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ENCSR296ERI

ENCSR ENCODE
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RNA-seq on HepG2 cells treated with an shRNA knockdown against PUS1. (PUS1_BGHLV31)

Organism: Homo sapiens
Experiment Types:
shRNA RNA-seq
Status: released
Contact: Brenton Graveley, UConn

Summary

shRNA RNA-seq of PUS1 in Homo sapiens HepG2 expressing RNAi targeting H. sapiens PUS1

Overall Design

RNA-seq on HepG2 cells treated with an shRNA knockdown against PUS1. (PUS1_BGHLV31)

Analysis page is available for ENCSR296ERI.

Dataset Citations (3)

A large-scale binding and functional map of human RNA-binding proteins.
PMID 32728246 · 2020 · Nature
Eric L Van Nostrand, Peter Freese, Gabriel A Pratt, Xiaofeng Wang, Xintao Wei, Rui Xiao, Steven M Blue, Jia-Yu Chen, Neal A L Cody, Daniel Dominguez, Sara Olson, Balaji Sundararaman, Lijun Zhan, Cassandra Bazile, Louis Philip Benoit Bouvrette, Julie Bergalet, Michael O Duff, Keri E Garcia, Chelsea Gelboin-Burkhart, Myles Hochman, Nicole J Lambert, Hairi Li, Michael P McGurk, Thai B Nguyen, Tsultrim Palden, Ines Rabano, Shashank Sathe, Rebecca Stanton, Amanda Su, Ruth Wang, Brian A Yee, Bing Zhou, Ashley L Louie, Stefan Aigner, Xiang-Dong Fu, Eric Lécuyer, Christopher B Burge, Brenton R Graveley, Gene W Yeo

ENCODE Metadata

Total file size: 88.2 GB
Assay: shRNA RNA-seq
Target: PUS1
Biosample: Homo sapiens HepG2 expressing RNAi targeting H. sapiens PUS1
Files: 92
Unique ENC file IDs: 92
File Formats
bigWig (48) tsv (19) bam (18) fastq (4) tar (3)
Output types (11)
alignments (12) minus strand signal of all reads (12) minus strand signal of unique reads (12) plus strand signal of all reads (12) plus strand signal of unique reads (12) transcript quantifications (8) gene quantifications (6) transcriptome alignments (6) differential expression quantifications (5) reads (4) differential splicing quantifications (3)

Linked Publications (1)

Data Files (92)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
ENCFF310CCU ENCFF310CCU.bam bam (alignments); assembly: GRCh38; genome annotation: V24 alignments GRCh38 3.0 GB link
ENCFF442UIQ ENCFF442UIQ.bam bam (alignments); assembly: GRCh38; genome annotation: V24 alignments GRCh38 3.3 GB link
ENCFF745XZP ENCFF745XZP.bam bam (alignments); assembly: GRCh38; genome annotation: V24 alignments GRCh38 4.1 GB link
ENCFF996LET ENCFF996LET.bam bam (alignments); assembly: GRCh38; genome annotation: V24 alignments GRCh38 3.8 GB link
ENCFF062LYO ENCFF062LYO.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 4.1 GB link
ENCFF554RYZ ENCFF554RYZ.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 3.8 GB link
ENCFF020ZBS ENCFF020ZBS.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 2.9 GB link
ENCFF087JNB ENCFF087JNB.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 3.2 GB link
ENCFF317YKN ENCFF317YKN.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 3.9 GB link
ENCFF377BWN ENCFF377BWN.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 2.9 GB link
ENCFF727ATR ENCFF727ATR.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 3.6 GB link
ENCFF965KMK ENCFF965KMK.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 3.2 GB link
ENCFF885GLF ENCFF885GLF.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V24 transcriptome alignments GRCh38 5.5 GB link
ENCFF890KXW ENCFF890KXW.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V24 transcriptome alignments GRCh38 5.1 GB link
ENCFF496YDE ENCFF496YDE.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 5.8 GB link
ENCFF789WTY ENCFF789WTY.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 6.3 GB link
ENCFF374UDW ENCFF374UDW.bam bam (transcriptome alignments); assembly: hg19; genome annotation: V19 transcriptome alignments hg19 5.5 GB link
ENCFF574QJN ENCFF574QJN.bam bam (transcriptome alignments); assembly: hg19; genome annotation: V19 transcriptome alignments hg19 5.0 GB link
ENCFF579YNE ENCFF579YNE.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V24 minus strand signal of all reads GRCh38 91.5 MB link
ENCFF626UFV ENCFF626UFV.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V24 minus strand signal of all reads GRCh38 95.1 MB link
ENCFF703EZT ENCFF703EZT.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V24 minus strand signal of all reads GRCh38 85.9 MB link
ENCFF917SFP ENCFF917SFP.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V24 minus strand signal of all reads GRCh38 82.9 MB link
ENCFF457BEY ENCFF457BEY.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 91.5 MB link
ENCFF896FNQ ENCFF896FNQ.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 95.1 MB link
ENCFF095PST ENCFF095PST.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 95.0 MB link
ENCFF585OVC ENCFF585OVC.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 90.8 MB link
ENCFF722OSK ENCFF722OSK.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 85.1 MB link
ENCFF753QWH ENCFF753QWH.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 81.4 MB link
ENCFF772SMN ENCFF772SMN.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 88.1 MB link
ENCFF953SLK ENCFF953SLK.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 85.3 MB link
ENCFF269FHM ENCFF269FHM.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 minus strand signal of unique reads GRCh38 77.9 MB link
ENCFF283SGE ENCFF283SGE.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 minus strand signal of unique reads GRCh38 84.8 MB link
ENCFF530MNB ENCFF530MNB.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 minus strand signal of unique reads GRCh38 82.2 MB link
ENCFF969YUD ENCFF969YUD.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 minus strand signal of unique reads GRCh38 81.0 MB link
ENCFF064QFC ENCFF064QFC.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 82.1 MB link
ENCFF647AEA ENCFF647AEA.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 85.2 MB link
ENCFF090LNW ENCFF090LNW.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 80.9 MB link
ENCFF156VCD ENCFF156VCD.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 78.2 MB link
ENCFF192QLL ENCFF192QLL.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 82.4 MB link
ENCFF244GCE ENCFF244GCE.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 85.5 MB link
ENCFF294YEQ ENCFF294YEQ.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 75.1 MB link
ENCFF771SJB ENCFF771SJB.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 78.8 MB link
ENCFF380TNU ENCFF380TNU.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V24 plus strand signal of all reads GRCh38 93.6 MB link
ENCFF899IVX ENCFF899IVX.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V24 plus strand signal of all reads GRCh38 87.7 MB link
ENCFF953STT ENCFF953STT.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V24 plus strand signal of all reads GRCh38 84.9 MB link
ENCFF968SIB ENCFF968SIB.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V24 plus strand signal of all reads GRCh38 97.1 MB link
ENCFF551VAB ENCFF551VAB.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 93.6 MB link
ENCFF703HLE ENCFF703HLE.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 97.1 MB link
ENCFF094XAH ENCFF094XAH.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 87.3 MB link
ENCFF235WDT ENCFF235WDT.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 96.8 MB link
ENCFF332UXW ENCFF332UXW.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 92.6 MB link
ENCFF430XES ENCFF430XES.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 87.5 MB link
ENCFF460RYY ENCFF460RYY.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 90.1 MB link
ENCFF857IOT ENCFF857IOT.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 83.5 MB link
ENCFF394EVN ENCFF394EVN.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 plus strand signal of unique reads GRCh38 83.3 MB link
ENCFF555VST ENCFF555VST.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 plus strand signal of unique reads GRCh38 84.7 MB link
ENCFF595RQL ENCFF595RQL.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 plus strand signal of unique reads GRCh38 80.3 MB link
ENCFF614VDC ENCFF614VDC.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 plus strand signal of unique reads GRCh38 87.3 MB link
ENCFF273NJN ENCFF273NJN.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 87.7 MB link
ENCFF316WRA ENCFF316WRA.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 84.6 MB link
ENCFF077SLF ENCFF077SLF.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 85.0 MB link
ENCFF192JKX ENCFF192JKX.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 81.5 MB link
ENCFF273DWK ENCFF273DWK.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 80.8 MB link
ENCFF380NRP ENCFF380NRP.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 83.4 MB link
ENCFF627HSF ENCFF627HSF.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 77.9 MB link
ENCFF992UAR ENCFF992UAR.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 88.1 MB link
ENCFF495SUF ENCFF495SUF.fastq fastq (reads) reads 2.1 GB link
ENCFF559ZLS ENCFF559ZLS.fastq fastq (reads) reads 2.1 GB link
ENCFF853NIO ENCFF853NIO.fastq fastq (reads) reads 2.3 GB link
ENCFF988WOT ENCFF988WOT.fastq fastq (reads) reads 2.3 GB link
ENCFF167DMX ENCFF167DMX.tar tar (differential splicing quantifications); assembly: hg19; genome annotation: V19 differential splicing quantifications hg19 3.5 MB link
ENCFF265PGK ENCFF265PGK.tar tar (differential splicing quantifications); assembly: hg19; genome annotation: V19 differential splicing quantifications hg19 17.1 MB link
ENCFF958TBN ENCFF958TBN.tar tar (differential splicing quantifications); assembly: hg19; genome annotation: V19 differential splicing quantifications hg19 4.2 MB link
ENCFF111LTX ENCFF111LTX.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 1.7 MB link
ENCFF165VFE ENCFF165VFE.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 5.5 MB link
ENCFF240GVH ENCFF240GVH.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 3.0 MB link
ENCFF316GJO ENCFF316GJO.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 2.0 MB link
ENCFF327CZK ENCFF327CZK.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 4.4 MB link
ENCFF569DZG ENCFF569DZG.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V24 gene quantifications GRCh38 9.5 MB link
ENCFF706WCI ENCFF706WCI.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V24 gene quantifications GRCh38 9.5 MB link
ENCFF320JLE ENCFF320JLE.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF406NWM ENCFF406NWM.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF569YNW ENCFF569YNW.tsv tsv (gene quantifications); assembly: hg19; genome annotation: V19 gene quantifications hg19 9.1 MB link
ENCFF743UIE ENCFF743UIE.tsv tsv (gene quantifications); assembly: hg19; genome annotation: V19 gene quantifications hg19 9.1 MB link
ENCFF023FAS ENCFF023FAS.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V24 transcript quantifications GRCh38 25.3 MB link
ENCFF420EGL ENCFF420EGL.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V24 transcript quantifications GRCh38 25.3 MB link
ENCFF066FUY ENCFF066FUY.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.8 MB link
ENCFF262TXO ENCFF262TXO.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.4 MB link
ENCFF556XKJ ENCFF556XKJ.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.8 MB link
ENCFF777KNS ENCFF777KNS.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.4 MB link
ENCFF327EDE ENCFF327EDE.tsv tsv (transcript quantifications); assembly: hg19; genome annotation: V19 transcript quantifications hg19 24.9 MB link
ENCFF407KHY ENCFF407KHY.tsv tsv (transcript quantifications); assembly: hg19; genome annotation: V19 transcript quantifications hg19 24.9 MB link