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ENCSR584LDM

ENCSR ENCODE
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shRNA knockdown against DDX27 in K562 cells followed by RNA-seq. (DDX27_BGKLV19)

Organism: Homo sapiens
Experiment Types:
shRNA RNA-seq
Status: released
Contact: Brenton Graveley, UConn

Summary

shRNA RNA-seq of DDX27 in Homo sapiens K562 expressing RNAi targeting H. sapiens DDX27

Overall Design

shRNA knockdown against DDX27 in K562 cells followed by RNA-seq. (DDX27_BGKLV19)

Analysis page is available for ENCSR584LDM.

Dataset Citations (3)

A large-scale binding and functional map of human RNA-binding proteins.
PMID 32728246 · 2020 · Nature
Eric L Van Nostrand, Peter Freese, Gabriel A Pratt, Xiaofeng Wang, Xintao Wei, Rui Xiao, Steven M Blue, Jia-Yu Chen, Neal A L Cody, Daniel Dominguez, Sara Olson, Balaji Sundararaman, Lijun Zhan, Cassandra Bazile, Louis Philip Benoit Bouvrette, Julie Bergalet, Michael O Duff, Keri E Garcia, Chelsea Gelboin-Burkhart, Myles Hochman, Nicole J Lambert, Hairi Li, Michael P McGurk, Thai B Nguyen, Tsultrim Palden, Ines Rabano, Shashank Sathe, Rebecca Stanton, Amanda Su, Ruth Wang, Brian A Yee, Bing Zhou, Ashley L Louie, Stefan Aigner, Xiang-Dong Fu, Eric Lécuyer, Christopher B Burge, Brenton R Graveley, Gene W Yeo

ENCODE Metadata

Total file size: 72.2 GB
Assay: shRNA RNA-seq
Target: DDX27
Biosample: Homo sapiens K562 expressing RNAi targeting H. sapiens DDX27
Files: 92
Unique ENC file IDs: 92
File Formats
bigWig (48) tsv (19) bam (18) fastq (4) tar (3)
Output types (11)
alignments (12) minus strand signal of all reads (12) minus strand signal of unique reads (12) plus strand signal of all reads (12) plus strand signal of unique reads (12) transcript quantifications (8) gene quantifications (6) transcriptome alignments (6) differential expression quantifications (5) reads (4) differential splicing quantifications (3)

Linked Publications (1)

Data Files (92)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
ENCFF350HHZ ENCFF350HHZ.bam bam (alignments); assembly: GRCh38; genome annotation: V24 alignments GRCh38 2.8 GB link
ENCFF521ODI ENCFF521ODI.bam bam (alignments); assembly: GRCh38; genome annotation: V24 alignments GRCh38 2.8 GB link
ENCFF606AVE ENCFF606AVE.bam bam (alignments); assembly: GRCh38; genome annotation: V24 alignments GRCh38 3.4 GB link
ENCFF956DKX ENCFF956DKX.bam bam (alignments); assembly: GRCh38; genome annotation: V24 alignments GRCh38 3.4 GB link
ENCFF338TJG ENCFF338TJG.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 3.4 GB link
ENCFF379TMX ENCFF379TMX.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 3.4 GB link
ENCFF141XOO ENCFF141XOO.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 2.8 GB link
ENCFF146GMR ENCFF146GMR.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 2.8 GB link
ENCFF342HXE ENCFF342HXE.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 2.8 GB link
ENCFF580AHO ENCFF580AHO.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 3.3 GB link
ENCFF634FHL ENCFF634FHL.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 3.3 GB link
ENCFF829OWA ENCFF829OWA.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 2.8 GB link
ENCFF681LTQ ENCFF681LTQ.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V24 transcriptome alignments GRCh38 4.0 GB link
ENCFF743QOZ ENCFF743QOZ.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V24 transcriptome alignments GRCh38 4.0 GB link
ENCFF320COY ENCFF320COY.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 4.5 GB link
ENCFF907HLJ ENCFF907HLJ.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 4.5 GB link
ENCFF094WXB ENCFF094WXB.bam bam (transcriptome alignments); assembly: hg19; genome annotation: V19 transcriptome alignments hg19 4.0 GB link
ENCFF438VFN ENCFF438VFN.bam bam (transcriptome alignments); assembly: hg19; genome annotation: V19 transcriptome alignments hg19 3.9 GB link
ENCFF197WFB ENCFF197WFB.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V24 minus strand signal of all reads GRCh38 74.4 MB link
ENCFF208FDQ ENCFF208FDQ.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V24 minus strand signal of all reads GRCh38 66.0 MB link
ENCFF546FYB ENCFF546FYB.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V24 minus strand signal of all reads GRCh38 66.6 MB link
ENCFF905YGS ENCFF905YGS.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V24 minus strand signal of all reads GRCh38 73.6 MB link
ENCFF305GPN ENCFF305GPN.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 73.6 MB link
ENCFF879FVW ENCFF879FVW.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 74.5 MB link
ENCFF082NEG ENCFF082NEG.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 63.9 MB link
ENCFF238XZL ENCFF238XZL.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 66.3 MB link
ENCFF263ACW ENCFF263ACW.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 68.0 MB link
ENCFF502JNB ENCFF502JNB.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 72.7 MB link
ENCFF591PED ENCFF591PED.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 64.4 MB link
ENCFF921OOG ENCFF921OOG.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 72.6 MB link
ENCFF207DND ENCFF207DND.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 minus strand signal of unique reads GRCh38 61.5 MB link
ENCFF246BLT ENCFF246BLT.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 minus strand signal of unique reads GRCh38 64.5 MB link
ENCFF704JXU ENCFF704JXU.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 minus strand signal of unique reads GRCh38 65.1 MB link
ENCFF905LXI ENCFF905LXI.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 minus strand signal of unique reads GRCh38 62.3 MB link
ENCFF792CNX ENCFF792CNX.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 64.9 MB link
ENCFF899ITK ENCFF899ITK.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 65.9 MB link
ENCFF344UWW ENCFF344UWW.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 62.2 MB link
ENCFF492IJK ENCFF492IJK.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 60.7 MB link
ENCFF519BIQ ENCFF519BIQ.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 58.8 MB link
ENCFF694RDM ENCFF694RDM.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 58.9 MB link
ENCFF940JRF ENCFF940JRF.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 64.3 MB link
ENCFF967VRA ENCFF967VRA.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 64.9 MB link
ENCFF056FMA ENCFF056FMA.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V24 plus strand signal of all reads GRCh38 67.6 MB link
ENCFF174NFC ENCFF174NFC.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V24 plus strand signal of all reads GRCh38 75.4 MB link
ENCFF186UCQ ENCFF186UCQ.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V24 plus strand signal of all reads GRCh38 66.8 MB link
ENCFF446BZP ENCFF446BZP.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V24 plus strand signal of all reads GRCh38 74.4 MB link
ENCFF044HUX ENCFF044HUX.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 75.5 MB link
ENCFF158KCK ENCFF158KCK.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 74.4 MB link
ENCFF140MTO ENCFF140MTO.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 69.2 MB link
ENCFF196SUI ENCFF196SUI.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 67.3 MB link
ENCFF493AEW ENCFF493AEW.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 64.9 MB link
ENCFF789BQF ENCFF789BQF.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 73.4 MB link
ENCFF921RGZ ENCFF921RGZ.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 73.6 MB link
ENCFF941XQB ENCFF941XQB.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 65.6 MB link
ENCFF380SHI ENCFF380SHI.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 plus strand signal of unique reads GRCh38 63.7 MB link
ENCFF497CDP ENCFF497CDP.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 plus strand signal of unique reads GRCh38 62.7 MB link
ENCFF680IAH ENCFF680IAH.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 plus strand signal of unique reads GRCh38 66.6 MB link
ENCFF781UBT ENCFF781UBT.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 plus strand signal of unique reads GRCh38 65.9 MB link
ENCFF156YVT ENCFF156YVT.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 66.3 MB link
ENCFF556PUO ENCFF556PUO.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 67.4 MB link
ENCFF103ZGK ENCFF103ZGK.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 60.6 MB link
ENCFF219FOX ENCFF219FOX.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 65.8 MB link
ENCFF315VFH ENCFF315VFH.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 66.6 MB link
ENCFF495BGW ENCFF495BGW.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 62.1 MB link
ENCFF657NTC ENCFF657NTC.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 63.8 MB link
ENCFF775ZKR ENCFF775ZKR.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 60.4 MB link
ENCFF008LRD ENCFF008LRD.fastq fastq (reads) reads 1.7 GB link
ENCFF423NQN ENCFF423NQN.fastq fastq (reads) reads 1.7 GB link
ENCFF648GRM ENCFF648GRM.fastq fastq (reads) reads 1.8 GB link
ENCFF992VRQ ENCFF992VRQ.fastq fastq (reads) reads 1.8 GB link
ENCFF153IOI ENCFF153IOI.tar tar (differential splicing quantifications); assembly: hg19; genome annotation: V19 differential splicing quantifications hg19 14.7 MB link
ENCFF302RND ENCFF302RND.tar tar (differential splicing quantifications); assembly: hg19; genome annotation: V19 differential splicing quantifications hg19 3.4 MB link
ENCFF551NBH ENCFF551NBH.tar tar (differential splicing quantifications); assembly: hg19; genome annotation: V19 differential splicing quantifications hg19 3.9 MB link
ENCFF040GAJ ENCFF040GAJ.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 1.7 MB link
ENCFF159QMN ENCFF159QMN.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 4.2 MB link
ENCFF319FCJ ENCFF319FCJ.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 1.9 MB link
ENCFF632VDC ENCFF632VDC.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 2.8 MB link
ENCFF653CWH ENCFF653CWH.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 5.5 MB link
ENCFF422RCG ENCFF422RCG.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V24 gene quantifications GRCh38 9.4 MB link
ENCFF589HWI ENCFF589HWI.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V24 gene quantifications GRCh38 9.4 MB link
ENCFF676GGK ENCFF676GGK.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF733MSA ENCFF733MSA.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF069EQO ENCFF069EQO.tsv tsv (gene quantifications); assembly: hg19; genome annotation: V19 gene quantifications hg19 9.1 MB link
ENCFF661NOK ENCFF661NOK.tsv tsv (gene quantifications); assembly: hg19; genome annotation: V19 gene quantifications hg19 9.1 MB link
ENCFF003VIV ENCFF003VIV.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V24 transcript quantifications GRCh38 25.3 MB link
ENCFF584MVH ENCFF584MVH.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V24 transcript quantifications GRCh38 25.3 MB link
ENCFF078IZS ENCFF078IZS.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.8 MB link
ENCFF243HAN ENCFF243HAN.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.3 MB link
ENCFF637LLY ENCFF637LLY.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.8 MB link
ENCFF761CYF ENCFF761CYF.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.3 MB link
ENCFF120ICS ENCFF120ICS.tsv tsv (transcript quantifications); assembly: hg19; genome annotation: V19 transcript quantifications hg19 24.8 MB link
ENCFF237IIP ENCFF237IIP.tsv tsv (transcript quantifications); assembly: hg19; genome annotation: V19 transcript quantifications hg19 24.8 MB link