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ENCSR437GYG

ENCSR ENCODE
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RNA-seq on HepG2 cells treated with a CRISPR gRNA against AGGF1. (AGGF1-BGHcLV05-B)

Organism: Homo sapiens
Experiment Types:
CRISPR RNA-seq
Status: released
Contact: Brenton Graveley, UConn

Summary

CRISPR RNA-seq of AGGF1 in Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens AGGF1

Overall Design

RNA-seq on HepG2 cells treated with a CRISPR gRNA against AGGF1. (AGGF1-BGHcLV05-B)

Analysis page is available for ENCSR437GYG.

Dataset Citations (1)

A large-scale binding and functional map of human RNA-binding proteins.
PMID 32728246 · 2020 · Nature
Eric L Van Nostrand, Peter Freese, Gabriel A Pratt, Xiaofeng Wang, Xintao Wei, Rui Xiao, Steven M Blue, Jia-Yu Chen, Neal A L Cody, Daniel Dominguez, Sara Olson, Balaji Sundararaman, Lijun Zhan, Cassandra Bazile, Louis Philip Benoit Bouvrette, Julie Bergalet, Michael O Duff, Keri E Garcia, Chelsea Gelboin-Burkhart, Myles Hochman, Nicole J Lambert, Hairi Li, Michael P McGurk, Thai B Nguyen, Tsultrim Palden, Ines Rabano, Shashank Sathe, Rebecca Stanton, Amanda Su, Ruth Wang, Brian A Yee, Bing Zhou, Ashley L Louie, Stefan Aigner, Xiang-Dong Fu, Eric Lécuyer, Christopher B Burge, Brenton R Graveley, Gene W Yeo

ENCODE Metadata

Total file size: 66.5 GB
Assay: CRISPR RNA-seq
Target: AGGF1
Biosample: Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens AGGF1
Files: 92
Unique ENC file IDs: 92
File Formats
bigWig (48) tsv (19) bam (18) fastq (4) tar (3)
Output types (11)
alignments (12) minus strand signal of all reads (12) minus strand signal of unique reads (12) plus strand signal of all reads (12) plus strand signal of unique reads (12) transcript quantifications (8) gene quantifications (6) transcriptome alignments (6) differential expression quantifications (5) reads (4) differential splicing quantifications (3)

Linked Publications (1)

Data Files (92)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
ENCFF011BLH ENCFF011BLH.bam bam (alignments); assembly: GRCh38; genome annotation: V24 alignments GRCh38 2.8 GB link
ENCFF081ZZA ENCFF081ZZA.bam bam (alignments); assembly: GRCh38; genome annotation: V24 alignments GRCh38 1.8 GB link
ENCFF205VOJ ENCFF205VOJ.bam bam (alignments); assembly: GRCh38; genome annotation: V24 alignments GRCh38 2.2 GB link
ENCFF754QFX ENCFF754QFX.bam bam (alignments); assembly: GRCh38; genome annotation: V24 alignments GRCh38 3.5 GB link
ENCFF245GPX ENCFF245GPX.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 2.2 GB link
ENCFF935DEE ENCFF935DEE.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 3.5 GB link
ENCFF209KPC ENCFF209KPC.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 2.8 GB link
ENCFF248IJX ENCFF248IJX.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 2.2 GB link
ENCFF265NOS ENCFF265NOS.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 1.8 GB link
ENCFF586UMA ENCFF586UMA.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 1.8 GB link
ENCFF644TFQ ENCFF644TFQ.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 2.8 GB link
ENCFF658NAS ENCFF658NAS.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 3.4 GB link
ENCFF317YVP ENCFF317YVP.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V24 transcriptome alignments GRCh38 3.1 GB link
ENCFF740PMW ENCFF740PMW.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V24 transcriptome alignments GRCh38 5.0 GB link
ENCFF372TUQ ENCFF372TUQ.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 3.6 GB link
ENCFF742VAE ENCFF742VAE.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 5.8 GB link
ENCFF556NLI ENCFF556NLI.bam bam (transcriptome alignments); assembly: hg19; genome annotation: V19 transcriptome alignments hg19 3.1 GB link
ENCFF785HQU ENCFF785HQU.bam bam (transcriptome alignments); assembly: hg19; genome annotation: V19 transcriptome alignments hg19 5.0 GB link
ENCFF111KCP ENCFF111KCP.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V24 minus strand signal of all reads GRCh38 67.2 MB link
ENCFF398SOA ENCFF398SOA.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V24 minus strand signal of all reads GRCh38 74.8 MB link
ENCFF551UNB ENCFF551UNB.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V24 minus strand signal of all reads GRCh38 55.5 MB link
ENCFF668XNU ENCFF668XNU.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V24 minus strand signal of all reads GRCh38 50.0 MB link
ENCFF150XKI ENCFF150XKI.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 55.5 MB link
ENCFF533OWZ ENCFF533OWZ.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 74.8 MB link
ENCFF046DBY ENCFF046DBY.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 65.1 MB link
ENCFF125CRX ENCFF125CRX.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 55.6 MB link
ENCFF214EBA ENCFF214EBA.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 48.2 MB link
ENCFF610VTX ENCFF610VTX.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 73.6 MB link
ENCFF810YEV ENCFF810YEV.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 68.4 MB link
ENCFF975NYE ENCFF975NYE.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 51.1 MB link
ENCFF373XJR ENCFF373XJR.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 minus strand signal of unique reads GRCh38 49.4 MB link
ENCFF591VSF ENCFF591VSF.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 minus strand signal of unique reads GRCh38 66.2 MB link
ENCFF799OTO ENCFF799OTO.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 minus strand signal of unique reads GRCh38 62.6 MB link
ENCFF905JAE ENCFF905JAE.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 minus strand signal of unique reads GRCh38 46.9 MB link
ENCFF222VUK ENCFF222VUK.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 66.0 MB link
ENCFF575LVR ENCFF575LVR.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 49.3 MB link
ENCFF102EPI ENCFF102EPI.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 47.6 MB link
ENCFF164UBR ENCFF164UBR.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 60.1 MB link
ENCFF337FOE ENCFF337FOE.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 63.3 MB link
ENCFF665RPB ENCFF665RPB.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 44.7 MB link
ENCFF666YCS ENCFF666YCS.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 66.6 MB link
ENCFF713DGU ENCFF713DGU.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 49.9 MB link
ENCFF100FDF ENCFF100FDF.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V24 plus strand signal of all reads GRCh38 68.0 MB link
ENCFF259LLW ENCFF259LLW.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V24 plus strand signal of all reads GRCh38 50.5 MB link
ENCFF494IUW ENCFF494IUW.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V24 plus strand signal of all reads GRCh38 75.7 MB link
ENCFF695YZL ENCFF695YZL.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V24 plus strand signal of all reads GRCh38 56.1 MB link
ENCFF687ZJO ENCFF687ZJO.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 75.7 MB link
ENCFF799MFH ENCFF799MFH.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 56.1 MB link
ENCFF068EFW ENCFF068EFW.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 48.7 MB link
ENCFF208URS ENCFF208URS.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 51.7 MB link
ENCFF321ZAQ ENCFF321ZAQ.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 56.1 MB link
ENCFF332OWC ENCFF332OWC.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 74.4 MB link
ENCFF960GVO ENCFF960GVO.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 65.9 MB link
ENCFF971XJN ENCFF971XJN.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 69.3 MB link
ENCFF243NXR ENCFF243NXR.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 plus strand signal of unique reads GRCh38 63.8 MB link
ENCFF340TGV ENCFF340TGV.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 plus strand signal of unique reads GRCh38 50.4 MB link
ENCFF409NGZ ENCFF409NGZ.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 plus strand signal of unique reads GRCh38 47.8 MB link
ENCFF558UTY ENCFF558UTY.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 plus strand signal of unique reads GRCh38 67.6 MB link
ENCFF773GBZ ENCFF773GBZ.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 50.3 MB link
ENCFF983GZL ENCFF983GZL.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 67.4 MB link
ENCFF255IXQ ENCFF255IXQ.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 68.1 MB link
ENCFF255XOC ENCFF255XOC.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 48.6 MB link
ENCFF267IVA ENCFF267IVA.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 64.7 MB link
ENCFF463AUE ENCFF463AUE.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 45.5 MB link
ENCFF521KJQ ENCFF521KJQ.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 51.0 MB link
ENCFF579INI ENCFF579INI.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 61.3 MB link
ENCFF226VRJ ENCFF226VRJ.fastq fastq (reads) reads 2.2 GB link
ENCFF732QPC ENCFF732QPC.fastq fastq (reads) reads 1.3 GB link
ENCFF828KDR ENCFF828KDR.fastq fastq (reads) reads 2.1 GB link
ENCFF887NBC ENCFF887NBC.fastq fastq (reads) reads 1.4 GB link
ENCFF253QSU ENCFF253QSU.tar tar (differential splicing quantifications); assembly: hg19; genome annotation: V19 differential splicing quantifications hg19 2.9 MB link
ENCFF568NUK ENCFF568NUK.tar tar (differential splicing quantifications); assembly: hg19; genome annotation: V19 differential splicing quantifications hg19 12.8 MB link
ENCFF950XMT ENCFF950XMT.tar tar (differential splicing quantifications); assembly: hg19; genome annotation: V19 differential splicing quantifications hg19 3.4 MB link
ENCFF110CEB ENCFF110CEB.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 1.8 MB link
ENCFF174YMP ENCFF174YMP.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 2.8 MB link
ENCFF527GHH ENCFF527GHH.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 5.6 MB link
ENCFF779HYK ENCFF779HYK.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 1.9 MB link
ENCFF935TNW ENCFF935TNW.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 4.1 MB link
ENCFF168CVX ENCFF168CVX.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V24 gene quantifications GRCh38 9.5 MB link
ENCFF989USQ ENCFF989USQ.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V24 gene quantifications GRCh38 9.4 MB link
ENCFF291KGL ENCFF291KGL.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF423RQM ENCFF423RQM.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF057UVW ENCFF057UVW.tsv tsv (gene quantifications); assembly: hg19; genome annotation: V19 gene quantifications hg19 9.1 MB link
ENCFF819IWF ENCFF819IWF.tsv tsv (gene quantifications); assembly: hg19; genome annotation: V19 gene quantifications hg19 9.1 MB link
ENCFF143BMK ENCFF143BMK.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V24 transcript quantifications GRCh38 25.4 MB link
ENCFF411EAH ENCFF411EAH.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V24 transcript quantifications GRCh38 25.3 MB link
ENCFF013DPY ENCFF013DPY.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.2 MB link
ENCFF356FQT ENCFF356FQT.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.9 MB link
ENCFF703ZYK ENCFF703ZYK.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.8 MB link
ENCFF998NHD ENCFF998NHD.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.3 MB link
ENCFF443RQD ENCFF443RQD.tsv tsv (transcript quantifications); assembly: hg19; genome annotation: V19 transcript quantifications hg19 25.0 MB link
ENCFF497LRO ENCFF497LRO.tsv tsv (transcript quantifications); assembly: hg19; genome annotation: V19 transcript quantifications hg19 24.9 MB link