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ENCSR861ENA

ENCSR ENCODE
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shRNA knockdown against EIF2S1 in HepG2 cells followed by RNA-seq. (EIF2S1-BGHLV12)

Organism: Homo sapiens
Experiment Types:
shRNA RNA-seq
Status: released
Contact: Brenton Graveley, UConn

Summary

shRNA RNA-seq of EIF2S1 in Homo sapiens HepG2 expressing RNAi targeting H. sapiens EIF2S1

Overall Design

shRNA knockdown against EIF2S1 in HepG2 cells followed by RNA-seq. (EIF2S1-BGHLV12)

Analysis page is available for ENCSR861ENA.

Dataset Citations (3)

A large-scale binding and functional map of human RNA-binding proteins.
PMID 32728246 · 2020 · Nature
Eric L Van Nostrand, Peter Freese, Gabriel A Pratt, Xiaofeng Wang, Xintao Wei, Rui Xiao, Steven M Blue, Jia-Yu Chen, Neal A L Cody, Daniel Dominguez, Sara Olson, Balaji Sundararaman, Lijun Zhan, Cassandra Bazile, Louis Philip Benoit Bouvrette, Julie Bergalet, Michael O Duff, Keri E Garcia, Chelsea Gelboin-Burkhart, Myles Hochman, Nicole J Lambert, Hairi Li, Michael P McGurk, Thai B Nguyen, Tsultrim Palden, Ines Rabano, Shashank Sathe, Rebecca Stanton, Amanda Su, Ruth Wang, Brian A Yee, Bing Zhou, Ashley L Louie, Stefan Aigner, Xiang-Dong Fu, Eric Lécuyer, Christopher B Burge, Brenton R Graveley, Gene W Yeo

ENCODE Metadata

Total file size: 125.8 GB
Assay: shRNA RNA-seq
Target: EIF2S1
Biosample: Homo sapiens HepG2 expressing RNAi targeting H. sapiens EIF2S1
Files: 92
Unique ENC file IDs: 92
File Formats
bigWig (48) tsv (19) bam (18) fastq (4) tar (3)
Output types (11)
alignments (12) minus strand signal of all reads (12) minus strand signal of unique reads (12) plus strand signal of all reads (12) plus strand signal of unique reads (12) transcript quantifications (8) gene quantifications (6) transcriptome alignments (6) differential expression quantifications (5) reads (4) differential splicing quantifications (3)

Linked Publications (1)

Data Files (92)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
ENCFF064LCJ ENCFF064LCJ.bam bam (alignments) alignments 5.2 GB link
ENCFF188MUD ENCFF188MUD.bam bam (alignments) alignments 5.0 GB link
ENCFF287DXE ENCFF287DXE.bam bam (alignments) alignments 4.5 GB link
ENCFF432ATJ ENCFF432ATJ.bam bam (alignments) alignments 4.7 GB link
ENCFF458PTC ENCFF458PTC.bam bam (alignments) alignments 6.1 GB link
ENCFF560TAA ENCFF560TAA.bam bam (alignments) alignments 5.1 GB link
ENCFF610AAZ ENCFF610AAZ.bam bam (alignments) alignments 6.1 GB link
ENCFF699NBD ENCFF699NBD.bam bam (alignments) alignments 4.5 GB link
ENCFF776MXG ENCFF776MXG.bam bam (alignments) alignments 5.9 GB link
ENCFF783KCK ENCFF783KCK.bam bam (alignments) alignments 5.0 GB link
ENCFF857MUO ENCFF857MUO.bam bam (alignments) alignments 5.7 GB link
ENCFF879ZAE ENCFF879ZAE.bam bam (alignments) alignments 5.7 GB link
ENCFF139ROY ENCFF139ROY.bam bam (transcriptome alignments) transcriptome alignments 8.7 GB link
ENCFF226ZHP ENCFF226ZHP.bam bam (transcriptome alignments) transcriptome alignments 7.7 GB link
ENCFF427KIB ENCFF427KIB.bam bam (transcriptome alignments) transcriptome alignments 6.9 GB link
ENCFF543HFN ENCFF543HFN.bam bam (transcriptome alignments) transcriptome alignments 7.8 GB link
ENCFF605JAX ENCFF605JAX.bam bam (transcriptome alignments) transcriptome alignments 6.9 GB link
ENCFF793CAZ ENCFF793CAZ.bam bam (transcriptome alignments) transcriptome alignments 7.8 GB link
ENCFF005IVF ENCFF005IVF.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 92.7 MB link
ENCFF231LOC ENCFF231LOC.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 88.1 MB link
ENCFF380FSP ENCFF380FSP.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 81.5 MB link
ENCFF480TDO ENCFF480TDO.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 79.8 MB link
ENCFF482AIY ENCFF482AIY.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 92.7 MB link
ENCFF577XRQ ENCFF577XRQ.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 88.2 MB link
ENCFF587DYJ ENCFF587DYJ.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 83.1 MB link
ENCFF697YTT ENCFF697YTT.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 81.9 MB link
ENCFF732ERS ENCFF732ERS.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 87.5 MB link
ENCFF757BLK ENCFF757BLK.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 77.9 MB link
ENCFF766XCL ENCFF766XCL.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 92.7 MB link
ENCFF893BTS ENCFF893BTS.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 84.5 MB link
ENCFF203GDJ ENCFF203GDJ.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 71.9 MB link
ENCFF240LOQ ENCFF240LOQ.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 78.9 MB link
ENCFF312DCI ENCFF312DCI.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 79.0 MB link
ENCFF356VXI ENCFF356VXI.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 75.2 MB link
ENCFF489WCJ ENCFF489WCJ.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 82.7 MB link
ENCFF704QFI ENCFF704QFI.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 83.1 MB link
ENCFF715SYX ENCFF715SYX.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 83.6 MB link
ENCFF862JDA ENCFF862JDA.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 74.9 MB link
ENCFF951AFL ENCFF951AFL.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 79.1 MB link
ENCFF961CWD ENCFF961CWD.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 78.9 MB link
ENCFF965DFG ENCFF965DFG.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 76.5 MB link
ENCFF987OKI ENCFF987OKI.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 79.2 MB link
ENCFF008SXV ENCFF008SXV.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 82.7 MB link
ENCFF032ZUM ENCFF032ZUM.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 89.3 MB link
ENCFF127TFO ENCFF127TFO.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 93.9 MB link
ENCFF506IYU ENCFF506IYU.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 93.9 MB link
ENCFF782AYV ENCFF782AYV.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 79.0 MB link
ENCFF787YVM ENCFF787YVM.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 93.9 MB link
ENCFF792HKL ENCFF792HKL.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 89.3 MB link
ENCFF816LFC ENCFF816LFC.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 85.9 MB link
ENCFF824UOL ENCFF824UOL.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 88.5 MB link
ENCFF830SGK ENCFF830SGK.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 84.2 MB link
ENCFF881JJI ENCFF881JJI.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 80.9 MB link
ENCFF928OQA ENCFF928OQA.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 83.2 MB link
ENCFF127XPM ENCFF127XPM.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 76.8 MB link
ENCFF137WXI ENCFF137WXI.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 84.7 MB link
ENCFF179KQA ENCFF179KQA.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 81.0 MB link
ENCFF387IPN ENCFF387IPN.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 76.7 MB link
ENCFF392GWF ENCFF392GWF.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 80.8 MB link
ENCFF416DPO ENCFF416DPO.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 73.7 MB link
ENCFF420DKY ENCFF420DKY.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 78.6 MB link
ENCFF423MPN ENCFF423MPN.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 80.8 MB link
ENCFF484BQB ENCFF484BQB.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 80.5 MB link
ENCFF537TAR ENCFF537TAR.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 81.1 MB link
ENCFF749JVP ENCFF749JVP.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 85.0 MB link
ENCFF967UGD ENCFF967UGD.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 85.6 MB link
ENCFF245HYS ENCFF245HYS.fastq fastq (reads) reads 3.0 GB link
ENCFF392XBJ ENCFF392XBJ.fastq fastq (reads) reads 3.3 GB link
ENCFF405UJA ENCFF405UJA.fastq fastq (reads) reads 2.9 GB link
ENCFF711IKC ENCFF711IKC.fastq fastq (reads) reads 3.3 GB link
ENCFF130GUN ENCFF130GUN.tar tar (differential splicing quantifications) differential splicing quantifications 16.4 MB link
ENCFF418NVI ENCFF418NVI.tar tar (differential splicing quantifications) differential splicing quantifications 3.1 MB link
ENCFF819TTB ENCFF819TTB.tar tar (differential splicing quantifications) differential splicing quantifications 3.8 MB link
ENCFF243GFU ENCFF243GFU.tsv tsv (differential expression quantifications) differential expression quantifications 5.6 MB link
ENCFF273VIE ENCFF273VIE.tsv tsv (differential expression quantifications) differential expression quantifications 1.9 MB link
ENCFF328RWO ENCFF328RWO.tsv tsv (differential expression quantifications) differential expression quantifications 3.0 MB link
ENCFF378RUW ENCFF378RUW.tsv tsv (differential expression quantifications) differential expression quantifications 1.7 MB link
ENCFF416VKJ ENCFF416VKJ.tsv tsv (differential expression quantifications) differential expression quantifications 4.4 MB link
ENCFF037QEG ENCFF037QEG.tsv tsv (gene quantifications) gene quantifications 9.1 MB link
ENCFF161VPA ENCFF161VPA.tsv tsv (gene quantifications) gene quantifications 9.1 MB link
ENCFF179DQK ENCFF179DQK.tsv tsv (gene quantifications) gene quantifications 9.4 MB link
ENCFF329RNX ENCFF329RNX.tsv tsv (gene quantifications) gene quantifications 10.4 MB link
ENCFF789GUB ENCFF789GUB.tsv tsv (gene quantifications) gene quantifications 10.4 MB link
ENCFF944GVM ENCFF944GVM.tsv tsv (gene quantifications) gene quantifications 9.5 MB link
ENCFF132JUG ENCFF132JUG.tsv tsv (transcript quantifications) transcript quantifications 27.4 MB link
ENCFF286BLM ENCFF286BLM.tsv tsv (transcript quantifications) transcript quantifications 25.3 MB link
ENCFF472MPN ENCFF472MPN.tsv tsv (transcript quantifications) transcript quantifications 27.3 MB link
ENCFF549TRW ENCFF549TRW.tsv tsv (transcript quantifications) transcript quantifications 24.9 MB link
ENCFF573ZTC ENCFF573ZTC.tsv tsv (transcript quantifications) transcript quantifications 25.3 MB link
ENCFF694QWV ENCFF694QWV.tsv tsv (transcript quantifications) transcript quantifications 24.9 MB link
ENCFF820MPR ENCFF820MPR.tsv tsv (transcript quantifications) transcript quantifications 29.8 MB link
ENCFF997ECP ENCFF997ECP.tsv tsv (transcript quantifications) transcript quantifications 29.8 MB link