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ENCSR406SEH

ENCSR ENCODE
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RNA-seq on K562 cells treated with a CRISPR gRNA against EXOSC5. (EXOSC5-BGKcLV06)

Organism: Homo sapiens
Experiment Types:
CRISPR RNA-seq
Status: released
Contact: Brenton Graveley, UConn

Summary

CRISPR RNA-seq of EXOSC5 in Homo sapiens K562 genetically modified (deletion) using CRISPR targeting H. sapiens EXOSC5

Overall Design

RNA-seq on K562 cells treated with a CRISPR gRNA against EXOSC5. (EXOSC5-BGKcLV06)

Analysis page is available for ENCSR406SEH.

Dataset Citations (1)

A large-scale binding and functional map of human RNA-binding proteins.
PMID 32728246 · 2020 · Nature
Eric L Van Nostrand, Peter Freese, Gabriel A Pratt, Xiaofeng Wang, Xintao Wei, Rui Xiao, Steven M Blue, Jia-Yu Chen, Neal A L Cody, Daniel Dominguez, Sara Olson, Balaji Sundararaman, Lijun Zhan, Cassandra Bazile, Louis Philip Benoit Bouvrette, Julie Bergalet, Michael O Duff, Keri E Garcia, Chelsea Gelboin-Burkhart, Myles Hochman, Nicole J Lambert, Hairi Li, Michael P McGurk, Thai B Nguyen, Tsultrim Palden, Ines Rabano, Shashank Sathe, Rebecca Stanton, Amanda Su, Ruth Wang, Brian A Yee, Bing Zhou, Ashley L Louie, Stefan Aigner, Xiang-Dong Fu, Eric Lécuyer, Christopher B Burge, Brenton R Graveley, Gene W Yeo

ENCODE Metadata

Total file size: 82.4 GB
Assay: CRISPR RNA-seq
Target: EXOSC5
Biosample: Homo sapiens K562 genetically modified (deletion) using CRISPR targeting H. sapiens EXOSC5
Files: 92
Unique ENC file IDs: 92
File Formats
bigWig (48) tsv (19) bam (18) fastq (4) tar (3)
Output types (11)
alignments (12) minus strand signal of all reads (12) minus strand signal of unique reads (12) plus strand signal of all reads (12) plus strand signal of unique reads (12) transcript quantifications (8) gene quantifications (6) transcriptome alignments (6) differential expression quantifications (5) reads (4) differential splicing quantifications (3)

Linked Publications (1)

Data Files (92)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
ENCFF016HOA ENCFF016HOA.bam bam (alignments) alignments 3.2 GB link
ENCFF342XFY ENCFF342XFY.bam bam (alignments) alignments 3.4 GB link
ENCFF351JWB ENCFF351JWB.bam bam (alignments) alignments 4.0 GB link
ENCFF352IAS ENCFF352IAS.bam bam (alignments) alignments 3.5 GB link
ENCFF607FTT ENCFF607FTT.bam bam (alignments) alignments 2.8 GB link
ENCFF644DDX ENCFF644DDX.bam bam (alignments) alignments 2.8 GB link
ENCFF749JGK ENCFF749JGK.bam bam (alignments) alignments 4.3 GB link
ENCFF756WPD ENCFF756WPD.bam bam (alignments) alignments 4.3 GB link
ENCFF795BLZ ENCFF795BLZ.bam bam (alignments) alignments 2.8 GB link
ENCFF826WCC ENCFF826WCC.bam bam (alignments) alignments 3.2 GB link
ENCFF869ACK ENCFF869ACK.bam bam (alignments) alignments 3.5 GB link
ENCFF911NQE ENCFF911NQE.bam bam (alignments) alignments 3.4 GB link
ENCFF101DOO ENCFF101DOO.bam bam (transcriptome alignments) transcriptome alignments 4.2 GB link
ENCFF207AOM ENCFF207AOM.bam bam (transcriptome alignments) transcriptome alignments 4.2 GB link
ENCFF426AYS ENCFF426AYS.bam bam (transcriptome alignments) transcriptome alignments 5.6 GB link
ENCFF521TIY ENCFF521TIY.bam bam (transcriptome alignments) transcriptome alignments 4.8 GB link
ENCFF777VFR ENCFF777VFR.bam bam (transcriptome alignments) transcriptome alignments 4.9 GB link
ENCFF851VKJ ENCFF851VKJ.bam bam (transcriptome alignments) transcriptome alignments 4.8 GB link
ENCFF006HBC ENCFF006HBC.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 79.9 MB link
ENCFF054NOE ENCFF054NOE.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 88.5 MB link
ENCFF076GET ENCFF076GET.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 78.6 MB link
ENCFF090VWQ ENCFF090VWQ.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 82.6 MB link
ENCFF244LZU ENCFF244LZU.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 92.5 MB link
ENCFF256BDT ENCFF256BDT.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 88.6 MB link
ENCFF604VPG ENCFF604VPG.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 75.3 MB link
ENCFF743WLQ ENCFF743WLQ.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 83.4 MB link
ENCFF755CWC ENCFF755CWC.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 93.9 MB link
ENCFF802BZS ENCFF802BZS.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 79.7 MB link
ENCFF883ATT ENCFF883ATT.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 86.8 MB link
ENCFF946DOM ENCFF946DOM.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 93.9 MB link
ENCFF025CBS ENCFF025CBS.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 72.6 MB link
ENCFF145SXX ENCFF145SXX.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 82.0 MB link
ENCFF159BOQ ENCFF159BOQ.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 78.0 MB link
ENCFF360DAA ENCFF360DAA.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 77.1 MB link
ENCFF362NYO ENCFF362NYO.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 73.2 MB link
ENCFF438DZM ENCFF438DZM.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 81.2 MB link
ENCFF521VSR ENCFF521VSR.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 68.5 MB link
ENCFF553BUE ENCFF553BUE.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 76.8 MB link
ENCFF646LMT ENCFF646LMT.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 76.3 MB link
ENCFF693TGT ENCFF693TGT.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 77.7 MB link
ENCFF698YCS ENCFF698YCS.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 72.3 MB link
ENCFF960OMR ENCFF960OMR.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 81.4 MB link
ENCFF033LUU ENCFF033LUU.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 89.7 MB link
ENCFF119WJZ ENCFF119WJZ.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 95.1 MB link
ENCFF140AXV ENCFF140AXV.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 76.7 MB link
ENCFF300KAS ENCFF300KAS.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 95.1 MB link
ENCFF380SIA ENCFF380SIA.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 89.7 MB link
ENCFF508IUG ENCFF508IUG.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 81.3 MB link
ENCFF516JLL ENCFF516JLL.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 79.5 MB link
ENCFF522IHV ENCFF522IHV.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 81.2 MB link
ENCFF535IUG ENCFF535IUG.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 93.7 MB link
ENCFF558JKU ENCFF558JKU.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 87.9 MB link
ENCFF605FBD ENCFF605FBD.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 83.6 MB link
ENCFF989FRE ENCFF989FRE.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 84.8 MB link
ENCFF034SXL ENCFF034SXL.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 79.7 MB link
ENCFF097NCD ENCFF097NCD.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 74.4 MB link
ENCFF099ZIJ ENCFF099ZIJ.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 78.7 MB link
ENCFF124RUI ENCFF124RUI.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 77.9 MB link
ENCFF194CRB ENCFF194CRB.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 78.8 MB link
ENCFF247KWP ENCFF247KWP.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 83.0 MB link
ENCFF395IFY ENCFF395IFY.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 75.1 MB link
ENCFF453PCG ENCFF453PCG.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 74.2 MB link
ENCFF483XLX ENCFF483XLX.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 79.6 MB link
ENCFF508LXX ENCFF508LXX.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 83.9 MB link
ENCFF551KLI ENCFF551KLI.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 83.4 MB link
ENCFF647FOH ENCFF647FOH.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 70.5 MB link
ENCFF294FVZ ENCFF294FVZ.fastq fastq (reads) reads 2.0 GB link
ENCFF395GPY ENCFF395GPY.fastq fastq (reads) reads 2.3 GB link
ENCFF531FDU ENCFF531FDU.fastq fastq (reads) reads 2.3 GB link
ENCFF754RHC ENCFF754RHC.fastq fastq (reads) reads 2.0 GB link
ENCFF369YBC ENCFF369YBC.tar tar (differential splicing quantifications) differential splicing quantifications 3.6 MB link
ENCFF738THL ENCFF738THL.tar tar (differential splicing quantifications) differential splicing quantifications 4.3 MB link
ENCFF867JUI ENCFF867JUI.tar tar (differential splicing quantifications) differential splicing quantifications 14.9 MB link
ENCFF115HVD ENCFF115HVD.tsv tsv (differential expression quantifications) differential expression quantifications 4.3 MB link
ENCFF175GIP ENCFF175GIP.tsv tsv (differential expression quantifications) differential expression quantifications 5.6 MB link
ENCFF745BEC ENCFF745BEC.tsv tsv (differential expression quantifications) differential expression quantifications 1.7 MB link
ENCFF804FMP ENCFF804FMP.tsv tsv (differential expression quantifications) differential expression quantifications 2.0 MB link
ENCFF886HZN ENCFF886HZN.tsv tsv (differential expression quantifications) differential expression quantifications 2.9 MB link
ENCFF004QBG ENCFF004QBG.tsv tsv (gene quantifications) gene quantifications 9.1 MB link
ENCFF182JUI ENCFF182JUI.tsv tsv (gene quantifications) gene quantifications 9.5 MB link
ENCFF283SUC ENCFF283SUC.tsv tsv (gene quantifications) gene quantifications 10.4 MB link
ENCFF309DAF ENCFF309DAF.tsv tsv (gene quantifications) gene quantifications 10.4 MB link
ENCFF695LGF ENCFF695LGF.tsv tsv (gene quantifications) gene quantifications 9.1 MB link
ENCFF770KDN ENCFF770KDN.tsv tsv (gene quantifications) gene quantifications 9.4 MB link
ENCFF092KWC ENCFF092KWC.tsv tsv (transcript quantifications) transcript quantifications 27.4 MB link
ENCFF121NWA ENCFF121NWA.tsv tsv (transcript quantifications) transcript quantifications 27.4 MB link
ENCFF161CSN ENCFF161CSN.tsv tsv (transcript quantifications) transcript quantifications 29.8 MB link
ENCFF257VMU ENCFF257VMU.tsv tsv (transcript quantifications) transcript quantifications 25.3 MB link
ENCFF391FHL ENCFF391FHL.tsv tsv (transcript quantifications) transcript quantifications 24.9 MB link
ENCFF491YEG ENCFF491YEG.tsv tsv (transcript quantifications) transcript quantifications 24.9 MB link
ENCFF887NIM ENCFF887NIM.tsv tsv (transcript quantifications) transcript quantifications 25.3 MB link
ENCFF894FJA ENCFF894FJA.tsv tsv (transcript quantifications) transcript quantifications 29.8 MB link