GSE315443 scRNA-seq Data Processing

scRNA-seq geo_data_processing 2 steps

Publication

MBNL2 dysfunction in outer radial glial cells is associated with disrupted corticogenesis in congenital myotonic dystrophy.

Neurobiology of disease (2026) — PMID 41644016

Dataset

GSE315443

MBNL2 dysfunction in outer radial glial cells is associated with disrupted corticogenesis in congenital myotonic dystrophy [scRNA-Seq]

Warning: Pipeline descriptions and code snippets may be inferred or AI-generated. Use them only as a starting point to guide analysis, and validate before use.

Processing Steps

Generate Jupyter Notebook
  1. 1

    Through 10× Cell Ranger's pipeline, the raw base call (BCL) files were demultiplexed into FASTQ files with the 10x Genomics Cell Ranger pipeline.

    Cell Ranger
  2. 2

    Raw sequencing data were processed using Cell Ranger v7.2.0 (10x Genomics) to perform alignment, filtering, barcode assignment and UMI counting, with intronic reads included in the counting matrix.

    Cell Ranger v7.2.0
Raw Source Text 
Through 10× Cell Ranger's pipeline, the raw base call (BCL) files were demultiplexed into FASTQ files with the 10x Genomics Cell Ranger pipeline. Raw sequencing data were processed using Cell Ranger v7.2.0 (10x Genomics) to perform alignment, filtering, barcode assignment and UMI counting, with intronic reads included in the counting matrix.
Assembly: GRCh38
Supplementary files format and content: Tab-separated values and sparse matrix files corresponding to barcode, feature, and gene expression data
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