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GSE64078

GSE GEO
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ALS-causative mutations in FUS/TLS confer gain- and loss-of-function by altered association with SMN and U1-snRNP

Organism: Homo sapiens
Platform: GPL11154
Samples: 49
Experiment Types:
Expression profiling by high throughput sequencing
Submitted: Dec 11 2014
Last Updated: May 15 2019
Status: Public on Feb 10 2015
Contact: Xiang-Dong,,Fu (UC San Diego)

Relations

BioProject: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA270070 SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRP051107

Summary

The RNA-binding protein FUS/TLS, mutation in which is causative of the fatal motor neuron disease ALS, is demonstrated to directly bind to the U1-snRNP and SMN complexes. ALS-causative mutations in FUS/TLS are shown to abnormally enhance their interaction with SMN and reduce interaction with U1-snRNP. Correspondingly, global RNA analysis reveals a mutant-dependent loss of splicing activity, with ALS-linked mutants failing to reverse changes caused by loss of wild-type FUS/TLS. Furthermore, a common FUS/TLS mutant-associated RNA splicing signature is identified in ALS patient fibroblasts. Taken together, our studies establish potentially converging disease mechanisms in ALS and spinal muscular atrophy, with ALS-causative mutants acquiring properties representing both gain (dysregulation of SMN) and loss (reduced RNA processing mediated by U1-snRNP) of function.

Overall Design

RNA-mediated oligonucleotide Annealing, Selection, and Ligation with Next-Generation sequencing (RASL-seq) method was used for analyzing alternative splicing changes. Oligonucleotide probes are designed to anneal to the exon-exon junctions. The probe library was assembled to assess 5530 unique alternative splicing events, most of which were exon inclusion or skipping, with a minority for alternative 5’- or 3’- splice sites. The splicing changes were compared among groups of reducing FUS/TLS or SMN levels, or expressing various FUS mutations to determine the loss versus gain of FUS/TLS function on splicing regulation.

Analysis (1 step)

View Data Processing
Processing steps for GSE64078
  1. The sequenced 40 bases were mapped to the oligo pool sequences with Bowtie by allowing 1 mismatch in each of the donor and acceptor sequences.

Supplementary Files (1)

GSE64078_RAW.tar Download
GEO Samples (49)
GSM1563897 GSM1563898 GSM1563899 GSM1563900 GSM1563901 GSM1563902 GSM1563903 GSM1563904 GSM1563905 GSM1563906 GSM1563907 GSM1563908 GSM1563909 GSM1563910 GSM1563911 GSM1563912 GSM1563913 GSM1563914 GSM1563915 GSM1563916 GSM1563917 GSM1563918 GSM1563919 GSM1563920 GSM1563921 GSM1563922 GSM1563923 GSM1563924 GSM1563925 GSM1563926 GSM1563927 GSM1563928 GSM1563929 GSM1563930 GSM1563931 GSM1563932 GSM1563933 GSM1563934 GSM1563935 GSM1563936 GSM1563937 GSM1563938 GSM1563939 GSM1563940 GSM1563941 GSM1563942 GSM1563943 GSM1563944 GSM1563945

Dataset Citations (1)

ALS-causative mutations in FUS/TLS confer gain and loss of function by altered association with SMN and U1-snRNP.
PMID 25625564 · 2015 · Nature communications
Shuying Sun, Shuo-Chien Ling, Jinsong Qiu, Claudio P Albuquerque, Yu Zhou, Seiya Tokunaga, Hairi Li, Haiyan Qiu, Anh Bui, Gene W Yeo, Eric J Huang, Kevin Eggan, Huilin Zhou, Xiang-Dong Fu, Clotilde Lagier-Tourenne, Don W Cleveland

SRA Experiments (49) and Runs (49)

Total: 7799 MB
SRX807449 SRP051107 OTHER SINGLE
GSM1563897: FLP-IN_parental_1; Homo sapiens; OTHER
Sample: SRS789053
BioProject: PRJNA270070
BioSample: SAMN03262695
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: HeLa
cell type: human cervical cancer patient imortalized
strategy: RASL-seq
cell line: HeLa
sample type: FLP-IN HeLa parental
Original files (1)
HeLa
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708193 4903012 196120480 151.56 FLP-IN_parental_1.fastq.gz, SRR1708193, SRR1708193.sralite SRA
SRX807450 SRP051107 OTHER SINGLE
GSM1563898: FLP-IN_parental_2; Homo sapiens; OTHER
Sample: SRS789054
BioProject: PRJNA270070
BioSample: SAMN03262688
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: HeLa
cell type: human cervical cancer patient imortalized
strategy: RASL-seq
cell line: HeLa
sample type: FLP-IN HeLa parental
Original files (1)
HeLa
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708194 5527371 221094840 173.48 FLP-IN_parental_2.fastq.gz, SRR1708194, SRR1708194.sralite SRA
SRX807451 SRP051107 OTHER SINGLE
GSM1563899: FLP-IN_parental_3; Homo sapiens; OTHER
Sample: SRS789055
BioProject: PRJNA270070
BioSample: SAMN03262664
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: HeLa
cell type: human cervical cancer patient imortalized
strategy: RASL-seq
cell line: HeLa
sample type: FLP-IN HeLa parental
Original files (1)
HeLa
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708195 6259627 250385080 195.44 FLP-IN_parental_3.fastq.gz, SRR1708195, SRR1708195.sralite SRA
SRX807452 SRP051107 OTHER SINGLE
GSM1563900: FLP-IN_FUS-RNAi_1; Homo sapiens; OTHER
Sample: SRS789056
BioProject: PRJNA270070
BioSample: SAMN03262705
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: HeLa
cell type: human cervical cancer patient imortalized
strategy: RASL-seq
cell line: HeLa
sample type: FLP-IN HeLa parental + FUS-RNAi
Original files (1)
HeLa
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708196 4321682 172867280 135.66 FLP-IN_FUS-RNAi_1.fastq.gz, SRR1708196, SRR1708196.sralite SRA
SRX807453 SRP051107 OTHER SINGLE
GSM1563901: FLP-IN_FUS-RNAi_2; Homo sapiens; OTHER
Sample: SRS789057
BioProject: PRJNA270070
BioSample: SAMN03262672
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: HeLa
cell type: human cervical cancer patient imortalized
strategy: RASL-seq
cell line: HeLa
sample type: FLP-IN HeLa parental + FUS-RNAi
Original files (1)
HeLa
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708197 4363315 174532600 137.16 FLP-IN_FUS-RNAi_2.fastq.gz, SRR1708197, SRR1708197.sralite SRA
SRX807454 SRP051107 OTHER SINGLE
GSM1563902: FLP-IN_FUS-RNAi_3; Homo sapiens; OTHER
Sample: SRS789058
BioProject: PRJNA270070
BioSample: SAMN03262683
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: HeLa
cell type: human cervical cancer patient imortalized
strategy: RASL-seq
cell line: HeLa
sample type: FLP-IN HeLa parental + FUS-RNAi
Original files (1)
HeLa
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708198 3923191 156927640 122.5 FLP-IN_FUS-RNAi_3.fastq.gz, SRR1708198, SRR1708198.sralite SRA
SRX807455 SRP051107 OTHER SINGLE
GSM1563903: FLP-IN_WT-FUS_FUS-RNAi_1; Homo sapiens; OTHER
Sample: SRS789059
BioProject: PRJNA270070
BioSample: SAMN03262703
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: HeLa
cell type: human cervical cancer patient imortalized
strategy: RASL-seq
cell line: HeLa
sample type: wt-FUS + FUS-RNAi
Original files (1)
HeLa
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708199 5599326 223973040 175.59 FLP-IN_WT-FUS_FUS-RNAi_1.fastq.gz, SRR1708199, SRR1708199.sralite SRA
SRX807456 SRP051107 OTHER SINGLE
GSM1563904: FLP-IN_WT-FUS_FUS-RNAi_2; Homo sapiens; OTHER
Sample: SRS789060
BioProject: PRJNA270070
BioSample: SAMN03262676
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: HeLa
cell type: human cervical cancer patient imortalized
strategy: RASL-seq
cell line: HeLa
sample type: wt-FUS + FUS-RNAi
Original files (1)
HeLa
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708200 1872861 74914440 59.36 FLP-IN_WT-FUS_FUS-RNAi_2.fastq.gz, SRR1708200, SRR1708200.sralite SRA
SRX807457 SRP051107 OTHER SINGLE
GSM1563905: FLP-IN_WT-FUS_FUS-RNAi_3; Homo sapiens; OTHER
Sample: SRS789061
BioProject: PRJNA270070
BioSample: SAMN03262673
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: HeLa
cell type: human cervical cancer patient imortalized
strategy: RASL-seq
cell line: HeLa
sample type: wt-FUS + FUS-RNAi
Original files (1)
HeLa
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708201 5158504 206340160 162.27 FLP-IN_WT-FUS_FUS-RNAi_3.fastq.gz, SRR1708201, SRR1708201.sralite SRA
SRX807458 SRP051107 OTHER SINGLE
GSM1563906: FLP-IN_R495X_FUS-RNAi_1; Homo sapiens; OTHER
Sample: SRS789062
BioProject: PRJNA270070
BioSample: SAMN03262692
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: HeLa
cell type: human cervical cancer patient imortalized
strategy: RASL-seq
cell line: HeLa
sample type: R495X + FUS-RNAi
Original files (1)
HeLa
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708202 7663177 306527080 242.66 FLP-IN_R495X_FUS-RNAi_1.fastq.gz, SRR1708202, SRR1708202.sralite SRA
SRX807459 SRP051107 OTHER SINGLE
GSM1563907: FLP-IN_R495X_FUS-RNAi_2; Homo sapiens; OTHER
Sample: SRS789063
BioProject: PRJNA270070
BioSample: SAMN03262691
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: HeLa
cell type: human cervical cancer patient imortalized
strategy: RASL-seq
cell line: HeLa
sample type: R495X + FUS-RNAi
Original files (1)
HeLa
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708203 5960935 238437400 186.61 FLP-IN_R495X_FUS-RNAi_2.fastq.gz, SRR1708203, SRR1708203.sralite SRA
SRX807460 SRP051107 OTHER SINGLE
GSM1563908: FLP-IN_R495X_FUS-RNAi_3; Homo sapiens; OTHER
Sample: SRS789066
BioProject: PRJNA270070
BioSample: SAMN03262694
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: HeLa
cell type: human cervical cancer patient imortalized
strategy: RASL-seq
cell line: HeLa
sample type: R495X + FUS-RNAi
Original files (1)
HeLa
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708204 6771302 270852080 213.38 FLP-IN_R495X_FUS-RNAi_3.fastq.gz, SRR1708204, SRR1708204.sralite SRA
SRX807461 SRP051107 OTHER SINGLE
GSM1563909: FLP-IN_R514G_FUS-RNAi_1; Homo sapiens; OTHER
Sample: SRS789064
BioProject: PRJNA270070
BioSample: SAMN03262696
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: HeLa
cell type: human cervical cancer patient imortalized
strategy: RASL-seq
cell line: HeLa
sample type: R514G + FUS-RNAi
Original files (1)
HeLa
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708205 7825521 313020840 245.94 FLP-IN_R514G_FUS-RNAi_1.fastq.gz, SRR1708205, SRR1708205.sralite SRA
SRX807462 SRP051107 OTHER SINGLE
GSM1563910: FLP-IN_R514G_FUS-RNAi_2; Homo sapiens; OTHER
Sample: SRS789065
BioProject: PRJNA270070
BioSample: SAMN03262667
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: HeLa
cell type: human cervical cancer patient imortalized
strategy: RASL-seq
cell line: HeLa
sample type: R514G + FUS-RNAi
Original files (1)
HeLa
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708206 6315581 252623240 194.62 FLP-IN_R514G_FUS-RNAi_2.fastq.gz, SRR1708206, SRR1708206.sralite SRA
SRX807463 SRP051107 OTHER SINGLE
GSM1563911: FLP-IN_R514G_FUS-RNAi_3; Homo sapiens; OTHER
Sample: SRS789067
BioProject: PRJNA270070
BioSample: SAMN03262687
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: HeLa
cell type: human cervical cancer patient imortalized
strategy: RASL-seq
cell line: HeLa
sample type: R514G + FUS-RNAi
Original files (1)
HeLa
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708207 5481364 219254560 170.79 FLP-IN_R514G_FUS-RNAi_3.fastq.gz, SRR1708207, SRR1708207.sralite SRA
SRX807464 SRP051107 OTHER SINGLE
GSM1563912: FLP-IN_R521G_FUS-RNAi_1; Homo sapiens; OTHER
Sample: SRS789068
BioProject: PRJNA270070
BioSample: SAMN03262668
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: HeLa
cell type: human cervical cancer patient imortalized
strategy: RASL-seq
cell line: HeLa
sample type: R521G + FUS-RNAi
Original files (1)
HeLa
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708208 4644313 185772520 145.18 FLP-IN_R521G_FUS-RNAi_1.fastq.gz, SRR1708208, SRR1708208.sralite SRA
SRX807465 SRP051107 OTHER SINGLE
GSM1563913: FLP-IN_R521G_FUS-RNAi_2; Homo sapiens; OTHER
Sample: SRS789069
BioProject: PRJNA270070
BioSample: SAMN03262681
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: HeLa
cell type: human cervical cancer patient imortalized
strategy: RASL-seq
cell line: HeLa
sample type: R521G + FUS-RNAi
Original files (1)
HeLa
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708209 6429566 257182640 201.28 FLP-IN_R521G_FUS-RNAi_2.fastq.gz, SRR1708209, SRR1708209.sralite SRA
SRX807466 SRP051107 OTHER SINGLE
GSM1563914: FLP-IN_R521G_FUS-RNAi_3; Homo sapiens; OTHER
Sample: SRS789075
BioProject: PRJNA270070
BioSample: SAMN03262675
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: HeLa
cell type: human cervical cancer patient imortalized
strategy: RASL-seq
cell line: HeLa
sample type: R521G + FUS-RNAi
Original files (1)
HeLa
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708210 5234438 209377520 164.72 FLP-IN_R521G_FUS-RNAi_3.fastq.gz, SRR1708210, SRR1708210.sralite SRA
SRX807467 SRP051107 OTHER SINGLE
GSM1563915: FLP-IN_P525L_FUS-RNAi_1; Homo sapiens; OTHER
Sample: SRS789070
BioProject: PRJNA270070
BioSample: SAMN03262686
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: HeLa
cell type: human cervical cancer patient imortalized
strategy: RASL-seq
cell line: HeLa
sample type: P525L + FUS-RNAi
Original files (1)
HeLa
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708211 5968525 238741000 186.2 FLP-IN_P525L_FUS-RNAi_1.fastq.gz, SRR1708211, SRR1708211.sralite SRA
SRX807468 SRP051107 OTHER SINGLE
GSM1563916: FLP-IN_P525L_FUS-RNAi_2; Homo sapiens; OTHER
Sample: SRS789071
BioProject: PRJNA270070
BioSample: SAMN03262670
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: HeLa
cell type: human cervical cancer patient imortalized
strategy: RASL-seq
cell line: HeLa
sample type: P525L + FUS-RNAi
Original files (1)
HeLa
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708212 5555913 222236520 175.74 FLP-IN_P525L_FUS-RNAi_2.fastq.gz, SRR1708212, SRR1708212.sralite SRA
SRX807469 SRP051107 OTHER SINGLE
GSM1563917: FLP-IN_P525L_FUS-RNAi_3; Homo sapiens; OTHER
Sample: SRS789072
BioProject: PRJNA270070
BioSample: SAMN03262697
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: HeLa
cell type: human cervical cancer patient imortalized
strategy: RASL-seq
cell line: HeLa
sample type: P525L + FUS-RNAi
Original files (1)
HeLa
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708213 7781717 311268680 243.42 FLP-IN_P525L_FUS-RNAi_3.fastq.gz, SRR1708213, SRR1708213.sralite SRA
SRX807470 SRP051107 OTHER SINGLE
GSM1563918: Fibroblasts_Ctrl-2_1; Homo sapiens; OTHER
Sample: SRS789073
BioProject: PRJNA270070
BioSample: SAMN03262678
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: Primary skin fibroblast
cell type: Skin biopsy from healthy control
strategy: RASL-seq
cell line: HeLa
sample type: control human fibroblasts, Kin1ALS17
Original files (1)
Primary skin fibroblast
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708214 4452240 178089600 137.51 Fibroblasts_Ctrl-2_1.fastq.gz, SRR1708214, SRR1708214.sralite SRA
SRX807471 SRP051107 OTHER SINGLE
GSM1563919: Fibroblasts_Ctrl-2_2; Homo sapiens; OTHER
Sample: SRS789074
BioProject: PRJNA270070
BioSample: SAMN03262679
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: Primary skin fibroblast
cell type: Skin biopsy from healthy control
strategy: RASL-seq
cell line: HeLa
sample type: control human fibroblasts, Kin1ALS17
Original files (1)
Primary skin fibroblast
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708215 5631202 225248080 174.64 Fibroblasts_Ctrl-2_2.fastq.gz, SRR1708215, SRR1708215.sralite SRA
SRX807472 SRP051107 OTHER SINGLE
GSM1563920: Fibroblasts_Ctrl-3_1; Homo sapiens; OTHER
Sample: SRS789076
BioProject: PRJNA270070
BioSample: SAMN03262693
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: Primary skin fibroblast
cell type: Skin biopsy from healthy control
strategy: RASL-seq
cell line: HeLa
sample type: control human fibroblasts, Kin2ALS6
Original files (1)
Primary skin fibroblast
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708216 7863252 314530080 247.15 Fibroblasts_Ctrl-3_1.fastq.gz, SRR1708216, SRR1708216.sralite SRA
SRX807473 SRP051107 OTHER SINGLE
GSM1563921: Fibroblasts_Ctrl-3_2; Homo sapiens; OTHER
Sample: SRS789077
BioProject: PRJNA270070
BioSample: SAMN03262701
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: Primary skin fibroblast
cell type: Skin biopsy from healthy control
strategy: RASL-seq
cell line: HeLa
sample type: control human fibroblasts, Kin2ALS6
Original files (1)
Primary skin fibroblast
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708217 7461047 298441880 234.61 Fibroblasts_Ctrl-3_2.fastq.gz, SRR1708217, SRR1708217.sralite SRA
SRX807474 SRP051107 OTHER SINGLE
GSM1563922: Fibroblasts_Ctrl-1_1; Homo sapiens; OTHER
Sample: SRS789078
BioProject: PRJNA270070
BioSample: SAMN03262677
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: Primary skin fibroblast
cell type: Skin biopsy from healthy control
strategy: RASL-seq
cell line: HeLa
sample type: control human fibroblasts, con1
Original files (1)
Primary skin fibroblast
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708218 3403341 136133640 107.27 Fibroblasts_Ctrl-1_1.fastq.gz, SRR1708218, SRR1708218.sralite SRA
SRX807475 SRP051107 OTHER SINGLE
GSM1563923: Fibroblasts_Ctrl-1_2; Homo sapiens; OTHER
Sample: SRS789080
BioProject: PRJNA270070
BioSample: SAMN03262666
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: Primary skin fibroblast
cell type: Skin biopsy from healthy control
strategy: RASL-seq
cell line: HeLa
sample type: control human fibroblasts, con1
Original files (1)
Primary skin fibroblast
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708219 4110612 164424480 128.94 Fibroblasts_Ctrl-1_2.fastq.gz, SRR1708219, SRR1708219.sralite SRA
SRX807476 SRP051107 OTHER SINGLE
GSM1563924: Fibroblast_R521G_1; Homo sapiens; OTHER
Sample: SRS789079
BioProject: PRJNA270070
BioSample: SAMN03262680
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: Primary skin fibroblast
cell type: Skin biopsy from ALS patient
strategy: RASL-seq
cell line: HeLa
sample type: patient fibroblasts with R521G mutation
Original files (1)
Primary skin fibroblast
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708220 4638955 185558200 145.37 Fibroblast_R521G_1.fastq.gz, SRR1708220, SRR1708220.sralite SRA
SRX807477 SRP051107 OTHER SINGLE
GSM1563925: Fibroblast_R521G_2; Homo sapiens; OTHER
Sample: SRS789081
BioProject: PRJNA270070
BioSample: SAMN03262698
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: Primary skin fibroblast
cell type: Skin biopsy from ALS patient
strategy: RASL-seq
cell line: HeLa
sample type: patient fibroblasts with R521G mutation
Original files (1)
Primary skin fibroblast
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708221 4619693 184787720 143.87 Fibroblast_R521G_2.fastq.gz, SRR1708221, SRR1708221.sralite SRA
SRX807478 SRP051107 OTHER SINGLE
GSM1563926: Fibroblast_H517Q_1; Homo sapiens; OTHER
Sample: SRS789082
BioProject: PRJNA270070
BioSample: SAMN03262704
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: Primary skin fibroblast
cell type: Skin biopsy from ALS patient
strategy: RASL-seq
cell line: HeLa
sample type: patient fibroblasts with H517Q mutation
Original files (1)
Primary skin fibroblast
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708222 1872442 74897680 57.59 Fibroblast_H517Q_1.fastq.gz, SRR1708222, SRR1708222.sralite SRA
SRX807479 SRP051107 OTHER SINGLE
GSM1563927: Fibroblast_H517Q_2; Homo sapiens; OTHER
Sample: SRS789083
BioProject: PRJNA270070
BioSample: SAMN03262660
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: Primary skin fibroblast
cell type: Skin biopsy from ALS patient
strategy: RASL-seq
cell line: HeLa
sample type: patient fibroblasts with H517Q mutation
Original files (1)
Primary skin fibroblast
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708223 2241918 89676720 69.46 Fibroblast_H517Q_2.fastq.gz, SRR1708223, SRR1708223.sralite SRA
SRX807480 SRP051107 OTHER SINGLE
GSM1563928: Fibroblast_M511nfs_1; Homo sapiens; OTHER
Sample: SRS789084
BioProject: PRJNA270070
BioSample: SAMN03262658
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: Primary skin fibroblast
cell type: Skin biopsy from ALS patient
strategy: RASL-seq
cell line: HeLa
sample type: patient fibroblasts with M511nfs*6 mutation
Original files (1)
Primary skin fibroblast
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708224 5497041 219881640 172.12 Fibroblast_M511nfs_1.fastq.gz, SRR1708224, SRR1708224.sralite SRA
SRX807481 SRP051107 OTHER SINGLE
GSM1563929: Fibroblast_M511nfs_2; Homo sapiens; OTHER
Sample: SRS789085
BioProject: PRJNA270070
BioSample: SAMN03262657
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: Primary skin fibroblast
cell type: Skin biopsy from ALS patient
strategy: RASL-seq
cell line: HeLa
sample type: patient fibroblasts with M511nfs*6 mutation
Original files (1)
Primary skin fibroblast
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708225 5090620 203624800 159.48 Fibroblast_M511nfs_2.fastq.gz, SRR1708225, SRR1708225.sralite SRA
SRX807482 SRP051107 OTHER SINGLE
GSM1563930: Fibroblasts_Ctrl-2_notreat_1; Homo sapiens; OTHER
Sample: SRS789086
BioProject: PRJNA270070
BioSample: SAMN03262659
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: Primary skin fibroblast
cell type: Skin biopsy from healthy control
strategy: RASL-seq
cell line: HeLa
sample type: control human fibroblasts, no treatment
Original files (1)
Primary skin fibroblast
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708226 5922894 236915760 184.82 Fibroblasts_Ctrl-2_notreat_1.fastq.gz, SRR1708226, SRR1708226.sralite SRA
SRX807483 SRP051107 OTHER SINGLE
GSM1563931: Fibroblasts_Ctrl-2_notreat_2; Homo sapiens; OTHER
Sample: SRS789087
BioProject: PRJNA270070
BioSample: SAMN03262661
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: Primary skin fibroblast
cell type: Skin biopsy from healthy control
strategy: RASL-seq
cell line: HeLa
sample type: control human fibroblasts, no treatment
Original files (1)
Primary skin fibroblast
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708227 4124667 164986680 129.09 Fibroblasts_Ctrl-2_notreat_2.fastq.gz, SRR1708227, SRR1708227.sralite SRA
SRX807484 SRP051107 OTHER SINGLE
GSM1563932: Fibroblasts_Ctrl-2_sham-siRNA_1; Homo sapiens; OTHER
Sample: SRS789088
BioProject: PRJNA270070
BioSample: SAMN03262662
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: Primary skin fibroblast
cell type: Skin biopsy from healthy control
strategy: RASL-seq
cell line: HeLa
sample type: control human fibroblasts, treated with sham siRNA
Original files (1)
Primary skin fibroblast
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708228 5918904 236756160 186.0 Fibroblasts_Ctrl-2_sham-siRNA_1.fastq.gz, SRR1708228, SRR1708228.sral… SRA
SRX807485 SRP051107 OTHER SINGLE
GSM1563933: Fibroblasts_Ctrl-2_sham-siRNA_2; Homo sapiens; OTHER
Sample: SRS789090
BioProject: PRJNA270070
BioSample: SAMN03262702
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: Primary skin fibroblast
cell type: Skin biopsy from healthy control
strategy: RASL-seq
cell line: HeLa
sample type: control human fibroblasts, treated with sham siRNA
Original files (1)
Primary skin fibroblast
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708229 3861672 154466880 121.22 Fibroblasts_Ctrl-2_sham-siRNA_2.fastq.gz, SRR1708229, SRR1708229.sral… SRA
SRX807486 SRP051107 OTHER SINGLE
GSM1563934: Fibroblasts_Ctrl-2_FUS-siRNA_1; Homo sapiens; OTHER
Sample: SRS789089
BioProject: PRJNA270070
BioSample: SAMN03262671
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: Primary skin fibroblast
cell type: Skin biopsy from healthy control
strategy: RASL-seq
cell line: HeLa
sample type: control human fibroblasts, treated with FUS siRNA
Original files (1)
Primary skin fibroblast
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708230 6148687 245947480 194.88 Fibroblasts_Ctrl-2_FUS-siRNA_1.fastq.gz, SRR1708230, SRR1708230.srali… SRA
SRX807487 SRP051107 OTHER SINGLE
GSM1563935: Fibroblasts_Ctrl-2_FUS-siRNA_2; Homo sapiens; OTHER
Sample: SRS789091
BioProject: PRJNA270070
BioSample: SAMN03262689
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: Primary skin fibroblast
cell type: Skin biopsy from healthy control
strategy: RASL-seq
cell line: HeLa
sample type: control human fibroblasts, treated with FUS siRNA
Original files (1)
Primary skin fibroblast
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708231 8160062 326402480 256.23 Fibroblasts_Ctrl-2_FUS-siRNA_2.fastq.gz, SRR1708231, SRR1708231.srali… SRA
SRX807488 SRP051107 OTHER SINGLE
GSM1563936: Fibroblasts_Ctrl-2_SMN-siRNA_1; Homo sapiens; OTHER
Sample: SRS789092
BioProject: PRJNA270070
BioSample: SAMN03262699
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: Primary skin fibroblast
cell type: Skin biopsy from healthy control
strategy: RASL-seq
cell line: HeLa
sample type: control human fibroblasts, treated with SMN siRNA
Original files (1)
Primary skin fibroblast
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708232 2806392 112255680 87.61 Fibroblasts_Ctrl-2_SMN-siRNA_1.fastq.gz, SRR1708232, SRR1708232.srali… SRA
SRX807489 SRP051107 OTHER SINGLE
GSM1563937: Fibroblasts_Ctrl-2_SMN-siRNA_2; Homo sapiens; OTHER
Sample: SRS789094
BioProject: PRJNA270070
BioSample: SAMN03262674
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: Primary skin fibroblast
cell type: Skin biopsy from healthy control
strategy: RASL-seq
cell line: HeLa
sample type: control human fibroblasts, treated with SMN siRNA
Original files (1)
Primary skin fibroblast
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708233 2859794 114391760 89.19 Fibroblasts_Ctrl-2_SMN-siRNA_2.fastq.gz, SRR1708233, SRR1708233.srali… SRA
SRX807490 SRP051107 OTHER SINGLE
GSM1563938: Fibroblasts_Ctrl-1_notreat_1; Homo sapiens; OTHER
Sample: SRS789093
BioProject: PRJNA270070
BioSample: SAMN03262690
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: Primary skin fibroblast
cell type: Skin biopsy from healthy control
strategy: RASL-seq
cell line: HeLa
sample type: control human fibroblasts, no treatment
Original files (1)
Primary skin fibroblast
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708234 4834255 193370200 151.21 Fibroblasts_Ctrl-1_notreat_1.fastq.gz, SRR1708234, SRR1708234.sralite SRA
SRX807491 SRP051107 OTHER SINGLE
GSM1563939: Fibroblasts_Ctrl-1_notreat_2; Homo sapiens; OTHER
Sample: SRS789095
BioProject: PRJNA270070
BioSample: SAMN03262700
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: Primary skin fibroblast
cell type: Skin biopsy from healthy control
strategy: RASL-seq
cell line: HeLa
sample type: control human fibroblasts, no treatment
Original files (1)
Primary skin fibroblast
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708235 4298700 171948000 135.75 Fibroblasts_Ctrl-1_notreat_2.fastq.gz, SRR1708235, SRR1708235.sralite SRA
SRX807492 SRP051107 OTHER SINGLE
GSM1563940: Fibroblasts_Ctrl-1_sham-siRNA_1; Homo sapiens; OTHER
Sample: SRS789096
BioProject: PRJNA270070
BioSample: SAMN03262663
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: Primary skin fibroblast
cell type: Skin biopsy from healthy control
strategy: RASL-seq
cell line: HeLa
sample type: control human fibroblasts, treated with sham siRNA
Original files (1)
Primary skin fibroblast
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708236 2823367 112934680 88.32 Fibroblasts_Ctrl-1_sham-siRNA_1.fastq.gz, SRR1708236, SRR1708236.sral… SRA
SRX807493 SRP051107 OTHER SINGLE
GSM1563941: Fibroblasts_Ctrl-1_sham-siRNA_2; Homo sapiens; OTHER
Sample: SRS789097
BioProject: PRJNA270070
BioSample: SAMN03262669
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: Primary skin fibroblast
cell type: Skin biopsy from healthy control
strategy: RASL-seq
cell line: HeLa
sample type: control human fibroblasts, treated with sham siRNA
Original files (1)
Primary skin fibroblast
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708237 2826705 113068200 88.77 Fibroblasts_Ctrl-1_sham-siRNA_2.fastq.gz, SRR1708237, SRR1708237.sral… SRA
SRX807494 SRP051107 OTHER SINGLE
GSM1563942: Fibroblasts_Ctrl-1_FUS-siRNA_1; Homo sapiens; OTHER
Sample: SRS789098
BioProject: PRJNA270070
BioSample: SAMN03262665
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: Primary skin fibroblast
cell type: Skin biopsy from healthy control
strategy: RASL-seq
cell line: HeLa
sample type: control human fibroblasts, treated with FUS siRNA
Original files (1)
Primary skin fibroblast
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708238 3684868 147394720 116.33 Fibroblasts_Ctrl-1_FUS-siRNA_1.fastq.gz, SRR1708238, SRR1708238.srali… SRA
SRX807495 SRP051107 OTHER SINGLE
GSM1563943: Fibroblasts_Ctrl-1_FUS-siRNA_2; Homo sapiens; OTHER
Sample: SRS789099
BioProject: PRJNA270070
BioSample: SAMN03262685
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: Primary skin fibroblast
cell type: Skin biopsy from healthy control
strategy: RASL-seq
cell line: HeLa
sample type: control human fibroblasts, treated with FUS siRNA
Original files (1)
Primary skin fibroblast
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708239 5050308 202012320 159.11 Fibroblasts_Ctrl-1_FUS-siRNA_2.fastq.gz, SRR1708239, SRR1708239.srali… SRA
SRX807496 SRP051107 OTHER SINGLE
GSM1563944: Fibroblasts_Ctrl-1_SMN-siRNA_1; Homo sapiens; OTHER
Sample: SRS789100
BioProject: PRJNA270070
BioSample: SAMN03262684
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: Primary skin fibroblast
cell type: Skin biopsy from healthy control
strategy: RASL-seq
cell line: HeLa
sample type: control human fibroblasts, treated with SMN siRNA
Original files (1)
Primary skin fibroblast
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708240 5579023 223160920 176.21 Fibroblasts_Ctrl-1_SMN-siRNA_1.fastq.gz, SRR1708240, SRR1708240.srali… SRA
SRX807497 SRP051107 OTHER SINGLE
GSM1563945: Fibroblasts_Ctrl-1_SMN-siRNA_2; Homo sapiens; OTHER
Sample: SRS789101
BioProject: PRJNA270070
BioSample: SAMN03262682
Platform: ILLUMINA
Instrument: Illumina HiSeq 2000
Organism: Homo sapiens
Sample attributes
source_name: Primary skin fibroblast
cell type: Skin biopsy from healthy control
strategy: RASL-seq
cell line: HeLa
sample type: control human fibroblasts, treated with SMN siRNA
Original files (1)
Primary skin fibroblast
Runs (1)
Run Spots Bases Size (MB) Files Link
SRR1708241 5503062 220122480 172.51 Fibroblasts_Ctrl-1_SMN-siRNA_2.fastq.gz, SRR1708241, SRR1708241.srali… SRA

Linked Publications (1)

ALS-causative mutations in FUS/TLS confer gain and loss of function by altered association with SMN and U1-snRNP.
Nature communications · 2015

Data Files (70)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
— Fibroblast_H517Q_1.fastq.gz OTHER 57.6 MB link
— Fibroblast_H517Q_2.fastq.gz OTHER 69.5 MB link
— Fibroblast_M511nfs_1.fastq.gz OTHER 172.1 MB link
— Fibroblast_M511nfs_2.fastq.gz OTHER 159.5 MB link
— Fibroblast_R521G_1.fastq.gz OTHER 145.4 MB link
— Fibroblast_R521G_2.fastq.gz OTHER 143.9 MB link
— Fibroblasts_Ctrl-1_1.fastq.gz OTHER 107.3 MB link
— Fibroblasts_Ctrl-1_2.fastq.gz OTHER 128.9 MB link
— Fibroblasts_Ctrl-1_FUS-siRNA_1.fastq.gz OTHER 116.3 MB link
— Fibroblasts_Ctrl-1_FUS-siRNA_2.fastq.gz OTHER 159.1 MB link
— Fibroblasts_Ctrl-1_notreat_1.fastq.gz OTHER 151.2 MB link
— Fibroblasts_Ctrl-1_notreat_2.fastq.gz OTHER 135.8 MB link
— Fibroblasts_Ctrl-1_sham-siRNA_1.fastq.gz OTHER 88.3 MB link
— Fibroblasts_Ctrl-1_sham-siRNA_1.fastq.gz OTHER 88.3 MB link
— Fibroblasts_Ctrl-1_sham-siRNA_2.fastq.gz OTHER 88.8 MB link
— Fibroblasts_Ctrl-1_SMN-siRNA_1.fastq.gz OTHER 176.2 MB link
— Fibroblasts_Ctrl-1_SMN-siRNA_2.fastq.gz OTHER 172.5 MB link
— Fibroblasts_Ctrl-2_1.fastq.gz OTHER 137.5 MB link
— Fibroblasts_Ctrl-2_2.fastq.gz OTHER 174.6 MB link
— Fibroblasts_Ctrl-2_FUS-siRNA_1.fastq.gz OTHER 194.9 MB link
— Fibroblasts_Ctrl-2_FUS-siRNA_2.fastq.gz OTHER 256.2 MB link
— Fibroblasts_Ctrl-2_notreat_1.fastq.gz OTHER 184.8 MB link
— Fibroblasts_Ctrl-2_notreat_2.fastq.gz OTHER 129.1 MB link
— Fibroblasts_Ctrl-2_sham-siRNA_1.fastq.gz OTHER 186.0 MB link
— Fibroblasts_Ctrl-2_sham-siRNA_2.fastq.gz OTHER 121.2 MB link
— Fibroblasts_Ctrl-2_SMN-siRNA_1.fastq.gz OTHER 87.6 MB link
— Fibroblasts_Ctrl-2_SMN-siRNA_2.fastq.gz OTHER 89.2 MB link
— Fibroblasts_Ctrl-3_1.fastq.gz OTHER 247.1 MB link
— Fibroblasts_Ctrl-3_2.fastq.gz OTHER 234.6 MB link
— FLP-IN_FUS-RNAi_1.fastq.gz OTHER 135.7 MB link
— FLP-IN_FUS-RNAi_1.fastq.gz OTHER 135.7 MB link
— FLP-IN_FUS-RNAi_2.fastq.gz OTHER 137.2 MB link
— FLP-IN_FUS-RNAi_3.fastq.gz OTHER 122.5 MB link
— FLP-IN_P525L_FUS-RNAi_1.fastq.gz OTHER 186.2 MB link
— FLP-IN_P525L_FUS-RNAi_2.fastq.gz OTHER 175.7 MB link
— FLP-IN_P525L_FUS-RNAi_3.fastq.gz OTHER 243.4 MB link
— FLP-IN_parental_1.fastq.gz OTHER 151.6 MB link
— FLP-IN_parental_2.fastq.gz OTHER 173.5 MB link
— FLP-IN_parental_3.fastq.gz OTHER 195.4 MB link
— FLP-IN_R495X_FUS-RNAi_1.fastq.gz OTHER 242.7 MB link
— FLP-IN_R495X_FUS-RNAi_2.fastq.gz OTHER 186.6 MB link
— FLP-IN_R495X_FUS-RNAi_3.fastq.gz OTHER 213.4 MB link
— FLP-IN_R514G_FUS-RNAi_1.fastq.gz OTHER 245.9 MB link
— FLP-IN_R514G_FUS-RNAi_2.fastq.gz OTHER 194.6 MB link
— FLP-IN_R514G_FUS-RNAi_3.fastq.gz OTHER 170.8 MB link
— FLP-IN_R521G_FUS-RNAi_1.fastq.gz OTHER 145.2 MB link
— FLP-IN_R521G_FUS-RNAi_2.fastq.gz OTHER 201.3 MB link
— FLP-IN_R521G_FUS-RNAi_3.fastq.gz OTHER 164.7 MB link
— FLP-IN_WT-FUS_FUS-RNAi_1.fastq.gz OTHER 175.6 MB link
— FLP-IN_WT-FUS_FUS-RNAi_2.fastq.gz OTHER 59.4 MB link
— FLP-IN_WT-FUS_FUS-RNAi_3.fastq.gz OTHER 162.3 MB link
— SRR1708193 OTHER 151.6 MB link
— SRR1708194 OTHER 173.5 MB link
— SRR1708195 OTHER 195.4 MB link
— SRR1708197 OTHER 137.2 MB link
— SRR1708199 OTHER 175.6 MB link
— SRR1708200 OTHER 59.4 MB link
— SRR1708201 OTHER 162.3 MB link
— SRR1708202 OTHER 242.7 MB link
— SRR1708206 OTHER 194.6 MB link
— SRR1708207 OTHER 170.8 MB link
— SRR1708209 OTHER 201.3 MB link
— SRR1708210 OTHER 164.7 MB link
— SRR1708214 OTHER 137.5 MB link
— SRR1708219 OTHER 128.9 MB link
— SRR1708221 OTHER 143.9 MB link
— SRR1708223 OTHER 69.5 MB link
— SRR1708228 OTHER 186.0 MB link
— SRR1708231 OTHER 256.2 MB link
— SRR1708233 OTHER 89.2 MB link