACTAG_L006_R1.fastq.gz | RNA-Seq | 1.0 GB |
| 2F3_AGGCAGAA_L006_R1.fastq.gz | RNA-Seq | 1.0 GB |
| 2F4_TCCTGAGC_L006_R1.fastq.gz | RNA-Seq | 1.5 GB |
| 2F5_GGACTCCT_L006_R1.fastq.gz | RNA-Seq | 329.6 MB |
| 2F6_TAGGCATG_L006_R1.fastq.gz | RNA-Seq | 1.2 GB |
| 2F7_CTCTCTAC_L006_R1.fastq.gz | RNA-Seq | 1.2 GB |
| 2F8_CAGAGAGG_L006_R1.fastq.gz | RNA-Seq | 1.2 GB |
| 2F9_GCTACGCT_L006_R1.fastq.gz | RNA-Seq | 1.3 GB |
| 2G10_CGAGGCTG_L007_R1.fastq.gz | RNA-Seq | 1.5 GB |
| 2G11_AAGAGGCA_L007_R1.fastq.gz | RNA-Seq | 736.7 MB |
| 2G12_GTAGAGGA_L007_R1.fastq.gz | RNA-Seq | 1.7 GB |
| 2G1_TAAGGCGA_L007_R1.fastq.gz | RNA-Seq | 571.3 MB |
| 2G2_CGTACTAG_L007_R1.fastq.gz | RNA-Seq | 392.9 MB |
| 2G3_AGGCAGAA_L007_R1.fastq.gz | RNA-Seq | 1.6 GB |
| 2G4_TCCTGAGC_L007_R1.fastq.gz | RNA-Seq | 630.3 MB |
| 2G5_GGACTCCT_L007_R1.fastq.gz | RNA-Seq | 1.2 GB |
| 2G6_TAGGCATG_L007_R1.fastq.gz | RNA-Seq | 1.6 GB |
| 2G7_CTCTCTAC_L007_R1.fastq.gz | RNA-Seq | 1.3 GB |
| 2G8_CAGAGAGG_L007_R1.fastq.gz | RNA-Seq | 1.2 GB |
| 2G9_GCTACGCT_L007_R1.fastq.gz | RNA-Seq | 1.3 GB |
| 2H10_CGAGGCTG_L008_R1.fastq.gz | RNA-Seq | 746.1 MB |
| 2H11_AAGAGGCA_L008_R1.fastq.gz | RNA-Seq | 916.8 MB |
| 2H12_GTAGAGGA_L008_R1.fastq.gz | RNA-Seq | 917.0 MB |
| 2H1_TAAGGCGA_L008_R1.fastq.gz | RNA-Seq | 977.6 MB |
| 2H2_CGTACTAG_L008_R1.fastq.gz | RNA-Seq | 417.8 MB |
| 2H3_AGGCAGAA_L008_R1.fastq.gz | RNA-Seq | 1.3 GB |
| 2H4_TCCTGAGC_L008_R1.fastq.gz | RNA-Seq | 1.3 GB |
| 2H5_GGACTCCT_L008_R1.fastq.gz | RNA-Seq | 1.6 GB |
| 2H6_TAGGCATG_L008_R1.fastq.gz | RNA-Seq | 424.0 MB |
| 2H7_CTCTCTAC_L008_R1.fastq.gz | RNA-Seq | 782.6 MB |
| 2H8_CAGAGAGG_L008_R1.fastq.gz | RNA-Seq | 1.0 GB |
| 2H9_GCTACGCT_L008_R1.fastq.gz | RNA-Seq | 2.3 GB |
| 3A10_CGAGGCTG-GCGTAAGA_L001_R1.fastq.gz | RNA-Seq | 575.3 MB |
| 3A11_AAGAGGCA-GCGTAAGA_L001_R1.fastq.gz | RNA-Seq | 658.9 MB |
| 3A12_GTAGAGGA-GCGTAAGA_L001_R1.fastq.gz | RNA-Seq | 599.1 MB |
| 3A1_TAAGGCGA-GCGTAAGA_L001_R1.fastq.gz | RNA-Seq | 503.0 MB |
| 3A2_CGTACTAG-GCGTAAGA_L001_R1.fastq.gz | RNA-Seq | 792.6 MB |
| 3A3_AGGCAGAA-GCGTAAGA_L001_R1.fastq.gz | RNA-Seq | 766.5 MB |
| 3A4_TCCTGAGC-GCGTAAGA_L001_R1.fastq.gz | RNA-Seq | 205.6 MB |
| 3A5_GGACTCCT-GCGTAAGA_L001_R1.fastq.gz | RNA-Seq | 526.4 MB |
| 3A6_TAGGCATG-GCGTAAGA_L001_R1.fastq.gz | RNA-Seq | 769.1 MB |
| 3A7_CTCTCTAC-GCGTAAGA_L001_R1.fastq.gz | RNA-Seq | 663.6 MB |
| 3A8_CAGAGAGG-GCGTAAGA_L001_R1.fastq.gz | RNA-Seq | 584.2 MB |
| 3A9_GCTACGCT-GCGTAAGA_L001_R1.fastq.gz | RNA-Seq | 335.5 MB |
| 3B10_CGAGGCTG-CTCTCTAT_L002_R1.fastq.gz | RNA-Seq | 729.5 MB |
| 3B11_AAGAGGCA-CTCTCTAT_L002_R1.fastq.gz | RNA-Seq | 847.3 MB |
| 3B12_GTAGAGGA-CTCTCTAT_L002_R1.fastq.gz | RNA-Seq | 879.1 MB |
| 3B1_TAAGGCGA-CTCTCTAT_L002_R1.fastq.gz | RNA-Seq | 793.5 MB |
| 3B2_CGTACTAG-CTCTCTAT_L002_R1.fastq.gz | RNA-Seq | 732.2 MB |
| 3B3_AGGCAGAA-CTCTCTAT_L002_R1.fastq.gz | RNA-Seq | 1.3 GB |
| 3B4_TCCTGAGC-CTCTCTAT_L002_R1.fastq.gz | RNA-Seq | 1.3 GB |
| 3B5_GGACTCCT-CTCTCTAT_L002_R1.fastq.gz | RNA-Seq | 976.6 MB |
| 3B6_TAGGCATG-CTCTCTAT_L002_R1.fastq.gz | RNA-Seq | 820.1 MB |
| 3B7_CTCTCTAC-CTCTCTAT_L002_R1.fastq.gz | RNA-Seq | 920.9 MB |
| 3B8_CAGAGAGG-CTCTCTAT_L002_R1.fastq.gz | RNA-Seq | 759.1 MB |
| 3B9_GCTACGCT-CTCTCTAT_L002_R1.fastq.gz | RNA-Seq | 932.8 MB |
| 3C10_CGAGGCTG-TATCCTCT_L003_R1.fastq.gz | RNA-Seq | 621.1 MB |
| 3C11_AAGAGGCA-TATCCTCT_L003_R1.fastq.gz | RNA-Seq | 842.9 MB |
| 3C12_GTAGAGGA-TATCCTCT_L003_R1.fastq.gz | RNA-Seq | 543.8 MB |
| 3C1_TAAGGCGA-TATCCTCT_L003_R1.fastq.gz | RNA-Seq | 557.6 MB |
| 3C2_CGTACTAG-TATCCTCT_L003_R1.fastq.gz | RNA-Seq | 692.1 MB |
| 3C3_AGGCAGAA-TATCCTCT_L003_R1.fastq.gz | RNA-Seq | 1.0 GB |
| 3C4_TCCTGAGC-TATCCTCT_L003_R1.fastq.gz | RNA-Seq | 650.1 MB |
| 3C5_GGACTCCT-TATCCTCT_L003_R1.fastq.gz | RNA-Seq | 722.5 MB |
| 3C6_TAGGCATG-TATCCTCT_L003_R1.fastq.gz | RNA-Seq | 769.3 MB |
| 3C7_CTCTCTAC-TATCCTCT_L003_R1.fastq.gz | RNA-Seq | 915.0 MB |
| 3C8_CAGAGAGG-TATCCTCT_L003_R1.fastq.gz | RNA-Seq | 1.6 GB |
| 3C9_GCTACGCT-TATCCTCT_L003_R1.fastq.gz | RNA-Seq | 688.0 MB |
| 3D10_CGAGGCTG-AGAGTAGA_L004_R1.fastq.gz | RNA-Seq | 1.1 GB |
| 3D11_AAGAGGCA-AGAGTAGA_L004_R1.fastq.gz | RNA-Seq | 919.0 MB |
| 3D12_GTAGAGGA-AGAGTAGA_L004_R1.fastq.gz | RNA-Seq | 751.2 MB |
| 3D1_TAAGGCGA-AGAGTAGA_L004_R1.fastq.gz | RNA-Seq | 1.3 GB |
| 3D2_CGTACTAG-AGAGTAGA_L004_R1.fastq.gz | RNA-Seq | 1.5 GB |
| 3D3_AGGCAGAA-AGAGTAGA_L004_R1.fastq.gz | RNA-Seq | 1.3 GB |
| 3D4_TCCTGAGC-AGAGTAGA_L004_R1.fastq.gz | RNA-Seq | 1.1 GB |
| 3D5_GGACTCCT-AGAGTAGA_L004_R1.fastq.gz | RNA-Seq | 649.8 MB |
| 3D6_TAGGCATG-AGAGTAGA_L004_R1.fastq.gz | RNA-Seq | 1.5 GB |
| 3D7_CTCTCTAC-AGAGTAGA_L004_R1.fastq.gz | RNA-Seq | 798.2 MB |
| 3D8_CAGAGAGG-AGAGTAGA_L004_R1.fastq.gz | RNA-Seq | 871.3 MB |
| 3D9_GCTACGCT-AGAGTAGA_L004_R1.fastq.gz | RNA-Seq | 347.5 MB |
| 3E10_CGAGGCTG-GTAAGGAG_L005_R1.fastq.gz | RNA-Seq | 692.4 MB |
| 3E11_AAGAGGCA-GTAAGGAG_L005_R1.fastq.gz | RNA-Seq | 1.0 GB |
| 3E12_GTAGAGGA-GTAAGGAG_L005_R1.fastq.gz | RNA-Seq | 1.0 GB |
| 3E1_TAAGGCGA-GTAAGGAG_L005_R1.fastq.gz | RNA-Seq | 941.7 MB |
| 3E2_CGTACTAG-GTAAGGAG_L005_R1.fastq.gz | RNA-Seq | 765.6 MB |
| 3E3_AGGCAGAA-GTAAGGAG_L005_R1.fastq.gz | RNA-Seq | 1.2 GB |
| 3E4_TCCTGAGC-GTAAGGAG_L005_R1.fastq.gz | RNA-Seq | 815.9 MB |
| 3E5_GGACTCCT-GTAAGGAG_L005_R1.fastq.gz | RNA-Seq | 449.8 MB |
| 3E6_TAGGCATG-GTAAGGAG_L005_R1.fastq.gz | RNA-Seq | 871.0 MB |
| 3E7_CTCTCTAC-GTAAGGAG_L005_R1.fastq.gz | RNA-Seq | 689.6 MB |
| 3E8_CAGAGAGG-GTAAGGAG_L005_R1.fastq.gz | RNA-Seq | 458.9 MB |
| 3E9_GCTACGCT-GTAAGGAG_L005_R1.fastq.gz | RNA-Seq | 817.8 MB |
| 3F10_CGAGGCTG-ACTGCATA_L006_R1.fastq.gz | RNA-Seq | 1.2 GB |
| 3F11_AAGAGGCA-ACTGCATA_L006_R1.fastq.gz | RNA-Seq | 769.4 MB |
| 3F12_GTAGAGGA-ACTGCATA_L006_R1.fastq.gz | RNA-Seq | 700.4 MB |
| 3F1_TAAGGCGA-ACTGCATA_L006_R1.fastq.gz | RNA-Seq | 788.5 MB |
| 3F2_CGTACTAG-ACTGCATA_L006_R1.fastq.gz | RNA-Seq | 1.0 GB |
| 3F3_AGGCAGAA-ACTGCATA_L006_R1.fastq.gz | RNA-Seq | 951.8 MB |
| 3F4_TCCTGAGC-ACTGCATA_L006_R1.fastq.gz | RNA-Seq | 779.1 MB |
| 3F5_GGACTCCT-ACTGCATA_L006_R1.fastq.gz | RNA-Seq | 1.0 GB |
| 3F6_TAGGCATG-ACTGCATA_L006_R1.fastq.gz | RNA-Seq | 936.1 MB |
| 3F7_CTCTCTAC-ACTGCATA_L006_R1.fastq.gz | RNA-Seq | 1.1 GB |
| 3F8_CAGAGAGG-ACTGCATA_L006_R1.fastq.gz | RNA-Seq | 571.0 MB |
| 3F9_GCTACGCT-ACTGCATA_L006_R1.fastq.gz | RNA-Seq | 691.3 MB |
| 3G10_CGAGGCTG-AAGGAGTA_L007_R1.fastq.gz | RNA-Seq | 969.8 MB |
| 3G11_AAGAGGCA-AAGGAGTA_L007_R1.fastq.gz | RNA-Seq | 729.1 MB |
| 3G12_GTAGAGGA-AAGGAGTA_L007_R1.fastq.gz | RNA-Seq | 714.1 MB |
| 3G1_TAAGGCGA-AAGGAGTA_L007_R1.fastq.gz | RNA-Seq | 935.6 MB |
| 3G2_CGTACTAG-AAGGAGTA_L007_R1.fastq.gz | RNA-Seq | 977.8 MB |
| 3G3_AGGCAGAA-AAGGAGTA_L007_R1.fastq.gz | RNA-Seq | 1.4 GB |
| 3G4_TCCTGAGC-AAGGAGTA_L007_R1.fastq.gz | RNA-Seq | 394.8 MB |
| 3G5_GGACTCCT-AAGGAGTA_L007_R1.fastq.gz | RNA-Seq | 611.6 MB |
| 3G6_TAGGCATG-AAGGAGTA_L007_R1.fastq.gz | RNA-Seq | 717.0 MB |
| 3G7_CTCTCTAC-AAGGAGTA_L007_R1.fastq.gz | RNA-Seq | 555.0 MB |
| 3G8_CAGAGAGG-AAGGAGTA_L007_R1.fastq.gz | RNA-Seq | 1.8 GB |
| 3G9_GCTACGCT-AAGGAGTA_L007_R1.fastq.gz | RNA-Seq | 905.9 MB |
| 3H10_CGAGGCTG-CTAAGCCT_L008_R1.fastq.gz | RNA-Seq | 565.7 MB |
| 3H11_AAGAGGCA-CTAAGCCT_L008_R1.fastq.gz | RNA-Seq | 885.3 MB |
| 3H12_GTAGAGGA-CTAAGCCT_L008_R1.fastq.gz | RNA-Seq | 805.7 MB |
| 3H1_TAAGGCGA-CTAAGCCT_L008_R1.fastq.gz | RNA-Seq | 680.7 MB |
| 3H2_CGTACTAG-CTAAGCCT_L008_R1.fastq.gz | RNA-Seq | 831.0 MB |
| 3H3_AGGCAGAA-CTAAGCCT_L008_R1.fastq.gz | RNA-Seq | 925.3 MB |
| 3H4_TCCTGAGC-CTAAGCCT_L008_R1.fastq.gz | RNA-Seq | 934.4 MB |
| 3H5_GGACTCCT-CTAAGCCT_L008_R1.fastq.gz | RNA-Seq | 1005.1 MB |
| 3H6_TAGGCATG-CTAAGCCT_L008_R1.fastq.gz | RNA-Seq | 379.7 MB |
| 3H7_CTCTCTAC-CTAAGCCT_L008_R1.fastq.gz | RNA-Seq | 1.0 GB |
| 3H8_CAGAGAGG-CTAAGCCT_L008_R1.fastq.gz | RNA-Seq | 2.2 GB |
| 3H9_GCTACGCT-CTAAGCCT_L008_R1.fastq.gz | RNA-Seq | 544.5 MB |
| CW05_S5_L002_R1_001.fastq.gz | RNA-Seq | 965.3 MB |
| CW06_S6_L002_R1_001.fastq.gz | RNA-Seq | 916.1 MB |
| CW07_S7_L002_R1_001.fastq.gz | RNA-Seq | 953.7 MB |
| CW08_S8_L002_R1_001.fastq.gz | RNA-Seq | 860.4 MB |
| H3K27me3_KO1.fastq.gz | ChIP-Seq | 968.4 MB |
| H3K27me3_KO2.fastq.gz | ChIP-Seq | 1.2 GB |
| H3K27me3_WT1.fastq.gz | ChIP-Seq | 851.4 MB |
| H3K27me3_WT2.fastq.gz | ChIP-Seq | 1.2 GB |
| Input_KO1.fastq.gz | ChIP-Seq | 2.1 GB |
| Input_KO2.fastq.gz | ChIP-Seq | 1.6 GB |
| Input_WT1.fastq.gz | ChIP-Seq | 1.6 GB |
| Input_WT2.fastq.gz | ChIP-Seq | 1.8 GB |
| SAD4EZH3_S31_L008_R1_001.fastq.gz | ChIP-Seq | 321.6 MB |
| SAD4H3K3_S33_L008_R1_001.fastq.gz | ChIP-Seq | 552.9 MB |
| SAD4INP1_S39_L008_R1_001.fastq.gz | ChIP-Seq | 325.2 MB |
| SAD4POL3_S32_L008_R1_001.fastq.gz | ChIP-Seq | 502.9 MB |
Genome-wide maps of chromatin state and chromatin accessibility in CD8 T cell subsets
Mus musculus40 data files
| File | Type | Size |
|---|---|---|
| SRR5305074 | OTHER | 882.3 MB |
| SRR5305075 | OTHER | 678.1 MB |
| SRR5305076 | OTHER | 733.3 MB |
| SRR5305077 | OTHER | 649.2 MB |
| SRR5305078 | OTHER | 684.1 MB |
| SRR5305079.sralite | OTHER | 622.3 MB |
| SRR5305080 | OTHER | 381.4 MB |
| SRR5305081 | OTHER | 1.0 GB |
| SRR5305082 | ChIP-Seq | 991.9 MB |
| SRR5305083.sralite | ChIP-Seq | 719.4 MB |
| SRR5305084 | ChIP-Seq | 430.5 MB |
| SRR5305085 | ChIP-Seq | 686.9 MB |
| SRR5305086 | ChIP-Seq | 615.8 MB |
| SRR5305087 | ChIP-Seq | 540.4 MB |
| SRR5305088 | ChIP-Seq | 848.8 MB |
| SRR5305089 | ChIP-Seq | 266.6 MB |
| SRR5305090 | ChIP-Seq | 609.9 MB |
| SRR5305091 | ChIP-Seq | 727.1 MB |
| SRR5305092 | ChIP-Seq | 921.6 MB |
| SRR5305093 | ChIP-Seq | 1.0 GB |
| SRR5305094 | ChIP-Seq | 891.6 MB |
| SRR5305095 | ChIP-Seq | 861.7 MB |
| SRR5305096 | ChIP-Seq | 1.1 GB |
| SRR5305097 | ChIP-Seq | 343.6 MB |
| SRR5305098 | ChIP-Seq | 730.0 MB |
| SRR5305099.sralite | ChIP-Seq | 432.8 MB |
| SRR5305100.sralite | ChIP-Seq | 669.8 MB |
| SRR5305101 | ChIP-Seq | 323.9 MB |
| SRR5305102 | ChIP-Seq | 708.3 MB |
| SRR5305103 | ChIP-Seq | 691.2 MB |
| SRR5305104 | ChIP-Seq | 969.7 MB |
| SRR5305105.sralite | ChIP-Seq | 326.1 MB |
| SRR5305106 | ChIP-Seq | 355.2 MB |
| SRR5305107 | ChIP-Seq | 319.8 MB |
| SRR5305108.sralite | ChIP-Seq | 1.1 GB |
| SRR5305109 | ChIP-Seq | 346.4 MB |
| SRR5305110.sralite | ChIP-Seq | 355.3 MB |
| SRR5305111 | ChIP-Seq | 753.4 MB |
| SRR5305112.sralite | ChIP-Seq | 644.1 MB |
| SRR5305113.sralite | ChIP-Seq | 361.4 MB |
Analysis Pipelines (4)
GSE89036 Data Processing
3 steps
geo_data_processing
GSE89036
GSE89405 RNA-seq Data Processing
39 steps
GSE95237 Data Processing
3 steps
geo_data_processing
GSE95237
GSE95238 Data Processing
3 steps
geo_data_processing
GSE95238