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ENCSR580TGX

ENCSR ENCODE
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RNA-seq on K562 cells treated with a CRISPR gRNA against POLR2G. (POLR2G-BGKcLV06)

Organism: Homo sapiens
Experiment Types:
CRISPR RNA-seq
Status: released
Contact: Brenton Graveley, UConn

Summary

CRISPR RNA-seq of POLR2G in Homo sapiens K562 genetically modified (deletion) using CRISPR targeting H. sapiens POLR2G

Overall Design

RNA-seq on K562 cells treated with a CRISPR gRNA against POLR2G. (POLR2G-BGKcLV06)

Analysis page is available for ENCSR580TGX.

Dataset Citations (1)

A large-scale binding and functional map of human RNA-binding proteins.
PMID 32728246 · 2020 · Nature
Eric L Van Nostrand, Peter Freese, Gabriel A Pratt, Xiaofeng Wang, Xintao Wei, Rui Xiao, Steven M Blue, Jia-Yu Chen, Neal A L Cody, Daniel Dominguez, Sara Olson, Balaji Sundararaman, Lijun Zhan, Cassandra Bazile, Louis Philip Benoit Bouvrette, Julie Bergalet, Michael O Duff, Keri E Garcia, Chelsea Gelboin-Burkhart, Myles Hochman, Nicole J Lambert, Hairi Li, Michael P McGurk, Thai B Nguyen, Tsultrim Palden, Ines Rabano, Shashank Sathe, Rebecca Stanton, Amanda Su, Ruth Wang, Brian A Yee, Bing Zhou, Ashley L Louie, Stefan Aigner, Xiang-Dong Fu, Eric Lécuyer, Christopher B Burge, Brenton R Graveley, Gene W Yeo

ENCODE Metadata

Total file size: 70.4 GB
Assay: CRISPR RNA-seq
Target: POLR2G
Biosample: Homo sapiens K562 genetically modified (deletion) using CRISPR targeting H. sapiens POLR2G
Files: 92
Unique ENC file IDs: 92
File Formats
bigWig (48) tsv (19) bam (18) fastq (4) tar (3)
Output types (11)
alignments (12) minus strand signal of all reads (12) minus strand signal of unique reads (12) plus strand signal of all reads (12) plus strand signal of unique reads (12) transcript quantifications (8) gene quantifications (6) transcriptome alignments (6) differential expression quantifications (5) reads (4) differential splicing quantifications (3)

Linked Publications (1)

Data Files (92)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
ENCFF472EEK ENCFF472EEK.bam bam (alignments); assembly: GRCh38; genome annotation: V24 alignments GRCh38 2.8 GB link
ENCFF510WYW ENCFF510WYW.bam bam (alignments); assembly: GRCh38; genome annotation: V24 alignments GRCh38 3.7 GB link
ENCFF765BFE ENCFF765BFE.bam bam (alignments); assembly: GRCh38; genome annotation: V24 alignments GRCh38 3.0 GB link
ENCFF835JMY ENCFF835JMY.bam bam (alignments); assembly: GRCh38; genome annotation: V24 alignments GRCh38 2.2 GB link
ENCFF318IDK ENCFF318IDK.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 2.8 GB link
ENCFF594PPJ ENCFF594PPJ.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 3.7 GB link
ENCFF098TQV ENCFF098TQV.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 2.7 GB link
ENCFF213AXP ENCFF213AXP.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 3.6 GB link
ENCFF696YWW ENCFF696YWW.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 2.2 GB link
ENCFF797KWX ENCFF797KWX.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 2.2 GB link
ENCFF857BZH ENCFF857BZH.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 3.0 GB link
ENCFF896RSA ENCFF896RSA.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 3.0 GB link
ENCFF115JUZ ENCFF115JUZ.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V24 transcriptome alignments GRCh38 3.3 GB link
ENCFF862SNC ENCFF862SNC.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V24 transcriptome alignments GRCh38 4.4 GB link
ENCFF562UYT ENCFF562UYT.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 3.8 GB link
ENCFF872MIB ENCFF872MIB.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 5.1 GB link
ENCFF112FPN ENCFF112FPN.bam bam (transcriptome alignments); assembly: hg19; genome annotation: V19 transcriptome alignments hg19 4.4 GB link
ENCFF359OFM ENCFF359OFM.bam bam (transcriptome alignments); assembly: hg19; genome annotation: V19 transcriptome alignments hg19 3.3 GB link
ENCFF011JKW ENCFF011JKW.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V24 minus strand signal of all reads GRCh38 74.7 MB link
ENCFF097CDW ENCFF097CDW.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V24 minus strand signal of all reads GRCh38 85.8 MB link
ENCFF424AUJ ENCFF424AUJ.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V24 minus strand signal of all reads GRCh38 84.4 MB link
ENCFF802OFA ENCFF802OFA.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V24 minus strand signal of all reads GRCh38 96.4 MB link
ENCFF469XHY ENCFF469XHY.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 96.4 MB link
ENCFF860BSO ENCFF860BSO.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 84.4 MB link
ENCFF149RLC ENCFF149RLC.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 70.9 MB link
ENCFF340WVE ENCFF340WVE.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 83.7 MB link
ENCFF397TNH ENCFF397TNH.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 88.3 MB link
ENCFF635GQJ ENCFF635GQJ.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 93.8 MB link
ENCFF743DRA ENCFF743DRA.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 81.6 MB link
ENCFF847WGY ENCFF847WGY.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 74.8 MB link
ENCFF034DZX ENCFF034DZX.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 minus strand signal of unique reads GRCh38 72.2 MB link
ENCFF530YMF ENCFF530YMF.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 minus strand signal of unique reads GRCh38 84.9 MB link
ENCFF608WPQ ENCFF608WPQ.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 minus strand signal of unique reads GRCh38 78.7 MB link
ENCFF718SCG ENCFF718SCG.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 minus strand signal of unique reads GRCh38 67.4 MB link
ENCFF035ACR ENCFF035ACR.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 71.3 MB link
ENCFF043BEL ENCFF043BEL.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 83.7 MB link
ENCFF027XIE ENCFF027XIE.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 63.1 MB link
ENCFF032CSB ENCFF032CSB.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 73.2 MB link
ENCFF541NKZ ENCFF541NKZ.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 71.4 MB link
ENCFF590DLA ENCFF590DLA.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 67.8 MB link
ENCFF919MOW ENCFF919MOW.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 77.6 MB link
ENCFF949KID ENCFF949KID.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 83.6 MB link
ENCFF067DJK ENCFF067DJK.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V24 plus strand signal of all reads GRCh38 75.5 MB link
ENCFF234QEQ ENCFF234QEQ.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V24 plus strand signal of all reads GRCh38 97.6 MB link
ENCFF654AEM ENCFF654AEM.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V24 plus strand signal of all reads GRCh38 86.8 MB link
ENCFF983MYU ENCFF983MYU.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V24 plus strand signal of all reads GRCh38 83.7 MB link
ENCFF407MCU ENCFF407MCU.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 83.7 MB link
ENCFF433QWO ENCFF433QWO.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 97.7 MB link
ENCFF123UKZ ENCFF123UKZ.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 83.0 MB link
ENCFF393CTM ENCFF393CTM.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 89.7 MB link
ENCFF420GDF ENCFF420GDF.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 71.9 MB link
ENCFF734PAT ENCFF734PAT.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 83.0 MB link
ENCFF829VPO ENCFF829VPO.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 95.0 MB link
ENCFF917DIZ ENCFF917DIZ.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 75.9 MB link
ENCFF042DKE ENCFF042DKE.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 plus strand signal of unique reads GRCh38 86.7 MB link
ENCFF171KAY ENCFF171KAY.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 plus strand signal of unique reads GRCh38 68.7 MB link
ENCFF362JSK ENCFF362JSK.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 plus strand signal of unique reads GRCh38 80.2 MB link
ENCFF508SMO ENCFF508SMO.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 plus strand signal of unique reads GRCh38 73.7 MB link
ENCFF270YBV ENCFF270YBV.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 72.8 MB link
ENCFF697HFC ENCFF697HFC.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 85.5 MB link
ENCFF277VIU ENCFF277VIU.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 64.7 MB link
ENCFF301QHY ENCFF301QHY.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 69.5 MB link
ENCFF552YJL ENCFF552YJL.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 73.1 MB link
ENCFF611YVT ENCFF611YVT.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 85.6 MB link
ENCFF739LBE ENCFF739LBE.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 79.5 MB link
ENCFF926RZU ENCFF926RZU.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 75.1 MB link
ENCFF130DUK ENCFF130DUK.fastq fastq (reads) reads 1.5 GB link
ENCFF193JEE ENCFF193JEE.fastq fastq (reads) reads 2.1 GB link
ENCFF476GOQ ENCFF476GOQ.fastq fastq (reads) reads 2.1 GB link
ENCFF981FSJ ENCFF981FSJ.fastq fastq (reads) reads 1.6 GB link
ENCFF596YBC ENCFF596YBC.tar tar (differential splicing quantifications); assembly: hg19; genome annotation: V19 differential splicing quantifications hg19 14.7 MB link
ENCFF902WYP ENCFF902WYP.tar tar (differential splicing quantifications); assembly: hg19; genome annotation: V19 differential splicing quantifications hg19 4.2 MB link
ENCFF945DBK ENCFF945DBK.tar tar (differential splicing quantifications); assembly: hg19; genome annotation: V19 differential splicing quantifications hg19 3.5 MB link
ENCFF246WJO ENCFF246WJO.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 2.7 MB link
ENCFF370BAU ENCFF370BAU.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 4.5 MB link
ENCFF466GJD ENCFF466GJD.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 1.7 MB link
ENCFF475HOO ENCFF475HOO.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 5.7 MB link
ENCFF976BPL ENCFF976BPL.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 2.0 MB link
ENCFF205OKK ENCFF205OKK.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V24 gene quantifications GRCh38 9.4 MB link
ENCFF279NLL ENCFF279NLL.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V24 gene quantifications GRCh38 9.4 MB link
ENCFF561DLX ENCFF561DLX.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF575NQL ENCFF575NQL.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF075LPN ENCFF075LPN.tsv tsv (gene quantifications); assembly: hg19; genome annotation: V19 gene quantifications hg19 9.1 MB link
ENCFF448RPM ENCFF448RPM.tsv tsv (gene quantifications); assembly: hg19; genome annotation: V19 gene quantifications hg19 9.1 MB link
ENCFF344TZO ENCFF344TZO.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V24 transcript quantifications GRCh38 25.3 MB link
ENCFF695UCF ENCFF695UCF.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V24 transcript quantifications GRCh38 25.3 MB link
ENCFF330AQC ENCFF330AQC.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.8 MB link
ENCFF770TNR ENCFF770TNR.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.3 MB link
ENCFF916NIL ENCFF916NIL.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.4 MB link
ENCFF925LZX ENCFF925LZX.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.8 MB link
ENCFF557UNS ENCFF557UNS.tsv tsv (transcript quantifications); assembly: hg19; genome annotation: V19 transcript quantifications hg19 24.9 MB link
ENCFF632MXM ENCFF632MXM.tsv tsv (transcript quantifications); assembly: hg19; genome annotation: V19 transcript quantifications hg19 24.9 MB link