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ENCSR181RLB

ENCSR ENCODE
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shRNA knockdown against EEF2 in K562 cells followed by RNA-seq. (EEF2_BGKLV19)

Organism: Homo sapiens
Experiment Types:
shRNA RNA-seq
Status: released
Contact: Brenton Graveley, UConn

Summary

shRNA RNA-seq of EEF2 in Homo sapiens K562 expressing RNAi targeting H. sapiens EEF2

Overall Design

shRNA knockdown against EEF2 in K562 cells followed by RNA-seq. (EEF2_BGKLV19)

Analysis page is available for ENCSR181RLB.

Dataset Citations (3)

A large-scale binding and functional map of human RNA-binding proteins.
PMID 32728246 · 2020 · Nature
Eric L Van Nostrand, Peter Freese, Gabriel A Pratt, Xiaofeng Wang, Xintao Wei, Rui Xiao, Steven M Blue, Jia-Yu Chen, Neal A L Cody, Daniel Dominguez, Sara Olson, Balaji Sundararaman, Lijun Zhan, Cassandra Bazile, Louis Philip Benoit Bouvrette, Julie Bergalet, Michael O Duff, Keri E Garcia, Chelsea Gelboin-Burkhart, Myles Hochman, Nicole J Lambert, Hairi Li, Michael P McGurk, Thai B Nguyen, Tsultrim Palden, Ines Rabano, Shashank Sathe, Rebecca Stanton, Amanda Su, Ruth Wang, Brian A Yee, Bing Zhou, Ashley L Louie, Stefan Aigner, Xiang-Dong Fu, Eric Lécuyer, Christopher B Burge, Brenton R Graveley, Gene W Yeo

ENCODE Metadata

Total file size: 97.6 GB
Assay: shRNA RNA-seq
Target: EEF2
Biosample: Homo sapiens K562 expressing RNAi targeting H. sapiens EEF2
Files: 92
Unique ENC file IDs: 92
File Formats
bigWig (48) tsv (19) bam (18) fastq (4) tar (3)
Output types (11)
alignments (12) minus strand signal of all reads (12) minus strand signal of unique reads (12) plus strand signal of all reads (12) plus strand signal of unique reads (12) transcript quantifications (8) gene quantifications (6) transcriptome alignments (6) differential expression quantifications (5) reads (4) differential splicing quantifications (3)

Linked Publications (1)

Data Files (92)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
ENCFF603UEH ENCFF603UEH.bam bam (alignments); assembly: GRCh38; genome annotation: V24 alignments GRCh38 3.0 GB link
ENCFF613QDI ENCFF613QDI.bam bam (alignments); assembly: GRCh38; genome annotation: V24 alignments GRCh38 3.6 GB link
ENCFF693HAJ ENCFF693HAJ.bam bam (alignments); assembly: GRCh38; genome annotation: V24 alignments GRCh38 4.6 GB link
ENCFF978PEN ENCFF978PEN.bam bam (alignments); assembly: GRCh38; genome annotation: V24 alignments GRCh38 5.6 GB link
ENCFF291DKJ ENCFF291DKJ.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 5.6 GB link
ENCFF802NDY ENCFF802NDY.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 3.6 GB link
ENCFF066MXD ENCFF066MXD.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 4.5 GB link
ENCFF333ZWZ ENCFF333ZWZ.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 4.6 GB link
ENCFF507NQE ENCFF507NQE.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 3.0 GB link
ENCFF805TZL ENCFF805TZL.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 5.4 GB link
ENCFF887SBE ENCFF887SBE.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 3.5 GB link
ENCFF951AQX ENCFF951AQX.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 3.0 GB link
ENCFF369OQI ENCFF369OQI.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V24 transcriptome alignments GRCh38 4.3 GB link
ENCFF618PRH ENCFF618PRH.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V24 transcriptome alignments GRCh38 6.6 GB link
ENCFF410TOT ENCFF410TOT.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 4.8 GB link
ENCFF992CGB ENCFF992CGB.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 7.5 GB link
ENCFF651QFZ ENCFF651QFZ.bam bam (transcriptome alignments); assembly: hg19; genome annotation: V19 transcriptome alignments hg19 6.6 GB link
ENCFF804JHU ENCFF804JHU.bam bam (transcriptome alignments); assembly: hg19; genome annotation: V19 transcriptome alignments hg19 4.2 GB link
ENCFF084TIH ENCFF084TIH.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V24 minus strand signal of all reads GRCh38 77.0 MB link
ENCFF462RGF ENCFF462RGF.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V24 minus strand signal of all reads GRCh38 87.3 MB link
ENCFF626ANJ ENCFF626ANJ.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V24 minus strand signal of all reads GRCh38 69.0 MB link
ENCFF656MDJ ENCFF656MDJ.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V24 minus strand signal of all reads GRCh38 96.9 MB link
ENCFF222DQD ENCFF222DQD.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 77.0 MB link
ENCFF560GBY ENCFF560GBY.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 97.0 MB link
ENCFF415TRD ENCFF415TRD.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 95.9 MB link
ENCFF535MID ENCFF535MID.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 88.4 MB link
ENCFF645EJH ENCFF645EJH.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 86.4 MB link
ENCFF722EPU ENCFF722EPU.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 75.6 MB link
ENCFF902QMU ENCFF902QMU.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 70.1 MB link
ENCFF961XEF ENCFF961XEF.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 66.9 MB link
ENCFF115HST ENCFF115HST.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 minus strand signal of unique reads GRCh38 81.5 MB link
ENCFF124YEN ENCFF124YEN.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 minus strand signal of unique reads GRCh38 85.7 MB link
ENCFF295SVZ ENCFF295SVZ.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 minus strand signal of unique reads GRCh38 67.7 MB link
ENCFF990OUX ENCFF990OUX.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 minus strand signal of unique reads GRCh38 64.0 MB link
ENCFF779QPQ ENCFF779QPQ.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 86.2 MB link
ENCFF888JVS ENCFF888JVS.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 67.8 MB link
ENCFF048IBN ENCFF048IBN.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 66.8 MB link
ENCFF268RHL ENCFF268RHL.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 82.0 MB link
ENCFF522SNH ENCFF522SNH.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 61.1 MB link
ENCFF637GTO ENCFF637GTO.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 85.2 MB link
ENCFF744TOL ENCFF744TOL.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 79.4 MB link
ENCFF830GUR ENCFF830GUR.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 64.4 MB link
ENCFF065OJQ ENCFF065OJQ.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V24 plus strand signal of all reads GRCh38 98.0 MB link
ENCFF494MDP ENCFF494MDP.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V24 plus strand signal of all reads GRCh38 69.8 MB link
ENCFF697QOY ENCFF697QOY.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V24 plus strand signal of all reads GRCh38 77.9 MB link
ENCFF962WNH ENCFF962WNH.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V24 plus strand signal of all reads GRCh38 88.2 MB link
ENCFF496YVY ENCFF496YVY.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 98.0 MB link
ENCFF862HNT ENCFF862HNT.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 77.9 MB link
ENCFF155WAR ENCFF155WAR.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 96.9 MB link
ENCFF364JCD ENCFF364JCD.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 87.8 MB link
ENCFF446PSY ENCFF446PSY.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 68.2 MB link
ENCFF821TTY ENCFF821TTY.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 89.7 MB link
ENCFF843UMF ENCFF843UMF.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 76.4 MB link
ENCFF979PMY ENCFF979PMY.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 71.3 MB link
ENCFF091RRA ENCFF091RRA.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 plus strand signal of unique reads GRCh38 69.2 MB link
ENCFF532AXC ENCFF532AXC.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 plus strand signal of unique reads GRCh38 65.3 MB link
ENCFF768OTT ENCFF768OTT.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 plus strand signal of unique reads GRCh38 83.1 MB link
ENCFF825MOJ ENCFF825MOJ.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 plus strand signal of unique reads GRCh38 87.5 MB link
ENCFF563HCH ENCFF563HCH.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 69.3 MB link
ENCFF651NJY ENCFF651NJY.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 88.0 MB link
ENCFF060QPW ENCFF060QPW.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 81.5 MB link
ENCFF568ELF ENCFF568ELF.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 87.2 MB link
ENCFF632LMC ENCFF632LMC.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 68.4 MB link
ENCFF796OER ENCFF796OER.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 66.0 MB link
ENCFF845LWB ENCFF845LWB.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 84.0 MB link
ENCFF914GJX ENCFF914GJX.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 62.8 MB link
ENCFF647QUX ENCFF647QUX.fastq fastq (reads) reads 2.9 GB link
ENCFF763GZO ENCFF763GZO.fastq fastq (reads) reads 3.0 GB link
ENCFF808PNY ENCFF808PNY.fastq fastq (reads) reads 1.9 GB link
ENCFF923JKP ENCFF923JKP.fastq fastq (reads) reads 1.8 GB link
ENCFF157ZEB ENCFF157ZEB.tar tar (differential splicing quantifications); assembly: hg19; genome annotation: V19 differential splicing quantifications hg19 14.8 MB link
ENCFF396TVT ENCFF396TVT.tar tar (differential splicing quantifications); assembly: hg19; genome annotation: V19 differential splicing quantifications hg19 3.6 MB link
ENCFF726OHY ENCFF726OHY.tar tar (differential splicing quantifications); assembly: hg19; genome annotation: V19 differential splicing quantifications hg19 4.1 MB link
ENCFF230UEX ENCFF230UEX.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 2.9 MB link
ENCFF264WTQ ENCFF264WTQ.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 5.5 MB link
ENCFF533ULV ENCFF533ULV.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 1.9 MB link
ENCFF598VNI ENCFF598VNI.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 4.2 MB link
ENCFF849AIT ENCFF849AIT.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 1.7 MB link
ENCFF073ODL ENCFF073ODL.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V24 gene quantifications GRCh38 9.4 MB link
ENCFF347FZA ENCFF347FZA.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V24 gene quantifications GRCh38 9.5 MB link
ENCFF547PGQ ENCFF547PGQ.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF642VSI ENCFF642VSI.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF185XHP ENCFF185XHP.tsv tsv (gene quantifications); assembly: hg19; genome annotation: V19 gene quantifications hg19 9.1 MB link
ENCFF444LQA ENCFF444LQA.tsv tsv (gene quantifications); assembly: hg19; genome annotation: V19 gene quantifications hg19 9.1 MB link
ENCFF673TWY ENCFF673TWY.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V24 transcript quantifications GRCh38 25.3 MB link
ENCFF947VQL ENCFF947VQL.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V24 transcript quantifications GRCh38 25.3 MB link
ENCFF156YNJ ENCFF156YNJ.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.8 MB link
ENCFF591HRW ENCFF591HRW.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.8 MB link
ENCFF903YUA ENCFF903YUA.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.4 MB link
ENCFF964LWI ENCFF964LWI.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.3 MB link
ENCFF345NVT ENCFF345NVT.tsv tsv (transcript quantifications); assembly: hg19; genome annotation: V19 transcript quantifications hg19 24.9 MB link
ENCFF722UMS ENCFF722UMS.tsv tsv (transcript quantifications); assembly: hg19; genome annotation: V19 transcript quantifications hg19 24.9 MB link