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ENCSR284SZO

ENCSR ENCODE
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RNA-seq on HepG2 cells treated with a CRISPR gRNA against EXOSC5. (EXOSC5-BGHcLV07)

Organism: Homo sapiens
Experiment Types:
CRISPR RNA-seq
Status: released
Contact: Brenton Graveley, UConn

Summary

CRISPR RNA-seq of EXOSC5 in Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens EXOSC5

Overall Design

RNA-seq on HepG2 cells treated with a CRISPR gRNA against EXOSC5. (EXOSC5-BGHcLV07)

Analysis page is available for ENCSR284SZO.

Dataset Citations (1)

A large-scale binding and functional map of human RNA-binding proteins.
PMID 32728246 · 2020 · Nature
Eric L Van Nostrand, Peter Freese, Gabriel A Pratt, Xiaofeng Wang, Xintao Wei, Rui Xiao, Steven M Blue, Jia-Yu Chen, Neal A L Cody, Daniel Dominguez, Sara Olson, Balaji Sundararaman, Lijun Zhan, Cassandra Bazile, Louis Philip Benoit Bouvrette, Julie Bergalet, Michael O Duff, Keri E Garcia, Chelsea Gelboin-Burkhart, Myles Hochman, Nicole J Lambert, Hairi Li, Michael P McGurk, Thai B Nguyen, Tsultrim Palden, Ines Rabano, Shashank Sathe, Rebecca Stanton, Amanda Su, Ruth Wang, Brian A Yee, Bing Zhou, Ashley L Louie, Stefan Aigner, Xiang-Dong Fu, Eric Lécuyer, Christopher B Burge, Brenton R Graveley, Gene W Yeo

ENCODE Metadata

Total file size: 72.5 GB
Assay: CRISPR RNA-seq
Target: EXOSC5
Biosample: Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens EXOSC5
Files: 92
Unique ENC file IDs: 92
File Formats
bigWig (48) tsv (19) bam (18) fastq (4) tar (3)
Output types (11)
alignments (12) minus strand signal of all reads (12) minus strand signal of unique reads (12) plus strand signal of all reads (12) plus strand signal of unique reads (12) transcript quantifications (8) gene quantifications (6) transcriptome alignments (6) differential expression quantifications (5) reads (4) differential splicing quantifications (3)

Linked Publications (1)

Data Files (92)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
ENCFF011MSW ENCFF011MSW.bam bam (alignments); assembly: GRCh38; genome annotation: V24 alignments GRCh38 3.0 GB link
ENCFF244FZB ENCFF244FZB.bam bam (alignments); assembly: GRCh38; genome annotation: V24 alignments GRCh38 2.3 GB link
ENCFF473DUQ ENCFF473DUQ.bam bam (alignments); assembly: GRCh38; genome annotation: V24 alignments GRCh38 3.7 GB link
ENCFF533HSQ ENCFF533HSQ.bam bam (alignments); assembly: GRCh38; genome annotation: V24 alignments GRCh38 2.9 GB link
ENCFF055HTJ ENCFF055HTJ.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 2.9 GB link
ENCFF870ZIN ENCFF870ZIN.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 3.7 GB link
ENCFF016GKZ ENCFF016GKZ.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 2.8 GB link
ENCFF346UYL ENCFF346UYL.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 3.5 GB link
ENCFF449RNE ENCFF449RNE.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 2.3 GB link
ENCFF550CTA ENCFF550CTA.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 2.9 GB link
ENCFF583GWU ENCFF583GWU.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 2.3 GB link
ENCFF777VKB ENCFF777VKB.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 2.9 GB link
ENCFF901WES ENCFF901WES.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V24 transcriptome alignments GRCh38 3.7 GB link
ENCFF952LDZ ENCFF952LDZ.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V24 transcriptome alignments GRCh38 4.7 GB link
ENCFF554JWM ENCFF554JWM.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 5.4 GB link
ENCFF681VNB ENCFF681VNB.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 4.3 GB link
ENCFF128OSP ENCFF128OSP.bam bam (transcriptome alignments); assembly: hg19; genome annotation: V19 transcriptome alignments hg19 4.7 GB link
ENCFF631VQP ENCFF631VQP.bam bam (transcriptome alignments); assembly: hg19; genome annotation: V19 transcriptome alignments hg19 3.7 GB link
ENCFF008MLQ ENCFF008MLQ.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V24 minus strand signal of all reads GRCh38 76.1 MB link
ENCFF140ZTO ENCFF140ZTO.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V24 minus strand signal of all reads GRCh38 65.1 MB link
ENCFF360NWL ENCFF360NWL.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V24 minus strand signal of all reads GRCh38 67.3 MB link
ENCFF837WPF ENCFF837WPF.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V24 minus strand signal of all reads GRCh38 58.3 MB link
ENCFF208KZD ENCFF208KZD.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 76.1 MB link
ENCFF606VLZ ENCFF606VLZ.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 65.2 MB link
ENCFF235HZG ENCFF235HZG.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 60.0 MB link
ENCFF416FHU ENCFF416FHU.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 75.3 MB link
ENCFF427SIH ENCFF427SIH.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 55.7 MB link
ENCFF455AIU ENCFF455AIU.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 68.5 MB link
ENCFF639JXD ENCFF639JXD.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 65.1 MB link
ENCFF883UFI ENCFF883UFI.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 64.2 MB link
ENCFF299ZPF ENCFF299ZPF.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 minus strand signal of unique reads GRCh38 58.5 MB link
ENCFF468ATF ENCFF468ATF.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 minus strand signal of unique reads GRCh38 66.7 MB link
ENCFF934GWC ENCFF934GWC.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 minus strand signal of unique reads GRCh38 55.0 MB link
ENCFF934UNG ENCFF934UNG.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 minus strand signal of unique reads GRCh38 63.9 MB link
ENCFF868PEN ENCFF868PEN.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 58.1 MB link
ENCFF874KHJ ENCFF874KHJ.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 66.7 MB link
ENCFF051MJK ENCFF051MJK.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 59.3 MB link
ENCFF303XGI ENCFF303XGI.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 62.5 MB link
ENCFF311IPI ENCFF311IPI.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 51.7 MB link
ENCFF406BDV ENCFF406BDV.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 55.3 MB link
ENCFF888UJW ENCFF888UJW.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 66.0 MB link
ENCFF946JWQ ENCFF946JWQ.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 58.4 MB link
ENCFF020GBZ ENCFF020GBZ.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V24 plus strand signal of all reads GRCh38 77.3 MB link
ENCFF021CFS ENCFF021CFS.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V24 plus strand signal of all reads GRCh38 68.3 MB link
ENCFF435LJM ENCFF435LJM.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V24 plus strand signal of all reads GRCh38 59.1 MB link
ENCFF672QYT ENCFF672QYT.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V24 plus strand signal of all reads GRCh38 66.1 MB link
ENCFF306LRJ ENCFF306LRJ.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 66.1 MB link
ENCFF381ZNO ENCFF381ZNO.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 77.3 MB link
ENCFF146XIP ENCFF146XIP.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 60.9 MB link
ENCFF511VXR ENCFF511VXR.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 69.6 MB link
ENCFF625QIB ENCFF625QIB.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 65.2 MB link
ENCFF763BUS ENCFF763BUS.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 65.9 MB link
ENCFF764FQB ENCFF764FQB.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 76.3 MB link
ENCFF908NLO ENCFF908NLO.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 56.6 MB link
ENCFF035RVA ENCFF035RVA.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 plus strand signal of unique reads GRCh38 68.3 MB link
ENCFF253MBV ENCFF253MBV.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 plus strand signal of unique reads GRCh38 59.7 MB link
ENCFF801DTR ENCFF801DTR.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 plus strand signal of unique reads GRCh38 56.1 MB link
ENCFF912MRS ENCFF912MRS.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 plus strand signal of unique reads GRCh38 65.3 MB link
ENCFF234JVV ENCFF234JVV.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 59.3 MB link
ENCFF722YSS ENCFF722YSS.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 68.3 MB link
ENCFF083KTZ ENCFF083KTZ.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 52.8 MB link
ENCFF203KVV ENCFF203KVV.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 60.8 MB link
ENCFF390BDI ENCFF390BDI.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 65.2 MB link
ENCFF540ZBL ENCFF540ZBL.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 56.6 MB link
ENCFF770ZAJ ENCFF770ZAJ.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 67.5 MB link
ENCFF910AIX ENCFF910AIX.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 59.8 MB link
ENCFF007PXN ENCFF007PXN.fastq fastq (reads) reads 2.1 GB link
ENCFF627DFL ENCFF627DFL.fastq fastq (reads) reads 2.2 GB link
ENCFF660WJN ENCFF660WJN.fastq fastq (reads) reads 1.7 GB link
ENCFF682EZT ENCFF682EZT.fastq fastq (reads) reads 1.7 GB link
ENCFF050JPG ENCFF050JPG.tar tar (differential splicing quantifications); assembly: hg19; genome annotation: V19 differential splicing quantifications hg19 12.2 MB link
ENCFF564IRJ ENCFF564IRJ.tar tar (differential splicing quantifications); assembly: hg19; genome annotation: V19 differential splicing quantifications hg19 2.6 MB link
ENCFF975GDO ENCFF975GDO.tar tar (differential splicing quantifications); assembly: hg19; genome annotation: V19 differential splicing quantifications hg19 2.9 MB link
ENCFF111PSM ENCFF111PSM.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 2.7 MB link
ENCFF175NXT ENCFF175NXT.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 1.9 MB link
ENCFF338PTW ENCFF338PTW.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 4.1 MB link
ENCFF462CCU ENCFF462CCU.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 1.7 MB link
ENCFF824AOC ENCFF824AOC.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 5.6 MB link
ENCFF320ZDH ENCFF320ZDH.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V24 gene quantifications GRCh38 9.4 MB link
ENCFF716UDA ENCFF716UDA.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V24 gene quantifications GRCh38 9.4 MB link
ENCFF355PAP ENCFF355PAP.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF362QHE ENCFF362QHE.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF432BDU ENCFF432BDU.tsv tsv (gene quantifications); assembly: hg19; genome annotation: V19 gene quantifications hg19 9.1 MB link
ENCFF919CQH ENCFF919CQH.tsv tsv (gene quantifications); assembly: hg19; genome annotation: V19 gene quantifications hg19 9.1 MB link
ENCFF381DZK ENCFF381DZK.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V24 transcript quantifications GRCh38 25.3 MB link
ENCFF635XTA ENCFF635XTA.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V24 transcript quantifications GRCh38 25.3 MB link
ENCFF188VWQ ENCFF188VWQ.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.8 MB link
ENCFF313TIL ENCFF313TIL.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.8 MB link
ENCFF412MFU ENCFF412MFU.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.3 MB link
ENCFF822YJD ENCFF822YJD.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.3 MB link
ENCFF776GKA ENCFF776GKA.tsv tsv (transcript quantifications); assembly: hg19; genome annotation: V19 transcript quantifications hg19 25.0 MB link
ENCFF881UQN ENCFF881UQN.tsv tsv (transcript quantifications); assembly: hg19; genome annotation: V19 transcript quantifications hg19 24.9 MB link