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GSE157050

GSE GEO
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Ribosome profiling of metformin treatment in primary murine hepatocytes in WT, Raptor Ser-Ala mutant, Tsc2-null, Raptor mutant;Tsc2-null, and Ampk-null cells

Organism: Mus musculus
Platform: GPL17021
Samples: 66
Experiment Types:
Expression profiling by high throughput sequencing Other
Submitted: Aug 28 2020
Last Updated: Aug 10 2023
Status: Public on Aug 10 2023
Contact: April,Elizabeth,Williams (Salk Institute for Biological Studies)

Relations

SubSeries of: GSE157051 BioProject: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA659995 SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRP279200

Summary

Despite being the frontline therapy for Type 2 diabetes, the mechanisms of action of the biguanide drug metformin are still being discovered. In particular, the detailed molecular interplays between the AMPK and the mTORC1 pathway in the hepatic benefits of metformin are still ill-defined. Metformin-dependent activation of AMPK classically inhibits mTORC1 via TSC/RHEB. But several lines of evidence suggest additional mechanisms at play in metformin inhibition of mTORC1. Here we investigated the role of direct AMPK-mediated serine phosphorylation of RAPTOR in a new RaptorAA mouse model, in which AMPK phospho-serine sites Ser722 and Ser792 of RAPTOR were mutated to alanine. Metformin treatment of primary hepatocytes and intact murine liver requires AMPK regulation of both RAPTOR and TSC2 to fully inhibit mTORC1, and this regulation is critical for the translational response to metformin.

Overall Design

2h treatment of 2mM Metformin or 0.1uM INK-128 in serum-starved primary murine hepatocytes in the absence or presence of 15 minute pre-treatment with 1nM Insulin

Analysis (6 steps)

View Data Processing
Processing steps for GSE157050
  1. First, adapter trimming was performed with cutadapt (v1.14).
  2. Next, reads were mapped with STAR (v2.4.0i) against a database of repetitive elements, with mapping reads discarded from further analysis.
  3. Remaining reads were then mapped to the mouse genome (mm10) with STAR (v2.4.0i)
  4. Stranded reads mapping to GENCODE (vM20) genes were quantified with featureCounts (subread-1.6.4).
  5. Mitochondrial genes were discarded, and only genes with at least 0.5 reads per million across all datasets were kept for further analysis.
  6. Differential expression in polysome versus input was quantified with edgeR (v3.26.8) with dispersion = 0.06 estimated from the subset of experiments with multiple replicates.

Supplementary Files (22)

GSE157050_AATSC_ins_PvsI.edgeR.txt.gz Download
GSE157050_AATSC_insmet_PvsI.edgeR.txt.gz Download
GSE157050_AATSC_met_PvsI.edgeR.txt.gz Download
GSE157050_AATSC_ut_PvsI.edgeR.txt.gz Download
GSE157050_AA_ins_PvsI.edgeR.txt.gz Download
GSE157050_AA_insmet_PvsI.edgeR.txt.gz Download
GSE157050_AA_met_PvsI.edgeR.txt.gz Download
GSE157050_AA_ut_PvsI.edgeR.txt.gz Download
GSE157050_AMPK_ins_PvsI.edgeR.txt.gz Download
GSE157050_AMPK_insmet_PvsI.edgeR.txt.gz Download
GSE157050_AMPK_met_PvsI.edgeR.txt.gz Download
GSE157050_AMPK_ut_PvsI.edgeR.txt.gz Download
GSE157050_TSC_ins_PvsI.edgeR.txt.gz Download
GSE157050_TSC_insmet_PvsI.edgeR.txt.gz Download
GSE157050_TSC_met_PvsI.edgeR.txt.gz Download
GSE157050_TSC_ut_PvsI.edgeR.txt.gz Download
GSE157050_WT_ink_PvsI.edgeR.txt.gz Download
GSE157050_WT_ins_PvsI.edgeR.txt.gz Download
GSE157050_WT_insink_PvsI.edgeR.txt.gz Download
GSE157050_WT_insmet_PvsI.edgeR.txt.gz Download
GSE157050_WT_met_PvsI.edgeR.txt.gz Download
GSE157050_WT_ut_PvsI.edgeR.txt.gz Download
GEO Samples (66)
GSM4751640 GSM4751641 GSM4751642 GSM4751643 GSM4751644 GSM4751645 GSM4751646 GSM4751647 GSM4751648 GSM4751649 GSM4751650 GSM4751651 GSM4751652 GSM4751653 GSM4751654 GSM4751655 GSM4751656 GSM4751657 GSM4751658 GSM4751659 GSM4751660 GSM4751661 GSM4751662 GSM4751663 GSM4751664 GSM4751665 GSM4751666 GSM4751667 GSM4751668 GSM4751669 GSM4751670 GSM4751671 GSM4751672 GSM4751673 GSM4751674 GSM4751675 GSM4751676 GSM4751677 GSM4751678 GSM4751679 GSM4751680 GSM4751681 GSM4751682 GSM4751683 GSM4751684 GSM4751685 GSM4751686 GSM4751687 GSM4751688 GSM4751689 GSM4751690 GSM4751691 GSM4751692 GSM4751693 GSM4751694 GSM4751695 GSM4751696 GSM4751697 GSM4751698 GSM4751699 GSM4751700 GSM4751701 GSM4751702 GSM4751703 GSM4751704 GSM4751705

Dataset Citations (1)

AMPK regulation of Raptor and TSC2 mediate metformin effects on transcriptional control of anabolism and inflammation.
PMID 32912901 · 2020 · Genes & development
Jeanine L Van Nostrand, Kristina Hellberg, En-Ching Luo, Eric L Van Nostrand, Alina Dayn, Jingting Yu, Maxim N Shokhirev, Yelena Dayn, Gene W Yeo, Reuben J Shaw

SRA Experiments (66) and Runs (88)

Total: 27430 MB
SRX9029579 SRP279200 RNA-Seq SINGLE
GSM4751640: WT UT input; Mus musculus; RNA-Seq
Sample: SRS7280861
BioProject: PRJNA659995
BioSample: SAMN15931696
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: wild type
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: untreated - serum starved
insulin treatment: Untreated
rna fraction: polyA RNA
Original files (1)
Primary Hepatocytes
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR12539898 21154336 1078871136 346.05 WTUTinput_JVN1_S17_R1_001.fastq.gz, SRR12539898, SRR12539898.lite SRA
SRX9029580 SRP279200 RNA-Seq SINGLE
GSM4751641: WT Met input; Mus musculus; RNA-Seq
Sample: SRS7280863
BioProject: PRJNA659995
BioSample: SAMN15931695
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: wild type
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: 2mM Metformin for 2h serum-starved
insulin treatment: Untreated
rna fraction: polyA RNA
Original files (1)
Primary Hepatocytes
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR12539899 20515877 1046309727 336.56 WTMetinput_JVN2_S18_R1_001.fastq.gz, SRR12539899, SRR12539899.lite SRA
SRX9029581 SRP279200 RNA-Seq SINGLE
GSM4751642: WT Ins input rep1; Mus musculus; RNA-Seq
Sample: SRS7280864
BioProject: PRJNA659995
BioSample: SAMN15931694
Platform: ILLUMINA
Instrument: NextSeq 500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: wild type
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: untreated - serum starved
insulin treatment: 15 minute pre-treatment with 1nM Insulin
rna fraction: polyA RNA
Original files (1)
Primary Hepatocytes
Runs (2)
Run Spots Bases Size (MB) Files Link
SRR12539900 8171900 408595000 127.76 wt_ins_input_JVN_62_S12_R1_001.fastq.gz, SRR12539900, SRR12539900.lite SRA
SRR12539901 8817267 449680617 194.62 wt_ins_input_JVN_62_S12_R1_001.fastq-2.gz, SRR12539901, SRR12539901.l… SRA
SRX9029582 SRP279200 RNA-Seq SINGLE
GSM4751667: Ampk UT input; Mus musculus; RNA-Seq
Sample: SRS7280862
BioProject: PRJNA659995
BioSample: SAMN15931728
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Ampka1 fl/fl; Ampka2 fl/fl; AlbCreER
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: untreated - serum starved
insulin treatment: Untreated
rna fraction: polyA RNA
Original files (1)
Primary Hepatocytes
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR12539934 18573361 947241411 379.18 AMPKUTInput_JVN10_S26_R1_001.fastq.gz, SRR12539934, SRR12539934.lite SRA
SRX9029583 SRP279200 RNA-Seq SINGLE
GSM4751668: Ampk Met input; Mus musculus; RNA-Seq
Sample: SRS7280865
BioProject: PRJNA659995
BioSample: SAMN15931727
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Ampka1 fl/fl; Ampka2 fl/fl; AlbCreER
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: 2mM Metformin for 2h serum-starved
insulin treatment: Untreated
rna fraction: polyA RNA
Original files (1)
Primary Hepatocytes
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR12539935 19193628 978875028 390.53 AMPKMetInput_JVN11_S27_R1_001.fastq.gz, SRR12539935, SRR12539935.lite SRA
SRX9029584 SRP279200 RNA-Seq SINGLE
GSM4751669: Ampk Ins input rep1; Mus musculus; RNA-Seq
Sample: SRS7280866
BioProject: PRJNA659995
BioSample: SAMN15931726
Platform: ILLUMINA
Instrument: NextSeq 500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Ampka1 fl/fl; Ampka2 fl/fl; AlbCreER
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: untreated - serum starved
insulin treatment: 15 minute pre-treatment with 1nM Insulin
rna fraction: polyA RNA
Original files (1)
Primary Hepatocytes
Runs (2)
Run Spots Bases Size (MB) Files Link
SRR12539936 8129729 406486450 126.08 ampk_ins_input_JVN_70_S20_R1_001.fastq.gz, SRR12539936, SRR12539936.l… SRA
SRR12539937 22194470 1131917970 356.08 ampk_ins_input_JVN_70_S20_R1_001.fastq-2.gz, SRR12539937, SRR12539937… SRA
SRX9029585 SRP279200 RNA-Seq SINGLE
GSM4751670: Ampk Ins input rep2; Mus musculus; RNA-Seq
Sample: SRS7280868
BioProject: PRJNA659995
BioSample: SAMN15931725
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Ampka1 fl/fl; Ampka2 fl/fl; AlbCreER
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: untreated - serum starved
insulin treatment: 15 minute pre-treatment with 1nM Insulin
rna fraction: polyA RNA
Original files (1)
Primary Hepatocytes
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR12539938 18029411 919499961 372.96 AMPKInsInput_JVN21_S37_R1_001.fastq.gz, SRR12539938, SRR12539938.lite SRA
SRX9029586 SRP279200 RNA-Seq SINGLE
GSM4751671: Ampk InsMet input rep1; Mus musculus; RNA-Seq
Sample: SRS7280867
BioProject: PRJNA659995
BioSample: SAMN15931724
Platform: ILLUMINA
Instrument: NextSeq 500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Ampka1 fl/fl; Ampka2 fl/fl; AlbCreER
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: 2mM Metformin for 2h serum-starved
insulin treatment: 15 minute pre-treatment with 1nM Insulin
rna fraction: polyA RNA
Original files (1)
Primary Hepatocytes
Runs (2)
Run Spots Bases Size (MB) Files Link
SRR12539939 7031055 351552750 108.99 ampk_ins_met_input_JVN_71_S21_R1_001.fastq.gz, SRR12539939, SRR125399… SRA
SRR12539940 19424800 990664800 312.1 ampk_ins_met_input_JVN_71_S21_R1_001.fastq-2.gz, SRR12539940, SRR1253… SRA
SRX9029587 SRP279200 RNA-Seq SINGLE
GSM4751672: Ampk InsMet input rep2; Mus musculus; RNA-Seq
Sample: SRS7280869
BioProject: PRJNA659995
BioSample: SAMN15931723
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Ampka1 fl/fl; Ampka2 fl/fl; AlbCreER
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: 2mM Metformin for 2h serum-starved
insulin treatment: 15 minute pre-treatment with 1nM Insulin
rna fraction: polyA RNA
Original files (1)
Primary Hepatocytes
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR12539941 15712445 801334695 330.22 AMPKInsMetInput_JVN22_S38_R1_001.fastq.gz, SRR12539941, SRR12539941.l… SRA
SRX9029588 SRP279200 RNA-Seq SINGLE
GSM4751673: WT UT poly; Mus musculus; RNA-Seq
Sample: SRS7280870
BioProject: PRJNA659995
BioSample: SAMN15931722
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: wild type
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: untreated - serum starved
insulin treatment: Untreated
rna fraction: polysome fractionated samples
Original files (1)
Primary Hepatocytes
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR12539942 24088979 1228537929 401.48 WTUT_JVN23_S39_R1_001.fastq.gz, SRR12539942, SRR12539942.lite SRA
SRX9029589 SRP279200 OTHER SINGLE
GSM4751674: WT Met poly; Mus musculus; OTHER
Sample: SRS7280871
BioProject: PRJNA659995
BioSample: SAMN15931721
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: wild type
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: 2mM Metformin for 2h serum-starved
insulin treatment: Untreated
rna fraction: polysome fractionated samples
Original files (1)
Primary Hepatocytes
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR12539943 23151223 1180712373 385.53 WTMet_JVN24_S40_R1_001.fastq.gz, SRR12539943, SRR12539943.lite SRA
SRX9029590 SRP279200 OTHER SINGLE
GSM4751675: WT Ins poly rep1; Mus musculus; OTHER
Sample: SRS7280872
BioProject: PRJNA659995
BioSample: SAMN15931720
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: wild type
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: untreated - serum starved
insulin treatment: 15 minute pre-treatment with 1nM Insulin
rna fraction: polysome fractionated samples
Original files (1)
Primary Hepatocytes
Runs (2)
Run Spots Bases Size (MB) Files Link
SRR12539944 13420835 684462585 195.46 wt_ins_JVN_51_S1_R1_001.fastq.gz, SRR12539944, SRR12539944.lite SRA
SRR12539945 28454007 1422700350 449.55 wt_ins_JVN_51_S1_R1_001.fastq-2.gz, SRR12539945, SRR12539945.lite SRA
SRX9029591 SRP279200 OTHER SINGLE
GSM4751676: WT Ins poly rep2; Mus musculus; OTHER
Sample: SRS7280873
BioProject: PRJNA659995
BioSample: SAMN15931719
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: wild type
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: untreated - serum starved
insulin treatment: 15 minute pre-treatment with 1nM Insulin
rna fraction: polysome fractionated samples
Original files (1)
Primary Hepatocytes
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR12539946 23437908 1195333308 391.48 WTIns_JVN26_S42_R1_001.fastq.gz, SRR12539946, SRR12539946.lite SRA
SRX9029592 SRP279200 OTHER SINGLE
GSM4751677: WT InsMet poly rep1; Mus musculus; OTHER
Sample: SRS7280874
BioProject: PRJNA659995
BioSample: SAMN15931718
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: wild type
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: 2mM Metformin for 2h serum-starved
insulin treatment: 15 minute pre-treatment with 1nM Insulin
rna fraction: polysome fractionated samples
Original files (1)
Primary Hepatocytes
Runs (2)
Run Spots Bases Size (MB) Files Link
SRR12539947 20121382 1026190482 297.11 wt_ins_met_JVN_52_S2_R1_001.fastq.gz, SRR12539947, SRR12539947.lite SRA
SRR12539948 40082656 2004132800 636.28 wt_ins_met_JVN_52_S2_R1_001.fastq-2.gz, SRR12539948, SRR12539948.lite SRA
SRX9029593 SRP279200 OTHER SINGLE
GSM4751678: WT InsMet poly rep2; Mus musculus; OTHER
Sample: SRS7280875
BioProject: PRJNA659995
BioSample: SAMN15931717
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: wild type
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: 2mM Metformin for 2h serum-starved
insulin treatment: 15 minute pre-treatment with 1nM Insulin
rna fraction: polysome fractionated samples
Original files (1)
Primary Hepatocytes
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR12539949 27330744 1393867944 456.43 WTInsMet_JVN27_S43_R1_001.fastq.gz, SRR12539949, SRR12539949.lite SRA
SRX9029594 SRP279200 OTHER SINGLE
GSM4751679: WT Ink poly; Mus musculus; OTHER
Sample: SRS7280876
BioProject: PRJNA659995
BioSample: SAMN15931716
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: wild type
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: 0.1uM INK-128 for 2h serum-starved
insulin treatment: Untreated
rna fraction: polysome fractionated samples
Original files (1)
Primary Hepatocytes
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR12539950 25362292 1293476892 419.69 WtInk_JVN25_S41_R1_001.fastq.gz, SRR12539950, SRR12539950.lite SRA
SRX9029595 SRP279200 OTHER SINGLE
GSM4751680: WT InsInk poly rep1; Mus musculus; OTHER
Sample: SRS7280877
BioProject: PRJNA659995
BioSample: SAMN15931715
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: wild type
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: 0.1uM INK-128 for 2h serum-starved
insulin treatment: 15 minute pre-treatment with 1nM Insulin
rna fraction: polysome fractionated samples
Original files (1)
Primary Hepatocytes
Runs (2)
Run Spots Bases Size (MB) Files Link
SRR12539951 2954078 150657978 64.88 wt_ink_JVN_61_S11_R1_001.fastq.gz, SRR12539951, SRR12539951.lite SRA
SRR12539952 23945617 1197280850 378.14 wt_ink_JVN_61_S11_R1_001.fastq-2.gz, SRR12539952, SRR12539952.lite SRA
SRX9029596 SRP279200 OTHER SINGLE
GSM4751681: WT InsInk poly rep2; Mus musculus; OTHER
Sample: SRS7280878
BioProject: PRJNA659995
BioSample: SAMN15931714
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: wild type
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: 0.1uM INK-128 for 2h serum-starved
insulin treatment: 15 minute pre-treatment with 1nM Insulin
rna fraction: polysome fractionated samples
Original files (1)
Primary Hepatocytes
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR12539953 24931393 1271501043 411.48 WTInsInk_JVN28_S44_R1_001.fastq.gz, SRR12539953, SRR12539953.lite SRA
SRX9029597 SRP279200 OTHER SINGLE
GSM4751682: AA UT poly; Mus musculus; OTHER
Sample: SRS7280879
BioProject: PRJNA659995
BioSample: SAMN15931713
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Raptor - S722A; S792A
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: untreated - serum starved
insulin treatment: Untreated
rna fraction: polysome fractionated samples
Original files (1)
Primary Hepatocytes
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR12539954 27272335 1390889085 452.56 AAUT_JVN29_S45_R1_001.fastq.gz, SRR12539954, SRR12539954.lite SRA
SRX9029598 SRP279200 OTHER SINGLE
GSM4751683: AA Met poly; Mus musculus; OTHER
Sample: SRS7280880
BioProject: PRJNA659995
BioSample: SAMN15931712
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Raptor - S722A; S792A
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: 2mM Metformin for 2h serum-starved
insulin treatment: Untreated
rna fraction: polysome fractionated samples
Original files (1)
Primary Hepatocytes
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR12539955 26883842 1371075942 450.61 AAMet_JVN30_S46_R1_001.fastq.gz, SRR12539955, SRR12539955.lite SRA
SRX9029599 SRP279200 OTHER SINGLE
GSM4751684: AA Ins poly rep1; Mus musculus; OTHER
Sample: SRS7280882
BioProject: PRJNA659995
BioSample: SAMN15931711
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Raptor - S722A; S792A
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: untreated - serum starved
insulin treatment: 15 minute pre-treatment with 1nM Insulin
rna fraction: polysome fractionated samples
Original files (1)
Primary Hepatocytes
Runs (2)
Run Spots Bases Size (MB) Files Link
SRR12539956 10161308 518226708 148.49 aa_ins_JVN_53_S3_R1_001.fastq.gz, SRR12539956, SRR12539956.lite SRA
SRR12539957 22035874 1101793700 345.92 aa_ins_JVN_53_S3_R1_001.fastq-2.gz, SRR12539957, SRR12539957.lite SRA
SRX9029600 SRP279200 OTHER SINGLE
GSM4751685: AA Ins poly rep2; Mus musculus; OTHER
Sample: SRS7280881
BioProject: PRJNA659995
BioSample: SAMN15931710
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Raptor - S722A; S792A
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: untreated - serum starved
insulin treatment: 15 minute pre-treatment with 1nM Insulin
rna fraction: polysome fractionated samples
Original files (1)
Primary Hepatocytes
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR12539958 24464407 1247684757 408.85 AAIns_JVN31_S47_R1_001.fastq.gz, SRR12539958, SRR12539958.lite SRA
SRX9029601 SRP279200 OTHER SINGLE
GSM4751686: AA InsMet poly rep1; Mus musculus; OTHER
Sample: SRS7280883
BioProject: PRJNA659995
BioSample: SAMN15931709
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Raptor - S722A; S792A
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: 2mM Metformin for 2h serum-starved
insulin treatment: 15 minute pre-treatment with 1nM Insulin
rna fraction: polysome fractionated samples
Original files (1)
Primary Hepatocytes
Runs (2)
Run Spots Bases Size (MB) Files Link
SRR12539959 9869265 503332515 143.18 aa_ins_met_JVN_54_S4_R1_001.fastq.gz, SRR12539959, SRR12539959.lite SRA
SRR12539960 21115646 1055782300 330.69 aa_ins_met_JVN_54_S4_R1_001.fastq-2.gz, SRR12539960, SRR12539960.lite SRA
SRX9029602 SRP279200 OTHER SINGLE
GSM4751687: AA InsMet poly rep2; Mus musculus; OTHER
Sample: SRS7280884
BioProject: PRJNA659995
BioSample: SAMN15931704
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Raptor - S722A; S792A
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: 2mM Metformin for 2h serum-starved
insulin treatment: 15 minute pre-treatment with 1nM Insulin
rna fraction: polysome fractionated samples
Original files (1)
Primary Hepatocytes
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR12539961 25682088 1309786488 429.78 AAInsMet_JVN32_S48_R1_001.fastq.gz, SRR12539961, SRR12539961.lite SRA
SRX9029603 SRP279200 OTHER SINGLE
GSM4751688: Tsc UT poly; Mus musculus; OTHER
Sample: SRS7280885
BioProject: PRJNA659995
BioSample: SAMN15931708
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Tsc2 fl/fl; AlbCreEr
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: untreated - serum starved
insulin treatment: Untreated
rna fraction: polysome fractionated samples
Original files (1)
Primary Hepatocytes
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR12539962 25132386 1281751686 415.7 TscUT_JVN33_S49_R1_001.fastq.gz, SRR12539962, SRR12539962.lite SRA
SRX9029604 SRP279200 OTHER SINGLE
GSM4751689: Tsc Met Poly; Mus musculus; OTHER
Sample: SRS7280886
BioProject: PRJNA659995
BioSample: SAMN15931707
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Tsc2 fl/fl; AlbCreEr
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: 2mM Metformin for 2h serum-starved
insulin treatment: Untreated
rna fraction: polysome fractionated samples
Original files (1)
Primary Hepatocytes
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR12539963 23813166 1214471466 391.08 TscMet_JVN34_S50_R1_001.fastq.gz, SRR12539963, SRR12539963.lite SRA
SRX9029605 SRP279200 OTHER SINGLE
GSM4751690: Tsc Ins poly rep1; Mus musculus; OTHER
Sample: SRS7280887
BioProject: PRJNA659995
BioSample: SAMN15931706
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Tsc2 fl/fl; AlbCreEr
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: untreated - serum starved
insulin treatment: 15 minute pre-treatment with 1nM Insulin
rna fraction: polysome fractionated samples
Original files (1)
Primary Hepatocytes
Runs (2)
Run Spots Bases Size (MB) Files Link
SRR12539964 9543282 486707382 138.93 tsc_ins_JVN_55_S5_R1_001.fastq.gz, SRR12539964, SRR12539964.lite SRA
SRR12539965 20844771 1042238550 324.69 tsc_ins_JVN_55_S5_R1_001.fastq-2.gz, SRR12539965, SRR12539965.lite SRA
SRX9029606 SRP279200 RNA-Seq SINGLE
GSM4751643: WT Ins input rep2; Mus musculus; RNA-Seq
Sample: SRS7280888
BioProject: PRJNA659995
BioSample: SAMN15931693
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: wild type
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: untreated - serum starved
insulin treatment: 15 minute pre-treatment with 1nM Insulin
rna fraction: polyA RNA
Original files (1)
Primary Hepatocytes
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR12539902 23588860 1203031860 395.53 WTInsInput_JVN12_S28_R1_001.fastq.gz, SRR12539902, SRR12539902.lite SRA
SRX9029607 SRP279200 RNA-Seq SINGLE
GSM4751644: WT InsMet input rep1; Mus musculus; RNA-Seq
Sample: SRS7280889
BioProject: PRJNA659995
BioSample: SAMN15931692
Platform: ILLUMINA
Instrument: NextSeq 500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: wild type
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: 2mM Metformin for 2h serum-starved
insulin treatment: 15 minute pre-treatment with 1nM Insulin
rna fraction: polyA RNA
Original files (1)
Primary Hepatocytes
Runs (2)
Run Spots Bases Size (MB) Files Link
SRR12539903 7488010 374400500 116.6 wt_ins_met_input_JVN_63_S13_R1_001.fastq.gz, SRR12539903, SRR12539903… SRA
SRR12539904 20910033 1066411683 338.24 wt_ins_met_input_JVN_63_S13_R1_001.fastq-2.gz, SRR12539904, SRR125399… SRA
SRX9029608 SRP279200 RNA-Seq SINGLE
GSM4751645: WT InsMet input rep2; Mus musculus; RNA-Seq
Sample: SRS7280890
BioProject: PRJNA659995
BioSample: SAMN15931691
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: wild type
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: 2mM Metformin for 2h serum-starved
insulin treatment: 15 minute pre-treatment with 1nM Insulin
rna fraction: polyA RNA
Original files (1)
Primary Hepatocytes
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR12539905 20747845 1058140095 349.53 WTInsMetInput_JVN13_S29_R1_001.fastq.gz, SRR12539905, SRR12539905.lite SRA
SRX9029609 SRP279200 RNA-Seq SINGLE
GSM4751646: WT Ink input; Mus musculus; RNA-Seq
Sample: SRS7280891
BioProject: PRJNA659995
BioSample: SAMN15931690
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: wild type
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: 0.1uM INK-128 for 2h serum-starved
insulin treatment: Untreated
rna fraction: polyA RNA
Original files (1)
Primary Hepatocytes
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR12539906 20486574 1044815274 336.38 WtInkinput_JVN3_S19_R1_001.fastq.gz, SRR12539906, SRR12539906.lite SRA
SRX9029610 SRP279200 RNA-Seq SINGLE
GSM4751647: WT InsInk input rep1; Mus musculus; RNA-Seq
Sample: SRS7280893
BioProject: PRJNA659995
BioSample: SAMN15931689
Platform: ILLUMINA
Instrument: NextSeq 500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: wild type
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: 0.1uM INK-128 for 2h serum-starved
insulin treatment: 15 minute pre-treatment with 1nM Insulin
rna fraction: polyA RNA
Original files (1)
Primary Hepatocytes
Runs (2)
Run Spots Bases Size (MB) Files Link
SRR12539907 6973023 348651150 107.13 wt_ink_input_JVN_72_S22_R1_001.fastq.gz, SRR12539907, SRR12539907.lite SRA
SRR12539908 18869287 962333637 302.16 wt_ink_input_JVN_72_S22_R1_001.fastq-2.gz, SRR12539908, SRR12539908.l… SRA
SRX9029611 SRP279200 RNA-Seq SINGLE
GSM4751648: WT InsInk input rep2; Mus musculus; RNA-Seq
Sample: SRS7280892
BioProject: PRJNA659995
BioSample: SAMN15931688
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: wild type
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: 0.1uM INK-128 for 2h serum-starved
insulin treatment: 15 minute pre-treatment with 1nM Insulin
rna fraction: polyA RNA
Original files (1)
Primary Hepatocytes
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR12539909 19531304 996096504 328.54 WTInsInkInput_JVN14_S30_R1_001.fastq.gz, SRR12539909, SRR12539909.lite SRA
SRX9029612 SRP279200 RNA-Seq SINGLE
GSM4751649: AA UT input; Mus musculus; RNA-Seq
Sample: SRS7280894
BioProject: PRJNA659995
BioSample: SAMN15931687
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Raptor - S722A; S792A
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: untreated - serum starved
insulin treatment: Untreated
rna fraction: polyA RNA
Original files (1)
Primary Hepatocytes
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR12539910 20032264 1021645464 330.64 AAUTinput_JVN4_S20_R1_001.fastq.gz, SRR12539910, SRR12539910.lite SRA
SRX9029613 SRP279200 RNA-Seq SINGLE
GSM4751650: AA Met input; Mus musculus; RNA-Seq
Sample: SRS7280896
BioProject: PRJNA659995
BioSample: SAMN15931686
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Raptor - S722A; S792A
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: 2mM Metformin for 2h serum-starved
insulin treatment: Untreated
rna fraction: polyA RNA
Original files (1)
Primary Hepatocytes
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR12539911 20201668 1030285068 334.71 AAMetinput_JVN5_S21_R1_001.fastq.gz, SRR12539911, SRR12539911.lite SRA
SRX9029614 SRP279200 RNA-Seq SINGLE
GSM4751651: AA Ins input rep1; Mus musculus; RNA-Seq
Sample: SRS7280895
BioProject: PRJNA659995
BioSample: SAMN15931685
Platform: ILLUMINA
Instrument: NextSeq 500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Raptor - S722A; S792A
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: untreated - serum starved
insulin treatment: 15 minute pre-treatment with 1nM Insulin
rna fraction: polyA RNA
Original files (1)
Primary Hepatocytes
Runs (2)
Run Spots Bases Size (MB) Files Link
SRR12539912 8377941 418897050 130.01 aa_ins_input_JVN_64_S14_R1_001.fastq.gz, SRR12539912, SRR12539912.lite SRA
SRR12539913 23196989 1183046439 373.18 aa_ins_input_JVN_64_S14_R1_001.fastq-2.gz, SRR12539913, SRR12539913.l… SRA
SRX9029615 SRP279200 RNA-Seq SINGLE
GSM4751652: AA Ins input rep2; Mus musculus; RNA-Seq
Sample: SRS7280897
BioProject: PRJNA659995
BioSample: SAMN15931684
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Raptor - S722A; S792A
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: untreated - serum starved
insulin treatment: 15 minute pre-treatment with 1nM Insulin
rna fraction: polyA RNA
Original files (1)
Primary Hepatocytes
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR12539914 21631637 1103213487 361.46 AAInsInput_JVN15_S31_R1_001.fastq.gz, SRR12539914, SRR12539914.lite SRA
SRX9029616 SRP279200 RNA-Seq SINGLE
GSM4751653: AA InsMet input rep1; Mus musculus; RNA-Seq
Sample: SRS7280899
BioProject: PRJNA659995
BioSample: SAMN15931683
Platform: ILLUMINA
Instrument: NextSeq 500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Raptor - S722A; S792A
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: 2mM Metformin for 2h serum-starved
insulin treatment: 15 minute pre-treatment with 1nM Insulin
rna fraction: polyA RNA
Original files (1)
Primary Hepatocytes
Runs (2)
Run Spots Bases Size (MB) Files Link
SRR12539915 7184897 359244850 111.94 aa_ins_met_input_JVN_65_S15_R1_001.fastq.gz, SRR12539915, SRR12539915… SRA
SRR12539916 20237135 1032093885 327.29 aa_ins_met_input_JVN_65_S15_R1_001.fastq-2.gz, SRR12539916, SRR125399… SRA
SRX9029617 SRP279200 RNA-Seq SINGLE
GSM4751654: AA InsMet input rep2; Mus musculus; RNA-Seq
Sample: SRS7280898
BioProject: PRJNA659995
BioSample: SAMN15931682
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Raptor - S722A; S792A
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: 2mM Metformin for 2h serum-starved
insulin treatment: 15 minute pre-treatment with 1nM Insulin
rna fraction: polyA RNA
Original files (1)
Primary Hepatocytes
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR12539917 20567642 1048949742 349.84 AAInsMetInput_JVN16_S32_R1_001.fastq.gz, SRR12539917, SRR12539917.lite SRA
SRX9029618 SRP279200 RNA-Seq SINGLE
GSM4751655: Tsc UT input; Mus musculus; RNA-Seq
Sample: SRS7280901
BioProject: PRJNA659995
BioSample: SAMN15931737
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Tsc2 fl/fl; AlbCreEr
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: untreated - serum starved
insulin treatment: Untreated
rna fraction: polyA RNA
Original files (1)
Primary Hepatocytes
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR12539918 20898825 1065840075 344.87 TscUTinput_JVN6_S22_R1_001.fastq.gz, SRR12539918, SRR12539918.lite SRA
SRX9029619 SRP279200 RNA-Seq SINGLE
GSM4751656: Tsc Met input; Mus musculus; RNA-Seq
Sample: SRS7280900
BioProject: PRJNA659995
BioSample: SAMN15931736
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Tsc2 fl/fl; AlbCreEr
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: 2mM Metformin for 2h serum-starved
insulin treatment: Untreated
rna fraction: polyA RNA
Original files (1)
Primary Hepatocytes
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR12539919 20336154 1037143854 337.06 TscMetinput_JVN7_S23_R1_001.fastq.gz, SRR12539919, SRR12539919.lite SRA
SRX9029620 SRP279200 RNA-Seq SINGLE
GSM4751657: Tsc Ins input rep1; Mus musculus; RNA-Seq
Sample: SRS7280902
BioProject: PRJNA659995
BioSample: SAMN15931735
Platform: ILLUMINA
Instrument: NextSeq 500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Tsc2 fl/fl; AlbCreEr
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: untreated - serum starved
insulin treatment: 15 minute pre-treatment with 1nM Insulin
rna fraction: polyA RNA
Original files (1)
Primary Hepatocytes
Runs (2)
Run Spots Bases Size (MB) Files Link
SRR12539920 8575644 428782200 131.62 tsc_ins_input_JVN_66_S16_R1_001.fastq.gz, SRR12539920, SRR12539920.li… SRA
SRR12539921 23687731 1208074281 381.47 tsc_ins_input_JVN_66_S16_R1_001.fastq-2.gz, SRR12539921, SRR12539921.… SRA
SRX9029621 SRP279200 RNA-Seq SINGLE
GSM4751658: Tsc Ins input rep2; Mus musculus; RNA-Seq
Sample: SRS7280903
BioProject: PRJNA659995
BioSample: SAMN15931734
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Tsc2 fl/fl; AlbCreEr
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: untreated - serum starved
insulin treatment: 15 minute pre-treatment with 1nM Insulin
rna fraction: polyA RNA
Original files (1)
Primary Hepatocytes
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR12539922 22222562 1133350662 373.67 TscInsInput_JVN17_S33_R1_001.fastq.gz, SRR12539922, SRR12539922.lite SRA
SRX9029622 SRP279200 RNA-Seq SINGLE
GSM4751659: Tsc InsMet input rep1; Mus musculus; RNA-Seq
Sample: SRS7280904
BioProject: PRJNA659995
BioSample: SAMN15931733
Platform: ILLUMINA
Instrument: NextSeq 500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Tsc2 fl/fl; AlbCreEr
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: 2mM Metformin for 2h serum-starved
insulin treatment: 15 minute pre-treatment with 1nM Insulin
rna fraction: polyA RNA
Original files (1)
Primary Hepatocytes
Runs (2)
Run Spots Bases Size (MB) Files Link
SRR12539923 8569539 428476950 132.1 tsc_ins_met_input_JVN_67_S17_R1_001.fastq.gz, SRR12539923, SRR1253992… SRA
SRR12539924 24111405 1229681655 390.06 tsc_ins_met_input_JVN_67_S17_R1_001.fastq-2.gz, SRR12539924, SRR12539… SRA
SRX9029623 SRP279200 RNA-Seq SINGLE
GSM4751660: Tsc InsMet input rep2; Mus musculus; RNA-Seq
Sample: SRS7280905
BioProject: PRJNA659995
BioSample: SAMN15931732
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Tsc2 fl/fl; AlbCreEr
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: 2mM Metformin for 2h serum-starved
insulin treatment: 15 minute pre-treatment with 1nM Insulin
rna fraction: polyA RNA
Original files (1)
Primary Hepatocytes
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR12539925 20087257 1024450107 341.46 TscInsMetInput_JVN18_S34_R1_001.fastq.gz, SRR12539925, SRR12539925.li… SRA
SRX9029624 SRP279200 RNA-Seq SINGLE
GSM4751661: AATsc UT input; Mus musculus; RNA-Seq
Sample: SRS7280906
BioProject: PRJNA659995
BioSample: SAMN15931731
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Raptor - S722A; S792A; Tsc2 fl/fl; AlbCreEr
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: untreated - serum starved
insulin treatment: Untreated
rna fraction: polyA RNA
Original files (1)
Primary Hepatocytes
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR12539926 21041503 1073116653 349.58 AATscUTInput_JVN8_S24_R1_001.fastq.gz, SRR12539926, SRR12539926.lite SRA
SRX9029625 SRP279200 RNA-Seq SINGLE
GSM4751662: AATsc Met input; Mus musculus; RNA-Seq
Sample: SRS7280908
BioProject: PRJNA659995
BioSample: SAMN15931730
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Raptor - S722A; S792A; Tsc2 fl/fl; AlbCreEr
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: 2mM Metformin for 2h serum-starved
insulin treatment: Untreated
rna fraction: polyA RNA
Original files (1)
Primary Hepatocytes
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR12539927 17207713 877593363 350.9 AATscMetInput_JVN9_S25_R1_001.fastq.gz, SRR12539927, SRR12539927.lite SRA
SRX9029626 SRP279200 RNA-Seq SINGLE
GSM4751663: AATsc Ins input rep1; Mus musculus; RNA-Seq
Sample: SRS7280907
BioProject: PRJNA659995
BioSample: SAMN15931729
Platform: ILLUMINA
Instrument: NextSeq 500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Raptor - S722A; S792A; Tsc2 fl/fl; AlbCreEr
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: untreated - serum starved
insulin treatment: 15 minute pre-treatment with 1nM Insulin
rna fraction: polyA RNA
Original files (1)
Primary Hepatocytes
Runs (2)
Run Spots Bases Size (MB) Files Link
SRR12539928 8009047 400452350 121.87 aa_tsc_ins_input_JVN_68_S18_R1_001.fastq.gz, SRR12539928, SRR12539928… SRA
SRR12539929 22205319 1132471269 356.64 aa_tsc_ins_input_JVN_68_S18_R1_001.fastq-2.gz, SRR12539929, SRR125399… SRA
SRX9029627 SRP279200 RNA-Seq SINGLE
GSM4751664: AATsc Ins input rep2; Mus musculus; RNA-Seq
Sample: SRS7280909
BioProject: PRJNA659995
BioSample: SAMN15931636
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Raptor - S722A; S792A; Tsc2 fl/fl; AlbCreEr
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: untreated - serum starved
insulin treatment: 15 minute pre-treatment with 1nM Insulin
rna fraction: polyA RNA
Original files (1)
Primary Hepatocytes
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR12539930 23476078 1197279978 393.58 AATSCInsInput_JVN19_S35_R1_001.fastq.gz, SRR12539930, SRR12539930.lite SRA
SRX9029628 SRP279200 RNA-Seq SINGLE
GSM4751665: AATsc InsMet input rep1; Mus musculus; RNA-Seq
Sample: SRS7280910
BioProject: PRJNA659995
BioSample: SAMN15931635
Platform: ILLUMINA
Instrument: NextSeq 500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Raptor - S722A; S792A; Tsc2 fl/fl; AlbCreEr
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: 2mM Metformin for 2h serum-starved
insulin treatment: 15 minute pre-treatment with 1nM Insulin
rna fraction: polyA RNA
Original files (1)
Primary Hepatocytes
Runs (2)
Run Spots Bases Size (MB) Files Link
SRR12539931 7406042 370302100 113.54 aa_tsc_ins_met_input_JVN_69_S19_R1_001.fastq.gz, SRR12539931, SRR1253… SRA
SRR12539932 20467890 1043862390 330.49 aa_tsc_ins_met_input_JVN_69_S19_R1_001.fastq-2.gz, SRR12539932, SRR12… SRA
SRX9029629 SRP279200 RNA-Seq SINGLE
GSM4751666: AATsc InsMet input rep2; Mus musculus; RNA-Seq
Sample: SRS7280911
BioProject: PRJNA659995
BioSample: SAMN15931634
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Raptor - S722A; S792A; Tsc2 fl/fl; AlbCreEr
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: 2mM Metformin for 2h serum-starved
insulin treatment: 15 minute pre-treatment with 1nM Insulin
rna fraction: polyA RNA
Original files (1)
Primary Hepatocytes
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR12539933 14670192 748179792 304.36 AATscInsMetInput_JVN20_S36_R1_001.fastq.gz, SRR12539933, SRR12539933.… SRA
SRX9029630 SRP279200 OTHER SINGLE
GSM4751691: Tsc Ins poly rep2; Mus musculus; OTHER
Sample: SRS7280912
BioProject: PRJNA659995
BioSample: SAMN15931705
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Tsc2 fl/fl; AlbCreEr
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: untreated - serum starved
insulin treatment: 15 minute pre-treatment with 1nM Insulin
rna fraction: polysome fractionated samples
Original files (1)
Primary Hepatocytes
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR12539966 21906644 1117238844 357.42 TscIns_JVN35_S51_R1_001.fastq.gz, SRR12539966, SRR12539966.lite SRA
SRX9029631 SRP279200 OTHER SINGLE
GSM4751692: Tsc InsMet poly rep1; Mus musculus; OTHER
Sample: SRS7280913
BioProject: PRJNA659995
BioSample: SAMN15931703
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Tsc2 fl/fl; AlbCreEr
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: 2mM Metformin for 2h serum-starved
insulin treatment: 15 minute pre-treatment with 1nM Insulin
rna fraction: polysome fractionated samples
Original files (1)
Primary Hepatocytes
Runs (2)
Run Spots Bases Size (MB) Files Link
SRR12539967 10595155 540352905 153.46 tsc_ins_met_JVN_56_S6_R1_001.fastq.gz, SRR12539967, SRR12539967.lite SRA
SRR12539968 23434177 1171708850 364.97 tsc_ins_met_JVN_56_S6_R1_001.fastq-2.gz, SRR12539968, SRR12539968.lite SRA
SRX9029632 SRP279200 OTHER SINGLE
GSM4751693: Tsc InsMet poly rep2; Mus musculus; OTHER
Sample: SRS7280915
BioProject: PRJNA659995
BioSample: SAMN15931633
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Tsc2 fl/fl; AlbCreEr
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: 2mM Metformin for 2h serum-starved
insulin treatment: 15 minute pre-treatment with 1nM Insulin
rna fraction: polysome fractionated samples
Original files (1)
Primary Hepatocytes
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR12539969 23319625 1189300875 391.53 TscInsMet_JVN36_S52_R1_001.fastq.gz, SRR12539969, SRR12539969.lite SRA
SRX9029633 SRP279200 OTHER SINGLE
GSM4751694: AATsc UT poly; Mus musculus; OTHER
Sample: SRS7280914
BioProject: PRJNA659995
BioSample: SAMN15931632
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Raptor - S722A; S792A; Tsc2 fl/fl; AlbCreEr
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: untreated - serum starved
insulin treatment: Untreated
rna fraction: polysome fractionated samples
Original files (1)
Primary Hepatocytes
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR12539970 24552631 1252184181 399.92 AATscUT_JVN37_S53_R1_001.fastq.gz, SRR12539970, SRR12539970.lite SRA
SRX9029634 SRP279200 OTHER SINGLE
GSM4751695: AATsc Met poly; Mus musculus; OTHER
Sample: SRS7280918
BioProject: PRJNA659995
BioSample: SAMN15931681
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Raptor - S722A; S792A; Tsc2 fl/fl; AlbCreEr
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: 2mM Metformin for 2h serum-starved
insulin treatment: Untreated
rna fraction: polysome fractionated samples
Original files (1)
Primary Hepatocytes
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR12539971 25585360 1304853360 415.99 AATscMet_JVN38_S54_R1_001.fastq.gz, SRR12539971, SRR12539971.lite SRA
SRX9029635 SRP279200 OTHER SINGLE
GSM4751696: AATsc Ins poly rep1; Mus musculus; OTHER
Sample: SRS7280917
BioProject: PRJNA659995
BioSample: SAMN15931680
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Raptor - S722A; S792A; Tsc2 fl/fl; AlbCreEr
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: untreated - serum starved
insulin treatment: 15 minute pre-treatment with 1nM Insulin
rna fraction: polysome fractionated samples
Original files (1)
Primary Hepatocytes
Runs (2)
Run Spots Bases Size (MB) Files Link
SRR12539972 8086304 412401504 118.92 aa_tsc_ins_JVN_57_S7_R1_001.fastq.gz, SRR12539972, SRR12539972.lite SRA
SRR12539973 17745494 887274700 275.01 aa_tsc_ins_JVN_57_S7_R1_001.fastq-2.gz, SRR12539973, SRR12539973.lite SRA
SRX9029636 SRP279200 OTHER SINGLE
GSM4751697: AATsc Ins poly rep2; Mus musculus; OTHER
Sample: SRS7280916
BioProject: PRJNA659995
BioSample: SAMN15931679
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Raptor - S722A; S792A; Tsc2 fl/fl; AlbCreEr
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: untreated - serum starved
insulin treatment: 15 minute pre-treatment with 1nM Insulin
rna fraction: polysome fractionated samples
Original files (1)
Primary Hepatocytes
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR12539974 24126104 1230431304 391.56 AATSCIns_JVN39_S55_R1_001.fastq.gz, SRR12539974, SRR12539974.lite SRA
SRX9029637 SRP279200 OTHER SINGLE
GSM4751698: AATsc InsMet poly rep1; Mus musculus; OTHER
Sample: SRS7280919
BioProject: PRJNA659995
BioSample: SAMN15931678
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Raptor - S722A; S792A; Tsc2 fl/fl; AlbCreEr
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: 2mM Metformin for 2h serum-starved
insulin treatment: 15 minute pre-treatment with 1nM Insulin
rna fraction: polysome fractionated samples
Original files (1)
Primary Hepatocytes
Runs (2)
Run Spots Bases Size (MB) Files Link
SRR12539975 11253462 573926562 166.79 aa_tsc_ins__met_JVN_58_S8_R1_001.fastq.gz, SRR12539975, SRR12539975.l… SRA
SRR12539976 23820809 1191040450 370.87 aa_tsc_ins__met_JVN_58_S8_R1_001.fastq-2.gz, SRR12539976, SRR12539976… SRA
SRX9029638 SRP279200 OTHER SINGLE
GSM4751699: AATsc InsMet poly rep2; Mus musculus; OTHER
Sample: SRS7280920
BioProject: PRJNA659995
BioSample: SAMN15931677
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Raptor - S722A; S792A; Tsc2 fl/fl; AlbCreEr
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: 2mM Metformin for 2h serum-starved
insulin treatment: 15 minute pre-treatment with 1nM Insulin
rna fraction: polysome fractionated samples
Original files (1)
Primary Hepatocytes
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR12539977 22812115 1163417865 372.09 AATscInsMet_JVN40_S56_R1_001.fastq.gz, SRR12539977, SRR12539977.lite SRA
SRX9029639 SRP279200 OTHER SINGLE
GSM4751700: Ampk UT poly; Mus musculus; OTHER
Sample: SRS7280921
BioProject: PRJNA659995
BioSample: SAMN15931676
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Ampka1 fl/fl; Ampka2 fl/fl; AlbCreER
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: untreated - serum starved
insulin treatment: Untreated
rna fraction: polysome fractionated samples
Original files (1)
Primary Hepatocytes
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR12539978 24564240 1252776240 400.47 AMPKUT_JVN41_S57_R1_001.fastq.gz, SRR12539978, SRR12539978.lite SRA
SRX9029640 SRP279200 OTHER SINGLE
GSM4751701: Ampk Met poly; Mus musculus; OTHER
Sample: SRS7280922
BioProject: PRJNA659995
BioSample: SAMN15931675
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Ampka1 fl/fl; Ampka2 fl/fl; AlbCreER
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: 2mM Metformin for 2h serum-starved
insulin treatment: Untreated
rna fraction: polysome fractionated samples
Original files (1)
Primary Hepatocytes
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR12539979 25319860 1291312860 414.01 AMPKMet_JVN42_S58_R1_001.fastq.gz, SRR12539979, SRR12539979.lite SRA
SRX9029641 SRP279200 OTHER SINGLE
GSM4751702: Ampk Ins poly rep1; Mus musculus; OTHER
Sample: SRS7280923
BioProject: PRJNA659995
BioSample: SAMN15931674
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Ampka1 fl/fl; Ampka2 fl/fl; AlbCreER
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: untreated - serum starved
insulin treatment: 15 minute pre-treatment with 1nM Insulin
rna fraction: polysome fractionated samples
Original files (1)
Primary Hepatocytes
Runs (2)
Run Spots Bases Size (MB) Files Link
SRR12539980 2619543 133596693 57.39 ampk_ins_JVN_59_S9_R1_001.fastq.gz, SRR12539980, SRR12539980.lite SRA
SRR12539981 21110659 1055532950 332.44 ampk_ins_JVN_59_S9_R1_001.fastq-2.gz, SRR12539981, SRR12539981.lite SRA
SRX9029642 SRP279200 OTHER SINGLE
GSM4751703: Ampk Ins poly rep2; Mus musculus; OTHER
Sample: SRS7280924
BioProject: PRJNA659995
BioSample: SAMN15931673
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Ampka1 fl/fl; Ampka2 fl/fl; AlbCreER
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: untreated - serum starved
insulin treatment: 15 minute pre-treatment with 1nM Insulin
rna fraction: polysome fractionated samples
Original files (1)
Primary Hepatocytes
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR12539982 26466934 1349813634 433.63 AMPKIns_JVN43_S59_R1_001.fastq.gz, SRR12539982, SRR12539982.lite SRA
SRX9029643 SRP279200 OTHER SINGLE
GSM4751704: Ampk InsMet poly rep1; Mus musculus; OTHER
Sample: SRS7280925
BioProject: PRJNA659995
BioSample: SAMN15931672
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Ampka1 fl/fl; Ampka2 fl/fl; AlbCreER
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: 2mM Metformin for 2h serum-starved
insulin treatment: 15 minute pre-treatment with 1nM Insulin
rna fraction: polysome fractionated samples
Original files (1)
Primary Hepatocytes
Runs (2)
Run Spots Bases Size (MB) Files Link
SRR12539983 2755746 140543046 60.54 ampk_ins_met_JVN_60_S10_R1_001.fastq.gz, SRR12539983, SRR12539983.lite SRA
SRR12539984 23767534 1188376700 374.94 ampk_ins_met_JVN_60_S10_R1_001.fastq-2.gz, SRR12539984, SRR12539984.l… SRA
SRX9029644 SRP279200 OTHER SINGLE
GSM4751705: Ampk InsMet poly rep2; Mus musculus; OTHER
Sample: SRS7280926
BioProject: PRJNA659995
BioSample: SAMN15931671
Platform: ILLUMINA
Instrument: Illumina HiSeq 2500
Organism: Mus musculus
Sample attributes
source_name: Primary Hepatocytes
strain: C57BL/6 (F3 generation)
tissue: primary hepatocytes
age: 8-16 weeks old
genotype: Ampka1 fl/fl; Ampka2 fl/fl; AlbCreER
tamoxifen treatment: Tamoxifen treatment 3x 14-17 days prior to hepatocyte isolation
metformin treatment: 2mM Metformin for 2h serum-starved
insulin treatment: 15 minute pre-treatment with 1nM Insulin
rna fraction: polysome fractionated samples
Original files (1)
Primary Hepatocytes
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR12539985 22870608 1166401008 382.84 AMPKInsMet_JVN44_S60_R1_001.fastq.gz, SRR12539985, SRR12539985.lite SRA

Linked Publications (1)

AMPK regulation of Raptor and TSC2 mediate metformin effects on transcriptional control of anabolism and inflammation.
Genes & development · 2020

Data Files (88)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
— AAIns_JVN31_S47_R1_001.fastq.gz OTHER 408.8 MB link
— aa_ins_JVN_53_S3_R1_001.fastq-2.gz OTHER 345.9 MB link
— aa_ins_JVN_53_S3_R1_001.fastq.gz OTHER 148.5 MB link
— AAInsMet_JVN32_S48_R1_001.fastq.gz OTHER 429.8 MB link
— aa_ins_met_JVN_54_S4_R1_001.fastq-2.gz OTHER 330.7 MB link
— aa_ins_met_JVN_54_S4_R1_001.fastq.gz OTHER 143.2 MB link
— AAMet_JVN30_S46_R1_001.fastq.gz OTHER 450.6 MB link
— AATSCIns_JVN39_S55_R1_001.fastq.gz OTHER 391.6 MB link
— aa_tsc_ins_JVN_57_S7_R1_001.fastq-2.gz OTHER 275.0 MB link
— aa_tsc_ins_JVN_57_S7_R1_001.fastq.gz OTHER 118.9 MB link
— AATscInsMet_JVN40_S56_R1_001.fastq.gz OTHER 372.1 MB link
— aa_tsc_ins__met_JVN_58_S8_R1_001.fastq-2.gz OTHER 370.9 MB link
— aa_tsc_ins__met_JVN_58_S8_R1_001.fastq.gz OTHER 166.8 MB link
— AATscMet_JVN38_S54_R1_001.fastq.gz OTHER 416.0 MB link
— AATscUT_JVN37_S53_R1_001.fastq.gz OTHER 399.9 MB link
— AAUT_JVN29_S45_R1_001.fastq.gz OTHER 452.6 MB link
— AMPKIns_JVN43_S59_R1_001.fastq.gz OTHER 433.6 MB link
— ampk_ins_JVN_59_S9_R1_001.fastq-2.gz OTHER 332.4 MB link
— ampk_ins_JVN_59_S9_R1_001.fastq.gz OTHER 57.4 MB link
— AMPKInsMet_JVN44_S60_R1_001.fastq.gz OTHER 382.8 MB link
— ampk_ins_met_JVN_60_S10_R1_001.fastq-2.gz OTHER 374.9 MB link
— ampk_ins_met_JVN_60_S10_R1_001.fastq.gz OTHER 60.5 MB link
— AMPKMet_JVN42_S58_R1_001.fastq.gz OTHER 414.0 MB link
— AMPKUT_JVN41_S57_R1_001.fastq.gz OTHER 400.5 MB link
— TscIns_JVN35_S51_R1_001.fastq.gz OTHER 357.4 MB link
— tsc_ins_JVN_55_S5_R1_001.fastq-2.gz OTHER 324.7 MB link
— tsc_ins_JVN_55_S5_R1_001.fastq.gz OTHER 138.9 MB link
— TscInsMet_JVN36_S52_R1_001.fastq.gz OTHER 391.5 MB link
— tsc_ins_met_JVN_56_S6_R1_001.fastq-2.gz OTHER 365.0 MB link
— tsc_ins_met_JVN_56_S6_R1_001.fastq.gz OTHER 153.5 MB link
— TscMet_JVN34_S50_R1_001.fastq.gz OTHER 391.1 MB link
— TscUT_JVN33_S49_R1_001.fastq.gz OTHER 415.7 MB link
— WtInk_JVN25_S41_R1_001.fastq.gz OTHER 419.7 MB link
— wt_ink_JVN_61_S11_R1_001.fastq-2.gz OTHER 378.1 MB link
— wt_ink_JVN_61_S11_R1_001.fastq.gz OTHER 64.9 MB link
— WTInsInk_JVN28_S44_R1_001.fastq.gz OTHER 411.5 MB link
— WTIns_JVN26_S42_R1_001.fastq.gz OTHER 391.5 MB link
— wt_ins_JVN_51_S1_R1_001.fastq-2.gz OTHER 449.5 MB link
— wt_ins_JVN_51_S1_R1_001.fastq.gz OTHER 195.5 MB link
— WTInsMet_JVN27_S43_R1_001.fastq.gz OTHER 456.4 MB link
— wt_ins_met_JVN_52_S2_R1_001.fastq-2.gz OTHER 636.3 MB link
— wt_ins_met_JVN_52_S2_R1_001.fastq.gz OTHER 297.1 MB link
— WTMet_JVN24_S40_R1_001.fastq.gz OTHER 385.5 MB link
— AAInsInput_JVN15_S31_R1_001.fastq.gz RNA-Seq 361.5 MB link
— aa_ins_input_JVN_64_S14_R1_001.fastq-2.gz RNA-Seq 373.2 MB link
— aa_ins_input_JVN_64_S14_R1_001.fastq.gz RNA-Seq 130.0 MB link
— AAInsMetInput_JVN16_S32_R1_001.fastq.gz RNA-Seq 349.8 MB link
— aa_ins_met_input_JVN_65_S15_R1_001.fastq-2.gz RNA-Seq 327.3 MB link
— aa_ins_met_input_JVN_65_S15_R1_001.fastq.gz RNA-Seq 111.9 MB link
— AAMetinput_JVN5_S21_R1_001.fastq.gz RNA-Seq 334.7 MB link
— AATSCInsInput_JVN19_S35_R1_001.fastq.gz RNA-Seq 393.6 MB link
— aa_tsc_ins_input_JVN_68_S18_R1_001.fastq-2.gz RNA-Seq 356.6 MB link
— aa_tsc_ins_input_JVN_68_S18_R1_001.fastq.gz RNA-Seq 121.9 MB link
— AATscInsMetInput_JVN20_S36_R1_001.fastq.gz RNA-Seq 304.4 MB link
— aa_tsc_ins_met_input_JVN_69_S19_R1_001.fastq-2.gz RNA-Seq 330.5 MB link
— aa_tsc_ins_met_input_JVN_69_S19_R1_001.fastq.gz RNA-Seq 113.5 MB link
— AATscMetInput_JVN9_S25_R1_001.fastq.gz RNA-Seq 350.9 MB link
— AATscUTInput_JVN8_S24_R1_001.fastq.gz RNA-Seq 349.6 MB link
— AAUTinput_JVN4_S20_R1_001.fastq.gz RNA-Seq 330.6 MB link
— AMPKInsInput_JVN21_S37_R1_001.fastq.gz RNA-Seq 373.0 MB link
— ampk_ins_input_JVN_70_S20_R1_001.fastq-2.gz RNA-Seq 356.1 MB link
— ampk_ins_input_JVN_70_S20_R1_001.fastq.gz RNA-Seq 126.1 MB link
— AMPKInsMetInput_JVN22_S38_R1_001.fastq.gz RNA-Seq 330.2 MB link
— ampk_ins_met_input_JVN_71_S21_R1_001.fastq-2.gz RNA-Seq 312.1 MB link
— ampk_ins_met_input_JVN_71_S21_R1_001.fastq.gz RNA-Seq 109.0 MB link
— AMPKMetInput_JVN11_S27_R1_001.fastq.gz RNA-Seq 390.5 MB link
— AMPKUTInput_JVN10_S26_R1_001.fastq.gz RNA-Seq 379.2 MB link
— TscInsInput_JVN17_S33_R1_001.fastq.gz RNA-Seq 373.7 MB link
— tsc_ins_input_JVN_66_S16_R1_001.fastq-2.gz RNA-Seq 381.5 MB link
— tsc_ins_input_JVN_66_S16_R1_001.fastq.gz RNA-Seq 131.6 MB link
— TscInsMetInput_JVN18_S34_R1_001.fastq.gz RNA-Seq 341.5 MB link
— tsc_ins_met_input_JVN_67_S17_R1_001.fastq-2.gz RNA-Seq 390.1 MB link
— tsc_ins_met_input_JVN_67_S17_R1_001.fastq.gz RNA-Seq 132.1 MB link
— TscMetinput_JVN7_S23_R1_001.fastq.gz RNA-Seq 337.1 MB link
— TscUTinput_JVN6_S22_R1_001.fastq.gz RNA-Seq 344.9 MB link
— WtInkinput_JVN3_S19_R1_001.fastq.gz RNA-Seq 336.4 MB link
— wt_ink_input_JVN_72_S22_R1_001.fastq-2.gz RNA-Seq 302.2 MB link
— wt_ink_input_JVN_72_S22_R1_001.fastq.gz RNA-Seq 107.1 MB link
— WTInsInkInput_JVN14_S30_R1_001.fastq.gz RNA-Seq 328.5 MB link
— WTInsInput_JVN12_S28_R1_001.fastq.gz RNA-Seq 395.5 MB link
— wt_ins_input_JVN_62_S12_R1_001.fastq-2.gz RNA-Seq 194.6 MB link
— wt_ins_input_JVN_62_S12_R1_001.fastq.gz RNA-Seq 127.8 MB link
— WTInsMetInput_JVN13_S29_R1_001.fastq.gz RNA-Seq 349.5 MB link
— wt_ins_met_input_JVN_63_S13_R1_001.fastq-2.gz RNA-Seq 338.2 MB link
— wt_ins_met_input_JVN_63_S13_R1_001.fastq.gz RNA-Seq 116.6 MB link
— WTMetinput_JVN2_S18_R1_001.fastq.gz RNA-Seq 336.6 MB link
— WTUTinput_JVN1_S17_R1_001.fastq.gz RNA-Seq 346.0 MB link
— WTUT_JVN23_S39_R1_001.fastq.gz RNA-Seq 401.5 MB link