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ENCSR521WAI

ENCSR ENCODE
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Control CRISPR gRNA against no target in HepG2 cells followed by RNA-seq. (NT-BGHcLV07)

Organism: Homo sapiens
Experiment Types:
CRISPR RNA-seq
Status: released
Contact: Brenton Graveley, UConn

Summary

CRISPR RNA-seq of in Homo sapiens HepG2 genetically modified (deletion) using CRISPR

Overall Design

Control CRISPR gRNA against no target in HepG2 cells followed by RNA-seq. (NT-BGHcLV07)

Analysis page is available for ENCSR521WAI.

Dataset Citations (1)

A large-scale binding and functional map of human RNA-binding proteins.
PMID 32728246 · 2020 · Nature
Eric L Van Nostrand, Peter Freese, Gabriel A Pratt, Xiaofeng Wang, Xintao Wei, Rui Xiao, Steven M Blue, Jia-Yu Chen, Neal A L Cody, Daniel Dominguez, Sara Olson, Balaji Sundararaman, Lijun Zhan, Cassandra Bazile, Louis Philip Benoit Bouvrette, Julie Bergalet, Michael O Duff, Keri E Garcia, Chelsea Gelboin-Burkhart, Myles Hochman, Nicole J Lambert, Hairi Li, Michael P McGurk, Thai B Nguyen, Tsultrim Palden, Ines Rabano, Shashank Sathe, Rebecca Stanton, Amanda Su, Ruth Wang, Brian A Yee, Bing Zhou, Ashley L Louie, Stefan Aigner, Xiang-Dong Fu, Eric Lécuyer, Christopher B Burge, Brenton R Graveley, Gene W Yeo

ENCODE Metadata

Total file size: 57.3 GB
Assay: CRISPR RNA-seq
Biosample: Homo sapiens HepG2 genetically modified (deletion) using CRISPR
Files: 84
Unique ENC file IDs: 84
File Formats
bigWig (48) bam (18) tsv (14) fastq (4)
Output types (9)
alignments (12) minus strand signal of all reads (12) minus strand signal of unique reads (12) plus strand signal of all reads (12) plus strand signal of unique reads (12) transcript quantifications (8) gene quantifications (6) transcriptome alignments (6) reads (4)

Linked Publications (1)

Data Files (84)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
ENCFF054ESC ENCFF054ESC.bam bam (alignments); assembly: GRCh38; genome annotation: V24 alignments GRCh38 2.2 GB link
ENCFF282YEK ENCFF282YEK.bam bam (alignments); assembly: GRCh38; genome annotation: V24 alignments GRCh38 2.7 GB link
ENCFF867EOY ENCFF867EOY.bam bam (alignments); assembly: GRCh38; genome annotation: V24 alignments GRCh38 2.3 GB link
ENCFF941WYD ENCFF941WYD.bam bam (alignments); assembly: GRCh38; genome annotation: V24 alignments GRCh38 1.9 GB link
ENCFF198HLU ENCFF198HLU.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 2.3 GB link
ENCFF590DAN ENCFF590DAN.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 2.7 GB link
ENCFF100BOV ENCFF100BOV.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 2.6 GB link
ENCFF594ZVR ENCFF594ZVR.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 2.2 GB link
ENCFF729LZA ENCFF729LZA.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 1.8 GB link
ENCFF872YYA ENCFF872YYA.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 2.1 GB link
ENCFF884AVW ENCFF884AVW.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 1.8 GB link
ENCFF944ZZS ENCFF944ZZS.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 2.1 GB link
ENCFF592QPE ENCFF592QPE.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V24 transcriptome alignments GRCh38 3.2 GB link
ENCFF811UHF ENCFF811UHF.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V24 transcriptome alignments GRCh38 3.8 GB link
ENCFF471RIX ENCFF471RIX.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 4.3 GB link
ENCFF509HDB ENCFF509HDB.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 3.6 GB link
ENCFF288KZI ENCFF288KZI.bam bam (transcriptome alignments); assembly: hg19; genome annotation: V19 transcriptome alignments hg19 3.7 GB link
ENCFF716UYP ENCFF716UYP.bam bam (transcriptome alignments); assembly: hg19; genome annotation: V19 transcriptome alignments hg19 3.1 GB link
ENCFF366VEA ENCFF366VEA.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V24 minus strand signal of all reads GRCh38 52.8 MB link
ENCFF617TII ENCFF617TII.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V24 minus strand signal of all reads GRCh38 55.7 MB link
ENCFF749YWN ENCFF749YWN.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V24 minus strand signal of all reads GRCh38 61.7 MB link
ENCFF901IVI ENCFF901IVI.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V24 minus strand signal of all reads GRCh38 47.7 MB link
ENCFF220QSL ENCFF220QSL.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 52.8 MB link
ENCFF550QLM ENCFF550QLM.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 61.8 MB link
ENCFF350FSY ENCFF350FSY.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 48.9 MB link
ENCFF496YUA ENCFF496YUA.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 45.9 MB link
ENCFF597DDG ENCFF597DDG.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 53.1 MB link
ENCFF848CHR ENCFF848CHR.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 53.0 MB link
ENCFF849RWR ENCFF849RWR.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 56.9 MB link
ENCFF983CAO ENCFF983CAO.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 61.3 MB link
ENCFF652YUK ENCFF652YUK.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 minus strand signal of unique reads GRCh38 55.1 MB link
ENCFF911JMW ENCFF911JMW.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 minus strand signal of unique reads GRCh38 52.2 MB link
ENCFF960GZE ENCFF960GZE.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 minus strand signal of unique reads GRCh38 48.0 MB link
ENCFF997PRX ENCFF997PRX.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 minus strand signal of unique reads GRCh38 45.3 MB link
ENCFF279BIT ENCFF279BIT.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 48.0 MB link
ENCFF671FMA ENCFF671FMA.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 54.8 MB link
ENCFF083SGP ENCFF083SGP.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 55.0 MB link
ENCFF295VCC ENCFF295VCC.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 49.2 MB link
ENCFF418EZX ENCFF418EZX.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 42.7 MB link
ENCFF599WBV ENCFF599WBV.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 45.6 MB link
ENCFF694ZMA ENCFF694ZMA.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 52.3 MB link
ENCFF994IJV ENCFF994IJV.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 48.0 MB link
ENCFF790DUO ENCFF790DUO.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V24 plus strand signal of all reads GRCh38 48.5 MB link
ENCFF798HOJ ENCFF798HOJ.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V24 plus strand signal of all reads GRCh38 62.7 MB link
ENCFF865NHP ENCFF865NHP.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V24 plus strand signal of all reads GRCh38 56.6 MB link
ENCFF960VMS ENCFF960VMS.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V24 plus strand signal of all reads GRCh38 53.7 MB link
ENCFF089OMS ENCFF089OMS.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 53.7 MB link
ENCFF167FNY ENCFF167FNY.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 62.8 MB link
ENCFF118MTC ENCFF118MTC.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 49.8 MB link
ENCFF269UQP ENCFF269UQP.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 54.1 MB link
ENCFF427BKX ENCFF427BKX.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 46.7 MB link
ENCFF533DOG ENCFF533DOG.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 53.8 MB link
ENCFF856HUS ENCFF856HUS.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 57.9 MB link
ENCFF890TXB ENCFF890TXB.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 62.1 MB link
ENCFF322DEU ENCFF322DEU.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 plus strand signal of unique reads GRCh38 56.5 MB link
ENCFF549JNP ENCFF549JNP.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 plus strand signal of unique reads GRCh38 46.4 MB link
ENCFF553YAN ENCFF553YAN.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 plus strand signal of unique reads GRCh38 53.5 MB link
ENCFF681UET ENCFF681UET.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 plus strand signal of unique reads GRCh38 49.2 MB link
ENCFF102MCG ENCFF102MCG.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 56.1 MB link
ENCFF226RYE ENCFF226RYE.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 49.2 MB link
ENCFF235GUY ENCFF235GUY.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 43.9 MB link
ENCFF347EXM ENCFF347EXM.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 49.2 MB link
ENCFF369LLY ENCFF369LLY.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 56.4 MB link
ENCFF602TBJ ENCFF602TBJ.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 50.6 MB link
ENCFF706ZTS ENCFF706ZTS.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 46.8 MB link
ENCFF942OYD ENCFF942OYD.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 53.6 MB link
ENCFF100IFZ ENCFF100IFZ.fastq fastq (reads) reads 1.4 GB link
ENCFF230IAI ENCFF230IAI.fastq fastq (reads) reads 1.6 GB link
ENCFF550QYI ENCFF550QYI.fastq fastq (reads) reads 1.6 GB link
ENCFF552VOE ENCFF552VOE.fastq fastq (reads) reads 1.4 GB link
ENCFF154TBF ENCFF154TBF.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V24 gene quantifications GRCh38 9.4 MB link
ENCFF876EFK ENCFF876EFK.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V24 gene quantifications GRCh38 9.4 MB link
ENCFF297PME ENCFF297PME.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF661XEU ENCFF661XEU.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF564PPK ENCFF564PPK.tsv tsv (gene quantifications); assembly: hg19; genome annotation: V19 gene quantifications hg19 9.1 MB link
ENCFF879PGY ENCFF879PGY.tsv tsv (gene quantifications); assembly: hg19; genome annotation: V19 gene quantifications hg19 9.1 MB link
ENCFF468JBD ENCFF468JBD.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V24 transcript quantifications GRCh38 25.3 MB link
ENCFF470GQC ENCFF470GQC.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V24 transcript quantifications GRCh38 25.3 MB link
ENCFF289SVN ENCFF289SVN.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.2 MB link
ENCFF440HPJ ENCFF440HPJ.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.8 MB link
ENCFF662XUE ENCFF662XUE.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.2 MB link
ENCFF853UAP ENCFF853UAP.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.8 MB link
ENCFF687ZKR ENCFF687ZKR.tsv tsv (transcript quantifications); assembly: hg19; genome annotation: V19 transcript quantifications hg19 24.9 MB link
ENCFF718OLD ENCFF718OLD.tsv tsv (transcript quantifications); assembly: hg19; genome annotation: V19 transcript quantifications hg19 24.9 MB link