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ENCSR342EDG

ENCSR ENCODE
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shRNA knockdown against U2AF1 in K562 cells followed by RNA-seq. (U2AF1-LV08)

Organism: Homo sapiens
Experiment Types:
shRNA RNA-seq
Status: released
Contact: Brenton Graveley, UConn

Summary

shRNA RNA-seq of U2AF1 in Homo sapiens K562 expressing RNAi targeting H. sapiens U2AF1L5

Overall Design

shRNA knockdown against U2AF1 in K562 cells followed by RNA-seq. (U2AF1-LV08)

Analysis page is available for ENCSR342EDG.

Dataset Citations (3)

A large-scale binding and functional map of human RNA-binding proteins.
PMID 32728246 · 2020 · Nature
Eric L Van Nostrand, Peter Freese, Gabriel A Pratt, Xiaofeng Wang, Xintao Wei, Rui Xiao, Steven M Blue, Jia-Yu Chen, Neal A L Cody, Daniel Dominguez, Sara Olson, Balaji Sundararaman, Lijun Zhan, Cassandra Bazile, Louis Philip Benoit Bouvrette, Julie Bergalet, Michael O Duff, Keri E Garcia, Chelsea Gelboin-Burkhart, Myles Hochman, Nicole J Lambert, Hairi Li, Michael P McGurk, Thai B Nguyen, Tsultrim Palden, Ines Rabano, Shashank Sathe, Rebecca Stanton, Amanda Su, Ruth Wang, Brian A Yee, Bing Zhou, Ashley L Louie, Stefan Aigner, Xiang-Dong Fu, Eric Lécuyer, Christopher B Burge, Brenton R Graveley, Gene W Yeo

ENCODE Metadata

Total file size: 84.1 GB
Assay: shRNA RNA-seq
Target: U2AF1
Biosample: Homo sapiens K562 expressing RNAi targeting H. sapiens U2AF1L5
Files: 92
Unique ENC file IDs: 92
File Formats
bigWig (48) tsv (19) bam (18) fastq (4) tar (3)
Output types (11)
alignments (12) minus strand signal of all reads (12) minus strand signal of unique reads (12) plus strand signal of all reads (12) plus strand signal of unique reads (12) transcript quantifications (8) gene quantifications (6) transcriptome alignments (6) differential expression quantifications (5) reads (4) differential splicing quantifications (3)

Linked Publications (1)

Data Files (92)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
ENCFF033CDK ENCFF033CDK.bam bam (alignments) alignments 3.5 GB link
ENCFF049WEE ENCFF049WEE.bam bam (alignments) alignments 4.1 GB link
ENCFF141MVP ENCFF141MVP.bam bam (alignments) alignments 3.4 GB link
ENCFF323XGR ENCFF323XGR.bam bam (alignments) alignments 4.2 GB link
ENCFF352HEO ENCFF352HEO.bam bam (alignments) alignments 4.0 GB link
ENCFF426YHJ ENCFF426YHJ.bam bam (alignments) alignments 3.5 GB link
ENCFF462PAF ENCFF462PAF.bam bam (alignments) alignments 4.2 GB link
ENCFF612TNR ENCFF612TNR.bam bam (alignments) alignments 4.1 GB link
ENCFF707GKG ENCFF707GKG.bam bam (alignments) alignments 3.4 GB link
ENCFF750CBD ENCFF750CBD.bam bam (alignments) alignments 3.4 GB link
ENCFF912ONP ENCFF912ONP.bam bam (alignments) alignments 3.4 GB link
ENCFF919WVS ENCFF919WVS.bam bam (alignments) alignments 4.2 GB link
ENCFF048WLT ENCFF048WLT.bam bam (transcriptome alignments) transcriptome alignments 4.1 GB link
ENCFF121YLS ENCFF121YLS.bam bam (transcriptome alignments) transcriptome alignments 4.1 GB link
ENCFF297GEV ENCFF297GEV.bam bam (transcriptome alignments) transcriptome alignments 4.1 GB link
ENCFF483EDG ENCFF483EDG.bam bam (transcriptome alignments) transcriptome alignments 4.6 GB link
ENCFF732JRP ENCFF732JRP.bam bam (transcriptome alignments) transcriptome alignments 4.7 GB link
ENCFF992EJT ENCFF992EJT.bam bam (transcriptome alignments) transcriptome alignments 4.1 GB link
ENCFF011FSV ENCFF011FSV.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 82.7 MB link
ENCFF104BVL ENCFF104BVL.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 90.7 MB link
ENCFF143FEK ENCFF143FEK.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 82.7 MB link
ENCFF206ODQ ENCFF206ODQ.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 77.7 MB link
ENCFF234FVQ ENCFF234FVQ.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 77.7 MB link
ENCFF293WKB ENCFF293WKB.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 91.9 MB link
ENCFF303QAF ENCFF303QAF.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 93.5 MB link
ENCFF390YKN ENCFF390YKN.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 82.1 MB link
ENCFF763PVQ ENCFF763PVQ.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 92.4 MB link
ENCFF861UFQ ENCFF861UFQ.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 91.9 MB link
ENCFF955ZCR ENCFF955ZCR.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 93.5 MB link
ENCFF986NRK ENCFF986NRK.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 84.8 MB link
ENCFF010BKS ENCFF010BKS.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 80.7 MB link
ENCFF163DSN ENCFF163DSN.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 70.9 MB link
ENCFF226ZIE ENCFF226ZIE.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 81.9 MB link
ENCFF309XDW ENCFF309XDW.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 77.5 MB link
ENCFF438IZT ENCFF438IZT.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 75.6 MB link
ENCFF452JDU ENCFF452JDU.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 76.5 MB link
ENCFF490MYP ENCFF490MYP.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 70.9 MB link
ENCFF561LLM ENCFF561LLM.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 81.5 MB link
ENCFF724EPK ENCFF724EPK.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 80.9 MB link
ENCFF729CQK ENCFF729CQK.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 81.8 MB link
ENCFF880QCJ ENCFF880QCJ.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 81.0 MB link
ENCFF928WXN ENCFF928WXN.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 76.5 MB link
ENCFF230NUE ENCFF230NUE.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 79.7 MB link
ENCFF256BRG ENCFF256BRG.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 92.4 MB link
ENCFF323VJB ENCFF323VJB.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 86.8 MB link
ENCFF387SKB ENCFF387SKB.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 93.6 MB link
ENCFF629YGZ ENCFF629YGZ.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 95.1 MB link
ENCFF672ABP ENCFF672ABP.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 93.6 MB link
ENCFF678XSH ENCFF678XSH.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 83.6 MB link
ENCFF734AAX ENCFF734AAX.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 84.7 MB link
ENCFF857BEI ENCFF857BEI.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 95.1 MB link
ENCFF880EBP ENCFF880EBP.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 94.1 MB link
ENCFF914ADV ENCFF914ADV.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 84.2 MB link
ENCFF927RUL ENCFF927RUL.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 79.6 MB link
ENCFF075HTM ENCFF075HTM.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 83.3 MB link
ENCFF184ITL ENCFF184ITL.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 84.3 MB link
ENCFF269UED ENCFF269UED.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 83.3 MB link
ENCFF278ILO ENCFF278ILO.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 80.5 MB link
ENCFF373TYZ ENCFF373TYZ.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 85.1 MB link
ENCFF384ZVJ ENCFF384ZVJ.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 73.3 MB link
ENCFF437VCE ENCFF437VCE.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 85.6 MB link
ENCFF741KHZ ENCFF741KHZ.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 73.3 MB link
ENCFF803WEP ENCFF803WEP.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 78.9 MB link
ENCFF827FJK ENCFF827FJK.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 79.7 MB link
ENCFF854YPF ENCFF854YPF.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 84.4 MB link
ENCFF944FMC ENCFF944FMC.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 77.8 MB link
ENCFF049UBT ENCFF049UBT.fastq fastq (reads) reads 2.2 GB link
ENCFF365IMS ENCFF365IMS.fastq fastq (reads) reads 2.3 GB link
ENCFF670ZRN ENCFF670ZRN.fastq fastq (reads) reads 2.2 GB link
ENCFF725MSZ ENCFF725MSZ.fastq fastq (reads) reads 2.2 GB link
ENCFF215LUY ENCFF215LUY.tar tar (differential splicing quantifications) differential splicing quantifications 3.3 MB link
ENCFF404KPH ENCFF404KPH.tar tar (differential splicing quantifications) differential splicing quantifications 4.1 MB link
ENCFF728YIQ ENCFF728YIQ.tar tar (differential splicing quantifications) differential splicing quantifications 12.7 MB link
ENCFF120KAQ ENCFF120KAQ.tsv tsv (differential expression quantifications) differential expression quantifications 2.9 MB link
ENCFF551OHU ENCFF551OHU.tsv tsv (differential expression quantifications) differential expression quantifications 4.2 MB link
ENCFF878DEU ENCFF878DEU.tsv tsv (differential expression quantifications) differential expression quantifications 1.7 MB link
ENCFF880KEP ENCFF880KEP.tsv tsv (differential expression quantifications) differential expression quantifications 1.9 MB link
ENCFF916EJT ENCFF916EJT.tsv tsv (differential expression quantifications) differential expression quantifications 5.4 MB link
ENCFF330PUT ENCFF330PUT.tsv tsv (gene quantifications) gene quantifications 9.1 MB link
ENCFF344VEU ENCFF344VEU.tsv tsv (gene quantifications) gene quantifications 10.4 MB link
ENCFF563MLA ENCFF563MLA.tsv tsv (gene quantifications) gene quantifications 10.4 MB link
ENCFF582WUD ENCFF582WUD.tsv tsv (gene quantifications) gene quantifications 9.1 MB link
ENCFF633DUC ENCFF633DUC.tsv tsv (gene quantifications) gene quantifications 9.4 MB link
ENCFF794VYD ENCFF794VYD.tsv tsv (gene quantifications) gene quantifications 9.4 MB link
ENCFF118ZUP ENCFF118ZUP.tsv tsv (transcript quantifications) transcript quantifications 29.8 MB link
ENCFF136YBU ENCFF136YBU.tsv tsv (transcript quantifications) transcript quantifications 24.9 MB link
ENCFF234NYQ ENCFF234NYQ.tsv tsv (transcript quantifications) transcript quantifications 27.3 MB link
ENCFF326OER ENCFF326OER.tsv tsv (transcript quantifications) transcript quantifications 29.8 MB link
ENCFF365PWI ENCFF365PWI.tsv tsv (transcript quantifications) transcript quantifications 24.9 MB link
ENCFF682RLJ ENCFF682RLJ.tsv tsv (transcript quantifications) transcript quantifications 25.3 MB link
ENCFF826JDI ENCFF826JDI.tsv tsv (transcript quantifications) transcript quantifications 25.3 MB link
ENCFF930OTO ENCFF930OTO.tsv tsv (transcript quantifications) transcript quantifications 27.3 MB link